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Strain Name:
C57BL/6J-MtgxR5253Btlr/Mmmh
Stock Number:
042824-MU
Citation ID:
RRID:MMRRC_042824-MU
Other Names:
R5253 (G1), C57BL/6J-MtgxR5253Btlr
Major Collection:

Strain Information

Kifc2
Name: kinesin family member C2
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 16581
VEGA: 15
Homologene: 7800
Etv1
Name: ets variant 1
Synonyms: ER81, Etsrp81
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 14009
HGNC: HGNC:3490
Homologene: 3636
Dusp1
Name: dual specificity phosphatase 1
Synonyms: mkp-1, erp, 3CH134, Ptpn16, MKP1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 19252
HGNC: HGNC:3064
Homologene: 3254
Pelp1
Name: proline, glutamic acid and leucine rich protein 1
Synonyms: 4930563C04Rik, MNAR
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 75273
Homologene: 8664
Hcn4
Name: hyperpolarization-activated, cyclic nucleotide-gated K+ 4
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 330953
VEGA: 9
Homologene: 3997
Rbm6
Name: RNA binding motif protein 6
Synonyms: NY-LU-12, g16, Def-3
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 19654
HGNC: HGNC:9903
Homologene: 31336
Smad2
Name: SMAD family member 2
Synonyms: Madr2, Smad 2, Madh2, 7120426M23Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 17126
HGNC: HGNC:6768
Homologene: 21197
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 2 at 24,719,952 bp
  • T to A, chromosome 2 at 25,737,143 bp
  • T to A, chromosome 2 at 30,032,363 bp
  • G to A, chromosome 2 at 76,791,551 bp
  • G to A, chromosome 2 at 87,418,668 bp
  • A to G, chromosome 2 at 88,988,100 bp
  • A to T, chromosome 2 at 89,156,457 bp
  • G to A, chromosome 2 at 158,129,438 bp
  • A to G, chromosome 3 at 93,200,812 bp
  • G to A, chromosome 3 at 93,910,791 bp
  • A to T, chromosome 4 at 123,323,093 bp
  • T to A, chromosome 4 at 143,398,586 bp
  • T to C, chromosome 4 at 150,638,270 bp
  • A to C, chromosome 4 at 154,667,569 bp
  • A to G, chromosome 5 at 30,370,139 bp
  • A to G, chromosome 5 at 96,741,025 bp
  • G to T, chromosome 5 at 125,026,930 bp
  • T to A, chromosome 6 at 29,070,863 bp
  • C to T, chromosome 6 at 29,498,520 bp
  • A to G, chromosome 6 at 85,445,171 bp
  • A to G, chromosome 6 at 118,597,969 bp
  • A to T, chromosome 6 at 121,888,913 bp
  • T to A, chromosome 6 at 128,619,163 bp
  • T to C, chromosome 6 at 139,896,257 bp
  • T to C, chromosome 7 at 22,761,758 bp
  • C to T, chromosome 7 at 26,450,492 bp
  • G to A, chromosome 7 at 43,692,077 bp
  • G to A, chromosome 7 at 72,673,517 bp
  • A to C, chromosome 7 at 101,388,644 bp
  • A to G, chromosome 7 at 101,822,105 bp
  • G to T, chromosome 7 at 112,861,545 bp
  • A to T, chromosome 7 at 123,303,748 bp
  • T to C, chromosome 8 at 26,072,291 bp
  • T to A, chromosome 8 at 48,229,198 bp
  • C to A, chromosome 8 at 88,200,023 bp
  • T to C, chromosome 8 at 88,314,114 bp
  • T to C, chromosome 8 at 110,883,867 bp
  • C to G, chromosome 8 at 111,349,237 bp
  • T to C, chromosome 8 at 121,618,694 bp
  • T to C, chromosome 9 at 40,051,450 bp
  • T to A, chromosome 9 at 58,824,275 bp
  • C to T, chromosome 9 at 107,852,657 bp
  • G to A, chromosome 10 at 76,299,997 bp
  • A to G, chromosome 10 at 79,247,986 bp
  • A to G, chromosome 10 at 79,920,750 bp
  • A to G, chromosome 10 at 86,730,942 bp
  • A to G, chromosome 10 at 129,691,732 bp
  • G to T, chromosome 11 at 36,047,201 bp
  • C to A, chromosome 11 at 70,401,661 bp
  • A to G, chromosome 11 at 77,473,235 bp
  • G to T, chromosome 11 at 114,894,751 bp
  • A to G, chromosome 12 at 38,852,249 bp
  • T to C, chromosome 13 at 23,875,850 bp
  • T to G, chromosome 13 at 55,011,011 bp
  • A to G, chromosome 14 at 29,021,844 bp
  • A to G, chromosome 14 at 33,945,307 bp
  • T to C, chromosome 14 at 50,035,288 bp
  • G to A, chromosome 14 at 79,602,901 bp
  • G to T, chromosome 15 at 76,666,281 bp
  • T to C, chromosome 15 at 102,111,453 bp
  • T to C, chromosome 17 at 26,508,217 bp
  • T to C, chromosome 17 at 37,055,913 bp
  • C to T, chromosome 17 at 86,495,940 bp
  • T to C, chromosome 18 at 20,272,379 bp
  • C to A, chromosome 18 at 32,269,864 bp
  • T to C, chromosome 18 at 37,753,097 bp
  • A to G, chromosome 18 at 76,288,053 bp
  • G to A, chromosome 19 at 4,750,082 bp
  • G to A, chromosome 19 at 8,344,393 bp
  • A to G, chromosome 19 at 32,674,302 bp
  • T to C, chromosome 19 at 40,607,241 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5253 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042824-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.