Strain Name:
C57BL/6J-MtgxR8520Btlr/Mmmh
Stock Number:
067947-MU
Citation ID:
RRID:MMRRC_067947-MU
Other Names:
R8520 (G1)
Major Collection:

Strain Information

Utrn
Name: utrophin
Synonyms: G-utrophin, DRP, Dmdl
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 22288
VEGA: 10
Homologene: 21398
Thbs4
Name: thrombospondin 4
Synonyms: TSP-4, TSP4
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 21828
Homologene: 20691
U2surp
Name: U2 snRNP-associated SURP domain containing
Synonyms: 2610101N10Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 67958
Homologene: 13964
Wdr7
Name: WD repeat domain 7
Synonyms: TRAG, TGF-beta resistance associated gene
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 104082
VEGA: 18
Homologene: 11408
Ttk
Name: Ttk protein kinase
Synonyms: Esk1, Mps1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 22137
Homologene: 2489
Topbp1
Name: topoisomerase (DNA) II binding protein 1
Synonyms: D430026L04Rik, 1110031N14Rik, 2810429C13Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235559
Homologene: 38262
Mier2
Name: MIER family member 2
Synonyms: 2700087H15Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 70427
Homologene: 18941
Mcf2l
Name: mcf.2 transforming sequence-like
Synonyms: C130040G20Rik, Dbs
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 17207
Homologene: 11804
Ebf3
Name: early B cell factor 3
Synonyms: 3110018A08Rik, Olf-1/EBF-like 2, O/E-2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 13593
Homologene: 56472
Dsg2
Name: desmoglein 2
Synonyms: D18Ertd293e
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 13511
HGNC: HGNC:3049
Homologene: 1464
Ints7
Name: integrator complex subunit 7
Synonyms: 5930412E23Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 77065
Homologene: 9136
Zfp984
Name: zinc finger protein 984
Synonyms: Gm13157
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 100041677
Homologene: 133076
Cubn
Name: cubilin
Synonyms: D2Wsu88e, intrinsic factor-cobalamin receptor
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 65969
HGNC: HGNC:2548
Homologene: 37434
Hmcn2
Name: hemicentin 2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 665700
Homologene: 90772
Slc4a3
Name: solute carrier family 4 (anion exchanger), member 3
Synonyms: A930038D23Rik, Ae3
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 20536
Homologene: 129474
Dnah14
Name: dynein, axonemal, heavy chain 14
Synonyms: LOC381311, Gm980, A230079K17Rik, Dnahc14
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 240960
HGNC: HGNC:2945
Homologene: 90078
Ankrd52
Name: ankyrin repeat domain 52
Synonyms: G431002C21Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 237615
Homologene: 18227
Il1r2
Name: interleukin 1 receptor, type II
Synonyms: IL-1 receptor beta chain, CD121b, Il1r-2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 16178
HGNC: HGNC:5994
Homologene: 7783
Fbn2
Name: fibrillin 2
Synonyms: Fib-2, sy, Sne
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 14119
VEGA: 18
HGNC: HGNC:3604
Homologene: 1515
Gucy2d
Name: guanylate cyclase 2d
Synonyms: guanylyl cyclase D
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 14918
Homologene: 71540
Or14a259
Name: olfactory receptor family 14 subfamily A member 259
Synonyms: MOR219-2, Olfr305, GA_x6K02T2NHDJ-9744055-9745014
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 258609
Homologene: 128128
Xkr9
Name: X-linked Kx blood group related 9
Synonyms: LOC381246
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 381246
Homologene: 20056
Ugt2b37
Name: UDP glucuronosyltransferase 2 family, polypeptide B37
