Strain Name:
C57BL/6J-MtgxR6993Btlr/Mmmh
Stock Number:
045099-MU
Citation ID:
RRID:MMRRC_045099-MU
Other Names:
R6993 (G1)
Major Collection:

Strain Information

Camk2a
Name: calcium/calmodulin-dependent protein kinase II alpha
Synonyms: alpha-CaMKII
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 12322
HGNC: HGNC:1460
Homologene: 56577
Kat2b
Name: K(lysine) acetyltransferase 2B
Synonyms: A930006P13Rik, Pcaf
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 18519
HGNC: HGNC:8638
Homologene: 20834
Ewsr1
Name: Ewing sarcoma breakpoint region 1
Synonyms: Ews
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 14030
HGNC: HGNC:3508
Homologene: 134632
Tbcel
Name: tubulin folding cofactor E-like
Synonyms: E130107N23Rik, Lrrc35
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 272589
Homologene: 16120
Mast4
Name: microtubule associated serine/threonine kinase family member 4
Synonyms: 4930420O11Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 328329
Homologene: 42094
Celf1
Name: CUGBP, Elav-like family member 1
Synonyms: CUG-BP, Brunol2, D2Wsu101e, 1600010O03Rik, CUG-BP1, Cugbp1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 13046
HGNC: HGNC:2549
Homologene: 134102
Sec16a
Name: SEC16 homolog A, endoplasmic reticulum export factor
Synonyms: C230052J16Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227648
Homologene: 10533
Myo18a
Name: myosin XVIIIA
Synonyms: MyoPDZ
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 360013
Homologene: 7977
Braf
Name: Braf transforming gene
Synonyms: Braf-2, Braf2, 9930012E13Rik, D6Ertd631e
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 109880
HGNC: HGNC:1097
Homologene: 3197
Ppp1r12a
Name: protein phosphatase 1, regulatory subunit 12A
Synonyms: 1200015F06Rik, Mypt1, D10Ertd625e, 5730577I22Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 17931
VEGA: 10
HGNC: HGNC:7618
Homologene: 1855
Akap9
Name: A kinase anchor protein 9
Synonyms: AKAP450, 5730481H23Rik, G1-448-15, repro12, mei2-5
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 100986
HGNC: HGNC:379
Homologene: 134583
F2rl2
Name: coagulation factor II thrombin receptor like 2
Synonyms: PAR3, PAR-3
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 14064
HGNC: HGNC:3539
Homologene: 36151
Slc19a2
Name: solute carrier family 19 (thiamine transporter), member 2
Synonyms: DDA1, TRMA, THTR1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 116914
Homologene: 38258
Ptcd3
Name: pentatricopeptide repeat domain 3
Synonyms: 2610034F17Rik, 2810422B04Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 69956
Homologene: 41211
Wwc2
Name: WW, C2 and coiled-coil domain containing 2
Synonyms: D8Ertd594e
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 52357
Homologene: 32618
Ric1
Name: RAB6A GEF complex partner 1
Synonyms: C130057E09Rik, C030046E11Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 226089
Homologene: 13806
Unc45a
Name: unc-45 myosin chaperone A
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 101869
Homologene: 32423
Cryab
Name: crystallin, alpha B
Synonyms: alpha B-crystallin, Crya-2, Crya2, HspB5
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 12955
VEGA: 9
HGNC: HGNC:2389
Homologene: 68209
Fbf1
Name: Fas binding factor 1
Synonyms: 1110033G01Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 217335
Homologene: 16531
Sppl3
Name: signal peptide peptidase 3
Synonyms: 4833416I09Rik, Usmg3
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 74585
Homologene: 15563
Drg1
Name: developmentally regulated GTP binding protein 1
Synonyms: Nedd3
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 13494
HGNC: HGNC:3029
Homologene: 3060
Tenm3
Name: teneurin transmembrane protein 3
Synonyms: Ten-m3, 2610100B16Rik, Odz3
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 23965
Homologene: 22673
Rmnd5b
Name: required for meiotic nuclear division 5 homolog B
Synonyms: 0610039K22Rik, Gid2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 66089
Homologene: 100777
Golm1
Name: golgi membrane protein 1
Synonyms: D030064E01Rik, PSEC0257, GP73, 2310001L02Rik, Golph2
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 105348
VEGA: 13
Homologene: 12346
Pdcd2
Name: programmed cell death 2
Synonyms: RP-8
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 18567
HGNC: HGNC:8762
Homologene: 1951
Cntn3
Name: contactin 3
Synonyms: Pang
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 18488
HGNC: HGNC:2173
Homologene: 7461
Fat3
Name: FAT atypical cadherin 3
Synonyms: LOC234973, LOC382129, D430038H04Rik, 9430076A06Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 270120
VEGA: 9
Homologene: 82252
Tesk1
