Strain Name:
C57BL/6J-MtgxR5253Btlr/Mmmh
Stock Number:
042824-MU
Citation ID:
RRID:MMRRC_042824-MU
Other Names:
R5253 (G1), C57BL/6J-MtgxR5253Btlr
Major Collection:

Gene Information

Kifc2
Name: kinesin family member C2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 16581
VEGA: 15
Homologene: 7800
Etv1
Name: ets variant 1
Synonyms: ER81, Etsrp81
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 14009
HGNC: HGNC:3490
Homologene: 3636
Dusp1
Name: dual specificity phosphatase 1
Synonyms: mkp-1, erp, 3CH134, Ptpn16, MKP1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 19252
HGNC: HGNC:3064
Homologene: 3254
Pelp1
Name: proline, glutamic acid and leucine rich protein 1
Synonyms: 4930563C04Rik, MNAR
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 75273
Homologene: 8664
Hcn4
Name: hyperpolarization-activated, cyclic nucleotide-gated K+ 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 330953
VEGA: 9
Homologene: 3997
Rbm6
Name: RNA binding motif protein 6
Synonyms: NY-LU-12, g16, Def-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 19654
HGNC: HGNC:9903
Homologene: 31336
Smad2
Name: SMAD family member 2
Synonyms: Madr2, Smad 2, Madh2, 7120426M23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 17126
HGNC: HGNC:6768
Homologene: 21197
Sugt1
Name: SGT1, suppressor of G2 allele of SKP1 (S. cerevisiae)
Synonyms: SGT1, 2410174K12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 67955
VEGA: 14
Homologene: 4877
Tead1
Name: TEA domain family member 1
Synonyms: mTEF-1, TEF-1, TEAD-1, Tcf13, Gtrgeo5, B230114H05Rik, 2610024B07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 21676
Homologene: 2418
Ncor2
Name: nuclear receptor co-repressor 2
Synonyms: SMRTe, SMRT
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 20602
HGNC: HGNC:7673
Homologene: 31370
Tenm2
Name: teneurin transmembrane protein 2
Synonyms: Ten-m2, D3Bwg1534e, 9330187F13Rik, 2610040L17Rik, Odz2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 23964
Homologene: 22672
Smyd5
Name: SET and MYND domain containing 5
Synonyms: NN8-4AG, Rrg1, Rai15
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 232187
Homologene: 6143
Otof
Name: otoferlin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 83762
HGNC: HGNC:8515
Homologene: 12892
Gabbr1
Name: gamma-aminobutyric acid (GABA) B receptor, 1
Synonyms: GABAbR1, GABAB1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 54393
HGNC: HGNC:4070
Homologene: 1132
Phox2a
Name: paired-like homeobox 2a
Synonyms: Pmx2, Pmx2a, Arix, Px2a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 11859
HGNC: HGNC:691
Homologene: 31296
Arhgap17
Name: Rho GTPase activating protein 17
Synonyms: WBP15, Nadrin, Rich1, 5730403H17Rik, Nadrin2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 70497
Homologene: 9984
Kansl2-ps
Name: KAT8 regulatory NSL complex subunit 2, pseudogene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233391
Cd300a
Name: CD300A molecule
Synonyms: Pigr4, MMAC8, Clm8, LMIR1, B230315M08Rik, MAIR-I
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 217303
Homologene: 48514
Sptbn2
Name: spectrin beta, non-erythrocytic 2
Synonyms: Spnb3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 20743
VEGA: 19
Homologene: 48482
Tenm3
Name: teneurin transmembrane protein 3
Synonyms: Ten-m3, 2610100B16Rik, Odz3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 23965
Homologene: 22673
Atad1
Name: ATPase family, AAA domain containing 1
Synonyms: 4921525H23Rik, Thorase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 67979
VEGA: 19
Homologene: 5960
Arap1
Name: ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1
Synonyms: 2410002L19Rik, Centd2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 69710
