Strain Name:
C57BL/6J-MtgxR4471Btlr/Mmmh
Stock Number:
041728-MU
Citation ID:
RRID:MMRRC_041728-MU
Other Names:
R4471 (G1), C57BL/6J-MtgxR4471Btlr
Major Collection:

Gene Information

Rest
Name: RE1-silencing transcription factor
Synonyms: 2610008J04Rik, NRSF
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 19712
HGNC: HGNC:9966
Homologene: 4099
Myh7
Name: myosin, heavy polypeptide 7, cardiac muscle, beta
Synonyms: Myhc-b, B-MHC, MyHC-I, beta-MHC, MYH-beta/slow, Myhcb, betaMHC
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 140781
HGNC: HGNC:7577
Homologene: 68044
Tmem59l
Name: transmembrane protein 59-like
Synonyms: 5330410G16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 67937
Homologene: 8104
Fam110b
Name: family with sequence similarity 110, member B
Synonyms: 1700012H17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 242297
Homologene: 17518
Slc16a3
Name: solute carrier family 16 (monocarboxylic acid transporters), member 3
Synonyms: MCT4, MCT3, monocarboxylate transporter 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 80879
Homologene: 37900
Slk
Name: STE20-like kinase
Synonyms: 9A2, Etk4, SLK, Stk2, mSLK
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 20874
Homologene: 22515
Ulk1
Name: unc-51 like kinase 1
Synonyms: Unc51.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 22241
Homologene: 2640
Rbm6
Name: RNA binding motif protein 6
Synonyms: NY-LU-12, g16, Def-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 19654
HGNC: HGNC:9903
Homologene: 31336
Wdr59
Name: WD repeat domain 59
Synonyms: 5430401O09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 319481
Homologene: 38685
Patj
Name: PATJ, crumbs cell polarity complex component
Synonyms: Inadl, Cipp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 12695
Homologene: 72199
Snrnp200
Name: small nuclear ribonucleoprotein 200 (U5)
Synonyms: U5-200KD, HELIC2, Ascc3l1, A330064G03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 320632
Homologene: 5859
Rexo1
Name: REX1, RNA exonuclease 1
Synonyms: 2610511M11Rik, Rex1, Tceb3bp1, 1700021P10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 66932
Homologene: 41391
Mdn1
Name: midasin AAA ATPase 1
Synonyms: LOC213784, 4833432B22Rik, D4Abb1e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 100019
Homologene: 39689
Nemf
Name: nuclear export mediator factor
Synonyms: 4933405E14Rik, Sdccag1, 1500011I12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 66244
VEGA: 12
Homologene: 3458
Taf2
Name: TATA-box binding protein associated factor 2
Synonyms: 4732460C16Rik, TAF2B, CIF150, TAFII150, 150kDa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 319944
VEGA: 15
Homologene: 31137
Git1
Name: GIT ArfGAP 1
Synonyms: Cat-1, p95Cat
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 216963
HGNC: HGNC:4272
Homologene: 32204
Zzef1
Name: zinc finger, ZZ-type with EF hand domain 1
Synonyms: C130099L13Rik, 8430405D05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 195018
Homologene: 9027
Gpatch2
Name: G patch domain containing 2
Synonyms: Gpatc2, 5830436K05Rik, 5830433G22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 67769
Homologene: 9976
Unc45a
Name: unc-45 myosin chaperone A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 101869
Homologene: 32423
Utp25
Name: UTP25 small subunit processome component
Synonyms: AA408296, Diexf
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 215193
Homologene: 6170
Hipk2
Name: homeodomain interacting protein kinase 2
Synonyms: B230339E18Rik, 1110014O20Rik, Stank
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 15258
Homologene: 68766
Icam5
