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Strain Name:
C57BL/6J-MtgxR7211Btlr/Mmmh
Stock Number:
045339-MU
Citation ID:
RRID:MMRRC_045339-MU
Other Names:
R7211 (G1)
Major Collection:

Strain Information

a
Name: nonagouti
Synonyms: agouti, As, agouti signal protein, ASP, Asip
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 50518
HGNC: HGNC:745
Homologene: 1264
Utrn
Name: utrophin
Synonyms: G-utrophin, Dmdl, DRP
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 22288
VEGA: 10
Homologene: 21398
Angpt2
Name: angiopoietin 2
Synonyms: Ang2, Ang-2
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 11601
HGNC: HGNC:485
Homologene: 22401
Gaa
Name: glucosidase, alpha, acid
Synonyms: E430018M07Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 14387
HGNC: HGNC:4065
Homologene: 37268
Atm
Name: ataxia telangiectasia mutated
Synonyms: C030026E19Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 11920
HGNC: HGNC:795
Homologene: 30952
Nudt1
Name: nudix hydrolase 1
Synonyms: 8-oxo-7,8-dihydro-2'-dGTPase, Mth1, nudix (nucleoside diphosphate linked moiety X)-type motif 1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 17766
HGNC: HGNC:8048
Homologene: 1839
Dnajc2
Name: DnaJ heat shock protein family (Hsp40) member C2
Synonyms: MIDA1, Mida1, Zrf1, Zrf2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 22791
Homologene: 31656
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to G, chromosome 1 at 60,200,951 bp
  • C to T, chromosome 2 at 26,989,298 bp
  • A to T, chromosome 2 at 36,122,893 bp
  • A to T, chromosome 2 at 54,085,258 bp
  • T to C, chromosome 2 at 76,767,688 bp
  • C to A, chromosome 2 at 80,418,325 bp
  • A to G, chromosome 2 at 114,134,723 bp
  • G to A, chromosome 2 at 121,304,643 bp
  • A to G, chromosome 2 at 127,247,008 bp
  • A to C, chromosome 2 at 132,249,872 bp
  • T to A, chromosome 2 at 155,045,779 bp
  • G to A, chromosome 2 at 160,731,874 bp
  • A to T, chromosome 3 at 36,640,733 bp
  • T to A, chromosome 3 at 56,004,901 bp
  • G to T, chromosome 3 at 87,743,326 bp
  • A to T, chromosome 3 at 100,959,307 bp
  • A to G, chromosome 3 at 122,957,650 bp
  • A to G, chromosome 3 at 144,814,014 bp
  • A to G, chromosome 3 at 146,913,276 bp
  • A to G, chromosome 4 at 41,498,028 bp
  • T to C, chromosome 4 at 113,238,369 bp
  • C to A, chromosome 4 at 148,241,522 bp
  • C to T, chromosome 5 at 21,776,779 bp
  • T to C, chromosome 5 at 24,995,298 bp
  • T to A, chromosome 5 at 32,830,087 bp
  • T to C, chromosome 5 at 64,105,850 bp
  • T to C, chromosome 5 at 72,782,012 bp
  • T to C, chromosome 5 at 87,001,318 bp
  • T to C, chromosome 5 at 138,178,826 bp
  • C to A, chromosome 5 at 140,337,647 bp
  • A to T, chromosome 6 at 16,867,465 bp
  • A to T, chromosome 6 at 34,421,114 bp
  • A to T, chromosome 6 at 42,818,016 bp
  • T to C, chromosome 6 at 49,052,015 bp
  • A to T, chromosome 6 at 121,880,539 bp
  • A to T, chromosome 6 at 124,778,189 bp
  • C to T, chromosome 7 at 16,140,613 bp
  • C to T, chromosome 7 at 31,375,653 bp
  • T to A, chromosome 7 at 45,448,260 bp
  • T to A, chromosome 8 at 18,741,131 bp
  • T to A, chromosome 8 at 43,625,877 bp
  • T to A, chromosome 8 at 105,886,309 bp
  • T to G, chromosome 9 at 18,498,570 bp
  • A to C, chromosome 9 at 39,819,858 bp
  • G to T, chromosome 9 at 51,290,433 bp
  • A to G, chromosome 9 at 53,488,560 bp
  • A to G, chromosome 9 at 75,682,084 bp
  • T to A, chromosome 9 at 98,574,145 bp
  • A to G, chromosome 9 at 102,509,150 bp
  • T to A, chromosome 9 at 105,928,164 bp
  • A to T, chromosome 9 at 110,150,014 bp
  • T to C, chromosome 10 at 11,343,675 bp
  • T to C, chromosome 10 at 12,401,335 bp
  • T to A, chromosome 10 at 39,005,495 bp
  • C to A, chromosome 10 at 90,511,070 bp
  • A to T, chromosome 10 at 100,447,605 bp
  • G to T, chromosome 10 at 105,573,726 bp
  • C to T, chromosome 11 at 85,834,714 bp
  • A to G, chromosome 11 at 93,885,380 bp
  • C to A, chromosome 11 at 104,710,713 bp
  • G to T, chromosome 11 at 104,724,609 bp
  • T to C, chromosome 11 at 119,284,204 bp
  • T to G, chromosome 12 at 29,984,904 bp
  • G to T, chromosome 12 at 85,175,775 bp
  • T to A, chromosome 12 at 100,061,817 bp
  • T to A, chromosome 12 at 112,811,012 bp
  • T to A, chromosome 13 at 38,188,535 bp
  • A to G, chromosome 14 at 20,077,508 bp
  • T to C, chromosome 14 at 66,829,976 bp
  • A to G, chromosome 14 at 75,274,990 bp
  • T to C, chromosome 15 at 58,053,251 bp
  • A to T, chromosome 15 at 78,773,068 bp
  • A to C, chromosome 15 at 102,955,052 bp
  • T to C, chromosome 16 at 17,698,113 bp
  • A to G, chromosome 16 at 44,788,757 bp
  • T to C, chromosome 17 at 12,586,883 bp
  • T to C, chromosome 17 at 13,052,217 bp
  • A to G, chromosome 17 at 34,735,534 bp
  • T to A, chromosome 18 at 37,709,120 bp
  • A to G, chromosome 18 at 61,989,403 bp
  • G to T, chromosome 19 at 6,907,590 bp
  • A to G, chromosome 19 at 29,786,312 bp
  • A to G, chromosome 19 at 31,927,141 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7211 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045339-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.