Loading Mouse GIF
Loading...

Strain Name:
C57BL/6J-MtgxR6030Btlr/Mmmh
Stock Number:
044202-MU
Citation ID:
RRID:MMRRC_044202-MU
Other Names:
R6030 (G1)
Major Collection:

Strain Information

Sufu
Name: SUFU negative regulator of hedgehog signaling
Synonyms: Su(Fu), 2810026F04Rik, b2b273Clo
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 24069
Homologene: 9262
Shprh
Name: SNF2 histone linker PHD RING helicase
Synonyms: 2610103K11Rik, D230017O13Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 268281
Homologene: 6489
Crkl
Name: v-crk avian sarcoma virus CT10 oncogene homolog-like
Synonyms: Crkol, 1110025F07Rik, snoop
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 12929
HGNC: HGNC:2363
Homologene: 38021
Ptprf
Name: protein tyrosine phosphatase receptor type F
Synonyms: LAR, RPTP-LAR
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 19268
HGNC: HGNC:9670
Homologene: 20623
Agap1
Name: ArfGAP with GTPase domain, ankyrin repeat and PH domain 1
Synonyms: Ggap1, Centg2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 347722
Homologene: 56689
Col6a1
Name: collagen, type VI, alpha 1
Synonyms: Col6a-1
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 12833
HGNC: HGNC:2211
Homologene: 1391
Pck1
Name: phosphoenolpyruvate carboxykinase 1, cytosolic
Synonyms: Pck-1, PEPCK
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 18534
HGNC: HGNC:8724
Homologene: 1944
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 21,350,884 bp
  • A to G, chromosome 1 at 89,630,434 bp
  • A to G, chromosome 1 at 131,698,561 bp
  • A to G, chromosome 1 at 188,198,707 bp
  • C to A, chromosome 2 at 14,316,901 bp
  • T to C, chromosome 2 at 26,386,971 bp
  • T to G, chromosome 2 at 36,377,544 bp
  • T to C, chromosome 2 at 76,816,599 bp
  • A to T, chromosome 2 at 130,042,000 bp
  • C to A, chromosome 2 at 160,909,185 bp
  • T to C, chromosome 2 at 166,919,621 bp
  • A to G, chromosome 2 at 173,154,857 bp
  • T to C, chromosome 3 at 36,602,911 bp
  • T to C, chromosome 3 at 123,552,519 bp
  • T to A, chromosome 3 at 142,603,653 bp
  • T to C, chromosome 3 at 151,749,558 bp
  • T to C, chromosome 4 at 12,063,799 bp
  • T to C, chromosome 4 at 111,885,528 bp
  • T to C, chromosome 4 at 118,211,048 bp
  • T to A, chromosome 5 at 37,154,608 bp
  • T to C, chromosome 5 at 118,255,733 bp
  • T to C, chromosome 5 at 120,519,920 bp
  • C to T, chromosome 5 at 123,274,204 bp
  • T to C, chromosome 5 at 124,117,905 bp
  • A to T, chromosome 6 at 8,215,704 bp
  • A to G, chromosome 6 at 30,482,968 bp
  • G to T, chromosome 6 at 83,516,364 bp
  • T to A, chromosome 7 at 25,631,441 bp
  • G to C, chromosome 7 at 30,722,516 bp
  • T to A, chromosome 7 at 51,574,825 bp
  • GGCG to GGCGACGGCTGCG, chromosome 7 at 97,579,906 bp
  • C to A, chromosome 7 at 102,542,610 bp
  • A to G, chromosome 7 at 107,903,413 bp
  • A to C, chromosome 7 at 108,646,226 bp
  • A to G, chromosome 7 at 120,533,798 bp
  • G to C, chromosome 8 at 39,071,406 bp
  • T to A, chromosome 8 at 71,458,150 bp
  • A to G, chromosome 8 at 117,043,237 bp
  • G to A, chromosome 9 at 35,561,184 bp
  • T to C, chromosome 9 at 39,623,341 bp
  • C to T, chromosome 9 at 44,263,760 bp
  • C to T, chromosome 9 at 54,393,673 bp
  • T to A, chromosome 9 at 105,204,888 bp
  • A to T, chromosome 10 at 4,746,622 bp
  • A to C, chromosome 10 at 11,151,991 bp
  • C to T, chromosome 10 at 43,524,256 bp
  • T to C, chromosome 10 at 76,709,866 bp
  • T to A, chromosome 10 at 106,906,477 bp
  • A to T, chromosome 10 at 129,322,369 bp
  • G to T, chromosome 11 at 55,310,303 bp
  • T to C, chromosome 11 at 70,254,591 bp
  • A to G, chromosome 11 at 72,045,750 bp
  • A to G, chromosome 11 at 77,121,262 bp
  • A to C, chromosome 11 at 97,052,261 bp
  • A to T, chromosome 11 at 97,677,225 bp
  • C to T, chromosome 11 at 118,025,549 bp
  • T to A, chromosome 11 at 120,720,648 bp
  • A to C, chromosome 12 at 86,063,850 bp
  • A to G, chromosome 12 at 101,058,400 bp
  • C to T, chromosome 13 at 21,312,340 bp
  • T to A, chromosome 13 at 51,139,516 bp
  • A to G, chromosome 13 at 74,695,937 bp
  • T to C, chromosome 14 at 30,956,933 bp
  • T to A, chromosome 14 at 31,268,027 bp
  • T to A, chromosome 14 at 55,832,440 bp
  • T to C, chromosome 15 at 82,177,276 bp
  • A to G, chromosome 16 at 17,084,513 bp
  • A to G, chromosome 16 at 17,452,740 bp
  • T to A, chromosome 16 at 32,499,042 bp
  • A to G, chromosome 16 at 33,962,138 bp
  • G to A, chromosome 17 at 15,401,910 bp
  • A to T, chromosome 18 at 44,276,082 bp
  • G to A, chromosome 18 at 61,307,934 bp
  • T to C, chromosome 18 at 67,318,428 bp
  • T to G, chromosome 18 at 78,857,711 bp
  • C to T, chromosome 19 at 6,388,812 bp
  • T to C, chromosome 19 at 46,475,539 bp
  • A to G, chromosome 19 at 61,296,973 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6030 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044202-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.