Name: intercellular adhesion molecule 5, telencephalin
Synonyms: CD50, TLN, Tlcn, Icam3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 15898
VEGA: 9
HGNC: HGNC:5348
Homologene: 2447
Ern1
Name: endoplasmic reticulum (ER) to nucleus signalling 1
Synonyms: 9030414B18Rik, Ire1a, Ire1alpha, Ire1p
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 78943
HGNC: HGNC:3449
Homologene: 55580
Lman1
Name: lectin, mannose-binding, 1
Synonyms: F5F8D, ERGIC53, MCFD1, gp58, MR60, 2610020P13Rik, P58
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 70361
HGNC: HGNC:6631
Homologene: 4070
Ttc23
Name: tetratricopeptide repeat domain 23
Synonyms: 1600012K10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 67009
Homologene: 11289
Syt17
Name: synaptotagmin XVII
Synonyms: Bk
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 110058
Homologene: 9553
Cpne6
Name: copine VI
Synonyms: neuronal copine
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 12891
HGNC: HGNC:2319
Homologene: 81815
Nktr
Name: natural killer tumor recognition sequence
Synonyms: D9Wsu172e, 5330401F18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 18087
HGNC: HGNC:7833
Homologene: 122148
Tln1
Name: talin 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 21894
Homologene: 21267
R3hcc1l
Name: R3H domain and coiled-coil containing 1 like
Synonyms: D19Ertd386e, 1700036B12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 52013
Homologene: 8707
Zfp979
Name: zinc finger protein 979
Synonyms: 2610305D13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 112422
Homologene: 133076
Hydin
Name: HYDIN, axonemal central pair apparatus protein
Synonyms: 4930545D19Rik, hyrh, hy-3, 1700034M11Rik, hy3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 244653
Homologene: 52118
Cmya5
Name: cardiomyopathy associated 5
Synonyms: 2310076E21Rik, 2310076E16Rik, Myospryn
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 76469
VEGA: 13
Homologene: 137367
Eif3f
Name: eukaryotic translation initiation factor 3, subunit F
Synonyms: Eif3s5, 0610037M02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 66085
HGNC: HGNC:3275
Homologene: 2783
Rin2
Name: Ras and Rab interactor 2
Synonyms: 2010003K16Rik, 4632403N06Rik, RASSF4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 74030
Homologene: 32430
Eml1
Name: echinoderm microtubule associated protein like 1
Synonyms: ELP79, heco, 1110008N23Rik, A930030P13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 68519
HGNC: HGNC:3330
Homologene: 20931
Ube2u
Name: ubiquitin-conjugating enzyme E2U (putative)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 381534
Homologene: 17594
Impdh1
Name: inosine monophosphate dehydrogenase 1
Synonyms: B930086D20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 23917
HGNC: HGNC:6052
Homologene: 68096
Tecrl
Name: trans-2,3-enoyl-CoA reductase-like
Synonyms: Srd5a2l2, D330017N19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 243078
Homologene: 27549
Nrxn3
Name: neurexin III
Synonyms: 4933401A11Rik, neurexin III beta, D12Bwg0831e, neurexin III beta, 9330112C09Rik, neurexin III alpha, neurexin III alpha
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 18191
HGNC: HGNC:8010
Homologene: 83225
Ivns1abp
Name: influenza virus NS1A binding protein
Synonyms: HSPC068, 1190004M08Rik, ND1, NS-1, Nd1-L, Nd1-S, NS1-BP, 1700126I16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 117198
Homologene: 31384
Cdh2
Name: cadherin 2
Synonyms: N-cadherin, N-CAD, Ncad
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 12558
HGNC: HGNC:1759
Homologene: 20424
Ankdd1a
Name: ankyrin repeat and death domain containing 1A
Synonyms: LOC384945, EG330963
