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Strain Name:
C57BL/6J-MtgxR5665Btlr/Mmmh
Stock Number:
043308-MU
Citation ID:
RRID:MMRRC_043308-MU
Other Names:
R5665 (G1)
Major Collection:

Strain Information

Myo5a
Name: myosin VA
Synonyms: MVa, MyoVA, Myo5, flail, 9630007J19Rik, Dbv
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 17918
HGNC: HGNC:7602
Homologene: 20100
Uso1
Name: USO1 vesicle docking factor
Synonyms: transcytosis associated protein p115, TAP, Vdp
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 56041
Homologene: 2754
Pygb
Name: brain glycogen phosphorylase
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 110078
HGNC: HGNC:9723
Homologene: 100930
Gcm2
Name: glial cells missing homolog 2
Synonyms: Gcm1-rs2
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 107889
VEGA: 13
HGNC: HGNC:4198
Homologene: 3490
Spred1
Name: sprouty protein with EVH-1 domain 1, related sequence
Synonyms: Spred-1, 5730461F13Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 114715
Homologene: 24919
Slk
Name: STE20-like kinase
Synonyms: 9A2, mSLK, Etk4, SLK, Stk2
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 20874
Homologene: 22515
Elp3
Name: elongator acetyltransferase complex subunit 3
Synonyms: 2610507P14Rik, KAT9
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 74195
VEGA: 14
Homologene: 7105
Clcn6
Name: chloride channel, voltage-sensitive 6
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 26372
HGNC: HGNC:2024
Homologene: 985
Eif4g3
Name: eukaryotic translation initiation factor 4 gamma, 3
Synonyms: eIF4GII, 1500002J22Rik, 4930523M17Rik, G1-419-52, repro8
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230861
HGNC: HGNC:3298
Homologene: 2789
Plk4
Name: polo like kinase 4
Synonyms: Sak, Stk18
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 20873
Homologene: 7962
Usp15
Name: ubiquitin specific peptidase 15
Synonyms: Gcap18, 4921514G19Rik, E430033I05Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 14479
VEGA: 10
Homologene: 101542
Asap1
Name: ArfGAP with SH3 domain, ankyrin repeat and PH domain1
Synonyms: Ddef1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 13196
HGNC: HGNC:2720
Homologene: 7684
Sbno2
Name: strawberry notch 2
Synonyms: Stno
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 216161
VEGA: 10
Homologene: 8981
Scaper
Name: S phase cyclin A-associated protein in the ER
Synonyms: D530014O03Rik, Zfp291
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 244891
VEGA: 9
Homologene: 32488
Herc1
Name: HECT and RLD domain containing E3 ubiquitin protein ligase family member 1
Synonyms: D130015N03Rik, 2810449H11Rik, tbl
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235439
HGNC: HGNC:4867
Homologene: 31207
Ank3
Name: ankyrin 3, epithelial
Synonyms: Ank-3, Ankyrin-3, AnkG, 2900054D09Rik, Ankyrin-G
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 11735
HGNC: HGNC:494
Homologene: 56908
Dhx16
Name: DEAH-box helicase 16
Synonyms: 2410006N22Rik, DBP2, Ddx16, DEAH (Asp-Glu-Ala-His) box polypeptide 16
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 69192
HGNC: HGNC:2739
Homologene: 2658
Rnf114
Name: ring finger protein 114
Synonyms: Znf228, 1110008J21Rik, Zfp313
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 81018
Homologene: 10265
Cyb5r3
Name: cytochrome b5 reductase 3
Synonyms: Dia-1, 2500002N19Rik, 0610016L08Rik, Dia1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 109754
HGNC: HGNC:2873
Homologene: 47921
Med29
Name: mediator complex subunit 29
Synonyms: 2810405O22Rik, Ixl
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 67224
Homologene: 12102
Stt3b
Name: STT3, subunit of the oligosaccharyltransferase complex, homolog B (S. cerevisiae)
Synonyms: Simp, 1300006C19Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 68292
VEGA: 9
Homologene: 7387
Ahi1
Name: Abelson helper integration site 1
Synonyms: Ahi-1, 1700015F03Rik, D10Bwg0629e, Jouberin