Homologene: 12326
Tent4b
Name: terminal nucleotidyltransferase 4B
Synonyms: 5730445M16Rik, Papd5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 214627
Homologene: 64245
Dync2i2
Name: dynein 2 intermediate chain 2
Synonyms: 3200002I06Rik, Wdr34
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 71820
Homologene: 14156
Lep
Name: leptin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 16846
HGNC: HGNC:6553
Homologene: 193
Cacna1c
Name: calcium channel, voltage-dependent, L type, alpha 1C subunit
Synonyms: (alpha)1 subunit, Cchl1a1, Cav1.2, L-type Cav1.2, D930026N18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 12288
HGNC: HGNC:1390
Homologene: 55484
Tns2
Name: tensin 2
Synonyms: nep, nph, Tenc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 209039
VEGA: 15
Homologene: 37077
Ttn
Name: titin
Synonyms: connectin, L56, mdm, 1100001C23Rik, D830007G01Rik, 2310036G12Rik, 2310074I15Rik, 2310057K23Rik, D330041I19Rik, shru
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 22138
Homologene: 130650
Nlrp4a
Name: NLR family, pyrin domain containing 4A
Synonyms: Nalp-eta, E330028A19Rik, Nalp4a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 243880
Homologene: 79696
Zcchc14
Name: zinc finger, CCHC domain containing 14
Synonyms: Bdg29
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 142682
Homologene: 9037
Pramel1
Name: PRAME like 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 83491
Homologene: 87078
Kcp
Name: kielin/chordin-like protein
Synonyms: LOC333088, KCP, Crim2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 333088
Homologene: 87817
Pik3c2g
Name: phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 gamma
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 18705
HGNC: HGNC:8973
Homologene: 3362
Fcsk
Name: fucose kinase
Synonyms: L-fucose kinase, 1110046B12Rik, Fuk
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234730
Homologene: 15452
Fa2h
Name: fatty acid 2-hydroxylase
Synonyms: G630055L08Rik, Faxdc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 338521
Homologene: 56284
Slc45a1
Name: solute carrier family 45, member 1
Synonyms: Dnb5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 242773
Homologene: 44908
Obp2b
Name: odorant binding protein 2B
Synonyms: OTTMUSG00000012631, Lcn14
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 383678
Homologene: 135821
Olfr1214
Name: olfactory receptor 1214
Synonyms: GA_x6K02T2Q125-50468705-50467770, MOR233-8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258899
Homologene: 73984
Fras1
Name: Fraser extracellular matrix complex subunit 1
Synonyms: bl, E130113P14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231470
Homologene: 23516
Cacna1b
Name: calcium channel, voltage-dependent, N type, alpha 1B subunit
Synonyms: Cchn1a, Cav2.2, alpha(1B)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 12287
HGNC: HGNC:1389
Homologene: 20184
Prkce
Name: protein kinase C, epsilon
Synonyms: PKC[e], Pkce, 5830406C15Rik, PKCepsilon
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 18754
HGNC: HGNC:9401
Homologene: 48343
Ttc41
Name: tetratricopeptide repeat domain 41
Synonyms: Gnn, BC030307
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 103220
VEGA: 10
Homologene: 52968
Olfr1224-ps1
Name: olfactory receptor 1224, pseudogene 1
Synonyms: GA_x6K02T2Q125-50635980-50635046, MOR233-15
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258026
Flg2
Name: filaggrin family member 2
Synonyms: EG229574
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229574
Homologene: 134146
Slc22a30
Name: solute carrier family 22, member 30