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 330963
VEGA: 9
Homologene: 65619
Smarca2
Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2
Synonyms: 2610209L14Rik, Snf2l2, brm
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 67155
Homologene: 2308
4930452B06Rik
Name: RIKEN cDNA 4930452B06 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 74430
Homologene: 18873
Sox5
Name: SRY (sex determining region Y)-box 5
Synonyms: A730017D01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 20678
Homologene: 21378
Zfp777
Name: zinc finger protein 777
Synonyms: 2500002G23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 72306
Homologene: 56721
Ube4a
Name: ubiquitination factor E4A
Synonyms: 4732444G18Rik, UFD2b, 9930123J21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 140630
Homologene: 3517
Slc5a4b
Name: solute carrier family 5 (neutral amino acid transporters, system A), member 4b
Synonyms: 2010104G07Rik, SGLT3b, pSGLT2, SAAT1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 64454
Homologene: 56968
Cyp2j12
Name: cytochrome P450, family 2, subfamily j, polypeptide 12
Synonyms: OTTMUSG00000007939, Cyp2j12-ps
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 242546
Homologene: 133195
Fn3krp
Name: fructosamine 3 kinase related protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 238024
Homologene: 11093
Ankrd13b
Name: ankyrin repeat domain 13b
Synonyms: B930093C12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 268445
Homologene: 27367
Rbm44
Name: RNA binding motif protein 44
Synonyms: LOC329207
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 329207
Homologene: 66636
Ttl
Name: tubulin tyrosine ligase
Synonyms: 2700049H19Rik, 2410003M22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 69737
Homologene: 32678
Mtif2
Name: mitochondrial translational initiation factor 2
Synonyms: 2410112O06Rik, IF-2mt, 2310038D14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 76784
HGNC: HGNC:7441
Homologene: 1840
Alpk1
Name: alpha-kinase 1
Synonyms: 8430410J10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 71481
Homologene: 11849
Ceacam1
Name: carcinoembryonic antigen-related cell adhesion molecule 1
Synonyms: Mhv-1, Cea1, mmCGM1, MHVR1, Cea-1, Cea7, mmCGM2, Bgp1, CD66a, Bgp, Cc1, Hv2, Cea-7, C-CAM, mCEA1, Hv-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 26365
HGNC: HGNC:1814
Homologene: 128630
Olfr26
Name: olfactory receptor 26
Synonyms: GA_x6K02T2PVTD-32550930-32551856, MOR171-9, MTPCR09
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 18324
VEGA: 9
HGNC: HGNC:8481
Homologene: 79676
Asah1
Name: N-acylsphingosine amidohydrolase 1
Synonyms: acid ceramidase, 2310081N20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 11886
HGNC: HGNC:735
Homologene: 10504
Celf3
Name: CUGBP, Elav-like family member 3
Synonyms: BRUNOL1, 4930415M08Rik, CAGH4, ERDA4, Tnrc4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 78784
Homologene: 48510
AF067061
Name: cDNA sequence AF067061
Synonyms: clone L4 variable group of 2-cell-stage gene family, clone L8 variable group of 2-cell-stage gene family
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 236546
Zfp24
Name: zinc finger protein 24
Synonyms: 5033419P20Rik, 3526401F17Rik, KOX17, ZF-12, Zfp191
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 59057
Homologene: 5068
Glrp1
Name: glutamine repeat protein 1
Synonyms: GRP-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 14659
Cdc34b
Name: cell division cycle 34B
Synonyms: Cdc34-ps
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 111266
Prss2
Name: protease, serine 2
