Strain Name:
C57BL/6J-MtgxR5447Btlr/Mmmh
Stock Number:
043012-MU
Citation ID:
RRID:MMRRC_043012-MU
Other Names:
R5447 (G1), C57BL/6J-MtgxR5447Btlr
Major Collection:

Gene Information

Rptor
Name: regulatory associated protein of MTOR, complex 1
Synonyms: raptor, Rap, 4932417H02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 74370
Homologene: 80210
Dysf
Name: dysferlin
Synonyms: 2310004N10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 26903
HGNC: HGNC:3097
Homologene: 20748
Ptch1
Name: patched 1
Synonyms: Ptc1, Patched 1, A230106A15Rik, wig, Ptc
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 19206
HGNC: HGNC:9585
Homologene: 223
Stam2
Name: signal transducing adaptor molecule (SH3 domain and ITAM motif) 2
Synonyms: Hbp, 5730456G07Rik, 1200004O12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 56324
Homologene: 68490
Adgrl3
Name: adhesion G protein-coupled receptor L3
Synonyms: D130075K09Rik, 5430402I23Rik, LEC3, lectomedin 3, Lphn3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 319387
Homologene: 22878
Helb
Name: helicase (DNA) B
Synonyms: D10Ertd664e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 117599
Homologene: 50463
Dip2b
Name: disco interacting protein 2 homolog B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 239667
Homologene: 72227
Zgrf1
Name: zinc finger, GRF-type containing 1
Synonyms: 4930422G04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 71643
Homologene: 34708
Mdn1
Name: midasin AAA ATPase 1
Synonyms: LOC213784, D4Abb1e, 4833432B22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 100019
Homologene: 39689
Snw1
Name: SNW domain containing 1
Synonyms: SKIP, 2310008B08Rik, SNW1, Skiip, NCoA-62
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 66354
VEGA: 12
Homologene: 56557
Vps39
Name: VPS39 HOPS complex subunit
Synonyms: Vam6, Vam6P, A230065P22Rik, mVam6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 269338
Homologene: 41025
Cyfip2
Name: cytoplasmic FMR1 interacting protein 2
Synonyms: Pir121, 6430511D02Rik, 1500004I01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 76884
Homologene: 7936
Nfs1
Name: nitrogen fixation gene 1 (S. cerevisiae)
Synonyms: m-Nfs1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 18041
Homologene: 5463
Eef1aknmt
Name: EEF1A lysine and N-terminal methyltransferase
Synonyms: 5630401D24Rik, Mettl13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 71449
Homologene: 32285
Dmbt1
Name: deleted in malignant brain tumors 1
Synonyms: gp300, Crpd, hensin, ebnerin, CRP-[a], vomeroglandin, CRP-[b], MUCLIN
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 12945
HGNC: HGNC:2926
Homologene: 68990
Robo2
Name: roundabout guidance receptor 2
Synonyms: 2600013A04Rik, 9430089E08Rik, D230004I22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 268902
Homologene: 43188
Zfp141
Name: zinc finger protein 141
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 434178
Pdgfrb
Name: platelet derived growth factor receptor, beta polypeptide
Synonyms: Pdgfr, CD140b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 18596
HGNC: HGNC:8804
Homologene: 1960
Adam30
Name: a disintegrin and metallopeptidase domain 30
Synonyms: 4933424D07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 71078
HGNC: HGNC:208
Homologene: 11038
E130114P18Rik
Name: RIKEN cDNA E130114P18 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 319865
Ptprs
Name: protein tyrosine phosphatase, receptor type, S
Synonyms: PTP-NU3, PTPsigma, Ptpt9, RPTPsigma
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 19280
HGNC: HGNC:9681
Homologene: 20626
Mapk8ip3
Name: mitogen-activated protein kinase 8 interacting protein 3
Synonyms: Syd2, c-Jun NH2-terminal kinase (JNK)/stress-activated protein kinase-associated protein 1, JSAP1a, JSAP1b, JSAP1c, JSAP1d, JUN/SAPK-associated protein 1, Jip3, JNK-interacting protein 3, JSAP1, D17Wsu15e, sunday driver 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 30957
HGNC: HGNC:6884
Homologene: 22790
Trmt10b
Name: tRNA methyltransferase 10B
Synonyms: Rg9mtd3, 2610042J10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 69934
Homologene: 12340
Nup160
Name: nucleoporin 160
Synonyms: Gtl1-13, 2810011M03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 59015
Homologene: 32509
Baz2b
Name: bromodomain adjacent to zinc finger domain, 2B
Synonyms: 5830435C13Rik, D2Ertd794e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 407823
HGNC: HGNC:963
Homologene: 8394
Prol1
Name: proline rich, lacrimal 1
Synonyms: Muc10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 17830
Hoxd4
Name: homeobox D4
