Strain Name:
C57BL/6J-MtgxR4547Btlr/Mmmh
Stock Number:
041781-MU
Citation ID:
RRID:MMRRC_041781-MU
Other Names:
R4547 (G1), C57BL/6J-MtgxR4547Btlr
Major Collection:

Gene Information

Traf4
Name: TNF receptor associated factor 4
Synonyms: A530032M13Rik, CART1, msp2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 22032
Homologene: 3173
Cdh1
Name: cadherin 1
Synonyms: UM, E-cadherin, Ecad, L-CAM, uvomorulin, E-cad
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 12550
HGNC: HGNC:1748
Homologene: 20917
Smc2
Name: structural maintenance of chromosomes 2
Synonyms: Fin16, CAP-E, Smc2l1, 5730502P04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 14211
Homologene: 4705
Ankrd13a
Name: ankyrin repeat domain 13a
Synonyms: 1100001D10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 68420
Homologene: 23814
Dis3l2
Name: DIS3 like 3'-5' exoribonuclease 2
Synonyms: 4930429A22Rik, 8030493P09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 208718
Homologene: 62417
Aspm
Name: abnormal spindle microtubule assembly
Synonyms: Calmbp1, Aspm, Sha1, D330028K02Rik, MCPH5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 12316
Homologene: 7650
Arl6ip1
Name: ADP-ribosylation factor-like 6 interacting protein 1
Synonyms: ARMER, AIP-6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 54208
HGNC: HGNC:697
Homologene: 41008
Mettl25
Name: methyltransferase like 25
Synonyms: BC067068
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 216292
Homologene: 32774
Acin1
Name: apoptotic chromatin condensation inducer 1
Synonyms: Acinus, 2610510L13Rik, 2610036I19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 56215
Homologene: 22853
Ubn1
Name: ubinuclein 1
Synonyms: 1110029L11Rik, 2610108L02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 170644
VEGA: 16
Homologene: 9656
Trio
Name: triple functional domain (PTPRF interacting)
Synonyms: Solo, 6720464I07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 223435
VEGA: 15
Homologene: 20847
Fabp3
Name: fatty acid binding protein 3, muscle and heart
Synonyms: H-FABP, Mdgi, Fabp3, Fabph-1, Fabph4, Fabph1, Fabph-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 14077
HGNC: HGNC:3557
Homologene: 68379
Slc35f5
Name: solute carrier family 35, member F5
Synonyms: 1300003P13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 74150
Homologene: 5745
Slc44a4
Name: solute carrier family 44, member 4
Synonyms: NG22, 2210409B01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 70129
Homologene: 11359
Csmd1
Name: CUB and Sushi multiple domains 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 94109
Homologene: 69536
Tac1
Name: tachykinin 1
Synonyms: 4930528L02Rik, SP, PPT-A, neurokinin A, preprotachykinin A, NK1, neurokinin 1, substance P, NK-1, Nkna, PPTA
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 21333
Homologene: 2394
Zbed5
Name: zinc finger, BED type containing 5
Synonyms: 2410018M08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 71970
Homologene: 84838
Klb
Name: klotho beta
Synonyms: betaKlotho
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 83379
Homologene: 12820
Ano4
Name: anoctamin 4
Synonyms: A330096O15Rik, Tmem16d
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 320091
Homologene: 66599
Dnah6
Name: dynein, axonemal, heavy chain 6
Synonyms: A730004I20Rik, Dnahc6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 330355
HGNC: HGNC:2951
Homologene: 15221
Rnf213
Name: ring finger protein 213
Synonyms: D11Ertd759e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 672511
Homologene: 45439
Olfr720
Name: olfactory receptor 720
Synonyms: MOR274-2, GA_x6K02T2PLTE-6544896-6543946
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 258387
Homologene: 74036
Fat4
Name: FAT atypical cadherin 4
Synonyms: 6030410K14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 329628
Homologene: 14377
Ncdn
Name: neurochondrin
Synonyms: norbin, neurochondrin-1, neurochondrin-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 26562
Homologene: 8064
Tbc1d17
Name: TBC1 domain family, member 17
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233204
Homologene: 11656
Scara5
Name: scavenger receptor class A, member 5
Synonyms: 4932433F15Rik, 4933425F03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 71145
Homologene: 12556
Vsig8
Name: V-set and immunoglobulin domain containing 8
Synonyms: EG240916
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 240916
Homologene: 18616
Slc8a3
Name: solute carrier family 8 (sodium/calcium exchanger), member 3
Synonyms: Ncx3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 110893
Homologene: 62645
Kdm1b
Name: lysine (K)-specific demethylase 1B
Synonyms: 4632428N09Rik, Aof1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 218214
Homologene: 14695
Speer2
Name: spermatogenesis associated glutamate (E)-rich protein 2
Synonyms: SPEER-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 224318
Homologene: 69402
Frmd4a
Name: FERM domain containing 4A
Synonyms: Gm13190, 2700017I06Rik, C230040M21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 209630
