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Strain Name:
C57BL/6J-MtgxR5428Btlr/Mmmh
Stock Number:
042994-MU
Citation ID:
RRID:MMRRC_042994-MU
Other Names:
R5428 (G1), C57BL/6J-MtgxR5428Btlr
Major Collection:

Strain Information

Utrn
Name: utrophin
Synonyms: G-utrophin, Dmdl, DRP
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 22288
VEGA: 10
Homologene: 21398
Map4k5
Name: mitogen-activated protein kinase kinase kinase kinase 5
Synonyms: MAPKKKK5, GCKR, KHS, 4432415E19Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 399510
HGNC: HGNC:6867
Homologene: 38199
Grm1
Name: glutamate receptor, metabotropic 1
Synonyms: mGluR1, Grm1, Gprc1a, nmf373, rcw, 4930455H15Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 14816
HGNC: HGNC:4593
Homologene: 649
Macf1
Name: microtubule-actin crosslinking factor 1
Synonyms: Acf7, Aclp7, trabeculin alpha
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11426
Homologene: 136191
Orc1
Name: origin recognition complex, subunit 1
Synonyms: MmORC1, Orc1l
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 18392
HGNC: HGNC:8487
Homologene: 31221
Foxp1
Name: forkhead box P1
Synonyms: 4932443N09Rik, 3110052D19Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 108655
HGNC: HGNC:3823
Homologene: 13092
Ylpm1
Name: YLP motif containing 1
Synonyms: ZAP, Zap3, A930013E17Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 56531
Homologene: 87707
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 4,880,345 bp
  • C to A, chromosome 1 at 10,160,835 bp
  • T to C, chromosome 1 at 156,642,111 bp
  • T to C, chromosome 1 at 158,814,785 bp
  • A to G, chromosome 2 at 28,450,626 bp
  • A to G, chromosome 2 at 36,245,268 bp
  • T to C, chromosome 2 at 76,761,104 bp
  • A to T, chromosome 2 at 76,885,136 bp
  • C to T, chromosome 2 at 84,616,789 bp
  • A to G, chromosome 2 at 112,367,355 bp
  • A to G, chromosome 2 at 131,561,403 bp
  • C to T, chromosome 2 at 146,334,494 bp
  • T to A, chromosome 3 at 31,097,498 bp
  • G to A, chromosome 3 at 151,542,686 bp
  • C to T, chromosome 4 at 3,905,538 bp
  • T to A, chromosome 4 at 32,601,169 bp
  • A to T, chromosome 4 at 45,106,689 bp
  • T to A, chromosome 4 at 108,599,940 bp
  • A to G, chromosome 4 at 123,384,868 bp
  • G to A, chromosome 4 at 154,145,274 bp
  • T to C, chromosome 5 at 134,625,379 bp
  • T to G, chromosome 5 at 150,405,359 bp
  • T to C, chromosome 6 at 90,223,413 bp
  • A to G, chromosome 6 at 99,016,631 bp
  • A to G, chromosome 6 at 130,630,564 bp
  • T to C, chromosome 7 at 83,612,547 bp
  • C to T, chromosome 7 at 128,704,524 bp
  • A to G, chromosome 7 at 139,908,962 bp
  • G to T, chromosome 7 at 143,479,345 bp
  • A to G, chromosome 8 at 21,135,403 bp
  • A to G, chromosome 8 at 70,784,733 bp
  • G to T, chromosome 8 at 71,622,776 bp
  • T to C, chromosome 8 at 84,676,154 bp
  • A to T, chromosome 8 at 85,540,175 bp
  • A to G, chromosome 9 at 13,705,895 bp
  • T to A, chromosome 10 at 10,719,563 bp
  • T to C, chromosome 10 at 12,693,431 bp
  • C to T, chromosome 10 at 20,023,653 bp
  • T to C, chromosome 10 at 41,464,788 bp
  • A to C, chromosome 10 at 83,574,522 bp
  • G to A, chromosome 10 at 99,445,147 bp
  • G to A, chromosome 10 at 108,253,347 bp
  • A to G, chromosome 11 at 67,700,049 bp
  • T to A, chromosome 11 at 72,030,487 bp
  • A to G, chromosome 11 at 117,700,762 bp
  • G to A, chromosome 12 at 29,832,332 bp
  • A to G, chromosome 12 at 57,689,834 bp
  • T to C, chromosome 12 at 69,838,013 bp
  • T to C, chromosome 12 at 85,030,229 bp
  • A to G, chromosome 14 at 98,169,269 bp
  • T to A, chromosome 14 at 120,478,994 bp
  • T to C, chromosome 15 at 8,330,296 bp
  • C to G, chromosome 15 at 55,642,795 bp
  • C to A, chromosome 15 at 76,350,666 bp
  • T to A, chromosome 15 at 78,428,782 bp
  • A to C, chromosome 15 at 85,862,139 bp
  • T to C, chromosome 16 at 17,558,261 bp
  • T to C, chromosome 16 at 90,991,518 bp
  • A to T, chromosome 17 at 24,181,772 bp
  • A to G, chromosome 17 at 64,712,300 bp
  • G to C, chromosome 18 at 6,635,918 bp
  • A to T, chromosome 18 at 46,926,320 bp
  • T to G, chromosome Y at 726,205 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5428 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042994-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.