Synonyms: 0610033E06Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 112417
Homologene: 137225
Pappa
Name: pregnancy-associated plasma protein A
Synonyms: PAPP-A, IGFBP-4ase, 8430414N03Rik, PAG1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 18491
HGNC: HGNC:8602
Homologene: 31097
Dlec1
Name: deleted in lung and esophageal cancer 1
Synonyms: D630005C06Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 320256
HGNC: HGNC:2899
Homologene: 84733
C5ar1
Name: complement component 5a receptor 1
Synonyms: C5r1, C5aR, Cd88, D7Msu1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 12273
HGNC: HGNC:1338
Homologene: 20413
A430033K04Rik
Name: RIKEN cDNA A430033K04 gene
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 243308
Homologene: 65621
Adcy6
Name: adenylate cyclase 6
Synonyms: AC6
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 11512
VEGA: 15
HGNC: HGNC:237
Homologene: 22400
Thsd7b
Name: thrombospondin, type I, domain containing 7B
Synonyms: D130067I03Rik, 1700074E13Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 210417
Homologene: 18180
Adam28
Name: a disintegrin and metallopeptidase domain 28
Synonyms: D430033C21Rik, MDC-L, Dtgn1, C130072N01Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 13522
VEGA: 14
HGNC: HGNC:206
Homologene: 40705
Ptk2b
Name: PTK2 protein tyrosine kinase 2 beta
Synonyms: Raftk, CAKbeta, cellular adhesion kinase beta, proline-rich tyrosine kinase 2, related adhesion focal tyrosine kinase, E430023O05Rik, PYK2, calcium-dependent tyrosine kinase
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 19229
HGNC: HGNC:9612
Homologene: 23001
P2rx1
Name: purinergic receptor P2X, ligand-gated ion channel, 1
Synonyms: RP-2, P2X1 receptor, P2x, Pdcd3
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 18436
HGNC: HGNC:8533
Homologene: 1921
Nanog
Name: Nanog homeobox
Synonyms: ecat4, ENK, 2410002E02Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 71950
Homologene: 78027
Slc2a13
Name: solute carrier family 2 (facilitated glucose transporter), member 13
Synonyms: A630029G22Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 239606
Homologene: 43139
4921517D22Rik
Name: RIKEN cDNA 4921517D22 gene
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 70900
VEGA: 13
Homologene: 87278
Lyset
Name: lysosomal enzyme trafficking factor
Synonyms: Tmem251, D230037D09Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 320351
VEGA: 12
Homologene: 100948
Abcb1a
Name: ATP-binding cassette, sub-family B member 1A
Synonyms: P-glycoprotein, mdr-3, Mdr1a, Pgy3, MDR3, Pgp, Pgy-3, Evi32, multiple drug resistant 1a, P-gp
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 18671
HGNC: HGNC:40
Homologene: 55496
Rnf148
Name: ring finger protein 148
Synonyms: 4933432M07Rik, Greul3
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 71300
Homologene: 82404
Otud4
Name: OTU domain containing 4
Synonyms: D8Ertd69e, 4930431L18Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 73945
Homologene: 35370
Tst
Name: thiosulfate sulfurtransferase, mitochondrial
Synonyms: Rhodanese
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 22117
VEGA: 15
Homologene: 37759
Vmn2r23
Name: vomeronasal 2, receptor 23
Synonyms: EG435916
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 435916
Homologene: 135826
Dusp13b
Name: dual specificity phosphatase 13B
Synonyms: LOC382853, Dusp13, TS-DSP6, LMW-DSP6, TMDP
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 27389
Homologene: 121602
G6pc3
Name: glucose 6 phosphatase, catalytic, 3
Synonyms: 0710001K01Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 68401
Homologene: 16304
Mfsd13b
Name: major facilitator superfamily domain containing 13B