Synonyms: Hox-5.1, Hox-4.2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 15436
HGNC: HGNC:5138
Homologene: 7773
Mmgt2
Name: membrane magnesium transporter 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 216829
Homologene: 18391
Ttn
Name: titin
Synonyms: shru, connectin, mdm, 2310057K23Rik, D330041I19Rik, 1100001C23Rik, D830007G01Rik, 2310036G12Rik, L56, 2310074I15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 22138
Homologene: 130650
Pkhd1
Name: polycystic kidney and hepatic disease 1
Synonyms: FPC, tigmin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 241035
HGNC: HGNC:9016
Homologene: 16336
Muc4
Name: mucin 4
Synonyms: 4933405I11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 140474
HGNC: HGNC:7514
Homologene: 124469
Stk10
Name: serine/threonine kinase 10
Synonyms: Lok, Gek1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 20868
Homologene: 38122
Cdh5
Name: cadherin 5
Synonyms: VEC, CD144, VEcad, VE-cadherin, 7B4/cadherin-5, VE-Cad, VECD
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 12562
HGNC: HGNC:1764
Homologene: 1359
Neu4
Name: sialidase 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 241159
Homologene: 15433
BC016579
Name: cDNA sequence, BC016579
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 212998
VEGA: 16
Homologene: 11622
Zan
Name: zonadhesin
Synonyms: Zan
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 22635
Homologene: 124417
Slc4a11
Name: solute carrier family 4, sodium bicarbonate transporter-like, member 11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 269356
Homologene: 12931
Pear1
Name: platelet endothelial aggregation receptor 1
Synonyms: 3110045G13Rik, Jedi-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 73182
Homologene: 12492
Scara5
Name: scavenger receptor class A, member 5
Synonyms: 4933425F03Rik, 4932433F15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 71145
Homologene: 12556
Cdhr2
Name: cadherin-related family member 2
Synonyms: Pcdh24, LOC268663
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 268663
Homologene: 134510
Skint6
Name: selection and upkeep of intraepithelial T cells 6
Synonyms: OTTMUSG00000008519
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 230622
Homologene: 135888
Abcb5
Name: ATP-binding cassette, sub-family B (MDR/TAP), member 5
Synonyms: 9230106F14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 77706
HGNC: HGNC:46
Homologene: 83488
Il1rl2
Name: interleukin 1 receptor-like 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 107527
HGNC: HGNC:5999
Homologene: 2860
Myo15b
Name: myosin XVB
Synonyms: LOC380737, LOC217328, E330039G21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 217328
Albfm1
Name: albumin superfamily member 1
Synonyms: ARG, Gm17754, 5830473C10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 622307
Homologene: 129803
Ppp4r4
Name: protein phosphatase 4, regulatory subunit 4
Synonyms: 8430415E04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 74521
Homologene: 12571
B4galnt3
Name: beta-1,4-N-acetyl-galactosaminyl transferase 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 330406
Homologene: 18328
Tmc3
Name: transmembrane channel-like gene family 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 233424
Homologene: 45588
Gemin6
Name: gem nuclear organelle associated protein 6
Synonyms: 2810470M17Rik, 2610019B15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 67242
Homologene: 11711
Olfr381
Name: olfactory receptor 381
Synonyms: GA_x6K02T2P1NL-3646409-3645474, MOR135-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 259024
Proz
Name: protein Z, vitamin K-dependent plasma glycoprotein
Synonyms: 1300015B06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 66901
HGNC: HGNC:9460
Homologene: 2890
Adrb1
Name: adrenergic receptor, beta 1
Synonyms: beta 1-AR, Adrb-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 11554
VEGA: 19
HGNC: HGNC:285
Homologene: 20171
Olfr605
Name: olfactory receptor 605
Synonyms: MOR24-5, GA_x6K02T2PBJ9-6164792-6163848, MOR202-22P
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 258156
Homologene: 79414
Fam110a
Name: family with sequence similarity 110, member A
Synonyms: 1700008J10Rik, 5430432M24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 73847
Homologene: 12862
Nid1
Name: nidogen 1
Synonyms: nidogen-1, entactin-1, entactin 1, entactin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 18073
VEGA: 13
HGNC: HGNC:7821
Homologene: 1878
Olfr51
Name: olfactory receptor 51
Synonyms: MOR129-3, ID7, GA_x6K02T2QP88-4431129-4430206
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 18351
Homologene: 133578
Btnl10
