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Strain Name:
C57BL/6J-MtgxR7215Btlr/Mmmh
Stock Number:
045287-MU
Citation ID:
RRID:MMRRC_045287-MU
Other Names:
R7215 (G1)
Major Collection:

Strain Information

Erbb4
Name: erb-b2 receptor tyrosine kinase 4
Synonyms: ErbB4, Her4
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 13869
HGNC: HGNC:3432
Homologene: 21084
Inpp5d
Name: inositol polyphosphate-5-phosphatase D
Synonyms: SHIP, Src homology 2 domain-containing inositol-5-phosphatase, s-SHIP, SHIP-1, SHIP1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 16331
HGNC: HGNC:6079
Homologene: 4046
Btbd17
Name: BTB domain containing 17
Synonyms: 1500005I02Rik, Tango10a
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 72014
Homologene: 46324
Macf1
Name: microtubule-actin crosslinking factor 1
Synonyms: Acf7, Aclp7, trabeculin alpha
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11426
Homologene: 136191
Nsd1
Name: nuclear receptor-binding SET-domain protein 1
Synonyms: KMT3B
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 18193
VEGA: 13
Homologene: 32543
Prrc2b
Name: proline-rich coiled-coil 2B
Synonyms: 5830434P21Rik, Bat2l
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227723
Homologene: 106649
Mbtps1
Name: membrane-bound transcription factor peptidase, site 1
Synonyms: subtilisin/kexin isozyme-1, SKI-1, S1P, 0610038M03Rik, site-1 protease
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 56453
Homologene: 2808
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 4,867,483 bp
  • G to A, chromosome 1 at 53,618,350 bp
  • A to T, chromosome 1 at 68,339,460 bp
  • A to T, chromosome 1 at 87,701,218 bp
  • A to C, chromosome 1 at 155,894,707 bp
  • A to T, chromosome 1 at 156,690,553 bp
  • G to T, chromosome 2 at 22,245,567 bp
  • A to G, chromosome 2 at 25,350,390 bp
  • G to A, chromosome 2 at 32,229,297 bp
  • C to T, chromosome 2 at 86,159,456 bp
  • G to A, chromosome 2 at 89,112,501 bp
  • T to A, chromosome 2 at 110,665,932 bp
  • C to A, chromosome 2 at 152,628,149 bp
  • A to G, chromosome 3 at 54,194,896 bp
  • A to G, chromosome 3 at 96,880,088 bp
  • A to G, chromosome 3 at 119,266,032 bp
  • C to T, chromosome 4 at 88,760,730 bp
  • T to C, chromosome 4 at 108,527,008 bp
  • T to C, chromosome 4 at 123,507,304 bp
  • T to C, chromosome 4 at 151,144,737 bp
  • A to C, chromosome 5 at 24,388,041 bp
  • G to T, chromosome 5 at 30,119,842 bp
  • A to G, chromosome 5 at 81,693,550 bp
  • G to A, chromosome 5 at 92,323,888 bp
  • G to A, chromosome 5 at 144,797,135 bp
  • A to G, chromosome 6 at 86,462,680 bp
  • A to T, chromosome 6 at 123,704,364 bp
  • T to A, chromosome 6 at 128,228,678 bp
  • C to T, chromosome 6 at 139,754,863 bp
  • C to T, chromosome 7 at 3,822,311 bp
  • A to G, chromosome 7 at 30,717,360 bp
  • T to A, chromosome 7 at 39,411,371 bp
  • T to C, chromosome 7 at 41,400,286 bp
  • T to A, chromosome 7 at 44,200,404 bp
  • C to T, chromosome 7 at 45,936,622 bp
  • A to G, chromosome 7 at 102,112,208 bp
  • T to C, chromosome 7 at 120,795,630 bp
  • T to C, chromosome 7 at 121,118,572 bp
  • A to G, chromosome 7 at 127,391,523 bp
  • T to A, chromosome 7 at 127,905,110 bp
  • G to A, chromosome 7 at 133,587,418 bp
  • A to T, chromosome 8 at 84,909,770 bp
  • A to G, chromosome 8 at 119,524,568 bp
  • A to T, chromosome 8 at 122,599,348 bp
  • A to G, chromosome 9 at 5,298,523 bp
  • A to G, chromosome 9 at 38,673,447 bp
  • T to A, chromosome 9 at 64,928,503 bp
  • C to G, chromosome 10 at 24,888,429 bp
  • T to A, chromosome 10 at 61,211,596 bp
  • C to T, chromosome 10 at 80,025,482 bp
  • T to A, chromosome 10 at 116,338,776 bp
  • T to A, chromosome 11 at 9,288,405 bp
  • T to C, chromosome 11 at 60,801,535 bp
  • A to T, chromosome 11 at 76,965,899 bp
  • T to A, chromosome 11 at 87,770,489 bp
  • T to A, chromosome 11 at 101,215,299 bp
  • T to C, chromosome 11 at 106,695,919 bp
  • T to C, chromosome 11 at 114,791,465 bp
  • T to C, chromosome 11 at 118,265,154 bp
  • T to A, chromosome 12 at 85,933,396 bp
  • T to G, chromosome 13 at 3,941,365 bp
  • T to A, chromosome 13 at 55,247,641 bp
  • G to A, chromosome 13 at 77,323,571 bp
  • T to C, chromosome 13 at 88,822,050 bp
  • A to T, chromosome 14 at 31,160,797 bp
  • A to T, chromosome 14 at 50,944,583 bp
  • T to A, chromosome 14 at 52,725,342 bp
  • T to A, chromosome 15 at 44,528,163 bp
  • A to G, chromosome 15 at 57,823,643 bp
  • A to T, chromosome 15 at 71,970,332 bp
  • A to G, chromosome 15 at 77,927,686 bp
  • A to T, chromosome 15 at 82,853,489 bp
  • G to A, chromosome 15 at 101,029,830 bp
  • A to G, chromosome 16 at 17,139,928 bp
  • G to A, chromosome 16 at 97,747,323 bp
  • G to T, chromosome 17 at 29,319,098 bp
  • A to T, chromosome 17 at 34,629,703 bp
  • T to C, chromosome 18 at 35,681,981 bp
  • A to T, chromosome 18 at 37,505,386 bp
  • C to T, chromosome 18 at 52,907,343 bp
  • T to A, chromosome 18 at 78,856,837 bp
  • T to A, chromosome 19 at 6,297,642 bp
  • G to A, chromosome 19 at 40,046,182 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7215 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045287-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.