Strain Name:
C57BL/6J-MtgxR4689Btlr/Mmmh
Stock Number:
041940-MU
Citation ID:
RRID:MMRRC_041940-MU
Other Names:
R4689 (G1), C57BL/6J-MtgxR4689Btlr
Major Collection:

Gene Information

Nos1
Name: nitric oxide synthase 1, neuronal
Synonyms: bNOS, nNOS, NO, Nos-1, 2310005C01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 18125
HGNC: HGNC:7872
Homologene: 37327
Mbd5
Name: methyl-CpG binding domain protein 5
Synonyms: OTTMUSG00000012483, 9430004D19Rik, C030040A15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 109241
Homologene: 81861
Frem2
Name: Fras1 related extracellular matrix protein 2
Synonyms: b2b1562Clo, my, 8430406N05Rik, 6030440P17Rik, ne
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 242022
Homologene: 18454
Rgs11
Name: regulator of G-protein signaling 11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 50782
HGNC: HGNC:9993
Homologene: 77719
Rpl3
Name: ribosomal protein L3
Synonyms: F2, J1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 27367
Homologene: 747
Nmral1
Name: NmrA-like family domain containing 1
Synonyms: 1110025F24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 67824
Homologene: 41388
Kif13b
Name: kinesin family member 13B
Synonyms: C130021D12Rik, 5330429L19Rik, N-3 kinesin, GAKIN
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 16554
VEGA: 14
Homologene: 9073
Larp1
Name: La ribonucleoprotein domain family, member 1
Synonyms: Larp, 3110040D16Rik, 1810024J12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 73158
Homologene: 9089
Prpf3
Name: pre-mRNA processing factor 3
Synonyms: 3632413F13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 70767
Homologene: 3447
Lfng
Name: LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase
Synonyms: lunatic fringe
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 16848
HGNC: HGNC:6560
Homologene: 22475
Cdca8
Name: cell division cycle associated 8
Synonyms: DasraB, D4Ertd421e, 4831429J16Rik, Borealin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 52276
Homologene: 23075
Zfp318
Name: zinc finger protein 318
Synonyms: TZF, D530032D06Rik, 2610034E08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 57908
Homologene: 22808
Hoxa3
Name: homeobox A3
Synonyms: Mo-10, Hox-1.5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 15400
HGNC: HGNC:5104
Homologene: 40725
Rasa1
Name: RAS p21 protein activator 1
Synonyms: p120-rasGAP, Gap
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 218397
HGNC: HGNC:9871
Homologene: 2168
Prkaa1
Name: protein kinase, AMP-activated, alpha 1 catalytic subunit
Synonyms: C130083N04Rik, AMPKalpha1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 105787
VEGA: 15
HGNC: HGNC:9376
Homologene: 49590
Ahcyl1
Name: S-adenosylhomocysteine hydrolase-like 1
Synonyms: 1110034F20Rik, IRBIT, DCAL, Ahcy-rs3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229709
HGNC: HGNC:344
Homologene: 77353
Flcn
Name: folliculin
Synonyms: B430214A04Rik, BHD
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 216805
Homologene: 14583
Hydin
Name: HYDIN, axonemal central pair apparatus protein
Synonyms: hy3, hy-3, 4930545D19Rik, hyrh, 1700034M11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 244653
Homologene: 52118
Myo16
Name: myosin XVI
Synonyms: BM140241, C230040D10Rik, Nyap3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 244281
Homologene: 34710
F5
Name: coagulation factor V
Synonyms: Cf5, Cf-5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 14067
HGNC: HGNC:3542
Homologene: 104
Dnah12
Name: dynein, axonemal, heavy chain 12
Synonyms: Hdhc3, Dnahc7l, LOC380889, HL-19, DLP12, HL19, DHC3, 4921531P07Rik, Dnahc12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 110083
HGNC: HGNC:2943
