To map the Tg insertion site and facilitate genotyping, the MMRRC generated a whole-genome sequence of the MMRRC founder transgenic male at 50x coverage using Novaseq of a genomic library of 150bp paired-end reads. Using these data, the MMRRC:
Transgenic mice with airway-targeted overexpression of human ACE2 were generated by microinjection of fertilized (C3H x C57BL/6)F1 (C3B6F1) hybrid oocytes with an expression cassette consisting of the human HFH4 (FOXJ1) lung ciliated epithelial cell-specific promoter and the coding region of ACE2 cDNA in a pTG1 vector. Founder line #1 was crossed to C3B6F1 mice producing human ACE2-transgenic mice, and subsequent progeny were backcrossed for 25+ generations to C57BL/6J.
Menachery VD, Yount BL Jr, Sims AC, Debbink K, Agnihothram SS, Gralinski LE,Graham RL, Scobey T, Plante JA, Royal SR, Swanstrom J, Sheahan TP, Pickles RJ,Corti D, Randell SH, Lanzavecchia A, Marasco WA, Baric RS. SARS-like WIV1-CoVpoised for human emergence. Proc Natl Acad Sci U S A. 2016 Mar15;113(11):3048-53. doi: 10.1073/pnas.1517719113. Epub 2016 Mar 14. (Medline PMID: 26976607)
Jiang RD, Liu MQ, Chen Y, Shan C, Zhou YW, Shen XR, Li Q, Zhang L, Zhu Y, Si HR, Wang Q, Min J, Wang X, Zhang W, Li B, Zhang HJ, Baric RS, Zhou P, Yang XL, Shi ZL. Pathogenesis of SARS-CoV-2 in Transgenic Mice Expressing Human Angiotensin-Converting Enzyme 2. Cell. 2020 Jul 9;182(1):50-58.e8. doi: 10.1016/j.cell.2020.05.027. Epub 2020 May 21. (Medline PMID: 32516571)
|Viability and Fertility:||Female||Male||Comments|
|Homozygotes are viable:||Undetermined||Undetermined|
|Homozygotes are fertile:||Yes||Yes|
|Hetero/Hemizygotes are fertile:||Yes||Yes|
|Age Reproductive Decline:||7 to 9 months||10 to 12 months|
A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Jacqueline Quay
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
|MMRRC Item #||Description||Distribution Fee / Unit (US $)||Units||Notes|
|066719-UNC-RESUS||Litter recovered from cryo-archive||$2,829.00 / $4,696.00
Non-Profit / For-Profit
|Litter||Recovered litter4; additional fees for any special requests.|
|Cryopreserved material may be available upon request, please inquire to email@example.com for more information.|
1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.