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Strain Name:
C57BL/6J-MtgxR6942Btlr/Mmmh
Stock Number:
045056-MU
Citation ID:
RRID:MMRRC_045056-MU
Other Names:
R6942 (G1)
Major Collection:

Strain Information

Cacna1h
Name: calcium channel, voltage-dependent, T type, alpha 1H subunit
Synonyms: alpha13.2, Cav3.2, T-type Cav3.2
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 58226
HGNC: HGNC:1395
Homologene: 56913
Dlc1
Name: deleted in liver cancer 1
Synonyms: p122-RhoGAP, STARD12, Arhgap7, A730069N07Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 50768
HGNC: HGNC:2897
Homologene: 4442
Crybg3
Name: beta-gamma crystallin domain containing 3
Synonyms: Gm9581
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 224273
Homologene: 28544
Cyp11b2
Name: cytochrome P450, family 11, subfamily b, polypeptide 2
Synonyms: aldosterone synthase, Cyp11b-2, Cyp11b, steroid-11-beta-hydroxylase
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 13072
Homologene: 128035
Med13l
Name: mediator complex subunit 13-like
Synonyms: 2210413I17Rik, 6330591G05Rik, Trap240L, 9030618F05Rik, Thrap2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 76199
Homologene: 25256
Sipa1l3
Name: signal-induced proliferation-associated 1 like 3
Synonyms: 2610511M17Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 74206
Homologene: 77938
Mtbp
Name: Mdm2, transformed 3T3 cell double minute p53 binding protein
Synonyms: MDM2BP
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 105837
HGNC: HGNC:7417
Homologene: 11102
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 16,677,156 bp
  • T to A, chromosome 1 at 74,518,995 bp
  • A to G, chromosome 2 at 76,901,846 bp
  • C to T, chromosome 2 at 92,370,822 bp
  • A to G, chromosome 2 at 119,383,502 bp
  • T to A, chromosome 3 at 37,643,044 bp
  • G to T, chromosome 3 at 95,173,208 bp
  • G to A, chromosome 4 at 53,735,128 bp
  • A to T, chromosome 4 at 96,112,864 bp
  • A to G, chromosome 4 at 117,166,378 bp
  • A to T, chromosome 4 at 129,651,721 bp
  • G to A, chromosome 5 at 27,469,459 bp
  • T to C, chromosome 5 at 114,191,963 bp
  • C to A, chromosome 5 at 118,745,006 bp
  • T to A, chromosome 5 at 144,784,043 bp
  • T to A, chromosome 6 at 89,870,337 bp
  • C to T, chromosome 6 at 121,473,103 bp
  • T to C, chromosome 7 at 29,386,091 bp
  • A to G, chromosome 7 at 33,172,139 bp
  • C to T, chromosome 7 at 104,938,897 bp
  • T to C, chromosome 8 at 36,938,210 bp
  • C to T, chromosome 8 at 70,291,551 bp
  • G to T, chromosome 8 at 116,956,713 bp
  • T to A, chromosome 8 at 124,545,136 bp
  • G to A, chromosome 9 at 65,120,268 bp
  • C to T, chromosome 9 at 110,728,016 bp
  • G to A, chromosome 9 at 121,788,180 bp
  • A to T, chromosome 10 at 22,067,261 bp
  • G to A, chromosome 10 at 60,438,856 bp
  • T to A, chromosome 10 at 79,976,119 bp
  • A to T, chromosome 10 at 127,651,262 bp
  • A to T, chromosome 11 at 67,660,151 bp
  • A to G, chromosome 11 at 80,155,065 bp
  • T to A, chromosome 12 at 103,122,445 bp
  • A to G, chromosome 12 at 111,592,654 bp
  • A to T, chromosome 13 at 33,494,905 bp
  • A to T, chromosome 13 at 92,516,631 bp
  • T to C, chromosome 14 at 21,775,153 bp
  • A to G, chromosome 14 at 50,836,737 bp
  • A to G, chromosome 15 at 44,522,629 bp
  • T to A, chromosome 15 at 55,567,200 bp
  • G to A, chromosome 15 at 74,856,245 bp
  • T to C, chromosome 16 at 56,825,782 bp
  • C to T, chromosome 16 at 59,539,820 bp
  • G to T, chromosome 17 at 25,385,039 bp
  • A to G, chromosome 17 at 81,408,120 bp
  • T to C, chromosome 18 at 37,732,643 bp
  • A to C, chromosome 18 at 84,730,424 bp
  • T to A, chromosome 19 at 6,346,709 bp
  • A to T, chromosome 19 at 25,424,173 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6942 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045056-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.