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Strain Name:
C57BL/6J-MtgxR6429Btlr/Mmmh
Stock Number:
044567-MU
Citation ID:
RRID:MMRRC_044567-MU
Other Names:
R6429 (G1)
Major Collection:

Strain Information

Zmpste24
Name: zinc metallopeptidase, STE24
Synonyms: A530043O15Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230709
Homologene: 4277
Wrn
Name: Werner syndrome RecQ like helicase
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 22427
Homologene: 6659
Il4
Name: interleukin 4
Synonyms: Il-4
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 16189
HGNC: HGNC:6014
Homologene: 491
Macf1
Name: microtubule-actin crosslinking factor 1
Synonyms: Acf7, Aclp7, trabeculin alpha
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11426
Homologene: 136191
Cemip2
Name: cell migration inducing hyaluronidase 2
Synonyms: 3110012M15Rik, Tmem2
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 83921
VEGA: 19
Homologene: 75008
Lrp2
Name: low density lipoprotein receptor-related protein 2
Synonyms: Megalin, Gp330, D230004K18Rik, b2b1625.2Clo
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 14725
HGNC: HGNC:6694
Homologene: 20952
Fryl
Name: FRY like transcription coactivator
Synonyms: 2310004H21Rik, 2510002A14Rik, 9030227G01Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 72313
Homologene: 103956
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 123,367,601 bp
  • C to T, chromosome 1 at 132,449,804 bp
  • A to C, chromosome 2 at 66,526,963 bp
  • C to T, chromosome 2 at 69,461,287 bp
  • G to A, chromosome 2 at 88,831,525 bp
  • T to C, chromosome 3 at 113,569,509 bp
  • C to A, chromosome 4 at 33,320,606 bp
  • A to G, chromosome 4 at 121,095,670 bp
  • A to T, chromosome 4 at 123,401,594 bp
  • T to A, chromosome 4 at 135,557,196 bp
  • C to T, chromosome 5 at 73,090,751 bp
  • C to T, chromosome 5 at 108,653,708 bp
  • A to T, chromosome 5 at 110,078,659 bp
  • G to A, chromosome 5 at 114,228,591 bp
  • T to A, chromosome 5 at 119,674,191 bp
  • A to G, chromosome 5 at 138,342,050 bp
  • T to A, chromosome 6 at 38,398,313 bp
  • T to A, chromosome 6 at 131,310,064 bp
  • A to T, chromosome 7 at 3,822,346 bp
  • A to T, chromosome 7 at 64,268,504 bp
  • T to A, chromosome 7 at 85,559,068 bp
  • A to G, chromosome 7 at 100,432,091 bp
  • T to C, chromosome 7 at 105,556,928 bp
  • T to C, chromosome 7 at 127,385,042 bp
  • T to A, chromosome 7 at 143,018,742 bp
  • G to A, chromosome 8 at 33,342,996 bp
  • G to A, chromosome 8 at 39,615,817 bp
  • T to C, chromosome 9 at 37,661,165 bp
  • A to G, chromosome 9 at 39,600,614 bp
  • A to G, chromosome 11 at 53,613,909 bp
  • A to G, chromosome 11 at 54,515,567 bp
  • G to T, chromosome 11 at 68,947,796 bp
  • T to C, chromosome 11 at 98,772,014 bp
  • G to T, chromosome 11 at 119,544,721 bp
  • A to T, chromosome 12 at 73,103,473 bp
  • C to T, chromosome 12 at 112,604,256 bp
  • T to G, chromosome 13 at 37,932,129 bp
  • T to A, chromosome 14 at 30,773,911 bp
  • T to C, chromosome 15 at 5,242,997 bp
  • A to G, chromosome 15 at 98,814,523 bp
  • A to G, chromosome 15 at 101,922,794 bp
  • T to A, chromosome 16 at 34,332,164 bp
  • A to T, chromosome 16 at 90,762,430 bp
  • T to A, chromosome 16 at 91,658,166 bp
  • A to G, chromosome 17 at 35,024,199 bp
  • A to G, chromosome 17 at 45,119,143 bp
  • C to A, chromosome 17 at 71,310,956 bp
  • T to C, chromosome 18 at 3,421,345 bp
  • T to A, chromosome 18 at 5,770,498 bp
  • T to A, chromosome 18 at 7,920,163 bp
  • C to T, chromosome 19 at 4,426,105 bp
  • T to A, chromosome 19 at 4,841,235 bp
  • T to C, chromosome 19 at 21,801,908 bp
  • T to C, chromosome 19 at 29,952,000 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6429 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044567-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.