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Strain Detail Sheet

Strain Name:
B6.Cg-Rbpjtm1Hon Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy Tg(tetO-Rbpj*)1Jczp/Mmmh
Stock Number:
066431-MU
Citation ID:
RRID:MMRRC_066431-MU
Other Names:
RBPJ-inducible

Strain Information

Gene Details

Rbpjtm1.1Hon
Name: recombination signal binding protein for immunoglobulin kappa J region; targeted mutation 1.1, Tasuku Honjo
Synonyms: Rpbjdelta
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Conditional
Rbpj
Name: recombination signal binding protein for immunoglobulin kappa J region
Synonyms: CBF1, Igkrsbp, RBP-J kappa, RBPjk, Igkjrb, Rbpsuh
Type: Gene
Species: Mouse
Chromosome: 5
Alteration at locus: Knock-In
NCBI: 19664
HGNC: HGNC:5724
Homologene: 7511

Additional Resources
IMPC
Google
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PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy
Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Andras Nagy
Synonyms: rtTAflox, ROSA26-rtTA, Gt(ROSA)26Sortm1(rtTA)Nagy, Gt(ROSA)26Sortm1Nagy, Rosa26fs-rtTA, Rosa-eGFP, Rosa-rtTALSL, R26rtTA, ROSA26-rtTA-IRES-EGFP
Type: Allele
Species: Multi-species
Chromosome: 6
Alteration at locus: Knock-In
EGFP
Name: enhanced green fluorescent protein
Type: Gene
Species: Aequorea victoria (jellyfish)
Chromosome:
Alteration at locus: Knock-In
Gt(ROSA)26Sor
Name: gene trap ROSA 26, Philippe Soriano
Synonyms: ROSA26, beta geo, Gtrgeo26, Gtrosa26, R26, Thumpd3as1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Knock-In
NCBI: 14910

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
rtTA
Name: reverse tetracycline transactivator
Type: Gene
Species: Multi-species
Chromosome:
Alteration at locus: Knock-In
Tg(tetO-Rbpj*)1Jczp
Name: transgene insertion 1, Juan-Carlos Zúñiga-Pflücker
Type: Allele
Species: Mus musculus (mouse)
Chromosome: unknown
Alteration at locus: Knock-In
HA
Name: haemagglutinin, Influenza A virus (A/Puerto Rico/8/1934(H1N1))
Type: Gene
Species: Influenza virus
Chromosome:
Alteration at locus: Knock-In
tetO
Name: tetracycline-responsive promoter element
Type: Regulatory Region
Species: Multi-species
Chromosome:
Alteration at locus: Knock-In
Genetic Alterations
Three allelic alterations:

  1. Rbpjtm1Hon: LoxP sites inserted to flank exon 6 and exon 7 of the Rbpj gene.
  2. Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy: A targeting vector containing a loxP-flanked stop sequence, reverse tetracycline-controlled transactivator gene, internal ribosomal entry site, and green fluorescent protein gene inserted between exon 1 and exon 2 of the ROSA26 locus.
  3. Tg(tetO-Rbpj*)1Jczp: Randomly inserted hemagglutinin-tagged Rbpj transgene under the control of a tetracycline-responsive element.
ES Cell Line
  • Gt(ROSA)26Sor allele: R1 derived from (129X1/SvJ x 129S1/Sv)F1-Kitl+
  • Rbpj allele: E14 derived from 129P2/OlaHsd

Strain Description

Phenotype

Depending on the type of Cre employed, this strain will lack endogenous expression of Rbpj in the specific tissue where Cre is expressed. Instead, it will allow for the doxycycline-dependent expression of an Rbpj transgene. Thus, this mouse strain will allow for the tissue- and temporal-specific regulation of Notch signaling responsiveness in cells.

Conditional phenotype: Conditional deletion of endogenous Rbpj and inducible expression of transgenic Rbpj.