Homologene: 56821
Adgrb3
Name: adhesion G protein-coupled receptor B3
Synonyms: A830096D10Rik, Bai3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 210933
HGNC: HGNC:945
Homologene: 1289
Naip2
Name: NLR family, apoptosis inhibitory protein 2
Synonyms: Naip2, Naip-rs6, Birc1b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 17948
HGNC: HGNC:7634
Homologene: 136092
Wdfy4
Name: WD repeat and FYVE domain containing 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 545030
Homologene: 83473
Myh7b
Name: myosin, heavy chain 7B, cardiac muscle, beta
Synonyms: Myh14
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 668940
Homologene: 66117
Slc45a1
Name: solute carrier family 45, member 1
Synonyms: Dnb5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 242773
Homologene: 44908
Ptprh
Name: protein tyrosine phosphatase, receptor type, H
Synonyms: SAP-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 545902
HGNC: HGNC:9672
Homologene: 37693
Olfr800
Name: olfactory receptor 800
Synonyms: MOR114-7, GA_x6K02T2PULF-11338429-11339364
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 258541
Homologene: 27203
Vmn2r1
Name: vomeronasal 2, receptor 1
Synonyms: EG56544, V2r83
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 56544
Homologene: 84832
Krtap9-5
Name: keratin associated protein 9-5
Synonyms: OTTMUSG00000002205
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 435286
Homologene: 135766
Olfr169
Name: olfactory receptor 169
Synonyms: GA_x54KRFPKG5P-16014972-16014031, MOR273-3P
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 258158
Homologene: 128058
Adgre4
Name: adhesion G protein-coupled receptor E4
Synonyms: FIRE, EGF-TM7, Gpr127, D17Ertd479e, Emr4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 52614
VEGA: 17
Homologene: 64640
Shank2
Name: SH3 and multiple ankyrin repeat domains 2
Synonyms: ProSAP1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 210274
Homologene: 105965
Fmnl1
Name: formin-like 1
Synonyms: 8030453N10Rik, formin-related gene in leukocytes
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 57778
HGNC: HGNC:1212
Homologene: 135620
Trp63
Name: transformation related protein 63
Synonyms: p73L, p63, TAp63, KET protein, Trp53rp1, deltaNp63, p51/p63
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 22061
Homologene: 31189
Acbd4
Name: acyl-Coenzyme A binding domain containing 4
Synonyms: 2010015A21Rik, 2010009P05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 67131
Homologene: 32596
Dthd1
Name: death domain containing 1
Synonyms: Gm17384
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 100322896
Homologene: 123510
Gfra3
Name: glial cell line derived neurotrophic factor family receptor alpha 3
Synonyms: GFRalpha3, GFR alpha-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 14587
VEGA: 18
HGNC: HGNC:4245
Homologene: 1146
Adam10
Name: a disintegrin and metallopeptidase domain 10
Synonyms: kuzbanian, 1700031C13Rik, kuz
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 11487
HGNC: HGNC:188
Homologene: 865
Nrap
Name: nebulin-related anchoring protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 18175
HGNC: HGNC:7988
Homologene: 4499
Vmn1r235
Name: vomeronasal 1 receptor 235
Synonyms: V1rf2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 171233
Homologene: 74360
Zfp661
Name: zinc finger protein 661
Synonyms: 2810405K07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 72180
Homologene: 23275
Zfp955a
Name: zinc finger protein 955A
Synonyms: AI842447
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 77652
VEGA: 17
Homologene: 104925
Dubr
Name: Dppa2 upstream binding RNA
Synonyms: Dum, 5330426P16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 68190
