Strain Name:
C57BL/6J-MtgxR4436Btlr/Mmmh
Stock Number:
041702-MU
Citation ID:
RRID:MMRRC_041702-MU
Other Names:
R4436 (G1), C57BL/6J-MtgxR4436Btlr
Major Collection:

Gene Information

Cdk17
Name: cyclin-dependent kinase 17
Synonyms: Pctk2, 6430598J10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 237459
VEGA: 10
HGNC: HGNC:8750
Homologene: 55666
Nes
Name: nestin
Synonyms: RC2, Ifaprc2, ESTM46, Marc2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 18008
HGNC: HGNC:7756
Homologene: 136487
Cd86
Name: CD86 antigen
Synonyms: B7-2, B7.2, MB7-2, B70, Ly-58, Cd28l2, Ly58
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 12524
HGNC: HGNC:1705
Homologene: 10443
Macf1
Name: microtubule-actin crosslinking factor 1
Synonyms: Aclp7, Acf7, trabeculin alpha
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 11426
Homologene: 136191
L3mbtl2
Name: L3MBTL2 polycomb repressive complex 1 subunit
Synonyms: m4mbt, 4732493N06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 214669
Homologene: 12882
Ltn1
Name: listerin E3 ubiquitin protein ligase 1
Synonyms: Zfp294, 4930528H02Rik, Rnf160, Listerin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 78913
VEGA: 16
Homologene: 32272
Asap1
Name: ArfGAP with SH3 domain, ankyrin repeat and PH domain1
Synonyms: Ddef1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 13196
HGNC: HGNC:2720
Homologene: 7684
Pi4ka
Name: phosphatidylinositol 4-kinase alpha
Synonyms: Pik4ca
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 224020
HGNC: HGNC:8983
Homologene: 11171
Xpo7
Name: exportin 7
Synonyms: Ranbp16, 4930506C02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 65246
VEGA: 14
Homologene: 22857
Siah1b
Name: siah E3 ubiquitin protein ligase 1B
Synonyms: Sinh1b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
Alteration at locus: Chemically Induced
NCBI: 20438
Homologene: 56441
Ascc2
Name: activating signal cointegrator 1 complex subunit 2
Synonyms: ASC1p100, 1700011I11Rik, 2610034L15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 75452
Homologene: 41774
Ppp1r15a
Name: protein phosphatase 1, regulatory subunit 15A
Synonyms: Gadd34, Myd116
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 17872
Homologene: 8639
Cemip
Name: cell migration inducing protein, hyaluronan binding
Synonyms: 9930013L23Rik, 6330404C01Rik, 12H19.01.T7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 80982
Homologene: 10268
Ddx24
Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 24
Synonyms: 1700055J08Rik, 2510027P10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 27225
VEGA: 12
Homologene: 10702
March7
Name: membrane-associated ring finger (C3HC4) 7
Synonyms: Axot, Gtrgeo17
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 57438
Homologene: 10753
Clec4a2
Name: C-type lectin domain family 4, member a2
Synonyms: Dcir1, Clecsf6, DCIR, dendritic cell immunoreceptor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 26888
Homologene: 49291
Esyt3
Name: extended synaptotagmin-like protein 3
Synonyms: D9Ertd280e, Fam62c
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 272636
Homologene: 18626
Rac2
Name: Rac family small GTPase 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 19354
VEGA: 15
HGNC: HGNC:9802
Homologene: 55699
Ttn
Name: titin
Synonyms: shru, connectin, mdm, 2310057K23Rik, D330041I19Rik, 1100001C23Rik, D830007G01Rik, 2310036G12Rik, L56, 2310074I15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 22138
Homologene: 130650
Fat2
Name: FAT atypical cadherin 2
Synonyms: Fath2, mKIAA0811, LOC245827, EMI2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 245827
HGNC: HGNC:3596
Homologene: 1110
Aff3
Name: AF4/FMR2 family, member 3
Synonyms: 3222402O04Rik, Laf4, LAF-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 16764
HGNC: HGNC:6473
Homologene: 1718
Hivep2
Name: human immunodeficiency virus type I enhancer binding protein 2
Synonyms: MIBP1, Gm20114, Shn-2, Schnurri-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 15273
HGNC: HGNC:4921
Homologene: 4900
Plek
Name: pleckstrin
Synonyms: 2010300B13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 56193
HGNC: HGNC:9070
Homologene: 2000
Tubd1
Name: tubulin, delta 1
Synonyms: 4930550G19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 56427
Homologene: 9443
Gm8251
Name: predicted gene 8251
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 102636082
VEGA: 1
Homologene: 141149
Adamts16
Name: a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 16
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 271127
Homologene: 15146
Spic
Name: Spi-C transcription factor (Spi-1/PU.1 related)
Synonyms: Prf, C76795, Spi-C
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 20728
Homologene: 7953
Gtf2ird2
Name: GTF2I repeat domain containing 2
Synonyms: 1700012P16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 114674
Homologene: 24941
Ggn
Name: gametogenetin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 243897
Homologene: 45139
Vmn2r94
Name: vomeronasal 2, receptor 94
Synonyms: EG665227
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 665227
Homologene: 129751
Grm8
Name: glutamate receptor, metabotropic 8
Synonyms: Gprc1h, mGluR8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 14823
HGNC: HGNC:4600
Homologene: 654
Stac
Name: src homology three (SH3) and cysteine rich domain
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 20840
Homologene: 2371
Krt77
Name: keratin 77
Synonyms: 4732484G22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 406220