Synonyms: TRY8, TRYP, Tesp4, Try2, Ta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 22072
HGNC: HGNC:9483
Homologene: 122141
Msc
Name: musculin
Synonyms: bHLHa22, MyoR
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 17681
HGNC: HGNC:7321
Homologene: 3738
Dnajb4
Name: DnaJ heat shock protein family (Hsp40) member B4
Synonyms: 5730460G06Rik, 1700029A20Rik, 2010306G19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 67035
Homologene: 100610
Pagr1b
Name: PAXIP1 associated glutamate rich protein 1B
Synonyms: Gm20418
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to C, chromosome 1 at 14,755,678 bp
  • T to A, chromosome 1 at 88,503,474 bp
  • T to A, chromosome 1 at 91,169,053 bp
  • C to T, chromosome 1 at 151,361,239 bp
  • A to G, chromosome 1 at 187,233,140 bp
  • C to T, chromosome 1 at 193,130,137 bp
  • T to A, chromosome 2 at 127,238,753 bp
  • T to C, chromosome 2 at 129,082,057 bp
  • C to T, chromosome 2 at 145,860,446 bp
  • A to G, chromosome 3 at 94,488,278 bp
  • A to G, chromosome 3 at 127,679,526 bp
  • A to T, chromosome 3 at 152,185,162 bp
  • A to T, chromosome 4 at 5,799,092 bp
  • A to G, chromosome 4 at 32,668,860 bp
  • A to T, chromosome 4 at 43,551,018 bp
  • A to G, chromosome 4 at 96,133,069 bp
  • A to G, chromosome 4 at 98,535,579 bp
  • G to A, chromosome 4 at 100,481,646 bp
  • A to T, chromosome 4 at 147,613,456 bp
  • T to C, chromosome 5 at 77,281,180 bp
  • A to G, chromosome 5 at 83,313,287 bp
  • C to T, chromosome 5 at 110,789,357 bp
  • G to A, chromosome 6 at 29,204,632 bp
  • T to A, chromosome 6 at 38,736,922 bp
  • A to G, chromosome 6 at 41,522,846 bp
  • A to T, chromosome 6 at 48,042,107 bp
  • A to G, chromosome 6 at 143,844,765 bp
  • T to A, chromosome 7 at 25,474,600 bp
  • G to A, chromosome 7 at 67,670,156 bp
  • A to G, chromosome 7 at 80,332,980 bp
  • T to C, chromosome 7 at 108,940,946 bp
  • A to G, chromosome 7 at 118,436,817 bp
  • C to T, chromosome 7 at 127,001,958 bp
  • A to G, chromosome 8 at 41,343,724 bp
  • A to G, chromosome 8 at 70,487,301 bp
  • A to G, chromosome 8 at 110,587,132 bp
  • A to C, chromosome 8 at 111,466,787 bp
  • T to A, chromosome 9 at 21,035,506 bp
  • T to C, chromosome 9 at 38,855,631 bp
  • A to T, chromosome 9 at 44,946,532 bp
  • T to C, chromosome 9 at 65,503,509 bp
  • T to C, chromosome 9 at 107,852,146 bp
  • T to C, chromosome 9 at 121,748,896 bp
  • G to A, chromosome 10 at 76,058,891 bp
  • A to T, chromosome 10 at 80,542,658 bp
  • T to C, chromosome 11 at 29,540,053 bp
  • T to A, chromosome 11 at 72,913,331 bp
  • T to A, chromosome 11 at 77,476,214 bp
  • A to G, chromosome 11 at 77,499,824 bp
  • A to G, chromosome 11 at 94,742,238 bp
  • C to A, chromosome 11 at 106,420,042 bp
  • T to C, chromosome 11 at 120,955,948 bp
  • T to G, chromosome 11 at 121,426,673 bp
  • G to A, chromosome 12 at 69,314,442 bp
  • T to C, chromosome 12 at 90,204,741 bp
  • T to C, chromosome 12 at 108,506,635 bp
  • A to T, chromosome 13 at 93,092,325 bp
  • G to T, chromosome 13 at 120,264,043 bp
  • A to G, chromosome 14 at 8,536,571 bp
  • A to T, chromosome 14 at 54,991,854 bp
  • G to A, chromosome 14 at 55,516,632 bp
  • T to C, chromosome 15 at 55,058,880 bp
  • T to C, chromosome 18 at 16,774,476 bp
  • A to G, chromosome 18 at 24,018,115 bp
  • A to T, chromosome 18 at 65,991,726 bp
  • T to A, chromosome 19 at 26,619,877 bp
  • T to C, chromosome 19 at 42,582,820 bp
  • T to G, chromosome 19 at 47,615,423 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4471 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041728-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.