Strain Name:
C57BL/6J-MtgxR4883Btlr/Mmmh
Stock Number:
042491-MU
Citation ID:
RRID:MMRRC_042491-MU
Other Names:
R4883 (G1), C57BL/6J-MtgxR4883Btlr
Major Collection:

Gene Information

Gene Symbol: Btrc [MGI:1338871] (Mus musculus (mouse))
Name: beta-transducin repeat containing protein
Synonyms: beta-TrCP, SCF b-TRCP, Slimb, Fbw1a, Beta-Trcp1
Chromosome: 19
Alteration at locus: Chemically Induced
Gene Symbol: Nedd4 [MGI:97297] (Mus musculus (mouse))
Name: neural precursor cell expressed, developmentally down-regulated 4
Synonyms: Nedd4-1, Nedd4a, E430025J12Rik, Nedd4
Chromosome: 9
Alteration at locus: Chemically Induced
Gene Symbol: Zfa-ps [MGI:99153] (Mus musculus (mouse))
Name: zinc finger protein, autosomal, pseudogene
Synonyms: Zfa
Chromosome: 10
Alteration at locus: Chemically Induced
Gene Symbol: Ngf [MGI:97321] (Mus musculus (mouse))
Name: nerve growth factor
Synonyms: Ngfb
Chromosome: 3
Alteration at locus: Chemically Induced
Gene Symbol: Odc1 [MGI:97402] (Mus musculus (mouse))
Name: ornithine decarboxylase, structural 1
Synonyms: ODC
Chromosome: 12
Alteration at locus: Chemically Induced
Gene Symbol: Pcdh9 [MGI:1306801] (Mus musculus (mouse))
Name: protocadherin 9
Synonyms: C530050I23Rik, LOC382930, C630029H24Rik, 1500001L12Rik, A730003J17Rik
Chromosome: 14
Alteration at locus: Chemically Induced
Gene Symbol: Pomt1 [MGI:2138994] (Mus musculus (mouse))
Name: protein-O-mannosyltransferase 1
Chromosome: 2
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 9,747,198 bp
  • A to G, chromosome 1 at 36,552,016 bp
  • A to T, chromosome 1 at 107,540,951 bp
  • C to G, chromosome 1 at 133,384,428 bp
  • A to T, chromosome 1 at 135,847,758 bp
  • T to C, chromosome 1 at 180,118,728 bp
  • G to T, chromosome 1 at 181,001,923 bp
  • C to T, chromosome 1 at 189,850,800 bp
  • C to A, chromosome 2 at 32,254,458 bp
  • C to T, chromosome 2 at 89,482,308 bp
  • A to T, chromosome 2 at 120,512,816 bp
  • G to A, chromosome 2 at 155,406,767 bp
  • C to T, chromosome 3 at 37,642,719 bp
  • G to A, chromosome 3 at 87,259,615 bp
  • T to A, chromosome 3 at 88,107,535 bp
  • T to A, chromosome 3 at 102,520,645 bp
  • T to A, chromosome 4 at 43,665,414 bp
  • A to T, chromosome 4 at 115,833,731 bp
  • T to A, chromosome 4 at 136,290,481 bp
  • T to A, chromosome 4 at 137,000,930 bp
  • T to A, chromosome 4 at 137,328,804 bp
  • G to A, chromosome 4 at 150,616,053 bp
  • A to T, chromosome 5 at 14,676,139 bp
  • A to T, chromosome 5 at 74,607,869 bp
  • T to A, chromosome 5 at 81,689,646 bp
  • A to G, chromosome 5 at 91,593,916 bp
  • T to G, chromosome 5 at 92,951,134 bp
  • A to G, chromosome 5 at 95,340,454 bp
  • A to T, chromosome 5 at 99,233,995 bp
  • C to T, chromosome 5 at 106,983,358 bp
  • T to A, chromosome 5 at 122,681,066 bp
  • T to C, chromosome 5 at 124,299,045 bp
  • T to A, chromosome 5 at 128,269,300 bp
  • T to A, chromosome 5 at 128,269,302 bp
  • A to G, chromosome 5 at 130,013,961 bp
  • T to A, chromosome 5 at 146,493,926 bp
  • T to A, chromosome 6 at 3,714,705 bp
  • A to T, chromosome 6 at 24,134,357 bp
  • A to T, chromosome 6 at 37,558,401 bp
  • A to T, chromosome 6 at 48,460,822 bp
  • A to G, chromosome 7 at 5,196,444 bp
  • A to G, chromosome 7 at 15,556,338 bp
  • G to T, chromosome 7 at 30,355,813 bp
  • A to G, chromosome 7 at 38,400,365 bp
