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Strain Name:
C57BL/6J-MtgxR5665Btlr/Mmmh
Stock Number:
043308-MU
Citation ID:
RRID:MMRRC_043308-MU
Other Names:
R5665 (G1)
Major Collection:

Strain Information

Myo5a
Name: myosin VA
Synonyms: MVa, MyoVA, Myo5, flail, 9630007J19Rik, Dbv
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 17918
HGNC: HGNC:7602
Homologene: 20100
Uso1
Name: USO1 vesicle docking factor
Synonyms: transcytosis associated protein p115, TAP, Vdp
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 56041
Homologene: 2754
Pygb
Name: brain glycogen phosphorylase
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 110078
HGNC: HGNC:9723
Homologene: 100930
Gcm2
Name: glial cells missing homolog 2
Synonyms: Gcm1-rs2
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 107889
VEGA: 13
HGNC: HGNC:4198
Homologene: 3490
Spred1
Name: sprouty protein with EVH-1 domain 1, related sequence
Synonyms: Spred-1, 5730461F13Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 114715
Homologene: 24919
Slk
Name: STE20-like kinase
Synonyms: 9A2, mSLK, Etk4, SLK, Stk2
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 20874
Homologene: 22515
Elp3
Name: elongator acetyltransferase complex subunit 3
Synonyms: 2610507P14Rik, KAT9
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 74195
VEGA: 14
Homologene: 7105
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 20,588,531 bp
  • T to C, chromosome 1 at 90,827,880 bp
  • T to A, chromosome 1 at 92,937,931 bp
  • T to C, chromosome 1 at 174,391,375 bp
  • C to T, chromosome 2 at 25,901,909 bp
  • T to C, chromosome 2 at 84,771,545 bp
  • T to A, chromosome 2 at 87,213,728 bp
  • C to T, chromosome 2 at 117,153,005 bp
  • T to C, chromosome 2 at 150,820,888 bp
  • T to C, chromosome 2 at 167,510,934 bp
  • T to C, chromosome 3 at 9,265,192 bp
  • A to G, chromosome 3 at 40,813,586 bp
  • A to C, chromosome 3 at 69,223,038 bp
  • A to G, chromosome 3 at 118,917,092 bp
  • G to T, chromosome 4 at 118,431,635 bp
  • A to G, chromosome 4 at 138,126,589 bp
  • T to A, chromosome 4 at 148,014,561 bp
  • T to C, chromosome 4 at 152,506,485 bp
  • G to T, chromosome 4 at 155,842,921 bp
  • A to G, chromosome 5 at 23,518,477 bp
  • A to T, chromosome 5 at 92,198,337 bp
  • A to G, chromosome 5 at 137,321,403 bp
  • T to C, chromosome 6 at 28,244,513 bp
  • T to C, chromosome 6 at 32,215,722 bp
  • C to A, chromosome 6 at 113,598,024 bp
  • A to G, chromosome 6 at 123,786,979 bp
  • C to T, chromosome 7 at 28,386,814 bp
  • T to A, chromosome 7 at 28,616,543 bp
  • T to A, chromosome 7 at 76,589,503 bp
  • A to G, chromosome 7 at 101,110,784 bp
  • A to G, chromosome 8 at 111,114,811 bp
  • T to C, chromosome 9 at 14,900,849 bp
  • T to G, chromosome 9 at 20,668,544 bp
  • A to G, chromosome 9 at 36,759,314 bp
  • T to C, chromosome 9 at 55,807,632 bp
  • A to G, chromosome 9 at 66,465,435 bp
  • T to C, chromosome 9 at 75,144,181 bp
  • A to T, chromosome 9 at 115,266,147 bp
  • A to G, chromosome 9 at 120,461,433 bp
  • C to A, chromosome 9 at 121,814,290 bp
  • A to G, chromosome 10 at 21,055,047 bp
  • G to A, chromosome 10 at 70,002,565 bp
  • C to T, chromosome 10 at 74,626,788 bp
  • A to G, chromosome 10 at 80,058,453 bp
  • A to T, chromosome 10 at 123,130,987 bp
  • C to T, chromosome 11 at 30,426,349 bp
  • C to G, chromosome 11 at 33,554,308 bp
  • C to A, chromosome 11 at 78,328,769 bp
  • G to T, chromosome 11 at 84,245,294 bp
  • C to A, chromosome 12 at 36,314,408 bp
  • T to A, chromosome 12 at 76,108,217 bp
  • C to A, chromosome 12 at 102,785,197 bp
  • G to A, chromosome 12 at 112,852,796 bp
  • T to C, chromosome 13 at 22,102,166 bp
  • T to A, chromosome 13 at 27,159,081 bp
  • T to A, chromosome 13 at 41,109,911 bp
  • T to C, chromosome 13 at 55,113,102 bp
  • A to T, chromosome 13 at 93,356,102 bp
  • T to C, chromosome 14 at 20,703,110 bp
  • T to C, chromosome 14 at 31,369,802 bp
  • A to G, chromosome 14 at 52,000,900 bp
  • T to C, chromosome 14 at 65,551,402 bp
  • T to A, chromosome 15 at 55,792,139 bp
  • A to G, chromosome 15 at 64,312,453 bp
  • A to G, chromosome 15 at 83,154,554 bp
  • T to C, chromosome 16 at 32,750,782 bp
  • T to C, chromosome 16 at 48,291,015 bp
  • T to C, chromosome 17 at 34,895,883 bp
  • C to T, chromosome 17 at 35,891,086 bp
  • G to T, chromosome 17 at 67,770,987 bp
  • A to T, chromosome 19 at 16,668,690 bp
  • A to C, chromosome 19 at 45,776,997 bp
  • G to A, chromosome 19 at 47,636,457 bp
  • G to A, chromosome 19 at 56,440,982 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5665 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043308-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.