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Strain Name:
C57BL/6J-MtgxR5076Btlr/Mmmh
Stock Number:
042665-MU
Citation ID:
RRID:MMRRC_042665-MU
Other Names:
R5076 (G1), C57BL/6J-MtgxR5076Btlr
Major Collection:

Strain Information

Capzb
Name: capping actin protein of muscle Z-line subunit beta
Synonyms: CPbeta2, CPB2, CPbeta1, CPB1, 1700120C01Rik, Cappb1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 12345
HGNC: HGNC:1491
Homologene: 3620
Pitpnc1
Name: phosphatidylinositol transfer protein, cytoplasmic 1
Synonyms: C330017I21Rik, 5830436L09Rik, 1110020B03Rik, RDGB-BETA, RDGBB1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 71795
Homologene: 138301
Pdss1
Name: prenyl (solanesyl) diphosphate synthase, subunit 1
Synonyms: 2610203G20Rik, 2700031G06Rik, Tprt, mSPS1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 56075
Homologene: 5353
Mllt6
Name: myeloid/lymphoid or mixed-lineage leukemia; translocated to, 6
Synonyms: Af17
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 246198
HGNC: HGNC:7138
Homologene: 31347
Itpr1
Name: inositol 1,4,5-trisphosphate receptor 1
Synonyms: P400, IP3R1, Itpr-1, Ip3r, Pcp-1, opt, InsP3R type I, Pcp1, wblo
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 16438
HGNC: HGNC:6180
Homologene: 1673
Zfyve1
Name: zinc finger, FYVE domain containing 1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 217695
VEGA: 12
Homologene: 10945
Mrps5
Name: mitochondrial ribosomal protein S5
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 77721
Homologene: 32726
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • GT to GTT, chromosome 1 at 88,266,524 bp
  • A to T, chromosome 1 at 127,500,276 bp
  • A to G, chromosome 1 at 181,757,234 bp
  • A to T, chromosome 1 at 194,948,030 bp
  • A to G, chromosome 2 at 22,899,917 bp
  • A to G, chromosome 2 at 25,085,054 bp
  • T to C, chromosome 2 at 32,633,448 bp
  • T to A, chromosome 2 at 89,721,401 bp
  • T to C, chromosome 2 at 103,041,077 bp
  • A to T, chromosome 2 at 112,008,592 bp
  • T to A, chromosome 2 at 127,600,852 bp
  • T to A, chromosome 2 at 160,909,318 bp
  • G to A, chromosome 3 at 28,782,737 bp
  • C to T, chromosome 3 at 87,802,629 bp
  • A to G, chromosome 3 at 90,056,252 bp
  • G to A, chromosome 3 at 95,295,399 bp
  • T to A, chromosome 3 at 97,167,989 bp
  • A to G, chromosome 3 at 106,202,597 bp
  • A to T, chromosome 3 at 116,579,540 bp
  • T to A, chromosome 4 at 21,874,990 bp
  • C to T, chromosome 4 at 57,040,984 bp
  • A to T, chromosome 4 at 116,599,021 bp
  • A to T, chromosome 4 at 117,174,869 bp
  • A to T, chromosome 4 at 139,287,814 bp
  • A to G, chromosome 4 at 141,217,385 bp
  • G to A, chromosome 4 at 144,456,070 bp
  • T to A, chromosome 5 at 31,650,451 bp
  • A to T, chromosome 5 at 129,143,989 bp
  • T to A, chromosome 5 at 137,210,540 bp
  • A to G, chromosome 6 at 14,754,681 bp
  • T to C, chromosome 6 at 42,629,467 bp
  • A to G, chromosome 6 at 108,405,529 bp
  • C to T, chromosome 6 at 129,119,788 bp
  • A to G, chromosome 7 at 6,708,420 bp
  • A to T, chromosome 7 at 26,266,921 bp
  • T to A, chromosome 7 at 41,648,584 bp
  • A to G, chromosome 7 at 61,549,532 bp
  • G to A, chromosome 7 at 144,506,264 bp
  • T to G, chromosome 8 at 5,640,130 bp
  • C to T, chromosome 8 at 28,694,676 bp
  • T to A, chromosome 8 at 63,345,855 bp
  • C to A, chromosome 8 at 122,864,348 bp
  • T to A, chromosome 9 at 37,418,560 bp
  • T to C, chromosome 9 at 97,483,079 bp
  • G to T, chromosome 10 at 78,450,307 bp
  • C to T, chromosome 10 at 99,107,841 bp
  • G to A, chromosome 11 at 97,669,500 bp
  • C to T, chromosome 11 at 99,792,872 bp
  • A to T, chromosome 11 at 99,949,468 bp
  • A to T, chromosome 11 at 107,296,267 bp
  • A to G, chromosome 12 at 59,050,308 bp
  • T to C, chromosome 12 at 72,659,481 bp
  • C to T, chromosome 12 at 83,555,647 bp
  • C to A, chromosome 12 at 84,649,474 bp
  • C to T, chromosome 13 at 3,576,357 bp
  • T to A, chromosome 15 at 98,226,761 bp
  • A to G, chromosome 17 at 29,850,554 bp
  • T to C, chromosome 17 at 35,380,441 bp
  • G to A, chromosome 18 at 37,685,595 bp
  • G to T, chromosome 18 at 42,053,924 bp
  • T to C, chromosome 19 at 5,468,458 bp
  • T to C, chromosome 19 at 6,277,221 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5076 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042665-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.