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 52906
VEGA: 10
Homologene: 9762
Oga
Name: O-GlcNAcase
Synonyms: Hy5, 2810009A20Rik, 5830447M11Rik, 4833427O07Rik, Mgea5
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 76055
VEGA: 19
HGNC: HGNC:7056
Homologene: 8154
Plxna4
Name: plexin A4
Synonyms: Plxa4
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 243743
HGNC: HGNC:9102
Homologene: 77587
Acaca
Name: acetyl-Coenzyme A carboxylase alpha
Synonyms: acetyl-CoA carboxylase, Acc1, LOC327983, Acac, A530025K05Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 107476
HGNC: HGNC:84
Homologene: 31015
Homer1
Name: homer scaffolding protein 1
Synonyms: Ves-1, PSD-Zip45
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 26556
Homologene: 3155
Mxra8
Name: matrix-remodelling associated 8
Synonyms: 1200013A08Rik, Asp3, limitrin, DICAM
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 74761
HGNC: HGNC:7542
Homologene: 11500
Lama1
Name: laminin, alpha 1
Synonyms: Lama
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 16772
VEGA: 17
HGNC: HGNC:6481
Homologene: 21146
Srpk2
Name: serine/arginine-rich protein specific kinase 2
Synonyms: mSRPK2, WBP6
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 20817
Homologene: 101663
Zfp346
Name: zinc finger protein 346
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 26919
Homologene: 8073
Slc12a9
Name: solute carrier family 12 (potassium/chloride transporters), member 9
Synonyms: CIP1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 83704
Homologene: 5429
Zbtb10
Name: zinc finger and BTB domain containing 10
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 229055
Homologene: 11395
Vps13a
Name: vacuolar protein sorting 13A
Synonyms: 4930516E05Rik, D330038K10Rik, 4930543C13Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 271564
VEGA: 19
HGNC: HGNC:1908
Homologene: 22068
Fchsd2
Name: FCH and double SH3 domains 2
Synonyms: Sh3md3
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 207278
Homologene: 8887
Izumo1r
Name: IZUMO1 receptor, JUNO
Synonyms: Folbp3, 0910001L11Rik, Folr4, Juno
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 64931
Homologene: 11283
Dppa4
Name: developmental pluripotency associated 4
Synonyms: 2410091M23Rik, ECAT15-1
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 73693
VEGA: 16
Homologene: 130462
Syne2
Name: spectrin repeat containing, nuclear envelope 2
Synonyms: nesprin-2, syne-2, D12Ertd777e, 6820443O06Rik, Nesp2g, Cpfl8, diminished cone electroretinogram, dice
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 319565
Homologene: 56700
Pkhd1
Name: polycystic kidney and hepatic disease 1
Synonyms: tigmin, FPC
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 241035
HGNC: HGNC:9016
Homologene: 16336
Muc4
Name: mucin 4
Synonyms: 4933405I11Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 140474
HGNC: HGNC:7514
Homologene: 124469
Agbl1
Name: ATP/GTP binding protein-like 1
Synonyms: Nna1-l1, EG244071, Ccp4
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 244071
Homologene: 17552
Dpyd
Name: dihydropyrimidine dehydrogenase
Synonyms: DPD, E330028L06Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 99586
HGNC: HGNC:3012
Homologene: 85
Olfm2
Name: olfactomedin 2
Synonyms: A030009A06Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 244723
VEGA: 9
Homologene: 14380
Capn7
Name: calpain 7
Synonyms: PalBH
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 12339
HGNC: HGNC:1484
Homologene: 7651
Col6a3
Name: collagen, type VI, alpha 3
Synonyms: Col6a-3
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12835
HGNC: HGNC:2213
Homologene: 37917
Fancd2os
Name: Fancd2 opposite strand
Synonyms: 4931417G12Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 70979
Homologene: 45456
Chchd1
Name: coiled-coil-helix-coiled-coil-helix domain containing 1
Synonyms: 2400010G13Rik, 1110001O19Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 66121