Slc39a10
Name: solute carrier family 39 (zinc transporter), member 10
Synonyms: 2900042E17Rik, Zip10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 227059
Homologene: 34076
Gzmd
Name: granzyme D
Synonyms: CCP5, Ctla-5, Ctla5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 14941
HGNC: HGNC:4710
Homologene: 133275
Olfr1509
Name: olfactory receptor 1509
Synonyms: GA_x6K02T2RJGY-534312-533386, MOR83, MOR244-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 57271
HGNC: HGNC:8297
Homologene: 41378
Tec
Name: tec protein tyrosine kinase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 21682
Homologene: 1302
Hexb
Name: hexosaminidase B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 15212
VEGA: 13
HGNC: HGNC:4879
Homologene: 437
Stt3a
Name: STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae)
Synonyms: Itm1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 16430
HGNC: HGNC:6172
Homologene: 40617
Pkdcc
Name: protein kinase domain containing, cytoplasmic
Synonyms: MAd1, ESTM17, Adtk1, Vlk
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 106522
Homologene: 18932
Zfp937
Name: zinc finger protein 937
Synonyms: Gm4979
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 245174
Homologene: 136215
Abcc10
Name: ATP-binding cassette, sub-family C (CFTR/MRP), member 10
Synonyms: Mrp7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 224814
HGNC: HGNC:52
Homologene: 58616
Plekhg6
Name: pleckstrin homology domain containing, family G (with RhoGef domain) member 6
Synonyms: LOC213522
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 213522
Homologene: 32395
Slc40a1
Name: solute carrier family 40 (iron-regulated transporter), member 1
Synonyms: Ol5, metal transporting protein 1, Dusg, Slc11a3, Pcm, IREG1, MTP1, ferroportin1, FPN1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 53945
Homologene: 40959
Aldh3b3
Name: aldehyde dehydrogenase 3 family, member B3
Synonyms: 1700055N04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 73458
HGNC: HGNC:411
Homologene: 133295
Mterf1b
Name: mitochondrial transcription termination factor 1b
Synonyms: Gm9897
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 208595
Homologene: 5073
Fstl4
Name: follistatin-like 4
Synonyms: B230374F23Rik, SPIG1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 320027
Homologene: 18543
Dkk4
Name: dickkopf WNT signaling pathway inhibitor 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234130
HGNC: HGNC:2894
Homologene: 8680
Cyp2c67
Name: cytochrome P450, family 2, subfamily c, polypeptide 67
Synonyms: C730004C24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 545288
Homologene: 74936
Cry2
Name: cryptochrome 2 (photolyase-like)
Synonyms: D130054K12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 12953
HGNC: HGNC:2385
Homologene: 56466
Serpinb13
Name: serine (or cysteine) peptidase inhibitor, clade B (ovalbumin), member 13
Synonyms: headpin, HUR7, PI13, HURPIN
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 241196
HGNC: HGNC:8944
Homologene: 22718
Ifi213
Name: interferon activated gene 213
Synonyms: E030037K03Rik, Pydc4, Pyr-A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 623121
HGNC: HGNC:5395
Homologene: 115929
Zfp358
Name: zinc finger protein 358
Synonyms: Zfend
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 140482
Homologene: 9996
Stambpl1
Name: STAM binding protein like 1
Synonyms: 8230401J17Rik, 1700095N21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 76630
Homologene: 23253
Zcchc10
Name: zinc finger, CCHC domain containing 10
Synonyms: D11Ertd416e, 2410141K03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 67966
Psma5-ps
Name: proteasome subunit alpha 5, pseudogene