Homologene: 45490
Zswim3
Name: zinc finger SWIM-type containing 3
Synonyms: 4921517A06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 67538
Homologene: 15436
Hivep3
Name: human immunodeficiency virus type I enhancer binding protein 3
Synonyms: Krc, E030045D18Rik, Schnurri-3, 2900056N03Rik, Shn3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 16656
Homologene: 7803
Apopt1
Name: apoptogenic, mitochondrial 1
Synonyms: Apop-1, 2810002N01Rik, 1700081D05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 68020
Homologene: 12225
Mrvi1
Name: MRV integration site 1
Synonyms: Ris1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 17540
HGNC: HGNC:7237
Homologene: 4425
Olfr725
Name: olfactory receptor 725
Synonyms: MOR246-7_p, MOR246-3, GA_x6K02T2PMLR-5725741-5724776
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 258314
Homologene: 44957
Ccdc85a
Name: coiled-coil domain containing 85A
Synonyms: E030025D05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 216613
Homologene: 65605
Coq9
Name: coenzyme Q9
Synonyms: 2310005O14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 67914
Homologene: 6477
Ssh3
Name: slingshot protein phosphatase 3
Synonyms: SSH-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 245857
VEGA: 19
Homologene: 32372
Zfp316
Name: zinc finger protein 316
Synonyms: Emzf1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 54201
Homologene: 69231
Vmn1r17
Name: vomeronasal 1 receptor 17
Synonyms: V1rc16
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 171189
Homologene: 115643
Gm11568
Name: predicted gene 11568
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 432600
Ppp1r3e
Name: protein phosphatase 1, regulatory subunit 3E
Synonyms: LOC382898, A630071A11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 105651
Homologene: 34797
Ces2f
Name: carboxylesterase 2F
Synonyms: 2310038E17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 71903
HGNC: HGNC:1864
Homologene: 135673
Bcl2a1d
Name: B cell leukemia/lymphoma 2 related protein A1d
Synonyms: A1-d
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 12047
HGNC: HGNC:991
Homologene: 2988
Gpr20
Name: G protein-coupled receptor 20
Synonyms: A430106B11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 239530
HGNC: HGNC:4475
Homologene: 3870
Tnfrsf4
Name: tumor necrosis factor receptor superfamily, member 4
Synonyms: ACT35, Txgp1, CD134, Ox40
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 22163
Homologene: 2496
Ikbip
Name: IKBKB interacting protein
Synonyms: 1700023M03Rik, 1200009F10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 67454
Homologene: 12138
Igkv6-20
Name: immunoglobulin kappa variable 6-20
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 108024
Gm6931
Name: predicted gene 6931
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 628903
Olfr753-ps1
Name: olfactory receptor 753, pseudogene 1
Synonyms: GA_x6K02T2PSCP-1611898-1610960, MOR256-38
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 258052
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 38,209,687 bp
  • T to C, chromosome 1 at 44,056,116 bp
  • T to C, chromosome 2 at 60,234,899 bp
  • T to C, chromosome 2 at 76,764,909 bp
  • T to A, chromosome 2 at 164,820,643 bp
  • T to C, chromosome 3 at 87,973,096 bp
  • C to T, chromosome 4 at 120,095,923 bp
  • T to C, chromosome 4 at 123,527,342 bp
  • T to G, chromosome 4 at 156,008,947 bp
  • T to C, chromosome 5 at 134,194,969 bp
  • C to A, chromosome 5 at 143,254,048 bp
  • A to T, chromosome 6 at 27,761,238 bp
  • G to C, chromosome 6 at 57,360,734 bp
  • A to G, chromosome 6 at 70,336,120 bp
  • G to A, chromosome 6 at 123,128,054 bp
  • A to T, chromosome 7 at 29,171,551 bp
  • C to T, chromosome 7 at 45,524,779 bp
  • T to C, chromosome 7 at 83,987,429 bp
  • T to C, chromosome 7 at 110,876,917 bp
  • T to C, chromosome 8 at 94,853,115 bp
  • G to A, chromosome 8 at 104,953,156 bp
  • A to T, chromosome 9 at 88,731,700 bp
  • A to T, chromosome 9 at 99,358,025 bp
  • T to C, chromosome 9 at 111,635,008 bp
  • C to A, chromosome 10 at 14,128,969 bp
  • T to A, chromosome 10 at 88,676,955 bp
  • T to A, chromosome 10 at 91,101,889 bp
  • T to A, chromosome 10 at 93,211,896 bp
  • A to G, chromosome 11 at 4,656,305 bp
  • A to G, chromosome 11 at 16,992,972 bp
  • T to C, chromosome 11 at 28,576,457 bp
  • C to A, chromosome 11 at 55,296,198 bp
  • C to T, chromosome 11 at 86,548,919 bp
  • T to A, chromosome 11 at 99,858,595 bp
  • C to A, chromosome 12 at 103,423,974 bp
  • T to A, chromosome 12 at 111,751,208 bp
  • G to A, chromosome 13 at 70,779,518 bp
  • T to C, chromosome 14 at 50,034,830 bp
  • C to A, chromosome 14 at 54,876,550 bp
  • G to A, chromosome 14 at 70,669,429 bp
  • T to G, chromosome 15 at 64,349,843 bp
  • C to T, chromosome 15 at 73,695,800 bp
  • G to T, chromosome 15 at 78,570,743 bp
  • T to C, chromosome 15 at 81,676,892 bp
  • A to T, chromosome 15 at 101,865,469 bp
  • T to C, chromosome 16 at 17,282,382 bp
  • T to C, chromosome 16 at 36,620,832 bp
  • T to A, chromosome 16 at 49,424,862 bp
  • A to T, chromosome 16 at 87,405,614 bp
  • T to C, chromosome 17 at 18,258,383 bp
  • A to G, chromosome 17 at 37,169,836 bp
  • A to G, chromosome 19 at 4,265,366 bp
  • G to A, chromosome X at 164,071,692 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4436 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041702-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.