MMRRC:045285-MU

C to A, chromosome 1 at 104,954,071 bp
T to A, chromosome 1 at 135,850,376 bp
T to C, chromosome 1 at 153,741,670 bp
A to T, chromosome 2 at 32,287,910 bp
A to G, chromosome 2 at 37,085,658 bp
A to G, chromosome 2 at 80,540,198 bp
G to T, chromosome 2 at 84,622,046 bp
A to G, chromosome 2 at 166,963,574 bp
A to T, chromosome 3 at 86,799,602 bp
A to G, chromosome 4 at 20,282,892 bp
A to G, chromosome 4 at 60,659,726 bp
T to G, chromosome 4 at 103,066,352 bp
T to A, chromosome 4 at 113,539,339 bp
T to A, chromosome 4 at 114,231,747 bp
A to G, chromosome 4 at 129,631,278 bp
G to A, chromosome 4 at 133,195,734 bp
A to T, chromosome 4 at 136,999,626 bp
T to A, chromosome 5 at 30,870,721 bp
A to T, chromosome 5 at 45,523,452 bp
T to C, chromosome 5 at 134,411,094 bp
G to A, chromosome 5 at 137,291,489 bp
C to T, chromosome 5 at 149,595,371 bp
T to C, chromosome 6 at 3,687,612 bp
CCACATCAGGATCCACATCAGGATGCACATCAGCATCAGGATCCCCATCAGGATGCACATCAGGATCCACATCAGGATGCACATCAG to CCACATCAGGATCCACATCAGGATGCACATCAG, chromosome 6 at 4,756,398 bp
T to C, chromosome 6 at 23,074,630 bp
G to A, chromosome 6 at 102,278,422 bp
T to G, chromosome 6 at 111,358,569 bp
T to C, chromosome 6 at 124,061,989 bp
C to T, chromosome 6 at 127,689,829 bp
C to T, chromosome 7 at 19,080,309 bp
T to A, chromosome 7 at 28,104,021 bp
A to G, chromosome 7 at 28,467,139 bp
C to T, chromosome 7 at 30,252,336 bp
A to T, chromosome 7 at 45,336,565 bp
T to C, chromosome 7 at 67,734,915 bp
A to C, chromosome 7 at 105,902,179 bp
C to A, chromosome 8 at 24,711,401 bp
C to T, chromosome 8 at 25,919,637 bp
T to C, chromosome 8 at 75,121,716 bp
C to A, chromosome 9 at 7,141,059 bp
A to T, chromosome 9 at 37,424,724 bp
A to T, chromosome 9 at 53,512,008 bp
C to T, chromosome 11 at 103,360,759 bp
T to C, chromosome 11 at 114,757,162 bp
A to G, chromosome 11 at 117,776,325 bp
A to G, chromosome 12 at 55,308,645 bp
A to T, chromosome 12 at 76,031,398 bp
T to A, chromosome 12 at 103,728,294 bp
G to C, chromosome 13 at 13,468,385 bp
T to A, chromosome 13 at 34,884,011 bp
T to C, chromosome 13 at 91,781,242 bp
G to T, chromosome 13 at 119,701,084 bp
T to G, chromosome 14 at 8,241,303 bp
A to T, chromosome 14 at 14,112,225 bp
T to A, chromosome 14 at 50,824,556 bp
C to T, chromosome 14 at 65,931,266 bp
T to A, chromosome 14 at 70,174,377 bp
A to G, chromosome 14 at 72,451,752 bp
G to T, chromosome 15 at 35,073,116 bp
A to C, chromosome 15 at 37,335,736 bp
T to C, chromosome 15 at 66,604,789 bp
T to C, chromosome 15 at 68,180,007 bp
G to T, chromosome 15 at 91,190,682 bp
T to C, chromosome 15 at 100,402,332 bp
T to C, chromosome 16 at 36,756,281 bp
T to A, chromosome 16 at 38,753,940 bp
T to G, chromosome 16 at 45,096,710 bp
A to T, chromosome 16 at 78,575,926 bp
T to G, chromosome 16 at 91,662,102 bp
C to T, chromosome 16 at 96,035,959 bp
A to T, chromosome 17 at 17,552,910 bp
G to A, chromosome 17 at 27,196,191 bp
G to A, chromosome 17 at 31,420,284 bp
A to G, chromosome 17 at 35,067,411 bp
T to C, chromosome 18 at 36,625,028 bp
T to C, chromosome 18 at 46,287,631 bp
C to T, chromosome 19 at 12,482,073 bp
G to C, chromosome X at 106,435,073 bp

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.

Strain GQC Summary

Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice

Littermates of all relevant genotypes.

Strain Origin

Donor

Bruce Beutler, M.D., University of Texas Southwestern Medical Center.

Primary Reference

MUTAGENETIX record for R7208 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Strain Development

C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.

Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information Expand All

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat Color

Black

Breeding

MMRRC Breeding System

Random intra-strain mating

Breeding Scheme(s)

When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.

Overall Breeding Performance

Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain

Reproductive Statistics

Average litter size

Undetermined

Recommended wean age

3 weeks

Order Information Expand All

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)

MMRRC Item #	Description	Distribution Fee / Unit (US $) Shipping & Handling not included	Units	Notes
045285-MU-RESUS	Litter recovered from cryo-archive	$5,475.00 / Non-Profit	Litter	Recovered litter⁴; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

¹ The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

² An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

³ An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

⁴ The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.

Strain Detail Sheet

Strain Information Expand All

Colony and Husbandry Information Expand All

Order Information Expand All