Synonyms: Gm8394
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 666974
VEGA: 10
Rab36
Name: RAB36, member RAS oncogene family
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 76877
HGNC: HGNC:9775
Homologene: 3610
Gm8730
Name: predicted pseudogene 8730
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 667618
Olfr106-ps
Name: olfactory receptor 106, pseudogene
Synonyms: GA_x6K02T2PSCP-1855511-1856437, Olfr106, MOR250-6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 257925
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 25,559,810 bp
  • T to A, chromosome 1 at 45,912,313 bp
  • A to C, chromosome 1 at 46,836,013 bp
  • C to T, chromosome 1 at 106,982,844 bp
  • T to C, chromosome 1 at 164,151,973 bp
  • G to A, chromosome 1 at 173,590,420 bp
  • G to A, chromosome 2 at 49,258,279 bp
  • T to C, chromosome 2 at 92,424,554 bp
  • G to A, chromosome 2 at 127,577,548 bp
  • T to C, chromosome 2 at 150,236,786 bp
  • T to C, chromosome 2 at 155,630,514 bp
  • A to T, chromosome 3 at 53,547,635 bp
  • A to G, chromosome 3 at 64,104,653 bp
  • G to A, chromosome 3 at 95,836,489 bp
  • G to T, chromosome 3 at 107,665,518 bp
  • C to T, chromosome 4 at 124,931,103 bp
  • A to T, chromosome 4 at 150,638,539 bp
  • T to A, chromosome 5 at 4,197,263 bp
  • T to C, chromosome 5 at 62,842,912 bp
  • G to A, chromosome 5 at 72,823,637 bp
  • A to G, chromosome 5 at 117,879,385 bp
  • A to G, chromosome 5 at 140,614,439 bp
  • C to A, chromosome 6 at 52,213,311 bp
  • G to A, chromosome 6 at 125,373,181 bp
  • T to A, chromosome 7 at 4,597,997 bp
  • G to A, chromosome 7 at 144,420,605 bp
  • T to C, chromosome 8 at 3,496,146 bp
  • T to C, chromosome 8 at 10,438,890 bp
  • T to C, chromosome 8 at 22,625,320 bp
  • T to A, chromosome 8 at 102,865,747 bp
  • A to T, chromosome 8 at 110,595,414 bp
  • T to C, chromosome 9 at 36,732,929 bp
  • T to C, chromosome 9 at 70,765,954 bp
  • T to C, chromosome 10 at 75,041,933 bp
  • T to A, chromosome 10 at 85,314,201 bp
  • T to C, chromosome 10 at 129,660,316 bp
  • C to T, chromosome 11 at 53,068,650 bp
  • A to G, chromosome 11 at 53,327,324 bp
  • G to T, chromosome 11 at 58,041,613 bp
  • A to C, chromosome 11 at 59,801,044 bp
  • G to T, chromosome 11 at 99,949,460 bp
  • T to C, chromosome 11 at 103,105,368 bp
  • G to A, chromosome 11 at 103,193,736 bp
  • A to T, chromosome 13 at 85,238,163 bp
  • A to G, chromosome 13 at 97,181,092 bp
  • A to T, chromosome 13 at 100,148,812 bp
  • G to A, chromosome 14 at 26,706,839 bp
  • A to G, chromosome 14 at 33,109,548 bp
  • T to C, chromosome 14 at 52,451,214 bp
  • T to C, chromosome 14 at 56,131,226 bp
  • T to A, chromosome 14 at 64,773,064 bp
  • A to G, chromosome 15 at 5,178,696 bp
  • G to A, chromosome 15 at 80,080,391 bp
  • A to G, chromosome 16 at 4,714,558 bp
  • A to T, chromosome 16 at 19,566,513 bp
  • A to T, chromosome 16 at 25,865,262 bp
  • A to T, chromosome 16 at 50,732,503 bp
  • A to G, chromosome 17 at 21,262,361 bp
  • T to C, chromosome 17 at 26,204,547 bp
  • T to C, chromosome 17 at 33,242,066 bp
  • A to T, chromosome 17 at 37,394,771 bp
  • C to A, chromosome 17 at 46,324,070 bp
  • T to A, chromosome 17 at 46,399,634 bp
  • T to C, chromosome 17 at 55,802,096 bp
  • C to G, chromosome 17 at 83,215,861 bp
  • G to A, chromosome 18 at 34,690,587 bp
  • G to A, chromosome 19 at 3,964,516 bp
  • A to G, chromosome 19 at 34,236,291 bp
  • T to C, chromosome 19 at 39,638,588 bp
  • A to G, chromosome 19 at 56,386,026 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4689 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041940-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.