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Strain Name:
C57BL/6J-MtgxR6734Btlr/Mmmh
Stock Number:
044852-MU
Citation ID:
RRID:MMRRC_044852-MU
Other Names:
R6734 (G1)
Major Collection:

Strain Information

Arhgef10
Name: Rho guanine nucleotide exchange factor 10
Synonyms: 6430549H08Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 234094
Homologene: 22827
Pdzd2
Name: PDZ domain containing 2
Synonyms: 4930537L06Rik, LOC223364, A930022H17Rik, Pdzk3, Gm21706
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 68070
Homologene: 23393
Stk11ip
Name: serine/threonine kinase 11 interacting protein
Synonyms: LKB1IP, LIP1, 1200014D22Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 71728
Homologene: 12406
Cnot2
Name: CCR4-NOT transcription complex, subunit 2
Synonyms: 2600016M12Rik, 2810470K03Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 72068
HGNC: HGNC:7878
Homologene: 40953
Prex2
Name: phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 2
Synonyms: 6230420N16Rik, C030045D06Rik, Depdc2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 109294
Homologene: 23523
Ano6
Name: anoctamin 6
Synonyms: 2900059G15Rik, F730003B03Rik, Tmem16f
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 105722
VEGA: 15
Homologene: 27888
Sec62
Name: SEC62 homolog, preprotein translocation
Synonyms: HTP1, Dtrp1, SEC62, 3100002M17Rik, Tloc1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 69276
Homologene: 2449
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 11,080,059 bp
  • T to C, chromosome 1 at 75,532,369 bp
  • G to A, chromosome 2 at 91,485,897 bp
  • A to T, chromosome 2 at 126,126,976 bp
  • C to A, chromosome 2 at 150,945,083 bp
  • T to G, chromosome 2 at 160,781,720 bp
  • A to G, chromosome 3 at 30,810,460 bp
  • C to A, chromosome 3 at 145,508,674 bp
  • C to T, chromosome 4 at 106,760,130 bp
  • C to A, chromosome 4 at 128,463,813 bp
  • T to C, chromosome 4 at 155,860,800 bp
  • A to C, chromosome 5 at 3,850,226 bp
  • A to G, chromosome 5 at 45,733,497 bp
  • G to A, chromosome 5 at 110,620,599 bp
  • C to T, chromosome 5 at 121,438,825 bp
  • T to C, chromosome 6 at 41,445,387 bp
  • TG to TGG, chromosome 6 at 108,664,857 bp
  • C to A, chromosome 6 at 113,334,384 bp
  • T to A, chromosome 7 at 19,200,507 bp
  • G to T, chromosome 7 at 44,353,686 bp
  • G to C, chromosome 7 at 80,336,998 bp
  • T to C, chromosome 7 at 144,479,053 bp
  • A to T, chromosome 8 at 14,975,053 bp
  • A to T, chromosome 8 at 45,381,441 bp
  • A to T, chromosome 9 at 109,108,920 bp
  • G to A, chromosome 10 at 116,498,153 bp
  • G to A, chromosome 10 at 119,211,992 bp
  • A to G, chromosome 10 at 130,210,257 bp
  • A to G, chromosome 11 at 98,649,590 bp
  • T to C, chromosome 12 at 102,219,000 bp
  • T to A, chromosome 13 at 21,368,796 bp
  • T to G, chromosome 13 at 59,608,191 bp
  • G to T, chromosome 14 at 26,608,619 bp
  • T to C, chromosome 15 at 12,592,465 bp
  • T to C, chromosome 15 at 71,462,780 bp
  • C to T, chromosome 15 at 76,194,403 bp
  • A to G, chromosome 15 at 95,949,536 bp
  • C to T, chromosome 15 at 99,314,903 bp
  • G to T, chromosome 15 at 102,103,116 bp
  • T to C, chromosome 16 at 20,107,529 bp
  • A to G, chromosome 16 at 21,983,957 bp
  • A to G, chromosome 16 at 56,034,126 bp
  • T to C, chromosome 17 at 23,900,571 bp
  • T to G, chromosome 17 at 32,055,304 bp
  • G to A, chromosome 18 at 47,279,169 bp
  • T to C, chromosome 18 at 58,035,960 bp
  • G to A, chromosome 18 at 74,276,043 bp
  • T to C, chromosome 19 at 4,903,896 bp
  • T to C, chromosome 19 at 7,096,159 bp
  • T to G, chromosome 19 at 17,003,733 bp
  • T to A, chromosome 19 at 37,701,212 bp
  • T to C, chromosome 19 at 56,345,509 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6734 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044852-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.