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Strain Name:
C57BL/6J-MtgxR5820Btlr/Mmmh
Stock Number:
043400-MU
Citation ID:
RRID:MMRRC_043400-MU
Other Names:
R5820 (G1)
Major Collection:

Strain Information

Tlr9
Name: toll-like receptor 9
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 81897
Homologene: 68126
Gad2
Name: glutamic acid decarboxylase 2
Synonyms: GAD65, Gad-2, 6330404F12Rik, GAD(65)
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 14417
HGNC: HGNC:4093
Homologene: 20223
Epha7
Name: Eph receptor A7
Synonyms: MDK1, Ebk, Cek11, Ehk3, Hek11, Mdk1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 13841
HGNC: HGNC:3390
Homologene: 20935
Jarid2
Name: jumonji and AT-rich interaction domain containing 2
Synonyms: Jmj, jumonji
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 16468
HGNC: HGNC:6196
Homologene: 31279
Map1b
Name: microtubule-associated protein 1B
Synonyms: MAP5, Mtap-5, Mtap5, LC1, Mtap1b
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 17755
VEGA: 13
HGNC: HGNC:6836
Homologene: 38111
Sema4b
Name: sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B
Synonyms: SemC, Semac
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 20352
Homologene: 8426
Kat8
Name: K(lysine) acetyltransferase 8
Synonyms: D7Ertd629e, 5830450F21Rik, 2010203C02Rik, MOF, Myst1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 67773
Homologene: 41676
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 98,499,778 bp
  • T to G, chromosome 1 at 107,078,359 bp
  • A to G, chromosome 1 at 136,079,604 bp
  • T to C, chromosome 1 at 166,664,601 bp
  • T to C, chromosome 1 at 183,135,587 bp
  • T to C, chromosome 2 at 22,690,249 bp
  • T to C, chromosome 2 at 91,492,615 bp
  • C to T, chromosome 2 at 144,513,488 bp
  • T to C, chromosome 3 at 37,039,526 bp
  • G to A, chromosome 3 at 96,727,848 bp
  • A to T, chromosome 3 at 133,160,799 bp
  • A to T, chromosome 4 at 28,949,365 bp
  • G to A, chromosome 4 at 41,035,510 bp
  • A to G, chromosome 4 at 62,345,546 bp
  • G to A, chromosome 5 at 73,428,632 bp
  • T to A, chromosome 5 at 110,162,739 bp
  • G to A, chromosome 5 at 114,936,978 bp
  • A to T, chromosome 5 at 135,339,920 bp
  • T to A, chromosome 6 at 49,417,285 bp
  • C to T, chromosome 6 at 82,071,173 bp
  • T to C, chromosome 7 at 30,977,152 bp
  • C to T, chromosome 7 at 49,848,806 bp
  • T to C, chromosome 7 at 80,224,958 bp
  • G to A, chromosome 7 at 104,141,467 bp
  • A to G, chromosome 7 at 105,591,788 bp
  • A to G, chromosome 7 at 116,084,458 bp
  • G to A, chromosome 7 at 127,924,816 bp
  • G to A, chromosome 8 at 3,155,976 bp
  • A to T, chromosome 8 at 61,956,121 bp
  • A to T, chromosome 8 at 95,039,593 bp
  • T to C, chromosome 9 at 38,524,599 bp
  • G to A, chromosome 9 at 77,230,482 bp
  • G to A, chromosome 9 at 106,222,707 bp
  • C to T, chromosome 9 at 124,422,765 bp
  • A to T, chromosome 10 at 7,705,908 bp
  • A to G, chromosome 10 at 7,810,410 bp
  • A to G, chromosome 10 at 42,796,245 bp
  • C to T, chromosome 10 at 79,738,323 bp
  • A to G, chromosome 10 at 99,264,002 bp
  • G to A, chromosome 10 at 107,777,117 bp
  • A to G, chromosome 11 at 32,735,374 bp
  • A to T, chromosome 11 at 46,200,704 bp
  • A to G, chromosome 11 at 50,308,383 bp
  • A to G, chromosome 11 at 69,232,696 bp
  • A to G, chromosome 11 at 102,913,048 bp
  • T to C, chromosome 11 at 104,583,980 bp
  • T to C, chromosome 12 at 34,290,632 bp
  • C to T, chromosome 12 at 100,132,287 bp
  • G to A, chromosome 13 at 22,088,086 bp
  • G to A, chromosome 13 at 44,902,301 bp
  • G to T, chromosome 13 at 93,092,780 bp
  • T to A, chromosome 13 at 99,432,824 bp
  • T to C, chromosome 14 at 51,987,496 bp
  • T to C, chromosome 14 at 54,958,680 bp
  • A to G, chromosome 14 at 118,604,195 bp
  • G to A, chromosome 15 at 76,454,841 bp
  • C to T, chromosome 15 at 78,940,480 bp
  • A to G, chromosome 15 at 91,260,697 bp
  • A to G, chromosome 17 at 4,996,254 bp
  • A to C, chromosome 17 at 25,149,052 bp
  • A to G, chromosome 17 at 46,412,773 bp
  • A to T, chromosome 17 at 66,689,465 bp
  • T to C, chromosome 18 at 34,943,174 bp
  • C to A, chromosome 18 at 35,717,267 bp
  • T to C, chromosome 18 at 42,141,047 bp
  • T to A, chromosome 18 at 56,740,786 bp
  • A to T, chromosome 18 at 84,560,524 bp
  • T to C, chromosome 19 at 6,059,422 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5820 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043400-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.