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Strain Name:
C57BL/6J-MtgxR8790Btlr/Mmmh
Stock Number:
068634-MU
Citation ID:
RRID:MMRRC_068634-MU
Other Names:
R8790 (G1)
Major Collection:

Strain Information

Cdc25a
Name: cell division cycle 25A
Synonyms: D9Ertd393e
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 12530
HGNC: HGNC:1725
Homologene: 1355
Bmp7
Name: bone morphogenetic protein 7
Synonyms: osteogenic protein 1, OP1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 12162
HGNC: HGNC:1074
Homologene: 20410
Lgi1
Name: leucine-rich repeat LGI family, member 1
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 56839
HGNC: HGNC:6572
Homologene: 3737
Epb41l1
Name: erythrocyte membrane protein band 4.1 like 1
Synonyms: 4.1N, Epb4.1l1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 13821
HGNC: HGNC:3378
Homologene: 8126
Mthfr
Name: methylenetetrahydrofolate reductase
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 17769
HGNC: HGNC:7436
Homologene: 4349
Tmed10
Name: transmembrane p24 trafficking protein 10
Synonyms: 1110014C03Rik, p24delta1, p23
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 68581
VEGA: 12
Homologene: 4972
Ubap2
Name: ubiquitin-associated protein 2
Synonyms: 1190005K07Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 68926
Homologene: 73649
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 66,438,838 bp
  • T to C, chromosome 1 at 87,477,597 bp
  • T to A, chromosome 1 at 131,755,417 bp
  • T to C, chromosome 2 at 86,593,934 bp
  • A to T, chromosome 2 at 129,269,252 bp
  • C to T, chromosome 2 at 151,970,418 bp
  • T to G, chromosome 2 at 156,503,802 bp
  • C to T, chromosome 2 at 167,050,580 bp
  • T to A, chromosome 2 at 172,870,267 bp
  • G to T, chromosome 2 at 181,187,853 bp
  • T to A, chromosome 3 at 19,546,817 bp
  • T to C, chromosome 3 at 95,168,030 bp
  • A to G, chromosome 4 at 41,209,351 bp
  • T to A, chromosome 4 at 58,118,145 bp
  • T to A, chromosome 4 at 118,581,914 bp
  • T to A, chromosome 4 at 142,037,745 bp
  • T to A, chromosome 4 at 144,159,140 bp
  • C to A, chromosome 4 at 148,055,534 bp
  • A to G, chromosome 4 at 150,508,875 bp
  • G to T, chromosome 5 at 27,772,080 bp
  • A to G, chromosome 5 at 53,699,949 bp
  • A to G, chromosome 5 at 86,707,400 bp
  • C to T, chromosome 5 at 96,755,377 bp
  • A to G, chromosome 5 at 124,077,241 bp
  • T to C, chromosome 5 at 124,315,673 bp
  • T to A, chromosome 6 at 29,535,027 bp
  • A to G, chromosome 6 at 41,047,721 bp
  • G to T, chromosome 6 at 133,107,885 bp
  • A to T, chromosome 7 at 14,332,983 bp
  • A to G, chromosome 7 at 26,198,742 bp
  • A to T, chromosome 7 at 29,076,872 bp
  • G to A, chromosome 7 at 30,764,024 bp
  • G to A, chromosome 7 at 79,793,077 bp
  • A to T, chromosome 7 at 128,252,877 bp
  • A to T, chromosome 8 at 13,677,391 bp
  • T to G, chromosome 8 at 15,119,242 bp
  • T to C, chromosome 8 at 125,877,567 bp
  • A to T, chromosome 9 at 37,531,631 bp
  • T to A, chromosome 9 at 39,750,908 bp
  • C to A, chromosome 9 at 51,147,840 bp
  • T to A, chromosome 9 at 109,887,348 bp
  • G to A, chromosome 10 at 79,092,451 bp
  • G to A, chromosome 10 at 121,667,663 bp
  • T to G, chromosome 10 at 127,457,652 bp
  • G to A, chromosome 10 at 127,539,077 bp
  • A to G, chromosome 11 at 8,518,273 bp
  • G to T, chromosome 11 at 50,832,602 bp
  • A to C, chromosome 11 at 60,476,536 bp
  • G to T, chromosome 11 at 60,487,221 bp
  • G to A, chromosome 11 at 60,740,537 bp
  • G to T, chromosome 11 at 61,147,524 bp
  • T to G, chromosome 11 at 106,312,047 bp
  • A to G, chromosome 11 at 120,132,693 bp
  • A to T, chromosome 12 at 13,223,992 bp
  • T to C, chromosome 12 at 69,197,294 bp
  • T to C, chromosome 12 at 70,021,019 bp
  • T to C, chromosome 12 at 83,777,144 bp
  • C to A, chromosome 12 at 85,343,480 bp
  • T to A, chromosome 12 at 118,867,885 bp
  • C to T, chromosome 13 at 20,851,670 bp
  • C to T, chromosome 13 at 34,060,485 bp
  • C to T, chromosome 13 at 100,506,219 bp
  • A to T, chromosome 14 at 31,296,275 bp
  • A to G, chromosome 14 at 49,180,751 bp
  • T to A, chromosome 14 at 53,470,220 bp
  • A to T, chromosome 14 at 54,186,130 bp
  • T to A, chromosome 15 at 75,747,375 bp
  • C to T, chromosome 15 at 84,118,618 bp
  • T to A, chromosome 15 at 98,599,000 bp
  • T to C, chromosome 15 at 99,943,143 bp
  • T to A, chromosome 16 at 3,917,923 bp
  • A to T, chromosome 16 at 56,675,074 bp
  • T to C, chromosome 16 at 59,036,217 bp
  • T to A, chromosome 16 at 84,809,371 bp
  • T to A, chromosome 17 at 18,940,210 bp
  • A to G, chromosome 17 at 46,680,700 bp
  • A to T, chromosome 17 at 65,983,158 bp
  • A to G, chromosome 17 at 75,547,689 bp
  • C to A, chromosome 18 at 5,056,098 bp
  • C to A, chromosome 19 at 4,732,024 bp
  • A to T, chromosome 19 at 5,685,178 bp
  • T to A, chromosome 19 at 7,268,975 bp
  • T to C, chromosome 19 at 12,120,542 bp
  • T to C, chromosome 19 at 27,416,799 bp
  • C to T, chromosome 19 at 38,300,848 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8790 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068634-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.