Synonyms: 4933427G17Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 74466
Homologene: 62261
Serpinb2
Name: serine (or cysteine) peptidase inhibitor, clade B, member 2
Synonyms: PAI-2, ovalbumin, Planh2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 18788
HGNC: HGNC:8584
Homologene: 20571
Pax8
Name: paired box 8
Synonyms: Pax-8
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 18510
HGNC: HGNC:8622
Homologene: 2589
Sec11c
Name: SEC11 homolog C, signal peptidase complex subunit
Synonyms: Sec11l3, 1810029G24Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 66286
Homologene: 8624
Fbxo27
Name: F-box protein 27
Synonyms: FBG5, LOC233040, E130008B10Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233040
Homologene: 135640
Or12j5
Name: olfactory receptor family 12 subfamily J member 5
Synonyms: Olfr536, MOR252-4, MOR252-1, GA_x6K02T2PBJ9-42653501-42652578
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 258513
Homologene: 51731
Mydgf
Name: myeloid derived growth factor
Synonyms: D17Wsu104e
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 28106
Homologene: 10425
Sord
Name: sorbitol dehydrogenase
Synonyms: Sdh1, Sodh-1, Sdh-1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20322
Homologene: 56080
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 13,701,379 bp
  • T to C, chromosome 1 at 40,105,339 bp
  • T to C, chromosome 1 at 75,549,862 bp
  • T to A, chromosome 1 at 107,523,180 bp
  • T to A, chromosome 1 at 129,921,420 bp
  • T to A, chromosome 1 at 181,653,638 bp
  • T to C, chromosome 1 at 191,582,491 bp
  • A to G, chromosome 2 at 13,308,520 bp
  • A to T, chromosome 2 at 24,443,022 bp
  • C to A, chromosome 2 at 31,354,714 bp
  • G to A, chromosome 2 at 122,256,942 bp
  • G to A, chromosome 4 at 65,335,764 bp
  • T to A, chromosome 4 at 147,756,211 bp
  • T to C, chromosome 5 at 8,685,346 bp
  • C to T, chromosome 5 at 87,240,855 bp
  • T to A, chromosome 5 at 138,646,706 bp
  • A to G, chromosome 6 at 23,654,170 bp
  • A to T, chromosome 6 at 122,713,516 bp
  • A to T, chromosome 6 at 123,741,656 bp
  • A to G, chromosome 7 at 16,248,151 bp
  • A to G, chromosome 7 at 28,693,342 bp
  • A to T, chromosome 7 at 86,364,263 bp
  • G to A, chromosome 7 at 98,472,306 bp
  • T to G, chromosome 7 at 120,991,363 bp
  • A to C, chromosome 7 at 137,201,124 bp
  • T to A, chromosome 7 at 140,504,402 bp
  • C to A, chromosome 8 at 12,880,089 bp
  • T to A, chromosome 8 at 79,659,267 bp
  • T to A, chromosome 9 at 83,857,327 bp
  • A to G, chromosome 9 at 95,502,554 bp
  • T to A, chromosome 9 at 103,308,977 bp
  • T to C, chromosome 9 at 119,112,209 bp
  • A to T, chromosome 10 at 12,670,186 bp
  • G to A, chromosome 10 at 79,542,429 bp
  • T to C, chromosome 10 at 128,389,490 bp
  • A to T, chromosome 11 at 73,008,953 bp
  • A to G, chromosome 11 at 102,193,108 bp
  • T to A, chromosome 12 at 102,744,172 bp
  • A to T, chromosome 13 at 59,690,609 bp
  • G to A, chromosome 13 at 92,754,284 bp
  • C to A, chromosome 14 at 21,743,470 bp
  • T to A, chromosome 14 at 66,174,755 bp
  • C to T, chromosome 14 at 68,642,083 bp
  • T to C, chromosome 15 at 78,405,253 bp
  • T to A, chromosome 15 at 91,572,902 bp
  • A to G, chromosome 15 at 98,604,160 bp
  • C to A, chromosome 17 at 56,183,734 bp
  • G to A, chromosome 18 at 20,579,451 bp
  • A to T, chromosome 18 at 58,038,198 bp
  • A to G, chromosome 18 at 63,987,160 bp
  • A to T, chromosome 18 at 65,814,840 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8520 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067947-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.