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Strain Name:
C57BL/6J-MtgxR5517Btlr/Mmmh
Stock Number:
043076-MU
Citation ID:
RRID:MMRRC_043076-MU
Other Names:
R5517 (G1), C57BL/6J-MtgxR5517Btlr
Major Collection:

Strain Information

Slc6a12
Name: solute carrier family 6 (neurotransmitter transporter, betaine/GABA), member 12
Synonyms: Gabt2, BGT1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 14411
Homologene: 128225
Mcm7
Name: minichromosome maintenance complex component 7
Synonyms: mCDC47, Mcmd7
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 17220
HGNC: HGNC:6950
Homologene: 4323
Spred1
Name: sprouty protein with EVH-1 domain 1, related sequence
Synonyms: Spred-1, 5730461F13Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 114715
Homologene: 24919
Chuk
Name: conserved helix-loop-helix ubiquitous kinase
Synonyms: Chuk1, IKK1, IKK[a], IKK-alpha, IkappaB kinase alpha, IKK 1, IKK alpha, IKKalpha, IKK-1
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 12675
HGNC: HGNC:1974
Homologene: 979
Dhx35
Name: DEAH-box helicase 35
Synonyms: Ddx35, 1200009D07Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 71715
Homologene: 6406
Hells
Name: helicase, lymphoid specific
Synonyms: Lysh, proliferation-associated SNF2-like, PASG, LSH, YFK8, E130115I21Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 15201
HGNC: HGNC:4861
Homologene: 50037
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 66,415,256 bp
  • T to A, chromosome 1 at 171,577,974 bp
  • T to G, chromosome 1 at 183,395,985 bp
  • T to A, chromosome 2 at 26,255,887 bp
  • G to T, chromosome 2 at 111,780,459 bp
  • G to A, chromosome 2 at 117,177,714 bp
  • G to A, chromosome 2 at 122,595,543 bp
  • G to T, chromosome 2 at 157,560,468 bp
  • T to A, chromosome 2 at 158,834,912 bp
  • T to C, chromosome 2 at 162,997,835 bp
  • C to A, chromosome 3 at 89,946,031 bp
  • T to C, chromosome 3 at 95,171,233 bp
  • C to A, chromosome 3 at 135,223,265 bp
  • T to C, chromosome 4 at 57,855,987 bp
  • G to A, chromosome 4 at 98,774,881 bp
  • A to G, chromosome 4 at 106,375,674 bp
  • T to C, chromosome 4 at 108,204,746 bp
  • T to C, chromosome 4 at 120,715,809 bp
  • T to A, chromosome 5 at 4,001,665 bp
  • A to G, chromosome 5 at 138,164,871 bp
  • A to G, chromosome 5 at 139,752,787 bp
  • A to G, chromosome 6 at 118,129,070 bp
  • A to G, chromosome 6 at 121,354,339 bp
  • A to G, chromosome 6 at 124,050,717 bp
  • T to C, chromosome 6 at 134,851,176 bp
  • A to T, chromosome 7 at 16,563,489 bp
  • C to A, chromosome 7 at 24,414,913 bp
  • T to C, chromosome 7 at 44,906,981 bp
  • A to G, chromosome 7 at 46,274,571 bp
  • C to T, chromosome 7 at 90,170,598 bp
  • C to A, chromosome 7 at 127,198,607 bp
  • T to A, chromosome 8 at 10,560,226 bp
  • T to C, chromosome 8 at 16,226,479 bp
  • A to G, chromosome 8 at 19,250,852 bp
  • G to A, chromosome 8 at 94,636,760 bp
  • T to A, chromosome 8 at 123,230,587 bp
  • G to A, chromosome 9 at 19,375,767 bp
  • T to C, chromosome 9 at 35,211,350 bp
  • T to A, chromosome 9 at 103,336,114 bp
  • T to G, chromosome 9 at 119,495,713 bp
  • A to T, chromosome 10 at 23,940,729 bp
  • A to G, chromosome 10 at 40,647,730 bp
  • A to G, chromosome 10 at 41,340,891 bp
  • T to C, chromosome 10 at 41,963,850 bp
  • A to T, chromosome 10 at 78,039,691 bp
  • A to T, chromosome 11 at 87,059,506 bp
  • A to T, chromosome 11 at 87,893,066 bp
  • T to C, chromosome 12 at 7,990,906 bp
  • C to T, chromosome 12 at 11,458,312 bp
  • A to T, chromosome 13 at 23,872,592 bp
  • T to C, chromosome 15 at 89,427,344 bp
  • C to T, chromosome 15 at 99,246,995 bp
  • C to A, chromosome 16 at 11,253,979 bp
  • T to A, chromosome 17 at 25,124,786 bp
  • G to A, chromosome 17 at 34,284,436 bp
  • C to T, chromosome 17 at 35,958,341 bp
  • C to A, chromosome 18 at 6,220,954 bp
  • G to T, chromosome 18 at 37,341,793 bp
  • C to T, chromosome 18 at 37,959,873 bp
  • G to A, chromosome 19 at 38,954,800 bp
  • T to A, chromosome 19 at 44,097,533 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5517 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043076-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.