Strain Name:
C57BL/6J-MtgxR4912Btlr/Mmmh
Stock Number:
042514-MU
Citation ID:
RRID:MMRRC_042514-MU
Other Names:
R4912 (G1), C57BL/6J-MtgxR4912Btlr
Major Collection:

Strain Information

Mon1b
Name: MON1 homolog B, secretory traffciking associated
Synonyms: 5033413H12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 270096
Homologene: 69174
Utp20
Name: UTP20 small subunit processome component
Synonyms: mDRIM, DRIM, 3830408P06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 70683
VEGA: 10
Homologene: 38373
Sipa1l1
Name: signal-induced proliferation-associated 1 like 1
Synonyms: 4931426N11Rik, Spar
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 217692
VEGA: 12
Homologene: 9189
Maco1
Name: macoilin 1
Synonyms: 9230118A01Rik, C61, 1110007C24Rik, Tmem57
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 66146
Homologene: 14449
Arhgef12
Name: Rho guanine nucleotide exchange factor (GEF) 12
Synonyms: LARG, 2310014B11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 69632
VEGA: 9
Homologene: 9088
Fryl
Name: FRY like transcription coactivator
Synonyms: 2310004H21Rik, 2510002A14Rik, 9030227G01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 72313
Homologene: 103956
Ulk1
Name: unc-51 like kinase 1
Synonyms: Unc51.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 22241
Homologene: 2640
Zranb3
Name: zinc finger, RAN-binding domain containing 3
Synonyms: 4933425L19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 226409
Homologene: 32770
Ltbp4
Name: latent transforming growth factor beta binding protein 4
Synonyms: 2310046A13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 108075
HGNC: HGNC:6717
Homologene: 2645
Usp5
Name: ubiquitin specific peptidase 5 (isopeptidase T)
Synonyms: Ucht
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 22225
Homologene: 55758
Cpne3
Name: copine III
Synonyms: PRO1071, CPN3, 5730450C07Rik, 5430428M23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 70568
HGNC: HGNC:2316
Homologene: 20839
Usp30
Name: ubiquitin specific peptidase 30
Synonyms: 6330590F17Rik, D5Ertd483e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 100756
Homologene: 32789
Diaph3
Name: diaphanous related formin 3
Synonyms: p134MDia2, mDia2, 4930417P13Rik, Drf3, Diap3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 56419
VEGA: 14
Homologene: 84829
Rexo4
Name: REX4, 3'-5' exonuclease
Synonyms: XPMC2H, Rex4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 227656
Homologene: 133948
Birc6
Name: baculoviral IAP repeat-containing 6
Synonyms: apollon, Bruce, A430032G04Rik, D630005A10Rik, A430040A19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 12211
Homologene: 7248
Chd9
Name: chromodomain helicase DNA binding protein 9
Synonyms: 1810014J18Rik, AD013, A330063D19Rik, 9030205D12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 109151
Homologene: 11844
Sorbs1
Name: sorbin and SH3 domain containing 1
Synonyms: c-Cbl-associated protein, CAP, Sh3d5, 9530001P15Rik, 2310065E01Rik, Ponsin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 20411
VEGA: 19
Homologene: 83252
Resf1
Name: retroelement silencing factor 1
Synonyms: GET, 2810474O19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 67246
Homologene: 19251
Cep170
Name: centrosomal protein 170
Synonyms: 4933426L22Rik, A330004A13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 545389
Homologene: 22844
Lgr4
Name: leucine-rich repeat-containing G protein-coupled receptor 4
Synonyms: A930009A08Rik, Gpr48, A330106J01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 107515
Homologene: 10226
Mark3
Name: MAP/microtubule affinity regulating kinase 3
Synonyms: ETK-1, 1600015G02Rik, A430080F22Rik, C-TAK1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 17169
VEGA: 12
HGNC: HGNC:6897
Homologene: 55653
Bcam