MeSH Terms
  • Animals
  • B-Lymphocytes/cytology
  • B-Lymphocytes/immunology
  • B-Lymphocytes/metabolism
  • Bone Marrow Transplantation
  • Cell Differentiation
  • Colony-Forming Units Assay
  • DNA-Binding Proteins/deficiency
  • DNA-Binding Proteins/genetics
  • DNA-Binding Proteins/metabolism
  • Hematopoietic Stem Cells/cytology
  • Hematopoietic Stem Cells/immunology
  • Hematopoietic Stem Cells/metabolism
  • Immunoglobulin J Recombination Signal Sequence-Binding Protein
  • In Vitro Techniques
  • Lymphopoiesis
  • Membrane Proteins/metabolism
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Mice, Transgenic
  • Nuclear Proteins
  • Receptors, Notch
  • Signal Transduction
  • T-Lymphocytes/cytology
  • T-Lymphocytes/immunology
  • T-Lymphocytes/metabolism
  • Bacterial Proteins/metabolism
  • Bone Marrow Cells
  • Cell Lineage
  • Cell Separation
  • Endothelium, Vascular/cytology
  • Endothelium, Vascular/metabolism
  • Flow Cytometry
  • Hepatocytes/cytology
  • Hepatocytes/metabolism
  • Lac Operon
  • Liver/cytology
  • Liver/embryology
  • Liver/pathology
  • Luminescent Proteins/metabolism
  • Macrophages/metabolism
  • Microscopy, Fluorescence
  • Models, Biological
  • Muramidase/metabolism
  • Plasmids/metabolism
  • Polymerase Chain Reaction
  • Regeneration
  • Time Factors
  • Cell Line
  • Doxycycline/pharmacology
  • Embryo, Mammalian/cytology
  • Gene Expression Regulation
  • Gene Targeting
  • Integrases/genetics
  • Integrases/metabolism
  • Mice, Transgenic/genetics
  • Mice, Transgenic/metabolism
  • Proteins/genetics
  • RNA, Untranslated
  • Recombination, Genetic
  • Stem Cells/metabolism
  • Trans-Activators/genetics
  • Trans-Activators/metabolism
  • Transcriptional Activation
  • Transgenes
  • Vascular Endothelial Growth Factor A/biosynthesis
  • Vascular Endothelial Growth Factor A/genetics
  • Viral Proteins/genetics
  • Viral Proteins/metabolism
Strain Development
Rbpj-HA transgenic mice were generated by microinjection of the transgene construct DNA into fertilized eggs obtained from the mating of superovulated C57BL/6J females with C57BL/6J males (JAX Strain #000664) at the University of Michigan Transgenic facility in Ann Arbor. Founders and the F1 progeny were screened by PCR for copy number of the transgene by comparing it to the transgene copy standards. Rbpj-HA mice were then bred to B6.Cg-Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J (JAX Strain #005670), and then further bred to B6.129P2-Rbpjtm1Hon/HonRbrc (RIKEN RBRC01071).

The mice were also bred to B6.Cg-Commd10Tg(Vav1-icre)A2Kio/J (JAX Strain #008610), but this produced Cre expression in hematopoietic cells only, leaving the allele floxed in the material provided to the MMRRC.
Suggested Control Mice
RBPJfloxed/wt; Cre +; rtTA +; RBPJ-HA +

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.

Research Applications

  • Cancer
  • Cell Biology
  • Developmental Biology
  • Immunology and Inflammation

Strain Origin

Donor
Juan-Carlos Zúñiga-Pflücker, Ph.D., Sunnybrook Research Institute.
Andras Nagy, Ph.D., Mount Sinai Hospital.
Tasuku Honjo, M.D., Kyoto University.
Primary Reference

Chen ELY, Thompson PK, Zúñiga-Pflücker JC. RBPJ-dependent Notch signalinginitiates the T cell program in a subset of thymus-seeding progenitors. NatImmunol. 2019 Nov;20(11):1456-1468. doi: 10.1038/s41590-019-0518-7. Epub 2019 Oct21. (Medline PMID: 31636466)

Han H, Tanigaki K, Yamamoto N, Kuroda K, Yoshimoto M, Nakahata T, Ikuta K, Honjo T. Inducible gene knockout of transcription factor recombination signal binding protein-J reveals its essential role in T versus B lineage decision. Int Immunol. 2002 Jun;14(6):637-45. (Medline PMID: 12039915)

Stadtfeld M, Graf T. Assessing the role of hematopoietic plasticity for endothelial and hepatocyte development by non-invasive lineage tracing. Development. 2005 Jan;132(1):203-13. Epub 2004 Dec 2. (Medline PMID: 15576407)

Belteki G, Haigh J, Kabacs N, Haigh K, Sison K, Costantini F, Whitsett J,Quaggin SE, Nagy A. Conditional and inducible transgene expression in micethrough the combinatorial use of Cre-mediated recombination and tetracyclineinduction. Nucleic Acids Res. 2005 Mar 22;33(5):e51. Erratum in: Nucleic AcidsRes. 2005;33(8):2765. (Medline PMID: 15784609)

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

For more information about this colony's health status contact mmrrc@missouri.edu

Appearance

Coat Color
Black
Eye
Black

Breeding

MMRRC Breeding System
Random intra-strain mating
Generation
N/A
Overall Breeding Performance
Good

Reproductive Statistics

NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Hetero/Hemizygotes are fertile: Yes Yes
Age Reproductive Decline: 10 to 12 months 10 to 12 months
Average litter size
7 to 9
Recommended wean age
3 Weeks
Average Pups Weaned
7 to 9

Order Request Information

Availability Level

The availability level for this product has not been determined.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

- Products for this strain are Not Yet Available for Ordering
- If you register interest in this strain, you will be notified when it becomes available for ordering.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.