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Strain Name:
C57BL/6J-MtgxR8133Btlr/Mmmh
Stock Number:
067561-MU
Citation ID:
RRID:MMRRC_067561-MU
Other Names:
R8133 (G1)
Major Collection:

Strain Information

Cib4
Name: calcium and integrin binding family member 4
Synonyms: 1700041E20Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 73259
Homologene: 18990
Etl4
Name: enhancer trap locus 4
Synonyms: Etl-4, E330027G05Rik, 6620402G01Rik, 9430077C05Rik, Skt, Sickle tail
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 208618
Homologene: 10477
Tanc2
Name: tetratricopeptide repeat, ankyrin repeat and coiled-coil containing 2
Synonyms: 5730590C14Rik, 3526402J09Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 77097
Homologene: 64680
Cct7
Name: chaperonin containing TCP1 subunit 7
Synonyms: Cctz, Ccth
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 12468
HGNC: HGNC:1622
Homologene: 4694
Ryr2
Name: ryanodine receptor 2, cardiac
Synonyms: 9330127I20Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 20191
VEGA: 13
Homologene: 37423
Camsap1
Name: calmodulin regulated spectrin-associated protein 1
Synonyms: PRO2405, 9530003A05Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227634
Homologene: 19202
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • TGCGCGCGCGC to TGCGCGCGC, chromosome 1 at 106,172,792 bp
  • G to T, chromosome 2 at 13,388,848 bp
  • A to G, chromosome 2 at 20,806,271 bp
  • T to A, chromosome 2 at 24,396,689 bp
  • T to C, chromosome 2 at 25,934,297 bp
  • T to C, chromosome 2 at 132,878,014 bp
  • A to G, chromosome 3 at 5,400,494 bp
  • C to T, chromosome 3 at 89,347,206 bp
  • T to C, chromosome 3 at 90,591,309 bp
  • T to C, chromosome 3 at 103,753,394 bp
  • C to T, chromosome 4 at 155,657,001 bp
  • T to C, chromosome 5 at 30,534,218 bp
  • C to A, chromosome 5 at 43,901,448 bp
  • T to C, chromosome 5 at 120,787,995 bp
  • T to C, chromosome 5 at 128,749,850 bp
  • T to C, chromosome 6 at 28,442,514 bp
  • T to C, chromosome 6 at 85,461,063 bp
  • T to C, chromosome 6 at 94,611,629 bp
  • T to A, chromosome 6 at 104,728,337 bp
  • A to G, chromosome 7 at 41,375,065 bp
  • A to G, chromosome 7 at 42,475,057 bp
  • T to A, chromosome 7 at 102,542,858 bp
  • G to T, chromosome 7 at 104,039,915 bp
  • T to C, chromosome 7 at 137,313,143 bp
  • A to G, chromosome 8 at 13,011,487 bp
  • A to T, chromosome 9 at 21,191,377 bp
  • C to A, chromosome 9 at 65,131,741 bp
  • C to A, chromosome 10 at 18,611,203 bp
  • T to A, chromosome 11 at 105,923,222 bp
  • A to T, chromosome 11 at 110,127,463 bp
  • A to T, chromosome 13 at 11,603,698 bp
  • T to A, chromosome 13 at 20,373,086 bp
  • T to C, chromosome 13 at 33,985,478 bp
  • T to C, chromosome 13 at 96,866,495 bp
  • C to T, chromosome 14 at 59,284,324 bp
  • G to T, chromosome 14 at 104,479,245 bp
  • T to C, chromosome 15 at 98,864,942 bp
  • T to C, chromosome 15 at 99,304,438 bp
  • T to C, chromosome 16 at 45,443,941 bp
  • C to T, chromosome 17 at 24,164,579 bp
  • C to T, chromosome 18 at 4,649,384 bp
  • T to A, chromosome 18 at 36,032,377 bp
  • T to A, chromosome 18 at 37,478,132 bp
  • G to T, chromosome 18 at 37,506,610 bp
  • T to G, chromosome 19 at 12,680,480 bp
  • A to G, chromosome 19 at 28,350,006 bp
  • T to C, chromosome 19 at 41,915,710 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8133 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067561-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.