Name: basal cell adhesion molecule
Synonyms: B-CAM, 1200005K12Rik, Lu
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 57278
HGNC: HGNC:6722
Homologene: 21149
Vps13d
Name: vacuolar protein sorting 13D
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230895
Homologene: 15583
Mxra8
Name: matrix-remodelling associated 8
Synonyms: 1200013A08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 74761
HGNC: HGNC:7542
Homologene: 11500
Olfm3
Name: olfactomedin 3
Synonyms: optimedin, B230206G02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229759
Homologene: 17103
Tmod3
Name: tropomodulin 3
Synonyms: UTMOD, U-Tmod, ubiquitous tropomodulin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 50875
VEGA: 9
Homologene: 8727
Copg1
Name: coatomer protein complex, subunit gamma 1
Synonyms: D6Ertd71e, D6Wsu16e, Copg
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 54161
HGNC: HGNC:2236
Homologene: 56745
Eml2
Name: echinoderm microtubule associated protein like 2
Synonyms: 1600029N02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 72205
Homologene: 8125
Ndfip2
Name: Nedd4 family interacting protein 2
Synonyms: 0710001O20Rik, 9130207N19Rik, N4wbp5a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 76273
Homologene: 12636
A830010M20Rik
Name: RIKEN cDNA A830010M20 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231570
VEGA: 5
Homologene: 128318
Prickle1
Name: prickle planar cell polarity protein 1
Synonyms: 1110058P22Rik, mpk1, b2b019Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 106042
Homologene: 17686
Csk
Name: c-src tyrosine kinase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 12988
VEGA: 9
HGNC: HGNC:2444
Homologene: 55818
Ercc5
Name: excision repair cross-complementing rodent repair deficiency, complementation group 5
Synonyms: Xpg
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 22592
HGNC: HGNC:3437
Homologene: 133551
Reln
Name: reelin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 19699
HGNC: HGNC:9957
Homologene: 3699
Hps3
Name: HPS3, biogenesis of lysosomal organelles complex 2 subunit 1
Synonyms: Hermansky-Pudlak syndrome 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 12807
Homologene: 13019
Zfp831
Name: zinc finger protein 831
Synonyms: ENSMUSG00000050600, OTTMUSG00000017459
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 100043757
Homologene: 19013
Vmn1r28
Name: vomeronasal 1 receptor 28
Synonyms: V1rc25
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 171198
Homologene: 138094
Mapre2
Name: microtubule-associated protein, RP/EB family, member 2
Synonyms: D18Abb1e, RP1, EB2, C820009F03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 212307
HGNC: HGNC:6891
Homologene: 134539
Zfp608
Name: zinc finger protein 608
Synonyms: 4932417D18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 269023
VEGA: 18
Homologene: 18485
Wdr17
Name: WD repeat domain 17
Synonyms: 3010002I12Rik, B230207L18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 244484
Homologene: 12460
Unc13c
Name: unc-13 homolog C
Synonyms: Munc13-3, Unc13h3, D9Ertd414e, 1500037O19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 208898
Homologene: 45443
Vmn2r3
Name: vomeronasal 2, receptor 3
Synonyms: EG637004
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 637004
Vmn1r73
Name: vomeronasal 1 receptor 73
Synonyms: V1rg2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 171237
Homologene: 103842
Tdrd6
Name: tudor domain containing 6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 210510
Homologene: 19364
Nek11
Name: NIMA (never in mitosis gene a)-related expressed kinase 11
Synonyms: 4932416N14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 208583
Homologene: 57008
Myoz1
Name: myozenin 1
Synonyms: FATZ, calsarcin-2, 2310001N11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 59011
