MMRRC:044394-MU

TTGGTGCTGCTGGTGCTGCTGGTGCTGCTGGTGCTGCTGGTGCTGCTGGTGCTGTTGGTGCTGCTGGTGCTGCTGGTGCTGCTGGTGCTGCTGGTGCTGCTGGTGCTGTTGGTGCTGCTGGTGCTGCTG to TTGGTGCTGCTGGTGCTGCTGGTGCTGCTGGTGCTGCTGGTGCTGTTGGTGCTGCTGGTGCTGCTGGTGCTGCTGGTGCTGCTGGTGCTGCTGGTGCTGTTGGTGCTGCTGGTGCTGCTG, chromosome 1 at 88,503,344 bp
G to A, chromosome 1 at 134,622,098 bp
A to T, chromosome 2 at 70,126,985 bp
A to T, chromosome 2 at 76,814,371 bp
G to A, chromosome 2 at 82,990,918 bp
A to T, chromosome 2 at 89,002,196 bp
A to T, chromosome 2 at 121,473,890 bp
T to C, chromosome 2 at 127,186,953 bp
A to G, chromosome 2 at 145,928,212 bp
T to A, chromosome 2 at 151,587,137 bp
A to T, chromosome 2 at 154,564,448 bp
G to A, chromosome 2 at 157,228,656 bp
G to A, chromosome 2 at 157,817,832 bp
T to A, chromosome 2 at 173,771,543 bp
G to A, chromosome 2 at 178,033,733 bp
A to G, chromosome 3 at 101,018,810 bp
G to A, chromosome 3 at 123,117,682 bp
A to G, chromosome 3 at 126,953,226 bp
A to G, chromosome 3 at 142,734,606 bp
A to G, chromosome 3 at 144,847,171 bp
A to G, chromosome 3 at 152,384,187 bp
A to G, chromosome 4 at 46,135,542 bp
T to C, chromosome 4 at 91,260,818 bp
T to C, chromosome 4 at 111,799,118 bp
G to A, chromosome 4 at 117,123,760 bp
G to T, chromosome 4 at 129,619,445 bp
GCC to G, chromosome 4 at 153,960,953 bp
A to T, chromosome 5 at 25,294,734 bp
A to G, chromosome 5 at 35,280,873 bp
A to G, chromosome 5 at 65,638,577 bp
G to T, chromosome 5 at 89,046,217 bp
A to T, chromosome 5 at 105,280,089 bp
A to T, chromosome 5 at 106,969,297 bp
G to A, chromosome 5 at 114,623,614 bp
A to T, chromosome 5 at 123,558,630 bp
C to T, chromosome 6 at 29,859,698 bp
G to A, chromosome 6 at 34,412,476 bp
T to A, chromosome 6 at 112,380,996 bp
T to C, chromosome 7 at 28,140,183 bp
T to A, chromosome 7 at 47,053,046 bp
T to C, chromosome 7 at 81,333,422 bp
C to T, chromosome 7 at 101,898,603 bp
T to C, chromosome 7 at 104,084,891 bp
A to T, chromosome 7 at 105,713,684 bp
A to G, chromosome 7 at 121,667,782 bp
G to T, chromosome 8 at 34,817,304 bp
T to A, chromosome 8 at 111,619,194 bp
C to A, chromosome 8 at 123,509,843 bp
A to G, chromosome 9 at 41,043,473 bp
A to G, chromosome 9 at 96,687,643 bp
C to T, chromosome 9 at 108,828,582 bp
G to A, chromosome 10 at 7,866,326 bp
C to A, chromosome 10 at 83,191,249 bp
T to C, chromosome 10 at 89,112,840 bp
G to A, chromosome 10 at 99,445,147 bp
T to C, chromosome 10 at 127,350,601 bp
CGGAGGAGGAGGAGGAGGAGGAGG to CGGAGGAGGAGGAGGAGGAGG, chromosome 11 at 3,977,719 bp
C to T, chromosome 11 at 51,048,470 bp
T to A, chromosome 11 at 69,794,153 bp
AGCGGTCGTAGGC to AGC, chromosome 11 at 73,395,654 bp
T to A, chromosome 11 at 78,049,488 bp
A to G, chromosome 11 at 82,890,370 bp
A to T, chromosome 11 at 83,856,037 bp
T to A, chromosome 11 at 100,880,583 bp
A to T, chromosome 11 at 101,775,341 bp
C to T, chromosome 11 at 118,127,282 bp
A to T, chromosome 12 at 8,791,743 bp
G to C, chromosome 12 at 81,377,830 bp
A to T, chromosome 12 at 101,594,284 bp
A to G, chromosome 13 at 54,736,456 bp
G to A, chromosome 13 at 73,639,243 bp
A to G, chromosome 13 at 103,857,409 bp
T to C, chromosome 14 at 28,522,770 bp
A to T, chromosome 14 at 63,036,110 bp
G to A, chromosome 15 at 35,886,948 bp
C to G, chromosome 15 at 55,642,795 bp
A to T, chromosome 15 at 76,455,289 bp
T to C, chromosome 16 at 21,219,105 bp
T to C, chromosome 17 at 45,669,962 bp
T to A, chromosome 17 at 53,836,911 bp
G to T, chromosome 17 at 56,271,117 bp
T to A, chromosome 18 at 36,993,734 bp
G to T, chromosome 18 at 37,515,719 bp
T to A, chromosome 18 at 42,216,959 bp
T to C, chromosome 19 at 9,007,175 bp
T to C, chromosome 19 at 28,927,689 bp
T to C, chromosome 19 at 46,395,664 bp
T to C, chromosome 19 at 60,834,836 bp
A to G, chromosome Y at 1,265,965 bp

Genotype Determination

Genotyping Protocol(s)
Center protocol and contact for technical support will be shipped with mice.

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.

Strain Development

C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.

Suggested Control Mice

Wild-type littermates or C57BL/6J mice

MMRRC Genetic QC

MMRRC Genetic QC Summary

The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.

Strain Origin

Donor

Bruce Beutler, M.D., University of Texas Southwestern Medical Center.

Primary Reference

MUTAGENETIX record for R5396 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information Expand All

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat Color

Black

Breeding

MMRRC Breeding System

Random intra-strain mating

Breeding Scheme(s)

When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.

Overall Breeding Performance

Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain

Reproductive Statistics

Average litter size

Undetermined

Recommended wean age

3 weeks

Order Request Information Expand All

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)

MMRRC Item #	Description	Distribution Fee / Unit (US $) Shipping & Handling not included	Units	Notes
044394-MU-RESUS	Litter recovered from cryo-archive	$5,475.00 / Non-Profit	Litter	Recovered litter⁴; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

¹ The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

² An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

³ An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

⁴ The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.

Strain Detail Sheet

Strain Information Expand All

Colony and Husbandry Information Expand All

Order Request Information Expand All