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Strain Detail Sheet

Published:
Revised: 03/02/2024

Strain Name:
C57BL/6J-MtgxR7583Btlr/Mmmh
Stock Number:
045666-MU
Citation ID:
RRID:MMRRC_045666-MU
Other Names:
R7583 (G1)
Major Collection:
Beutler Mutagenetix

Strain Information

Gene Details

Col2a1
Name: collagen, type II, alpha 1
Synonyms: Del1, Col2a-1, Col2a, Col2, M100856, Rgsc856, Lpk, M100413, Rgsc413
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 12824
HGNC: HGNC:2200
Homologene: 55607

Additional Resources
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Gene Ontology
BioGPS
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KOMP Phenotyping
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ClinGen
Nefh
Name: neurofilament, heavy polypeptide
Synonyms: NF-H, NF200, NEFH
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 380684
HGNC: HGNC:7737
Homologene: 40755

Additional Resources
IMPC
Google
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Gene Ontology
BioGPS
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Mouse Phenome DB
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ClinGen
Lmf1
Name: lipase maturation factor 1
Synonyms: 2400010G15Rik, Tmem112
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 76483
Homologene: 23384

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ClinGen
Lyst
Name: lysosomal trafficking regulator
Synonyms: D13Sfk13
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 17101
VEGA: 13
HGNC: HGNC:1968
Homologene: 61

Additional Resources
IMPC
Google
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Gene Ontology
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ClinGen
Snx17
Name: sorting nexin 17
Synonyms: 5830447M19Rik, D5Ertd260e, b2b1625.1Clo
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 266781
Homologene: 8838

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Ticrr
Name: TOPBP1-interacting checkpoint and replication regulator
Synonyms: 5730590G19Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 77011
Homologene: 67120

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Luc7l2
Name: LUC7-like 2 (S. cerevisiae)
Synonyms: CGI-74, CGI-59, 4930471C18Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 192196
Homologene: 56737

