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Strain Name:
C57BL/6J-MtgxR7599Btlr/Mmmh
Stock Number:
045673-MU
Citation ID:
RRID:MMRRC_045673-MU
Other Names:
R7599 (G1)
Major Collection:

Strain Information

Ptprz1
Name: protein tyrosine phosphatase receptor type Z, polypeptide 1
Synonyms: DSD-1-PG, phosphacan, PTPzeta, PTPbeta, Rptpbeta, Ptpz, Ptprz, RPTPz
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 19283
HGNC: HGNC:9685
Homologene: 2136
Pmp22
Name: peripheral myelin protein 22
Synonyms: Gas-3, TRE002
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 18858
HGNC: HGNC:9118
Homologene: 7482
Ptpn14
Name: protein tyrosine phosphatase, non-receptor type 14
Synonyms: PTP36, C130080N23Rik, OTTMUSG00000022087
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 19250
HGNC: HGNC:9647
Homologene: 3941
Hdac1
Name: histone deacetylase 1
Synonyms: RPD3, HD1, MommeD5
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 433759
HGNC: HGNC:4852
Homologene: 68426
Wnk1
Name: WNK lysine deficient protein kinase 1
Synonyms: 6430573H23Rik, Prkwnk1, EG406236, Hsn2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 232341
Homologene: 14253
Snapc3
Name: small nuclear RNA activating complex, polypeptide 3
Synonyms: 5031401C21Rik, 4930558A07Rik, 1810020H02Rik, E030018J20Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 77634
Homologene: 31130
Kpna2
Name: karyopherin subunit alpha 2
Synonyms: pendulin, m-importin, m-importin-alpha-P1, Rch1, 2410044B12Rik, Importin alpha, importin alpha 1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 16647
HGNC: HGNC:6395
Homologene: 1708
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 189,850,745 bp
  • G to T, chromosome 2 at 31,356,286 bp
  • T to A, chromosome 2 at 35,050,419 bp
  • TGGGG to TGGG, chromosome 2 at 37,502,896 bp
  • C to T, chromosome 2 at 40,661,549 bp
  • T to G, chromosome 2 at 44,994,613 bp
  • T to A, chromosome 2 at 89,609,227 bp
  • T to C, chromosome 2 at 154,214,151 bp
  • A to C, chromosome 2 at 162,964,514 bp
  • T to C, chromosome 2 at 180,471,255 bp
  • A to G, chromosome 3 at 28,698,017 bp
  • T to C, chromosome 3 at 29,956,385 bp
  • A to G, chromosome 3 at 55,140,109 bp
  • G to T, chromosome 3 at 89,099,386 bp
  • TGC to TGCAGC, chromosome 3 at 130,040,811 bp
  • G to A, chromosome 4 at 4,778,207 bp
  • C to A, chromosome 4 at 83,417,836 bp
  • T to C, chromosome 4 at 86,811,612 bp
  • T to C, chromosome 4 at 111,877,740 bp
  • G to T, chromosome 4 at 129,517,466 bp
  • T to C, chromosome 5 at 89,861,397 bp
  • C to T, chromosome 6 at 23,002,519 bp
  • A to G, chromosome 6 at 70,193,587 bp
  • C to A, chromosome 6 at 119,929,828 bp
  • A to G, chromosome 6 at 125,374,660 bp
  • T to A, chromosome 6 at 135,226,876 bp
  • T to C, chromosome 7 at 24,586,668 bp
  • T to A, chromosome 7 at 27,131,359 bp
  • A to G, chromosome 7 at 43,784,427 bp
  • A to T, chromosome 7 at 79,810,969 bp
  • ATGGCG to ATGGCGACGGTGGCG, chromosome 7 at 97,579,904 bp
  • G to A, chromosome 7 at 128,148,090 bp
  • T to A, chromosome 8 at 25,201,285 bp
  • T to C, chromosome 8 at 45,278,113 bp
  • A to G, chromosome 8 at 123,271,260 bp
  • A to T, chromosome 9 at 20,437,833 bp
  • G to A, chromosome 9 at 35,205,636 bp
  • T to C, chromosome 9 at 108,986,224 bp
  • T to C, chromosome 10 at 4,413,404 bp
  • A to G, chromosome 10 at 26,263,813 bp
  • A to T, chromosome 10 at 56,151,833 bp
  • T to C, chromosome 10 at 80,038,570 bp
  • T to A, chromosome 10 at 81,272,346 bp
  • T to A, chromosome 10 at 128,277,197 bp
  • C to T, chromosome 11 at 41,967,624 bp
  • C to A, chromosome 11 at 63,158,348 bp
  • T to A, chromosome 11 at 73,121,960 bp
  • A to G, chromosome 11 at 97,500,343 bp
  • T to C, chromosome 11 at 106,998,757 bp
  • G to C, chromosome 12 at 9,024,886 bp
  • G to C, chromosome 12 at 75,966,371 bp
  • A to G, chromosome 12 at 84,331,856 bp
  • A to T, chromosome 12 at 85,268,199 bp
  • T to C, chromosome 12 at 89,512,062 bp
  • C to A, chromosome 13 at 40,860,867 bp
  • T to A, chromosome 13 at 58,381,835 bp
  • A to T, chromosome 13 at 66,917,148 bp
  • A to T, chromosome 14 at 51,895,031 bp
  • A to G, chromosome 15 at 10,533,680 bp
  • T to A, chromosome 15 at 25,971,641 bp
  • T to A, chromosome 15 at 58,627,589 bp
  • T to C, chromosome 15 at 83,547,671 bp
  • T to A, chromosome 15 at 83,613,452 bp
  • T to A, chromosome 15 at 83,870,988 bp
  • A to G, chromosome 15 at 99,603,771 bp
  • C to A, chromosome 16 at 36,875,396 bp
  • C to T, chromosome 16 at 58,473,437 bp
  • T to A, chromosome 17 at 28,297,117 bp
  • T to A, chromosome 17 at 49,480,727 bp
  • C to T, chromosome 18 at 80,972,052 bp
  • G to T, chromosome 19 at 6,913,846 bp
  • C to A, chromosome 19 at 12,639,808 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7599 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045673-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.