VEGA: 14
Homologene: 23311
Scgb2b2
Name: secretoglobin, family 2B, member 2
Synonyms: Abpe, Abpbg2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 381970
Homologene: 83171
1700006A11Rik
Name: RIKEN cDNA 1700006A11 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 71824
Nup210
Name: nucleoporin 210
Synonyms: gp210, gp190, Pom210
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 54563
Homologene: 41286
Krt76
Name: keratin 76
Synonyms: 2310001L23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 77055
Homologene: 22931
Zfp108
Name: zinc finger protein 108
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 54678
Homologene: 117959
Nin
Name: ninein
Synonyms: 3110068G20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 18080
Homologene: 40632
Smarca2
Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2
Synonyms: brm, Snf2l2, 2610209L14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 67155
Homologene: 2308
Tmem163
Name: transmembrane protein 163
Synonyms: 2610024A01Rik, SV31
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 72160
Homologene: 12798
Fsd1
Name: fibronectin type 3 and SPRY domain-containing protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 240121
VEGA: 17
Homologene: 11531
Cdca3
Name: cell division cycle associated 3
Synonyms: 2410005A12Rik, TOME-1, Grcc8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 14793
Homologene: 8403
Dffb
Name: DNA fragmentation factor, beta subunit
Synonyms: Didff, 40kDa, 5730477D02Rik, DFF40, CPAN, caspase-activated DNase, CAD
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 13368
HGNC: HGNC:2773
Homologene: 3241
Zkscan16
Name: zinc finger with KRAB and SCAN domains 16
Synonyms: Zfp483
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 100041581
Homologene: 26419
Vmn2r112
Name: vomeronasal 2, receptor 112
Synonyms: EG628185
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 628185
Homologene: 86604
Grip1
Name: glutamate receptor interacting protein 1
Synonyms: 4931400F03Rik, eb
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 74053
Homologene: 12938
C130050O18Rik
Name: RIKEN cDNA C130050O18 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 319772
Homologene: 138442
Kcna3
Name: potassium voltage-gated channel, shaker-related subfamily, member 3
Synonyms: Kv1.3, Kca1-3, Mk-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 16491
HGNC: HGNC:6221
Homologene: 128570
Or1e1f
Name: olfactory receptor family 1 subfamily E member 1F
Synonyms: GA_x6K02T2P1NL-4121434-4122381, MOR135-28, Olfr397
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 258343
Homologene: 138309
Best3
Name: bestrophin 3
Synonyms: mBest4, Vmd2l3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 382427
VEGA: 10
Homologene: 33850
Acaa1a
Name: acetyl-Coenzyme A acyltransferase 1A
Synonyms: peroxisomal 3-ketoacyl-CoA thiolase, PTL, D9Ertd25e, Acaa1, thiolase A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 113868
HGNC: HGNC:82
Homologene: 37497
Gm10242
Name: predicted gene 10242
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 100040589
Zfp94
Name: zinc finger protein 94
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 22756
Homologene: 86846
Hbq1b
Name: hemoglobin, theta 1B
Synonyms: F830116E18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 544763
HGNC: HGNC:4833
Homologene: 3895
Saxo2
Name: stabilizer of axonemal microtubules 2
Synonyms: 1700129I04Rik, Fam154b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 330577
Homologene: 25135
Mrfap1
Name: Morf4 family associated protein 1
Synonyms: PAM14, 9130413I22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 67568
Homologene: 128357
Ttc39c
Name: tetratricopeptide repeat domain 39C