Name: testis specific protein kinase 1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 21754
Homologene: 4577
Unc80
Name: unc-80, NALCN activator
Synonyms: C230061B10Rik, C030018G13Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 329178
Homologene: 122243
Insr
Name: insulin receptor
Synonyms: IR, CD220, 4932439J01Rik, D630014A15Rik, IR-B, IR-A
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 16337
HGNC: HGNC:6091
Homologene: 20090
Hivep1
Name: human immunodeficiency virus type I enhancer binding protein 1
Synonyms: Cryabp1, alphaA-CRYBP1
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 110521
VEGA: 13
HGNC: HGNC:4920
Homologene: 1596
Zfp947
Name: zinc finger protein 947
Synonyms: Gm4769
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 210853
Homologene: 133253
Or10d5
Name: olfactory receptor family 10 subfamily D member 5
Synonyms: GA_x6K02T2PVTD-33651220-33650288, MOR224-2, Olfr975
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 258825
VEGA: 9
Homologene: 27275
Dnah7b
Name: dynein, axonemal, heavy chain 7B
Synonyms: LOC227058, Dnahc7b
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 227058
Homologene: 41287
Kdr
Name: kinase insert domain protein receptor
Synonyms: VEGF receptor-2, VEGFR-2, vascular endothelial growth factor receptor- 2, VEGFR2, Flk-1, Flk1, orv
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 16542
HGNC: HGNC:6307
Homologene: 55639
C5ar1
Name: complement component 5a receptor 1
Synonyms: C5aR, Cd88, C5r1, D7Msu1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 12273
HGNC: HGNC:1338
Homologene: 20413
Krt2
Name: keratin 2
Synonyms: Krt2-2, Krt2e, Krt2-17
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 16681
HGNC: HGNC:6439
Ctsf
Name: cathepsin F
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 56464
VEGA: 19
HGNC: HGNC:2531
Homologene: 31194
Irf9
Name: interferon regulatory factor 9
Synonyms: p48, Irf-9, Isgf3g
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 16391
HGNC: HGNC:6131
Homologene: 4436
Fndc7
Name: fibronectin type III domain containing 7
Synonyms: E230011A21Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 320181
Homologene: 18551
Vmn2r112
Name: vomeronasal 2, receptor 112
Synonyms: EG628185
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 628185
Homologene: 86604
Lvrn
Name: laeverin
Synonyms: 4833403I15Rik, Aqpep
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 74574
VEGA: 18
Homologene: 51352
Etfdh
Name: electron transferring flavoprotein, dehydrogenase
Synonyms: 0610010I20Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 66841
HGNC: HGNC:3483
Homologene: 3275
1700011L22Rik
Name: RIKEN cDNA 1700011L22 gene
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 67687
Homologene: 78034
Lrrc27
Name: leucine rich repeat containing 27
Synonyms: 1700071K18Rik, 2310044E02Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 76612
Homologene: 19268
H2-DMb1
Name: histocompatibility 2, class II, locus Mb1
Synonyms: H2-Mb1, H-2Mb1, H2-M beta1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 14999
HGNC: HGNC:4935
Homologene: 68231
Tbx5
Name: T-box 5
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 21388
Homologene: 160
Cd163
Name: CD163 antigen
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 93671
HGNC: HGNC:1631
Homologene: 128811
Fastkd5
Name: FAST kinase domains 5
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 380601
Homologene: 36400
Etfb
Name: electron transferring flavoprotein, beta polypeptide
Synonyms: 2810441H06Rik, 0610009I16Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 110826
HGNC: HGNC:3482
Homologene: 1503
Ptx4
Name: pentraxin 4
Synonyms: 1110018H23Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 68509
VEGA: 17
Homologene: 19179
Slc16a11
Name: solute carrier family 16 (monocarboxylic acid transporters), member 11
Synonyms: MNCb-2717
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 216867
Homologene: 27409
Slco1a7
Name: solute carrier organic anion transporter family, member 1a7
Synonyms: Gm5724
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 435927
Ldc1
Name: leucine decarboxylase 1
Synonyms: LOC332942, Gm853
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 332942
Homologene: 128653
Ctsw
Name: cathepsin W
Synonyms: lymphopain
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 13041
VEGA: 19
HGNC: HGNC:2546
Homologene: 37471
Gfi1
Name: growth factor independent 1 transcription repressor
Synonyms: Pal-1, Pal1, Gfi-1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 14581
HGNC: HGNC:4237
Homologene: 3854
H2-T3
Name: histocompatibility 2, T region locus 3
Synonyms: H-2T3, TL, H2-Tw3
