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Strain Name:
C57BL/6J-MtgxR6017Btlr/Mmmh
Stock Number:
044191-MU
Citation ID:
RRID:MMRRC_044191-MU
Other Names:
R6017 (G1)
Major Collection:

Strain Information

Ppp1r9a
Name: protein phosphatase 1, regulatory subunit 9A
Synonyms: 4930518N04Rik, neurabin-I, A230094E16Rik, 2810430P21Rik, Neurabin I, NRB
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 243725
Homologene: 14247
Cdc42ep4
Name: CDC42 effector protein 4
Synonyms: CEP4, Borg4, 1500041M20Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 56699
Homologene: 8110
Oxsr1
Name: oxidative-stress responsive 1
Synonyms: 2810422B09Rik, 2210022N24Rik, Osr1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 108737
HGNC: HGNC:8508
Homologene: 31288
Kcnj1
Name: potassium inwardly-rectifying channel, subfamily J, member 1
Synonyms: ROMK-2, ROMK, Kir1.1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 56379
VEGA: 9
HGNC: HGNC:6255
Homologene: 56764
Stk24
Name: serine/threonine kinase 24
Synonyms: 1810013H02Rik, STE20
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 223255
VEGA: 14
Homologene: 20793
Vps35l
Name: VPS35 endosomal protein sorting factor like
Synonyms: 9030624J02Rik, Vsp35l
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 71517
Homologene: 10659
Wdfy3
Name: WD repeat and FYVE domain containing 3
Synonyms: Ggtb3, 2610509D04Rik, D5Ertd66e, Bwf1, Bchs, Alfy
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 72145
Homologene: 22855
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 59,121,359 bp
  • C to A, chromosome 1 at 74,262,486 bp
  • T to A, chromosome 1 at 179,699,453 bp
  • T to C, chromosome 1 at 188,957,514 bp
  • T to G, chromosome 2 at 27,013,428 bp
  • T to A, chromosome 2 at 119,816,666 bp
  • C to A, chromosome 2 at 155,587,465 bp
  • C to A, chromosome 2 at 156,996,242 bp
  • C to T, chromosome 2 at 181,195,812 bp
  • T to A, chromosome 3 at 92,707,933 bp
  • C to A, chromosome 4 at 111,774,827 bp
  • T to A, chromosome 4 at 115,326,279 bp
  • G to T, chromosome 4 at 136,931,743 bp
  • T to C, chromosome 4 at 144,109,237 bp
  • G to T, chromosome 4 at 148,158,553 bp
  • T to C, chromosome 4 at 150,165,172 bp
  • T to C, chromosome 5 at 26,057,278 bp
  • T to C, chromosome 5 at 35,044,395 bp
  • A to T, chromosome 5 at 41,818,760 bp
  • T to C, chromosome 5 at 101,851,359 bp
  • A to G, chromosome 5 at 144,844,241 bp
  • C to A, chromosome 6 at 3,483,332 bp
  • T to A, chromosome 6 at 4,906,363 bp
  • G to A, chromosome 7 at 19,201,163 bp
  • G to A, chromosome 7 at 28,362,884 bp
  • T to C, chromosome 7 at 114,236,797 bp
  • T to A, chromosome 7 at 118,809,921 bp
  • T to A, chromosome 8 at 92,358,250 bp
  • C to A, chromosome 8 at 104,310,951 bp
  • T to C, chromosome 9 at 19,585,434 bp
  • A to T, chromosome 9 at 32,394,104 bp
  • A to G, chromosome 9 at 37,380,760 bp
  • A to T, chromosome 9 at 38,403,620 bp
  • T to C, chromosome 9 at 57,681,030 bp
  • G to A, chromosome 9 at 108,797,267 bp
  • T to C, chromosome 9 at 110,034,619 bp
  • A to G, chromosome 9 at 119,264,777 bp
  • T to C, chromosome 10 at 10,450,036 bp
  • A to T, chromosome 11 at 4,816,137 bp
  • A to G, chromosome 11 at 77,841,523 bp
  • A to T, chromosome 11 at 113,729,366 bp
  • T to C, chromosome 12 at 17,175,081 bp
  • T to C, chromosome 12 at 51,445,678 bp
  • A to G, chromosome 12 at 103,729,272 bp
  • A to G, chromosome 13 at 65,035,079 bp
  • G to A, chromosome 13 at 67,293,699 bp
  • A to T, chromosome 13 at 81,397,423 bp
  • C to T, chromosome 14 at 31,141,544 bp
  • C to T, chromosome 14 at 51,891,617 bp
  • C to T, chromosome 14 at 121,302,245 bp
  • T to A, chromosome 15 at 39,846,718 bp
  • C to A, chromosome 15 at 48,314,012 bp
  • A to G, chromosome 15 at 82,790,573 bp
  • T to C, chromosome 16 at 21,222,031 bp
  • G to T, chromosome 16 at 26,363,219 bp
  • T to C, chromosome 16 at 27,361,043 bp
  • A to G, chromosome 16 at 96,315,184 bp
  • T to A, chromosome 17 at 20,208,627 bp
  • A to G, chromosome 17 at 22,373,731 bp
  • A to C, chromosome 17 at 26,757,849 bp
  • A to T, chromosome 17 at 58,104,698 bp
  • A to G, chromosome 17 at 66,130,017 bp
  • A to G, chromosome 17 at 81,648,254 bp
  • T to C, chromosome 17 at 87,746,736 bp
  • T to C, chromosome 18 at 40,258,943 bp
  • T to A, chromosome 18 at 76,858,927 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6017 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044191-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.