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Strain Name:
C57BL/6J-MtgxR4720Btlr/Mmmh
Stock Number:
041958-MU
Citation ID:
RRID:MMRRC_041958-MU
Other Names:
R4720 (G1), C57BL/6J-MtgxR4720Btlr
Major Collection:

Strain Information

Map2k5
Name: mitogen-activated protein kinase kinase 5
Synonyms: MEK5
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 23938
VEGA: 9
HGNC: HGNC:6845
Homologene: 115933
Htr2a
Name: 5-hydroxytryptamine (serotonin) receptor 2A
Synonyms: 5-HT2A receptor, Htr-2, Htr2
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 15558
VEGA: 14
HGNC: HGNC:5293
Homologene: 68073
Ece1
Name: endothelin converting enzyme 1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230857
HGNC: HGNC:3146
Homologene: 1068
Tjp2
Name: tight junction protein 2
Synonyms: ZO-2
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 21873
VEGA: 19
Homologene: 3541
Dnajc11
Name: DnaJ heat shock protein family (Hsp40) member C11
Synonyms: E030019A03Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230935
Homologene: 14558
Crybg3
Name: beta-gamma crystallin domain containing 3
Synonyms: Gm9581
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 224273
Homologene: 28544
Lrp2
Name: low density lipoprotein receptor-related protein 2
Synonyms: Megalin, Gp330, D230004K18Rik, b2b1625.2Clo
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 14725
HGNC: HGNC:6694
Homologene: 20952
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 21,403,050 bp
  • A to G, chromosome 1 at 22,427,481 bp
  • A to G, chromosome 1 at 40,446,678 bp
  • C to T, chromosome 1 at 45,822,485 bp
  • T to C, chromosome 1 at 58,678,348 bp
  • A to G, chromosome 1 at 64,713,131 bp
  • T to A, chromosome 1 at 66,510,792 bp
  • A to T, chromosome 1 at 72,699,011 bp
  • A to T, chromosome 1 at 90,941,697 bp
  • T to C, chromosome 1 at 105,750,984 bp
  • A to T, chromosome 1 at 110,895,381 bp
  • T to C, chromosome 1 at 152,756,674 bp
  • A to G, chromosome 1 at 181,101,587 bp
  • T to C, chromosome 1 at 191,763,667 bp
  • T to A, chromosome 2 at 36,812,472 bp
  • C to T, chromosome 2 at 53,107,540 bp
  • T to G, chromosome 2 at 69,481,173 bp
  • T to G, chromosome 2 at 71,233,674 bp
  • T to C, chromosome 2 at 106,783,746 bp
  • A to G, chromosome 2 at 135,251,747 bp
  • T to G, chromosome 2 at 178,374,432 bp
  • C to T, chromosome 2 at 180,027,981 bp
  • T to C, chromosome 2 at 181,238,417 bp
  • A to G, chromosome 3 at 68,962,290 bp
  • G to T, chromosome 3 at 93,447,882 bp
  • C to A, chromosome 3 at 103,929,020 bp
  • T to C, chromosome 4 at 58,205,869 bp
  • T to C, chromosome 4 at 108,644,368 bp
  • T to C, chromosome 4 at 117,171,749 bp
  • T to C, chromosome 4 at 127,195,715 bp
  • G to A, chromosome 4 at 137,957,175 bp
  • T to A, chromosome 4 at 150,866,747 bp
  • A to G, chromosome 4 at 151,968,539 bp
  • A to T, chromosome 5 at 22,286,896 bp
  • T to C, chromosome 5 at 36,484,875 bp
  • A to G, chromosome 5 at 114,229,914 bp
  • T to C, chromosome 5 at 123,765,023 bp
  • GCGGATGCGCAC to GC, chromosome 5 at 123,864,689 bp
  • T to G, chromosome 6 at 39,640,407 bp
  • A to T, chromosome 6 at 67,423,661 bp
  • C to T, chromosome 6 at 87,383,525 bp
  • C to T, chromosome 6 at 88,911,764 bp
  • T to C, chromosome 6 at 102,242,022 bp
  • T to C, chromosome 6 at 116,288,161 bp
  • T to A, chromosome 6 at 124,832,164 bp
  • C to A, chromosome 6 at 141,723,222 bp
  • C to T, chromosome 7 at 9,023,470 bp
  • C to A, chromosome 7 at 38,099,347 bp
  • T to A, chromosome 7 at 44,374,336 bp
  • T to A, chromosome 7 at 140,037,511 bp
  • T to C, chromosome 8 at 74,873,859 bp
  • T to A, chromosome 9 at 38,516,393 bp
  • A to G, chromosome 9 at 63,293,719 bp
  • T to A, chromosome 10 at 25,471,626 bp
  • G to A, chromosome 10 at 45,314,906 bp
  • A to G, chromosome 10 at 75,509,660 bp
  • A to G, chromosome 11 at 43,203,122 bp
  • A to T, chromosome 11 at 53,988,893 bp
  • T to C, chromosome 11 at 66,076,358 bp
  • G to C, chromosome 11 at 89,441,426 bp
  • A to T, chromosome 11 at 96,827,689 bp
  • A to T, chromosome 11 at 99,780,445 bp
  • A to G, chromosome 11 at 110,127,422 bp
  • A to C, chromosome 11 at 118,398,469 bp
  • A to C, chromosome 12 at 69,324,288 bp
  • A to G, chromosome 12 at 76,404,865 bp
  • G to T, chromosome 12 at 76,578,322 bp
  • GTCAAA to G, chromosome 12 at 79,075,420 bp
  • C to A, chromosome 12 at 98,979,809 bp
  • A to G, chromosome 12 at 104,130,630 bp
  • T to C, chromosome 13 at 49,662,753 bp
  • T to A, chromosome 13 at 55,003,883 bp
  • G to A, chromosome 13 at 55,543,437 bp
  • G to A, chromosome 13 at 69,623,232 bp
  • C to T, chromosome 13 at 81,669,465 bp
  • T to A, chromosome 14 at 51,413,245 bp
  • T to A, chromosome 14 at 74,645,059 bp
  • A to G, chromosome 15 at 38,493,500 bp
  • G to A, chromosome 15 at 80,560,309 bp
  • A to C, chromosome 15 at 98,815,939 bp
  • C to A, chromosome 15 at 101,135,773 bp
  • T to A, chromosome 16 at 15,667,715 bp
  • T to C, chromosome 16 at 36,925,874 bp
  • A to G, chromosome 16 at 59,539,817 bp
  • G to T, chromosome 16 at 62,822,319 bp
  • C to A, chromosome 17 at 19,522,526 bp
  • C to T, chromosome 17 at 30,683,634 bp
  • T to G, chromosome 17 at 45,556,306 bp
  • G to A, chromosome 18 at 4,674,055 bp
  • A to T, chromosome 18 at 45,683,120 bp
  • G to T, chromosome 18 at 60,270,063 bp
  • G to A, chromosome 18 at 60,564,391 bp
  • G to T, chromosome 18 at 76,861,183 bp
  • G to A, chromosome 19 at 3,886,375 bp
  • T to C, chromosome 19 at 6,857,715 bp
  • T to C, chromosome 19 at 10,648,467 bp
  • T to C, chromosome 19 at 24,100,805 bp
  • C to A, chromosome 19 at 34,021,699 bp
  • C to A, chromosome 19 at 38,789,622 bp
  • G to C, chromosome 19 at 41,585,895 bp
  • A to T, chromosome 19 at 60,870,113 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4720 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041958-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.