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 8,445,025 bp
  • A to T, chromosome 1 at 51,289,618 bp
  • T to C, chromosome 1 at 127,930,875 bp
  • C to T, chromosome 1 at 131,276,479 bp
  • T to A, chromosome 1 at 153,243,232 bp
  • T to A, chromosome 1 at 162,477,774 bp
  • A to G, chromosome 1 at 165,333,310 bp
  • G to A, chromosome 1 at 178,530,445 bp
  • A to G, chromosome 2 at 26,973,953 bp
  • T to A, chromosome 2 at 27,518,775 bp
  • A to C, chromosome 2 at 36,997,080 bp
  • A to T, chromosome 2 at 37,105,527 bp
  • A to G, chromosome 2 at 37,790,595 bp
  • A to T, chromosome 2 at 68,739,326 bp
  • A to G, chromosome 2 at 82,975,241 bp
  • A to T, chromosome 2 at 84,612,061 bp
  • A to T, chromosome 2 at 89,590,751 bp
  • C to A, chromosome 2 at 118,862,131 bp
  • T to A, chromosome 2 at 181,237,572 bp
  • T to C, chromosome 3 at 30,691,099 bp
  • T to C, chromosome 3 at 88,912,929 bp
  • T to A, chromosome 3 at 100,925,023 bp
  • A to T, chromosome 3 at 103,902,114 bp
  • G to A, chromosome 4 at 62,192,016 bp
  • A to T, chromosome 4 at 111,118,953 bp
  • T to A, chromosome 4 at 147,583,489 bp
  • A to G, chromosome 5 at 31,196,533 bp
  • T to C, chromosome 5 at 41,833,790 bp
  • A to G, chromosome 5 at 73,434,289 bp
  • A to T, chromosome 5 at 94,682,894 bp
  • A to C, chromosome 5 at 129,179,588 bp
  • T to C, chromosome 5 at 135,852,281 bp
  • T to G, chromosome 5 at 137,607,445 bp
  • A to T, chromosome 6 at 17,942,754 bp
  • A to G, chromosome 6 at 38,551,885 bp
  • A to T, chromosome 6 at 85,118,700 bp
  • T to C, chromosome 6 at 124,779,578 bp
  • A to G, chromosome 6 at 129,659,311 bp
  • A to T, chromosome 7 at 8,194,741 bp
  • T to A, chromosome 7 at 18,682,483 bp
  • C to A, chromosome 7 at 26,759,064 bp
  • T to A, chromosome 7 at 42,788,042 bp
  • C to A, chromosome 7 at 79,696,739 bp
  • C to T, chromosome 7 at 80,366,944 bp
  • T to C, chromosome 7 at 122,127,342 bp
  • G to A, chromosome 7 at 127,137,062 bp
  • G to A, chromosome 7 at 132,983,896 bp
  • A to G, chromosome 7 at 140,574,210 bp
  • G to A, chromosome 8 at 13,785,889 bp
  • A to G, chromosome 8 at 15,998,833 bp
  • A to G, chromosome 8 at 25,971,564 bp
  • A to T, chromosome 8 at 94,555,581 bp
  • A to G, chromosome 8 at 105,363,272 bp
  • A to T, chromosome 8 at 117,454,952 bp
  • G to A, chromosome 8 at 122,570,256 bp
  • A to C, chromosome 9 at 21,835,914 bp
  • G to T, chromosome 9 at 27,071,848 bp
  • G to T, chromosome 9 at 27,400,433 bp
  • A to G, chromosome 9 at 113,908,687 bp
  • A to T, chromosome 10 at 24,836,092 bp
  • A to T, chromosome 10 at 53,281,354 bp
  • T to A, chromosome 10 at 59,369,640 bp
  • G to A, chromosome 10 at 80,709,458 bp
  • T to C, chromosome 11 at 4,941,089 bp
  • A to G, chromosome 11 at 55,213,928 bp
  • C to T, chromosome 11 at 67,220,913 bp
  • A to G, chromosome 11 at 73,188,470 bp
  • A to T, chromosome 11 at 73,711,107 bp
  • T to G, chromosome 12 at 84,308,405 bp
  • C to T, chromosome 12 at 105,811,045 bp
  • A to T, chromosome 13 at 13,635,887 bp
  • C to A, chromosome 13 at 24,975,760 bp
  • T to A, chromosome 13 at 42,164,240 bp
  • T to A, chromosome 13 at 100,049,812 bp
  • T to C, chromosome 13 at 100,779,754 bp
  • T to C, chromosome 14 at 52,349,903 bp
  • T to A, chromosome 14 at 62,606,559 bp
  • T to C, chromosome 15 at 44,568,364 bp
  • A to T, chromosome 15 at 55,064,676 bp
  • T to C, chromosome 15 at 58,126,664 bp
  • T to G, chromosome 15 at 66,764,418 bp
  • G to T, chromosome 15 at 75,048,497 bp
  • A to T, chromosome 15 at 81,237,441 bp
  • T to C, chromosome 15 at 82,855,276 bp
  • T to G, chromosome 15 at 97,976,184 bp
  • T to C, chromosome 16 at 14,404,038 bp
  • A to G, chromosome 16 at 44,833,421 bp
  • G to A, chromosome 17 at 25,655,449 bp
  • A to T, chromosome 17 at 25,825,830 bp
  • G to T, chromosome 17 at 43,624,238 bp
  • A to T, chromosome 17 at 67,761,621 bp
  • G to T, chromosome 17 at 71,231,396 bp
  • A to G, chromosome 18 at 37,682,324 bp
  • T to A, chromosome 19 at 3,490,790 bp
  • T to A, chromosome 19 at 9,006,093 bp
  • A to T, chromosome 19 at 55,235,615 bp
  • T to A, chromosome 19 at 56,311,804 bp
  • T to A, chromosome 19 at 57,378,602 bp
  • G to T, chromosome 19 at 61,083,204 bp

Strain Description

Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.

Strain Origin

Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7583 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat Color
Black

Breeding

MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain

Reproductive Statistics

Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
045666-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.