Synonyms: 1700008N02Rik, 2810439F02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 72747
Homologene: 124438
Gm5414
Name: predicted gene 5414
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 406223
VEGA: 15
Ighj2
Name: immunoglobulin heavy joining 2
Synonyms: Gm16879
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 777654
Gm7356
Name: predicted gene 7356
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 664821
Homologene: 133217
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 44,157,057 bp
  • G to A, chromosome 1 at 127,491,625 bp
  • A to C, chromosome 1 at 127,996,827 bp
  • A to G, chromosome 1 at 176,739,125 bp
  • G to A, chromosome 2 at 26,962,392 bp
  • A to T, chromosome 2 at 110,006,502 bp
  • G to T, chromosome 2 at 174,644,624 bp
  • C to A, chromosome 3 at 20,014,173 bp
  • T to G, chromosome 3 at 64,259,197 bp
  • T to C, chromosome 3 at 107,037,891 bp
  • A to G, chromosome 3 at 115,101,940 bp
  • T to C, chromosome 3 at 124,419,856 bp
  • T to C, chromosome 4 at 19,552,289 bp
  • A to C, chromosome 4 at 58,946,506 bp
  • A to G, chromosome 4 at 134,806,596 bp
  • A to T, chromosome 4 at 145,155,857 bp
  • T to A, chromosome 4 at 153,965,407 bp
  • A to G, chromosome 4 at 155,840,904 bp
  • A to G, chromosome 5 at 21,925,193 bp
  • A to G, chromosome 5 at 36,796,745 bp
  • AGTGTGT to AGTGT, chromosome 5 at 73,068,782 bp
  • A to G, chromosome 5 at 107,508,817 bp
  • A to T, chromosome 5 at 110,787,589 bp
  • A to G, chromosome 5 at 114,111,960 bp
  • A to T, chromosome 5 at 139,414,389 bp
  • A to G, chromosome 6 at 48,939,877 bp
  • A to T, chromosome 6 at 58,265,540 bp
  • T to C, chromosome 6 at 87,887,996 bp
  • G to A, chromosome 6 at 91,017,529 bp
  • C to G, chromosome 6 at 124,822,630 bp
  • C to T, chromosome 6 at 124,833,014 bp
  • C to G, chromosome 6 at 149,329,389 bp
  • T to A, chromosome 7 at 11,756,669 bp
  • T to A, chromosome 7 at 19,193,999 bp
  • T to C, chromosome 7 at 19,763,995 bp
  • T to A, chromosome 7 at 24,261,314 bp
  • A to G, chromosome 7 at 24,303,741 bp
  • G to T, chromosome 7 at 27,306,116 bp
  • T to A, chromosome 7 at 31,303,631 bp
  • T to A, chromosome 7 at 82,634,535 bp
  • T to C, chromosome 8 at 54,629,861 bp
  • A to T, chromosome 8 at 91,034,230 bp
  • T to A, chromosome 8 at 113,641,953 bp
  • G to A, chromosome 9 at 42,993,065 bp
  • A to G, chromosome 9 at 57,630,780 bp
  • T to A, chromosome 9 at 73,574,022 bp
  • A to G, chromosome 9 at 75,532,448 bp
  • T to C, chromosome 9 at 105,287,658 bp
  • C to A, chromosome 9 at 119,342,761 bp
  • G to T, chromosome 10 at 88,771,960 bp
  • A to G, chromosome 10 at 117,008,981 bp
  • G to A, chromosome 10 at 119,931,248 bp
  • T to A, chromosome 11 at 32,287,014 bp
  • A to T, chromosome 11 at 73,965,340 bp
  • A to T, chromosome 12 at 70,044,063 bp
  • T to C, chromosome 12 at 82,396,678 bp
  • T to A, chromosome 12 at 111,592,653 bp
  • T to A, chromosome 12 at 113,429,480 bp
  • A to T, chromosome 14 at 20,649,538 bp
  • A to C, chromosome 14 at 87,007,199 bp
  • C to T, chromosome 14 at 105,258,685 bp
  • A to G, chromosome 15 at 93,500,548 bp
  • A to G, chromosome 15 at 101,625,010 bp
  • T to A, chromosome 15 at 101,888,162 bp
  • A to G, chromosome 17 at 14,001,236 bp
  • A to G, chromosome 17 at 22,603,382 bp
  • A to C, chromosome 17 at 43,624,327 bp
  • C to T, chromosome 17 at 55,991,241 bp
  • T to A, chromosome 17 at 74,565,905 bp
  • A to T, chromosome 18 at 12,734,894 bp
  • A to G, chromosome 18 at 23,832,933 bp
  • A to G, chromosome 18 at 54,946,591 bp
  • C to T, chromosome 19 at 26,678,346 bp
  • T to C, chromosome 19 at 40,311,727 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4912 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042514-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.