Synonyms: C730048C13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 319800
Homologene: 77136
Mug1
Name: murinoglobulin 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 17836
Homologene: 136663
Lrtm1
Name: leucine-rich repeats and transmembrane domains 1
Synonyms: A930016D02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 319476
VEGA: 14
Homologene: 49635
Kiss1r
Name: KISS1 receptor
Synonyms: Gpr54
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 114229
VEGA: 10
HGNC: HGNC:4510
Homologene: 11411
Dsg1c
Name: desmoglein 1 gamma
Synonyms: Dsg6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 211924
HGNC: HGNC:3048
Gm9116
Name: predicted gene 9116
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 668344
Adcy7
Name: adenylate cyclase 7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 11513
HGNC: HGNC:238
Homologene: 866
Klk14
Name: kallikrein related-peptidase 14
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 317653
HGNC: HGNC:6362
Homologene: 69348
Ankrd13b
Name: ankyrin repeat domain 13b
Synonyms: B930093C12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 268445
Homologene: 27367
Ercc3
Name: excision repair cross-complementing rodent repair deficiency, complementation group 3
Synonyms: XPB
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 13872
HGNC: HGNC:3435
Homologene: 96
Tctn3
Name: tectonic family member 3
Synonyms: Tect3, 4930521E07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 67590
Homologene: 9221
Olfr725
Name: olfactory receptor 725
Synonyms: GA_x6K02T2PMLR-5725741-5724776, MOR246-3, MOR246-7_p
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 258314
Homologene: 44957
Dip2a
Name: disco interacting protein 2 homolog A
Synonyms: Kiaa0184-hp, 4931420H10Rik, Dip2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 64451
Homologene: 41012
Vmn2r81
Name: vomeronasal 2, receptor 81
Synonyms: pheromone recepter, EC1-VR2, V2rf2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 216144
Homologene: 83483
Hook3
Name: hook microtubule tethering protein 3
Synonyms: E330005F07Rik, 5830454D03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 320191
Homologene: 13022
Klrb1a
Name: killer cell lectin-like receptor subfamily B member 1A
Synonyms: Nkrp1-a, Ly55a, NKR-P1A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 17057
HGNC: HGNC:6373
Homologene: 84369
Hk3
Name: hexokinase 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 212032
HGNC: HGNC:4925
Homologene: 55633
Actrt2
Name: actin-related protein T2
Synonyms: Arp-T2, 1700052K15Rik, Arpm2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 73353
Homologene: 23647
Gdf2
Name: growth differentiation factor 2
Synonyms: Bmp9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 12165
VEGA: 14
HGNC: HGNC:4217
Homologene: 32299
Tgm2
Name: transglutaminase 2, C polypeptide
Synonyms: tissue transglutaminase, protein-glutamine gamma-glutamyltransferase, G[a]h, TG C, tTGas, tTG, TG2, TGase2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 21817
Homologene: 3391
Olfr1123
Name: olfactory receptor 1123
Synonyms: GA_x6K02T2Q125-48917235-48918206, MOR264-17
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258347
Homologene: 105187
Slc17a1
Name: solute carrier family 17 (sodium phosphate), member 1
Synonyms: Npt1, NAPI-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 20504
Homologene: 48324
Oxct2a
Name: 3-oxoacid CoA transferase 2A
Synonyms: Oxct2, Scot-t1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 64059
Homologene: 137359
Olfr803
Name: olfactory receptor 803
Synonyms: GA_x6K02T2PULF-11370664-11369738, MOR111-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 258547
Homologene: 105153
Pcdhgb7
