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Strain Name:
C57BL/6J-MtgxR8713Btlr/Mmmh
Stock Number:
068567-MU
Citation ID:
RRID:MMRRC_068567-MU
Other Names:
R8713 (G1)
Major Collection:

Strain Information

Top1
Name: topoisomerase (DNA) I
Synonyms: Top-1, D130064I21Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 21969
Homologene: 2467
Ywhah
Name: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta polypeptide
Synonyms: 14-3-3 eta
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 22629
Homologene: 37767
Sec24d
Name: SEC24 homolog D, COPII coat complex component
Synonyms: 2310020L09Rik, LOC383951
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 69608
Homologene: 40986
Lrch4
Name: leucine-rich repeats and calponin homology (CH) domain containing 4
Synonyms: 2900069C24Rik, LRRN4, LRN, 2810008P14Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231798
HGNC: HGNC:6691
Homologene: 20532
Rrm1
Name: ribonucleotide reductase M1
Synonyms: RnrM1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 20133
Homologene: 806
Syne1
Name: spectrin repeat containing, nuclear envelope 1
Synonyms: nesprin-1, SYNE-1, enaptin165, A330049M09Rik, C130039F11Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 64009
VEGA: 10
Homologene: 52329
Trio
Name: triple functional domain (PTPRF interacting)
Synonyms: 6720464I07Rik, Solo
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 223435
VEGA: 15
Homologene: 20847
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 66,414,622 bp
  • C to T, chromosome 1 at 79,777,991 bp
  • T to C, chromosome 1 at 185,291,418 bp
  • G to A, chromosome 2 at 82,981,109 bp
  • A to G, chromosome 2 at 112,251,322 bp
  • G to A, chromosome 2 at 160,717,440 bp
  • T to C, chromosome 3 at 107,311,585 bp
  • T to A, chromosome 3 at 123,343,892 bp
  • A to T, chromosome 4 at 119,404,207 bp
  • A to G, chromosome 5 at 33,027,191 bp
  • A to G, chromosome 5 at 33,762,505 bp
  • C to T, chromosome 5 at 35,873,174 bp
  • T to C, chromosome 5 at 124,116,762 bp
  • G to T, chromosome 5 at 137,639,863 bp
  • T to C, chromosome 5 at 137,799,532 bp
  • A to T, chromosome 6 at 123,703,032 bp
  • C to T, chromosome 7 at 4,969,992 bp
  • C to T, chromosome 7 at 98,707,076 bp
  • T to G, chromosome 7 at 102,460,351 bp
  • T to A, chromosome 7 at 140,137,775 bp
  • T to C, chromosome 7 at 140,140,223 bp
  • A to G, chromosome 8 at 11,628,491 bp
  • T to A, chromosome 8 at 24,652,173 bp
  • C to T, chromosome 8 at 71,485,077 bp
  • A to G, chromosome 8 at 124,820,335 bp
  • T to G, chromosome 8 at 125,818,786 bp
  • G to A, chromosome 9 at 7,141,008 bp
  • A to G, chromosome 9 at 8,900,817 bp
  • A to G, chromosome 9 at 37,652,659 bp
  • A to G, chromosome 9 at 108,829,863 bp
  • A to C, chromosome 9 at 109,555,599 bp
  • A to G, chromosome 10 at 5,316,040 bp
  • T to A, chromosome 11 at 59,135,966 bp
  • C to A, chromosome 11 at 69,915,354 bp
  • T to C, chromosome 11 at 118,088,202 bp
  • T to C, chromosome 12 at 119,443,526 bp
  • T to C, chromosome 13 at 20,274,621 bp
  • A to G, chromosome 13 at 38,168,725 bp
  • A to G, chromosome 13 at 52,968,011 bp
  • C to A, chromosome 13 at 73,700,621 bp
  • G to A, chromosome 13 at 76,641,803 bp
  • A to T, chromosome 14 at 75,962,926 bp
  • A to C, chromosome 15 at 27,743,951 bp
  • G to T, chromosome 15 at 83,610,270 bp
  • A to G, chromosome 15 at 102,133,485 bp
  • A to G, chromosome 17 at 43,625,019 bp
  • A to G, chromosome 17 at 80,533,863 bp
  • T to G, chromosome 18 at 75,056,738 bp
  • C to T, chromosome 19 at 4,136,160 bp
  • T to C, chromosome 19 at 7,057,126 bp
  • T to A, chromosome 19 at 34,647,638 bp
  • T to C, chromosome 19 at 38,524,901 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8713 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068567-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.