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Strain Name:
B6J(AU)-Tg(Map1a)56Pjn/Mmjax
Stock Number:
068488-JAX
Citation ID:
RRID:MMRRC_068488-JAX

Strain Information

Tg(Map1a)56Pjn
Name: transgene insertion 56, Patsy Nishina
Synonyms: Tg(Mtap1a)56Pjn
Type: Allele
Species: Mus musculus (mouse)
Chromosome: Unknown
Alteration at locus: Transgenic
Map1a
Name: microtubule-associated protein 1 A
Synonyms: Mtap-1, Mtap1, 6330416M19Rik, Mtap1a
Type: Gene
Species: Mouse
Chromosome: 2
Alteration at locus: Transgenic
NCBI: 17754
HGNC: HGNC:6835
Homologene: 1778
Genetic Alterations
Randomly inserted mouse Map1a transgene (129P2/OlaHsd-derived) including approx. 20 kb of both, the 5' regulatory sequence and 3' downstream sequence.
ES Cell Line
Not applicable
Phenotype
This allele confers resistance to photoreceptor loss in Tulp1 KO and tub retinas as well as hearing loss in tub.
MeSH Terms
  • Adaptor Proteins, Signal Transducing
  • Alleles
  • Animals
  • Cell Line
  • Cloning, Molecular
  • DNA, Complementary/metabolism
  • Disks Large Homolog 4 Protein
  • Gene Library
  • Genetic Markers
  • Guanylate Kinases
  • Immunoblotting
  • Insulin/metabolism
  • Intracellular Signaling Peptides and Proteins
  • Membrane Proteins
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Microscopy, Fluorescence
  • Microtubule-Associated Proteins/genetics
  • Microtubule-Associated Proteins/metabolism
  • Microtubule-Associated Proteins/physiology
  • Models, Genetic
  • Nerve Tissue Proteins/genetics
  • Nerve Tissue Proteins/metabolism
  • Nerve Tissue Proteins/physiology
  • Neurons/metabolism
  • Phenotype
  • Polymorphism, Genetic
  • Precipitin Tests
  • Protein Binding
  • Protein Structure, Tertiary
  • Proteins/genetics
  • Quantitative Trait, Heritable
  • Reverse Transcriptase Polymerase Chain Reaction
  • Signal Transduction
  • Synapses/metabolism
  • Transcription, Genetic
  • Transgenes
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
  • Models for Human Disease
  • Sensorineural
Donor
Patsy Nishina, Ph.D., Jackson Laboratories.
Primary Reference

Ikeda A, Zheng QY, Zuberi AR, Johnson KR, Naggert JK, Nishina PM. Microtubule-associated protein 1A is a modifier of tubby hearing (moth1). Nat Genet. 2002 Apr;30(4):401-5. doi: 10.1038/ng838. Epub 2002 Feb 4. (Medline PMID: 11925566)

Strain Development

The Mtap1a transgenic construct contains a 129P2/OlaHsd-derived Map1a sequence and approximately 20 kb each of both the 5-prime regulatory sequence and 3-prime downstream sequence. The linearized construct was microinjected into the pronuclei of C57BL/6J embryos. Founder 59 was established and bred to B6(AU)-Tubtub/JPjn. The Tubtub was later bred away from this subline and maintained on the C57BL/6J background prior to freezing hemizygous sperm in 2018. Upon donating one vial of frozen sperm to The Jackson Laboratory Repository in 2024, mice were recovered with C57BL/6J to establish our live colony. Following at least one additional breeding to C57BL/6J, a full cohort of sperm was cryopreserved.

In 2024, the Mtap1a transgene was found to segregate along with a neo sequence. Additional details are below.
In 2024, quality control screening at The Jackson Laboratory revealed that, following two generations of additional breeding to C57BL/6J inbred mice, the RRID:MMRRC_068488-JAX hemizygotes genotyped positive for a neomycin resistance (neo) sequence, while their noncarrier (wildtype) littermates genotyped negative for neo. This indicates the Mtap1a transgene contains (or is closely linked to) a neo cassette. It has not been determined if presence of the neo has any adverse effect on expression of the Mtap1a transgene.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

For more information about this colony's health status contact csmmrrc@jax.org
Coat Color
Black
Eye
Black
MMRRC Breeding System
Sib-mating
Generation
F?
Overall Breeding Performance
Undetermined
NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Hetero/Hemizygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
Undetermined
Recommended wean age
4 Weeks
Average Pups Weaned
Undetermined

Order Request Information

Limited quantities of breeder mice (up to 2 males and 2 females or 4 mice) per investigator per month are available from a live colony, usually available to ship in under 12 weeks. Larger quantities may be available, please contact the distributing center directly at csmmrrc@jax.org for more details.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068488-JAX-HET-F
068488-JAX-HET-M
068488-JAX-WT-F
068488-JAX-WT-M
Heterozygous / Hemizygous Female
Heterozygous / Hemizygous Male
Wild Type Female
Wild Type Male
$229.00 / Non-Profit Per Mouse The csmmrrc@jax.org may assess additional fees for any special requests (e.g., specific age or weight of mice, etc.).
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.