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Availability & Fees Order this Strain
Depending on the type of Cre employed, this strain will lack endogenous expression of Rbpj in the specific tissue where Cre is expressed. Instead, it will allow for the doxycycline-dependent expression of an Rbpj transgene. Thus, this mouse strain will allow for the tissue- and temporal-specific regulation of Notch signaling responsiveness in cells.
Conditional phenotype: Conditional deletion of endogenous Rbpj and inducible expression of transgenic Rbpj.
Chen ELY, Thompson PK, Zúñiga-Pflücker JC. RBPJ-dependent Notch signalinginitiates the T cell program in a subset of thymus-seeding progenitors. NatImmunol. 2019 Nov;20(11):1456-1468. doi: 10.1038/s41590-019-0518-7. Epub 2019 Oct21. (Medline PMID: 31636466)
Han H, Tanigaki K, Yamamoto N, Kuroda K, Yoshimoto M, Nakahata T, Ikuta K, Honjo T. Inducible gene knockout of transcription factor recombination signal binding protein-J reveals its essential role in T versus B lineage decision. Int Immunol. 2002 Jun;14(6):637-45. (Medline PMID: 12039915)
Stadtfeld M, Graf T. Assessing the role of hematopoietic plasticity for endothelial and hepatocyte development by non-invasive lineage tracing. Development. 2005 Jan;132(1):203-13. Epub 2004 Dec 2. (Medline PMID: 15576407)
Belteki G, Haigh J, Kabacs N, Haigh K, Sison K, Costantini F, Whitsett J,Quaggin SE, Nagy A. Conditional and inducible transgene expression in micethrough the combinatorial use of Cre-mediated recombination and tetracyclineinduction. Nucleic Acids Res. 2005 Mar 22;33(5):e51. Erratum in: Nucleic AcidsRes. 2005;33(8):2765. (Medline PMID: 15784609)
Colony Surveillance Program and Current Health Reports
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
The donor or their institution limits the distribution to non-profit institutions only.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.