Homologene: 9971
Gpr39
Name: G protein-coupled receptor 39
Synonyms: 4933415E13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 71111
HGNC: HGNC:4496
Homologene: 20380
Lnpk
Name: lunapark, ER junction formation factor
Synonyms: 9530051D01Rik, Lnpk1, 2310011O18Rik, lunapark, 4921514L11Rik, Lnp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 69605
Homologene: 12319
Synj1
Name: synaptojanin 1
Synonyms: A930006D20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 104015
Homologene: 48252
Dpm1
Name: dolichol-phosphate (beta-D) mannosyltransferase 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 13480
HGNC: HGNC:3005
Homologene: 2865
Naaa
Name: N-acylethanolamine acid amidase
Synonyms: Asahl, 3830414F09Rik, 2210023K21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 67111
HGNC: HGNC:736
Homologene: 8686
Mrc2
Name: mannose receptor, C type 2
Synonyms: novel lectin, Endo180, uPARAP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 17534
Homologene: 4408
Prss55
Name: protease, serine 55
Synonyms: 4933401F05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 71037
Homologene: 51233
Cfhr3
Name: complement factor H-related 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 624286
Homologene: 138664
Vmn2r60
Name: vomeronasal 2, receptor 60
Synonyms: Gprc2a-rs3, Casr-rs3, EG637898
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 637898
Homologene: 129683
Vmn1r19
Name: vomeronasal 1 receptor 19
Synonyms: V1rc27
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 171200
Homologene: 134034
Nlrp4e
Name: NLR family, pyrin domain containing 4E
Synonyms: 4930406H16Rik, Nalp-epsilon, Nalp4e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 446099
Homologene: 75315
Tmem132a
Name: transmembrane protein 132A
Synonyms: Hspa5bp1, 6720481D13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 98170
Homologene: 75076
Olfr1122
Name: olfactory receptor 1122
Synonyms: GA_x6K02T2Q125-48880078-48881058, MOR264-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 259033
Homologene: 73977
Hace1
Name: HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1
Synonyms: A730034A22Rik, 1700042J16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 209462
Homologene: 10807
Psg25
Name: pregnancy-specific glycoprotein 25
Synonyms: cea13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 114868
Homologene: 110989
Mrm2
Name: mitochondrial rRNA methyltransferase 2
Synonyms: Ftsj2, 2310037B18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 68017
Homologene: 5907
Nupl2
Name: nucleoporin like 2
Synonyms: CG1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231042
Homologene: 40573
Gm15841
Name: predicted gene 15841
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 74,907,612 bp
  • A to T, chromosome 1 at 87,049,671 bp
  • T to C, chromosome 1 at 125,572,382 bp
  • G to A, chromosome 1 at 125,677,991 bp
  • T to C, chromosome 1 at 139,478,187 bp
  • T to G, chromosome 1 at 139,584,913 bp
  • A to T, chromosome 1 at 172,560,596 bp
  • C to A, chromosome 2 at 4,473,145 bp
  • C to G, chromosome 2 at 74,522,286 bp
  • G to A, chromosome 2 at 87,388,160 bp
  • A to G, chromosome 2 at 168,223,153 bp
  • T to C, chromosome 3 at 38,951,283 bp
  • T to C, chromosome 4 at 52,467,866 bp
  • A to G, chromosome 4 at 126,746,674 bp
  • C to T, chromosome 4 at 130,312,452 bp
  • T to C, chromosome 5 at 24,177,970 bp
  • T to A, chromosome 5 at 65,379,928 bp
  • C to T, chromosome 5 at 92,263,586 bp
  • A to G, chromosome 5 at 114,775,296 bp
  • T to A, chromosome 5 at 129,902,851 bp
  • T to C, chromosome 5 at 140,328,496 bp
  • A to G, chromosome 6 at 7,557,216 bp
  • C to T, chromosome 6 at 57,404,789 bp
  • A to T, chromosome 6 at 73,192,405 bp
  • A to G, chromosome 7 at 18,524,704 bp
  • A to G, chromosome 7 at 23,336,866 bp
  • A to G, chromosome 7 at 42,135,663 bp
  • A to G, chromosome 7 at 44,841,347 bp
  • AAAATAAATAAATAAATAAATAAATA to AAAATAAATAAATAAATAAATAAATAAATA, chromosome 7 at 118,121,899 bp
  • T to C, chromosome 8 at 16,391,797 bp
  • C to A, chromosome 8 at 106,663,903 bp
  • T to A, chromosome 10 at 45,672,555 bp
  • T to C, chromosome 10 at 88,981,170 bp
  • T to C, chromosome 10 at 105,826,017 bp
  • A to G, chromosome 11 at 78,161,037 bp
  • G to A, chromosome 11 at 105,336,641 bp
  • G to T, chromosome 11 at 119,479,670 bp
  • A to G, chromosome 12 at 81,314,851 bp
  • C to T, chromosome 13 at 47,063,077 bp
  • A to T, chromosome 14 at 14,175,854 bp
  • G to T, chromosome 14 at 54,645,667 bp
  • T to C, chromosome 14 at 64,079,428 bp
  • A to C, chromosome 14 at 65,670,574 bp
  • T to C, chromosome 15 at 27,818,982 bp
  • G to A, chromosome 16 at 5,072,092 bp
  • T to C, chromosome 16 at 69,858,849 bp
  • T to C, chromosome 16 at 90,988,282 bp
  • T to A, chromosome 17 at 34,927,755 bp
  • A to G, chromosome 19 at 10,860,200 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4547 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041781-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.