Strain Name:
C57BL/6J-MtgxR4533Btlr/Mmmh
Stock Number:
041773-MU
Citation ID:
RRID:MMRRC_041773-MU
Other Names:
R4533 (G1), C57BL/6J-MtgxR4533Btlr
Major Collection:

Gene Information

Dbh
Name: dopamine beta hydroxylase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 13166
HGNC: HGNC:2689
Homologene: 615
Kcmf1
Name: potassium channel modulatory factor 1
Synonyms: Pmcf, 1700094M07Rik, clone DEBT-91
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 74287
Homologene: 99845
Tiparp
Name: TCDD-inducible poly(ADP-ribose) polymerase
Synonyms: PARP-7, DDF1, PARP7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 99929
Homologene: 9167
Zfp609
Name: zinc finger protein 609
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 214812
Homologene: 72220
Ttc3
Name: tetratricopeptide repeat domain 3
Synonyms: TPRD, D16Ium21e, 2610202A04Rik, D16Ium21
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 22129
Homologene: 2487
Orc4
Name: origin recognition complex, subunit 4
Synonyms: mMmORC4, Orc4P, Orc4l, Orc4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 26428
HGNC: HGNC:8490
Homologene: 8059
Anapc5
Name: anaphase-promoting complex subunit 5
Synonyms: 2510006G12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 59008
Homologene: 41118
Ubr1
Name: ubiquitin protein ligase E3 component n-recognin 1
Synonyms: E3 alpha
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 22222
Homologene: 7582
Ago3
Name: argonaute RISC catalytic subunit 3
Synonyms: eIF2C3, C130014L07Rik, argonaute 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 214150
Homologene: 49799
Akap9
Name: A kinase (PRKA) anchor protein (yotiao) 9
Synonyms: mei2-5, G1-448-15, 5730481H23Rik, AKAP450, repro12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 100986
HGNC: HGNC:379
Homologene: 134583
Heatr1
Name: HEAT repeat containing 1
Synonyms: B130016L12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 217995
VEGA: 13
Homologene: 34562
Plekha5
Name: pleckstrin homology domain containing, family A member 5
Synonyms: Ayu21-9, Gt(Ayu21)9Imeg, AMH-Cre, PEPP2, 2810431N21Rik, Tg(AMH-cre)1Flor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 109135
Homologene: 10377
Slc12a3
Name: solute carrier family 12, member 3
Synonyms: TSC, NCC
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 20497
Homologene: 287
Wdr12
Name: WD repeat domain 12
Synonyms: Ytm1, Ytm1p, 4933402C23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 57750
Homologene: 10098
Nek1
Name: NIMA (never in mitosis gene a)-related expressed kinase 1
Synonyms: kat, kidney, anemia and testis, D8Ertd790e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 18004
HGNC: HGNC:7744
Homologene: 14376
Lin54
Name: lin-54 homolog (C. elegans)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 231506
Homologene: 18212
Ptprj
Name: protein tyrosine phosphatase, receptor type, J
Synonyms: Byp, CD148, DEP-1, RPTPJ, Scc-1, Scc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 19271
HGNC: HGNC:9673
Homologene: 2130
Ercc1
Name: excision repair cross-complementing rodent repair deficiency, complementation group 1
Synonyms: Ercc-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 13870
HGNC: HGNC:3433
Homologene: 1501
Ccdc171
Name: coiled-coil domain containing 171
Synonyms: 4930418J05Rik, A330015D16Rik, 4930473A06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 320226
Homologene: 27942
Zmiz1
Name: zinc finger, MIZ-type containing 1
Synonyms: Rai17, Zimp10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 328365
Homologene: 10667
Csmd1
Name: CUB and Sushi multiple domains 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 94109
Homologene: 69536
Ptchd3
Name: patched domain containing 3
Synonyms: 4933440L20Rik, 4930451E13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 74675
Homologene: 111041
Plcb3
Name: phospholipase C, beta 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 18797
VEGA: 19
HGNC: HGNC:9056
Homologene: 47960
Adamts7
Name: a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 7
Synonyms: ADAM-TS7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 108153
HGNC: HGNC:223
Homologene: 22803
Mrpl44
Name: mitochondrial ribosomal protein L44
Synonyms: 5730593H20Rik, 1810030E18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 69163
Homologene: 11293
Tbc1d9
Name: TBC1 domain family, member 9
Synonyms: C76116, 4933431N12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 71310
Homologene: 57079
Vmn2r72
Name: vomeronasal 2, receptor 72
Synonyms: Vmn2r72-ps, EG244114
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 244114
Homologene: 115466
Rufy4
Name: RUN and FYVE domain containing 4
Synonyms: F930048N03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 435626
Homologene: 52359
Vwc2l
Name: von Willebrand factor C domain-containing protein 2-like
Synonyms: brorin-like, Brl, A830006F12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 320460
Homologene: 27945
Tex14
Name: testis expressed gene 14