VEGA: 14
Homologene: 11923
Kcnt1
Name: potassium channel, subfamily T, member 1
Synonyms: slo2, Slack, C030030G16Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227632
Homologene: 11055
Serping1
Name: serine (or cysteine) peptidase inhibitor, clade G, member 1
Synonyms: C1nh, C1 inhibitor, C1INH
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 12258
HGNC: HGNC:1228
Homologene: 44
Pigs
Name: phosphatidylinositol glycan anchor biosynthesis, class S
Synonyms: LOC245087, LOC276846
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 276846
Homologene: 41963
Ccdc13
Name: coiled-coil domain containing 13
Synonyms: 2900041A11Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 100502861
Homologene: 87213
Sntb1
Name: syntrophin, basic 1
Synonyms: beta1-Syntrophin, 59-1 DAP
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 20649
Homologene: 9618
Acp7
Name: acid phosphatase 7, tartrate resistant
Synonyms: C330005M16Rik, Papl
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 101744
Homologene: 23924
Zfp800
Name: zinc finger protein 800
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 627049
Homologene: 18529
Arhgef40
Name: Rho guanine nucleotide exchange factor 40
Synonyms: E130112L23Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 268739
Homologene: 19560
Gabrp
Name: gamma-aminobutyric acid type A receptor subunit pi
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 216643
HGNC: HGNC:4089
Homologene: 22798
Nphp4
Name: nephronophthisis 4 (juvenile) homolog (human)
Synonyms: 4930564O18Rik, nmf192
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 260305
Homologene: 9024
Stt3a
Name: STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae)
Synonyms: Itm1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 16430
HGNC: HGNC:6172
Homologene: 40617
Vmn2r24
Name: vomeronasal 2, receptor 24
Synonyms: EG243628
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 243628
Homologene: 135915
Or10x4
Name: olfactory receptor family 10 subfamily X member 4
Synonyms: GA_x6K02T2P20D-20771141-20770212, GA_x6K02T2MFC0-1145-1312, MOR267-7, Olfr415, Olfr248
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 258709
Or10ag52
Name: olfactory receptor family 10 subfamily AG member 52
Synonyms: GA_x6K02T2Q125-48697747-48698727, MOR264-25, Olfr1113
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 404327
Homologene: 27114
Cimip6
Name: ciliary microtubule inner protein 6
Synonyms: 4930505A04Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 75087
Homologene: 18988
Vmn1r189
Name: vomeronasal 1 receptor 189
Synonyms: V1rh2
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 252906
Homologene: 110880
Zbtb12
Name: zinc finger and BTB domain containing 12
Synonyms: Bat-9, Bat9
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 193736
Homologene: 18778
Myrip
Name: myosin VIIA and Rab interacting protein
Synonyms: Slac2-c, A230081N12Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 245049
Homologene: 9150
Sostdc1
Name: sclerostin domain containing 1
Synonyms: Sostl, ectodin, Wise, USAG-1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 66042
VEGA: 12
Homologene: 9154
Prl3d3
Name: prolactin family 3, subfamily d, member 3
Synonyms: PL-Ig, Plig
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 215029
Homologene: 137215
Pdpr
Name: pyruvate dehydrogenase phosphatase regulatory subunit
Synonyms: 4930402E16Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 319518
Homologene: 9948
Capn10
Name: calpain 10
Synonyms: Capn8
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 23830
HGNC: HGNC:1477
Homologene: 36323
Btbd7
Name: BTB domain containing 7
Synonyms: FUP1, 5730507E09Rik, E130118E17Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 238386
VEGA: 12
Homologene: 34300
Elovl1
Name: ELOVL fatty acid elongase 1
Synonyms: Ssc1, elongation of very long chain fatty acids (FEN1/Elo2, SUR4/Elo3, yeast)-like 1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 54325