Name: butyrophilin-like 10
Synonyms: Butr1, BUTR-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 192194
Homologene: 138184
Sp110
Name: Sp110 nuclear body protein
Synonyms: Ifi75, Ipr1, 5031415C07Rik, 5830484A20Rik, 52kDa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 109032
HGNC: HGNC:5401
Homologene: 82192
Nfxl1
Name: nuclear transcription factor, X-box binding-like 1
Synonyms: LOC381696, TCF9, 1700012H24Rik, D430033A06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 100978
Homologene: 26752
Gpsm2
Name: G-protein signalling modulator 2 (AGS3-like, C. elegans)
Synonyms: Pins, 6230410J09Rik, LGN
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 76123
Homologene: 56584
Olfr175-ps1
Name: olfactory receptor 175, pseudogene 1
Synonyms: GA_x54KRFPKG5P-55026345-55025418, GA_x54KRFPKG5P-54993816-54992890, MOR184-10P, Olfr174, MOR184-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 259004
Homologene: 17461
Lhfpl5
Name: lipoma HMGIC fusion partner-like 5
Synonyms: 9330179O15Rik, Tmhs
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 328789
Homologene: 18794
Mylk2
Name: myosin, light polypeptide kinase 2, skeletal muscle
Synonyms: 9830004H17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 228785
Homologene: 13223
H2-Ke6
Name: H2-K region expressed gene 6
Synonyms: Ring2, H-2Ke6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 14979
HGNC: HGNC:3554
Homologene: 56588
Clk2
Name: CDC-like kinase 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 12748
HGNC: HGNC:2069
Homologene: 33303
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 20,239,385 bp
  • T to G, chromosome 1 at 40,329,156 bp
  • C to G, chromosome 1 at 85,589,118 bp
  • C to T, chromosome 1 at 94,022,418 bp
  • A to G, chromosome 1 at 162,535,880 bp
  • G to A, chromosome 2 at 52,736,293 bp
  • T to C, chromosome 2 at 59,913,988 bp
  • A to G, chromosome 2 at 74,727,343 bp
  • A to T, chromosome 2 at 76,811,243 bp
  • T to A, chromosome 2 at 76,899,107 bp
  • C to A, chromosome 2 at 90,725,615 bp
  • T to C, chromosome 2 at 120,352,932 bp
  • T to C, chromosome 2 at 130,691,742 bp
  • T to C, chromosome 2 at 151,970,709 bp
  • T to C, chromosome 2 at 152,912,510 bp
  • C to T, chromosome 2 at 156,142,136 bp
  • G to A, chromosome 3 at 87,759,142 bp
  • G to T, chromosome 3 at 89,167,191 bp
  • A to G, chromosome 3 at 98,161,343 bp
  • G to A, chromosome 3 at 108,702,936 bp
  • T to C, chromosome 3 at 127,563,119 bp
  • T to C, chromosome 4 at 32,729,996 bp
  • T to C, chromosome 4 at 45,305,785 bp
  • A to G, chromosome 4 at 97,690,718 bp
  • T to A, chromosome 4 at 113,105,909 bp
  • C to T, chromosome 5 at 72,529,169 bp
  • T to A, chromosome 5 at 81,465,341 bp
  • C to T, chromosome 5 at 88,328,266 bp
  • C to A, chromosome 5 at 90,584,310 bp
  • T to C, chromosome 5 at 137,472,191 bp
  • T to C, chromosome 6 at 84,195,263 bp
  • T to C, chromosome 6 at 120,215,057 bp
  • A to T, chromosome 7 at 42,475,559 bp
  • A to T, chromosome 7 at 83,622,361 bp
  • A to C, chromosome 7 at 103,442,940 bp
  • A to G, chromosome 7 at 131,119,511 bp
  • A to G, chromosome 8 at 13,072,578 bp
  • A to T, chromosome 8 at 104,129,362 bp
  • T to A, chromosome 10 at 120,102,901 bp
  • C to T, chromosome 11 at 32,604,166 bp
  • T to C, chromosome 11 at 46,291,586 bp
  • G to A, chromosome 11 at 51,007,343 bp
  • A to T, chromosome 11 at 58,922,318 bp
  • T to A, chromosome 11 at 62,664,998 bp
  • A to G, chromosome 11 at 73,486,176 bp
  • T to C, chromosome 11 at 115,878,391 bp
  • G to A, chromosome 11 at 119,843,713 bp
  • T to C, chromosome 12 at 87,455,715 bp
  • T to C, chromosome 12 at 103,584,151 bp
  • T to C, chromosome 12 at 118,927,326 bp
  • A to G, chromosome 13 at 13,437,910 bp
  • A to G, chromosome 13 at 54,733,250 bp
  • T to G, chromosome 13 at 63,527,245 bp
  • CG to C, chromosome 14 at 65,759,662 bp
  • C to T, chromosome 15 at 100,211,986 bp
  • A to C, chromosome 16 at 32,753,917 bp
  • G to C, chromosome 16 at 32,753,919 bp
  • A to G, chromosome 16 at 45,648,889 bp
  • A to T, chromosome 16 at 58,824,483 bp
  • A to T, chromosome 16 at 73,973,766 bp
  • G to A, chromosome 17 at 24,899,189 bp
  • A to G, chromosome 17 at 28,576,097 bp
  • A to T, chromosome 17 at 34,026,912 bp
  • A to G, chromosome 17 at 56,429,128 bp
  • T to G, chromosome 17 at 80,227,749 bp
  • T to A, chromosome 18 at 61,068,108 bp
  • T to C, chromosome 19 at 56,723,087 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5447 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
043012-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.