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 83560
Homologene: 12838
Raly
Name: hnRNP-associated with lethal yellow
Synonyms: Merc
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 19383
Homologene: 7216
Mast1
Name: microtubule associated serine/threonine kinase 1
Synonyms: SAST, SAST170, 9430008B02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 56527
Homologene: 10543
Myo18b
Name: myosin XVIIIb
Synonyms: 4933411E19Rik, 4932408L24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 74376
Homologene: 53435
Gm12185
Name: predicted gene 12185
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 620913
Homologene: 83188
Uap1
Name: UDP-N-acetylglucosamine pyrophosphorylase 1
Synonyms: ESTM38, SPAG2, AgX, AGX1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 107652
Homologene: 2342
Vmn1r77
Name: vomeronasal 1 receptor 77
Synonyms: Gm6935
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 628946
Homologene: 103843
Itga3
Name: integrin alpha 3
Synonyms: VLA-3 alpha 3, alpha3-integrin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 16400
HGNC: HGNC:6139
Homologene: 21129
Omt2b
Name: oocyte maturation, beta
Synonyms: OM2b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 382088
Homologene: 136010
Rnf217
Name: ring finger protein 217
Synonyms: Ibrdc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 268291
Homologene: 66368
Olfr726
Name: olfactory receptor 726
Synonyms: GA_x6K02T2PMLR-5775299-5774334, MOR246-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 258313
Homologene: 44957
Art5
Name: ADP-ribosyltransferase 5
Synonyms: Yac-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 11875
Homologene: 7231
Crtc3
Name: CREB regulated transcription coactivator 3
Synonyms: 2610312F20Rik, 6332415K15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 70461
Homologene: 11248
Blvra
Name: biliverdin reductase A
Synonyms: Blvr, 0610006A11Rik, 2500001N03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 109778
HGNC: HGNC:1062
Homologene: 572
Acot8
Name: acyl-CoA thioesterase 8
Synonyms: Pte1, PTE-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 170789
Homologene: 3991
Fam122c
Name: family with sequence similarity 122, member C
Synonyms: 4930527G05Rik, 4930432H15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
Alteration at locus: Chemically Induced
NCBI: 73866
Homologene: 87062
Leprel2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
Pcdha7
Name: protocadherin alpha 7
Synonyms: Cnr4, Crnr4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 12939
HGNC: HGNC:8673
Homologene: 117962
Ighv1-37
Name: immunoglobulin heavy variable 1-37
Synonyms: Gm16716
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 668517
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 60,078,195 bp
  • G to A, chromosome 1 at 70,882,139 bp
  • T to C, chromosome 1 at 74,147,663 bp
  • T to C, chromosome 1 at 79,776,254 bp
  • A to T, chromosome 1 at 170,143,425 bp
  • C to A, chromosome 2 at 27,177,331 bp
  • G to A, chromosome 2 at 48,937,489 bp
  • C to T, chromosome 2 at 90,439,955 bp
  • T to C, chromosome 2 at 120,942,482 bp
  • T to A, chromosome 2 at 127,090,384 bp
  • A to T, chromosome 2 at 154,865,933 bp
  • A to G, chromosome 2 at 164,799,857 bp
  • A to G, chromosome 3 at 65,546,347 bp
  • A to G, chromosome 4 at 83,657,342 bp
  • A to T, chromosome 4 at 126,345,563 bp
  • T to A, chromosome 5 at 4,043,948 bp
  • T to C, chromosome 5 at 100,485,403 bp
  • T to C, chromosome 5 at 112,693,025 bp
  • T to C, chromosome 5 at 122,791,735 bp
  • G to A, chromosome 6 at 72,849,591 bp
  • A to G, chromosome 6 at 124,854,408 bp
  • A to G, chromosome 6 at 140,570,331 bp
  • T to A, chromosome 7 at 12,041,829 bp
  • G to T, chromosome 7 at 19,350,196 bp
  • A to T, chromosome 7 at 80,589,795 bp
  • T to A, chromosome 7 at 85,751,926 bp
  • T to C, chromosome 7 at 102,098,338 bp
  • T to C, chromosome 8 at 15,931,037 bp
  • A to T, chromosome 8 at 61,007,213 bp
  • A to G, chromosome 8 at 83,270,918 bp
  • T to A, chromosome 8 at 84,921,361 bp
  • A to G, chromosome 8 at 94,357,086 bp
  • A to C, chromosome 9 at 65,703,608 bp
  • A to G, chromosome 9 at 78,328,544 bp
  • A to G, chromosome 9 at 90,180,708 bp
  • C to A, chromosome 10 at 31,608,763 bp
  • T to C, chromosome 11 at 48,907,920 bp
  • T to C, chromosome 11 at 48,908,094 bp
  • C to T, chromosome 11 at 87,536,829 bp
  • T to G, chromosome 11 at 95,057,293 bp
  • C to T, chromosome 11 at 121,836,431 bp
  • A to T, chromosome 12 at 114,896,527 bp
  • T to A, chromosome 13 at 12,434,511 bp
  • T to C, chromosome 14 at 25,645,660 bp
  • T to C, chromosome 14 at 50,083,699 bp
  • T to A, chromosome 16 at 94,466,877 bp
  • T to C, chromosome 18 at 36,975,407 bp
  • T to C, chromosome 19 at 6,956,272 bp
  • G to A, chromosome X at 53,293,499 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4533 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041773-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.