Name: protocadherin gamma subfamily B, 7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 93704
HGNC: HGNC:8714
Homologene: 75102
1700001J11Rik
Name: RIKEN cDNA 1700001J11 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 72224
Vmn1r157
Name: vomeronasal 1 receptor 157
Synonyms: Gm4517, Gm8699, Vmn1r109
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 667551
Homologene: 104166
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 2 at 24,719,952 bp
  • T to A, chromosome 2 at 25,737,143 bp
  • T to A, chromosome 2 at 30,032,363 bp
  • G to A, chromosome 2 at 76,791,551 bp
  • G to A, chromosome 2 at 87,418,668 bp
  • A to G, chromosome 2 at 88,988,100 bp
  • A to T, chromosome 2 at 89,156,457 bp
  • G to A, chromosome 2 at 158,129,438 bp
  • A to G, chromosome 3 at 93,200,812 bp
  • G to A, chromosome 3 at 93,910,791 bp
  • A to T, chromosome 4 at 123,323,093 bp
  • T to A, chromosome 4 at 143,398,586 bp
  • T to C, chromosome 4 at 150,638,270 bp
  • A to C, chromosome 4 at 154,667,569 bp
  • A to G, chromosome 5 at 30,370,139 bp
  • A to G, chromosome 5 at 96,741,025 bp
  • G to T, chromosome 5 at 125,026,930 bp
  • T to A, chromosome 6 at 29,070,863 bp
  • C to T, chromosome 6 at 29,498,520 bp
  • A to G, chromosome 6 at 85,445,171 bp
  • A to G, chromosome 6 at 118,597,969 bp
  • A to T, chromosome 6 at 121,888,913 bp
  • T to A, chromosome 6 at 128,619,163 bp
  • T to C, chromosome 6 at 139,896,257 bp
  • T to C, chromosome 7 at 22,761,758 bp
  • C to T, chromosome 7 at 26,450,492 bp
  • G to A, chromosome 7 at 43,692,077 bp
  • G to A, chromosome 7 at 72,673,517 bp
  • A to C, chromosome 7 at 101,388,644 bp
  • A to G, chromosome 7 at 101,822,105 bp
  • G to T, chromosome 7 at 112,861,545 bp
  • A to T, chromosome 7 at 123,303,748 bp
  • T to C, chromosome 8 at 26,072,291 bp
  • T to A, chromosome 8 at 48,229,198 bp
  • C to A, chromosome 8 at 88,200,023 bp
  • T to C, chromosome 8 at 88,314,114 bp
  • T to C, chromosome 8 at 110,883,867 bp
  • C to G, chromosome 8 at 111,349,237 bp
  • T to C, chromosome 8 at 121,618,694 bp
  • T to C, chromosome 9 at 40,051,450 bp
  • T to A, chromosome 9 at 58,824,275 bp
  • C to T, chromosome 9 at 107,852,657 bp
  • G to A, chromosome 10 at 76,299,997 bp
  • A to G, chromosome 10 at 79,247,986 bp
  • A to G, chromosome 10 at 79,920,750 bp
  • A to G, chromosome 10 at 86,730,942 bp
  • A to G, chromosome 10 at 129,691,732 bp
  • G to T, chromosome 11 at 36,047,201 bp
  • C to A, chromosome 11 at 70,401,661 bp
  • A to G, chromosome 11 at 77,473,235 bp
  • G to T, chromosome 11 at 114,894,751 bp
  • A to G, chromosome 12 at 38,852,249 bp
  • T to C, chromosome 13 at 23,875,850 bp
  • T to G, chromosome 13 at 55,011,011 bp
  • A to G, chromosome 14 at 29,021,844 bp
  • A to G, chromosome 14 at 33,945,307 bp
  • T to C, chromosome 14 at 50,035,288 bp
  • G to A, chromosome 14 at 79,602,901 bp
  • G to T, chromosome 15 at 76,666,281 bp
  • T to C, chromosome 15 at 102,111,453 bp
  • T to C, chromosome 17 at 26,508,217 bp
  • T to C, chromosome 17 at 37,055,913 bp
  • C to T, chromosome 17 at 86,495,940 bp
  • T to C, chromosome 18 at 20,272,379 bp
  • C to A, chromosome 18 at 32,269,864 bp
  • T to C, chromosome 18 at 37,753,097 bp
  • A to G, chromosome 18 at 76,288,053 bp
  • G to A, chromosome 19 at 4,750,082 bp
  • G to A, chromosome 19 at 8,344,393 bp
  • A to G, chromosome 19 at 32,674,302 bp
  • T to C, chromosome 19 at 40,607,241 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
(Deceased) Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5253 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
042824-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.