Homologene: 41322
Gpr132
Name: G protein-coupled receptor 132
Synonyms: G2 accumulation, G2a
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 56696
VEGA: 12
Homologene: 8350
Casp7
Name: caspase 7
Synonyms: CMH-1, ICE-IAP3, Mch3, caspase-7
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 12369
VEGA: 19
HGNC: HGNC:1508
Homologene: 11168
Arl14
Name: ADP-ribosylation factor-like 14
Synonyms: 9130014L17Rik, Arf7
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 71619
Homologene: 11812
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 20,588,531 bp
  • T to C, chromosome 1 at 90,827,880 bp
  • T to A, chromosome 1 at 92,937,931 bp
  • T to C, chromosome 1 at 174,391,375 bp
  • C to T, chromosome 2 at 25,901,909 bp
  • T to C, chromosome 2 at 84,771,545 bp
  • T to A, chromosome 2 at 87,213,728 bp
  • C to T, chromosome 2 at 117,153,005 bp
  • T to C, chromosome 2 at 150,820,888 bp
  • T to C, chromosome 2 at 167,510,934 bp
  • T to C, chromosome 3 at 9,265,192 bp
  • A to G, chromosome 3 at 40,813,586 bp
  • A to C, chromosome 3 at 69,223,038 bp
  • A to G, chromosome 3 at 118,917,092 bp
  • G to T, chromosome 4 at 118,431,635 bp
  • A to G, chromosome 4 at 138,126,589 bp
  • T to A, chromosome 4 at 148,014,561 bp
  • T to C, chromosome 4 at 152,506,485 bp
  • G to T, chromosome 4 at 155,842,921 bp
  • A to G, chromosome 5 at 23,518,477 bp
  • A to T, chromosome 5 at 92,198,337 bp
  • A to G, chromosome 5 at 137,321,403 bp
  • T to C, chromosome 6 at 28,244,513 bp
  • T to C, chromosome 6 at 32,215,722 bp
  • C to A, chromosome 6 at 113,598,024 bp
  • A to G, chromosome 6 at 123,786,979 bp
  • C to T, chromosome 7 at 28,386,814 bp
  • T to A, chromosome 7 at 28,616,543 bp
  • T to A, chromosome 7 at 76,589,503 bp
  • A to G, chromosome 7 at 101,110,784 bp
  • A to G, chromosome 8 at 111,114,811 bp
  • T to C, chromosome 9 at 14,900,849 bp
  • T to G, chromosome 9 at 20,668,544 bp
  • A to G, chromosome 9 at 36,759,314 bp
  • T to C, chromosome 9 at 55,807,632 bp
  • A to G, chromosome 9 at 66,465,435 bp
  • T to C, chromosome 9 at 75,144,181 bp
  • A to T, chromosome 9 at 115,266,147 bp
  • A to G, chromosome 9 at 120,461,433 bp
  • C to A, chromosome 9 at 121,814,290 bp
  • A to G, chromosome 10 at 21,055,047 bp
  • G to A, chromosome 10 at 70,002,565 bp
  • C to T, chromosome 10 at 74,626,788 bp
  • A to G, chromosome 10 at 80,058,453 bp
  • A to T, chromosome 10 at 123,130,987 bp
  • C to T, chromosome 11 at 30,426,349 bp
  • C to G, chromosome 11 at 33,554,308 bp
  • C to A, chromosome 11 at 78,328,769 bp
  • G to T, chromosome 11 at 84,245,294 bp
  • C to A, chromosome 12 at 36,314,408 bp
  • T to A, chromosome 12 at 76,108,217 bp
  • C to A, chromosome 12 at 102,785,197 bp
  • G to A, chromosome 12 at 112,852,796 bp
  • T to C, chromosome 13 at 22,102,166 bp
  • T to A, chromosome 13 at 27,159,081 bp
  • T to A, chromosome 13 at 41,109,911 bp
  • T to C, chromosome 13 at 55,113,102 bp
  • A to T, chromosome 13 at 93,356,102 bp
  • T to C, chromosome 14 at 20,703,110 bp
  • T to C, chromosome 14 at 31,369,802 bp
  • A to G, chromosome 14 at 52,000,900 bp
  • T to C, chromosome 14 at 65,551,402 bp
  • T to A, chromosome 15 at 55,792,139 bp
  • A to G, chromosome 15 at 64,312,453 bp
  • A to G, chromosome 15 at 83,154,554 bp
  • T to C, chromosome 16 at 32,750,782 bp
  • T to C, chromosome 16 at 48,291,015 bp
  • T to C, chromosome 17 at 34,895,883 bp
  • C to T, chromosome 17 at 35,891,086 bp
  • G to T, chromosome 17 at 67,770,987 bp
  • A to T, chromosome 19 at 16,668,690 bp
  • A to C, chromosome 19 at 45,776,997 bp
  • G to A, chromosome 19 at 47,636,457 bp
  • G to A, chromosome 19 at 56,440,982 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5665 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043308-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.