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Strain Name:
C57BL/6J-MtgxR7831Btlr/Mmmh
Stock Number:
045885-MU
Citation ID:
RRID:MMRRC_045885-MU
Other Names:
R7831 (G1)
Major Collection:

Strain Information

Pald1
Name: phosphatase domain containing, paladin 1
Synonyms: paladin, X99384
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 27355
VEGA: 10
Homologene: 8453
Dicer1
Name: dicer 1, ribonuclease type III
Synonyms: 1110006F08Rik, D12Ertd7e, Dicer1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 192119
VEGA: 12
Homologene: 13251
Tnrc6b
Name: trinucleotide repeat containing 6b
Synonyms: A730065C02Rik, D230019K20Rik, 2700090M07Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 213988
VEGA: 15
Homologene: 66194
Card11
Name: caspase recruitment domain family, member 11
Synonyms: CARMA1, BIMP3, 2410011D02Rik, 0610008L17Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 108723
Homologene: 13024
Fgf8
Name: fibroblast growth factor 8
Synonyms: Fgf-8, Aigf
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 14179
HGNC: HGNC:3686
Homologene: 7715
Pik3cb
Name: phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta
Synonyms: 1110001J02Rik, p110beta
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 74769
HGNC: HGNC:8976
Homologene: 21250
Becn1
Name: beclin 1, autophagy related
Synonyms: Atg6
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 56208
HGNC: HGNC:1034
Homologene: 2794
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 18,128,789 bp
  • G to A, chromosome 1 at 24,526,482 bp
  • A to T, chromosome 1 at 90,796,546 bp
  • G to T, chromosome 1 at 93,215,473 bp
  • G to T, chromosome 1 at 130,719,249 bp
  • G to A, chromosome 1 at 133,071,242 bp
  • G to A, chromosome 1 at 164,145,015 bp
  • G to T, chromosome 1 at 166,389,873 bp
  • A to T, chromosome 1 at 167,840,952 bp
  • T to A, chromosome 1 at 188,759,841 bp
  • A to G, chromosome 2 at 22,866,921 bp
  • T to C, chromosome 2 at 29,157,108 bp
  • T to A, chromosome 2 at 29,179,854 bp
  • C to T, chromosome 2 at 37,219,711 bp
  • C to T, chromosome 2 at 76,881,081 bp
  • C to T, chromosome 2 at 112,926,838 bp
  • T to A, chromosome 2 at 170,485,940 bp
  • G to A, chromosome 3 at 89,184,213 bp
  • T to C, chromosome 4 at 41,614,695 bp
  • T to C, chromosome 4 at 44,995,244 bp
  • A to G, chromosome 4 at 100,441,098 bp
  • G to A, chromosome 4 at 115,250,170 bp
  • C to T, chromosome 5 at 37,319,083 bp
  • A to G, chromosome 5 at 48,244,683 bp
  • A to T, chromosome 5 at 123,613,279 bp
  • A to C, chromosome 5 at 129,901,957 bp
  • G to T, chromosome 5 at 140,873,412 bp
  • CAAAGTAA to CAAAGTAAAGTAA, chromosome 6 at 113,399,157 bp
  • T to A, chromosome 6 at 124,039,799 bp
  • T to G, chromosome 6 at 124,855,155 bp
  • T to A, chromosome 6 at 146,291,584 bp
  • C to T, chromosome 7 at 26,767,140 bp
  • C to T, chromosome 7 at 26,835,515 bp
  • C to A, chromosome 7 at 28,106,979 bp
  • C to A, chromosome 7 at 44,901,012 bp
  • T to A, chromosome 7 at 48,168,249 bp
  • T to A, chromosome 7 at 49,982,800 bp
  • T to A, chromosome 7 at 106,741,413 bp
  • C to T, chromosome 7 at 111,556,458 bp
  • A to G, chromosome 8 at 46,519,006 bp
  • A to T, chromosome 8 at 109,631,358 bp
  • T to C, chromosome 8 at 121,605,245 bp
  • A to G, chromosome 8 at 124,332,078 bp
  • C to A, chromosome 9 at 42,089,961 bp
  • T to G, chromosome 9 at 57,914,635 bp
  • T to C, chromosome 9 at 99,088,613 bp
  • T to C, chromosome 9 at 109,091,990 bp
  • G to A, chromosome 9 at 120,543,959 bp
  • A to G, chromosome 10 at 28,568,408 bp
  • G to T, chromosome 10 at 61,355,814 bp
  • A to G, chromosome 10 at 77,634,742 bp
  • T to A, chromosome 10 at 88,762,770 bp
  • T to G, chromosome 10 at 120,018,106 bp
  • G to A, chromosome 11 at 9,297,404 bp
  • G to A, chromosome 11 at 46,196,446 bp
  • A to T, chromosome 11 at 67,935,956 bp
  • A to C, chromosome 11 at 73,984,431 bp
  • T to C, chromosome 11 at 98,249,827 bp
  • T to C, chromosome 11 at 99,541,261 bp
  • T to C, chromosome 11 at 101,290,453 bp
  • C to T, chromosome 11 at 103,198,173 bp
  • T to A, chromosome 12 at 16,997,631 bp
  • C to T, chromosome 12 at 25,061,231 bp
  • T to A, chromosome 12 at 55,600,538 bp
  • G to A, chromosome 12 at 103,590,821 bp
  • T to G, chromosome 12 at 104,708,800 bp
  • A to T, chromosome 12 at 112,744,800 bp
  • T to G, chromosome 13 at 47,050,622 bp
  • A to C, chromosome 13 at 67,052,419 bp
  • A to T, chromosome 13 at 92,522,328 bp
  • T to A, chromosome 13 at 95,976,692 bp
  • G to A, chromosome 13 at 119,370,094 bp
  • T to A, chromosome 14 at 55,887,846 bp
  • A to T, chromosome 14 at 59,773,753 bp
  • T to G, chromosome 14 at 70,203,032 bp
  • C to G, chromosome 15 at 80,880,379 bp
  • T to A, chromosome 15 at 82,182,087 bp
  • A to G, chromosome 16 at 29,648,937 bp
  • T to A, chromosome 17 at 35,618,759 bp
  • A to C, chromosome 17 at 42,580,619 bp
  • G to A, chromosome 17 at 46,648,672 bp
  • T to A, chromosome 18 at 42,217,562 bp
  • T to A, chromosome 18 at 42,701,532 bp
  • T to C, chromosome 19 at 5,685,961 bp
  • T to C, chromosome 19 at 29,617,967 bp
  • G to A, chromosome 19 at 45,742,437 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7831 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045885-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.