  • A to T, chromosome 7 at 45,159,668 bp
  • A to G, chromosome 7 at 45,644,414 bp
  • A to T, chromosome 7 at 59,243,450 bp
  • A to C, chromosome 7 at 103,716,707 bp
  • A to T, chromosome 7 at 141,212,618 bp
  • A to G, chromosome 8 at 93,224,088 bp
  • A to G, chromosome 8 at 105,279,256 bp
  • G to T, chromosome 8 at 117,607,133 bp
  • G to A, chromosome 8 at 124,741,084 bp
  • T to C, chromosome 9 at 7,355,512 bp
  • G to T, chromosome 9 at 44,279,319 bp
  • C to T, chromosome 9 at 49,541,883 bp
  • A to G, chromosome 9 at 57,146,922 bp
  • G to A, chromosome 9 at 72,740,233 bp
  • G to T, chromosome 9 at 86,569,325 bp
  • A to T, chromosome 9 at 103,499,077 bp
  • C to T, chromosome 10 at 4,007,775 bp
  • A to T, chromosome 10 at 28,588,932 bp
  • A to T, chromosome 10 at 29,196,541 bp
  • G to T, chromosome 10 at 33,949,042 bp
  • A to T, chromosome 10 at 52,545,035 bp
  • A to G, chromosome 10 at 58,561,996 bp
  • T to A, chromosome 10 at 63,285,654 bp
  • G to T, chromosome 10 at 94,139,853 bp
  • T to C, chromosome 10 at 96,617,397 bp
  • T to A, chromosome 10 at 115,410,642 bp
  • T to C, chromosome 10 at 127,182,933 bp
  • T to C, chromosome 11 at 4,146,566 bp
  • C to T, chromosome 11 at 59,002,958 bp
  • T to C, chromosome 11 at 62,780,629 bp
  • T to C, chromosome 11 at 72,214,149 bp
  • T to A, chromosome 11 at 73,069,752 bp
  • T to A, chromosome 11 at 84,251,290 bp
  • T to C, chromosome 11 at 98,629,567 bp
  • G to A, chromosome 11 at 101,634,825 bp
  • T to C, chromosome 11 at 110,326,631 bp
  • T to A, chromosome 12 at 17,547,385 bp
  • A to T, chromosome 12 at 51,784,247 bp
  • A to G, chromosome 12 at 81,315,672 bp
  • A to G, chromosome 12 at 103,094,333 bp
  • G to T, chromosome 12 at 104,782,048 bp
  • A to G, chromosome 12 at 113,327,518 bp
  • T to A, chromosome 12 at 118,849,070 bp
  • C to T, chromosome 13 at 21,555,488 bp
  • A to G, chromosome 13 at 55,010,922 bp
  • C to A, chromosome 13 at 63,317,179 bp
  • G to A, chromosome 13 at 73,267,631 bp
  • T to A, chromosome 13 at 117,902,895 bp
  • T to A, chromosome 14 at 34,335,390 bp
  • T to C, chromosome 14 at 42,311,426 bp
  • C to T, chromosome 14 at 47,551,314 bp
  • T to G, chromosome 14 at 49,052,364 bp
  • T to A, chromosome 14 at 49,245,103 bp
  • T to C, chromosome 14 at 68,638,103 bp
  • T to A, chromosome 14 at 93,888,728 bp
  • A to G, chromosome 15 at 7,185,625 bp
  • A to T, chromosome 15 at 28,343,638 bp
  • A to T, chromosome 15 at 55,642,802 bp
  • T to C, chromosome 15 at 63,835,765 bp
  • A to G, chromosome 15 at 86,496,916 bp
  • A to G, chromosome 16 at 17,277,635 bp
  • A to T, chromosome 16 at 32,256,978 bp
  • A to T, chromosome 16 at 44,219,579 bp
  • A to G, chromosome 16 at 93,870,362 bp
  • T to C, chromosome 17 at 15,403,032 bp
  • T to C, chromosome 17 at 24,982,319 bp
  • T to A, chromosome 17 at 29,282,192 bp
  • T to C, chromosome 17 at 38,130,617 bp
  • G to T, chromosome 17 at 43,615,833 bp
  • T to C, chromosome 17 at 56,783,747 bp
  • C to A, chromosome 17 at 85,613,685 bp
  • A to G, chromosome 19 at 34,966,122 bp
  • C to T, chromosome 19 at 45,456,587 bp
  • C to T, chromosome 19 at 50,232,303 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4883 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
042491-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.