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 15043
Homologene: 74884
Gm5591
Name: predicted gene 5591
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 434171
Homologene: 80005
Or52ab2
Name: olfactory receptor family 52 subfamily AB member 2
Synonyms: GA_x6K02T2PBJ9-6029614-6030561, MOR23-2, Olfr597
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 258135
Homologene: 132401
Psmb8
Name: proteasome (prosome, macropain) subunit, beta type 8 (large multifunctional peptidase 7)
Synonyms: Lmp-7, Lmp7
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 16913
HGNC: HGNC:9545
Homologene: 56499
Krtap4-6
Name: keratin associated protein 4-6
Synonyms: 1110054P19Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 68768
Homologene: 124486
Malrd1
Name: MAM and LDL receptor class A domain containing 1
Synonyms: Gm13318, Gm13364, Diet1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 102635496
Homologene: 136214
Fam162a
Name: family with sequence similarity 162, member A
Synonyms: 2310056P07Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 70186
VEGA: 16
Homologene: 8656
Hes3
Name: hes family bHLH transcription factor 3
Synonyms: bHLHb43
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 15207
Homologene: 7358
Pcdhga10
Name: protocadherin gamma subfamily A, 10
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 93722
HGNC: HGNC:8697
Homologene: 49567
Slc2a6
Name: solute carrier family 2 (facilitated glucose transporter), member 6
Synonyms: Glut6, F630103L12Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227659
Homologene: 69236
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 46,195,139 bp
  • A to T, chromosome 1 at 66,549,793 bp
  • T to A, chromosome 1 at 164,260,822 bp
  • A to T, chromosome 2 at 16,150,791 bp
  • A to T, chromosome 2 at 26,423,574 bp
  • G to T, chromosome 2 at 27,027,243 bp
  • T to C, chromosome 2 at 91,010,476 bp
  • A to G, chromosome 2 at 130,616,539 bp
  • A to G, chromosome 3 at 79,612,031 bp
  • A to G, chromosome 3 at 108,876,591 bp
  • A to G, chromosome 4 at 43,447,006 bp
  • A to G, chromosome 4 at 130,218,313 bp
  • T to C, chromosome 4 at 152,286,923 bp
  • A to G, chromosome 5 at 4,065,866 bp
  • T to C, chromosome 5 at 75,972,411 bp
  • T to A, chromosome 5 at 107,717,768 bp
  • T to A, chromosome 5 at 115,082,290 bp
  • A to T, chromosome 5 at 119,871,389 bp
  • T to A, chromosome 6 at 39,643,163 bp
  • A to T, chromosome 6 at 71,885,315 bp
  • T to C, chromosome 6 at 102,278,404 bp
  • A to G, chromosome 6 at 124,317,714 bp
  • G to A, chromosome 6 at 141,765,742 bp
  • A to T, chromosome 7 at 16,248,912 bp
  • T to A, chromosome 7 at 38,519,223 bp
  • A to G, chromosome 7 at 43,456,554 bp
  • A to T, chromosome 7 at 80,325,655 bp
  • G to T, chromosome 7 at 103,320,791 bp
  • A to T, chromosome 7 at 139,242,624 bp
  • C to T, chromosome 8 at 3,258,752 bp
  • A to T, chromosome 8 at 47,847,465 bp
  • C to T, chromosome 8 at 48,236,439 bp
  • T to C, chromosome 8 at 79,248,424 bp
  • T to G, chromosome 9 at 15,919,221 bp
  • T to A, chromosome 9 at 39,950,637 bp
  • A to T, chromosome 9 at 42,416,117 bp
  • C to T, chromosome 9 at 50,753,448 bp
  • A to G, chromosome 10 at 108,240,837 bp
  • TCATCTTCCA to TCA, chromosome 11 at 3,250,294 bp
  • C to T, chromosome 11 at 5,071,573 bp
  • A to G, chromosome 11 at 51,624,600 bp
  • T to A, chromosome 11 at 70,216,016 bp
  • T to A, chromosome 11 at 77,859,074 bp
  • G to T, chromosome 11 at 99,665,719 bp
  • T to C, chromosome 11 at 116,152,784 bp
  • C to T, chromosome 13 at 42,158,714 bp
  • ACTTCTTCT to ACTTCT, chromosome 13 at 59,649,576 bp
  • T to A, chromosome 13 at 95,701,134 bp
  • A to T, chromosome 13 at 102,735,974 bp
  • C to T, chromosome 13 at 102,804,647 bp
  • A to G, chromosome 14 at 55,608,957 bp
  • C to T, chromosome 15 at 101,815,960 bp
  • A to T, chromosome 16 at 36,049,845 bp
  • A to G, chromosome 17 at 15,527,081 bp
  • G to A, chromosome 17 at 22,145,980 bp
  • T to C, chromosome 17 at 22,603,214 bp
  • G to A, chromosome 17 at 25,124,924 bp
  • A to C, chromosome 17 at 34,157,350 bp
  • A to G, chromosome 17 at 34,199,643 bp
  • A to G, chromosome 17 at 36,187,070 bp
  • T to C, chromosome 17 at 53,638,522 bp
  • A to G, chromosome 18 at 37,749,256 bp
  • T to C, chromosome 18 at 46,882,298 bp
  • C to A, chromosome 18 at 60,943,175 bp
  • G to T, chromosome 19 at 4,858,483 bp
  • T to A, chromosome 19 at 5,465,837 bp
  • T to C, chromosome 19 at 29,586,613 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6993 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045099-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.