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Strain Name:
C57BL/6J-MtgxR8812Btlr/Mmmh
Stock Number:
068647-MU
Citation ID:
RRID:MMRRC_068647-MU
Other Names:
R8812 (G1)
Major Collection:

Strain Information

Tln2
Name: talin 2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 70549
VEGA: 9
Homologene: 56692
Sbf2
Name: SET binding factor 2
Synonyms: SBF2, 4833411B01Rik, mMTMH1, B430219L04Rik, Mtmr13
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 319934
HGNC: HGNC:2135
Homologene: 41810
Slc26a5
Name: solute carrier family 26, member 5
Synonyms: Pres, prestin
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 80979
HGNC: HGNC:9359
Homologene: 69472
Pmp22
Name: peripheral myelin protein 22
Synonyms: Gas-3, TRE002
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 18858
HGNC: HGNC:9118
Homologene: 7482
Bpifb3
Name: BPI fold containing family B, member 3
Synonyms: Rya3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 378700
Homologene: 18807
Adcy10
Name: adenylate cyclase 10
Synonyms: soluble adenylyl cyclase, sAC, 4930431D04Rik, Sacy
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 271639
Homologene: 10188
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 37,909,973 bp
  • A to G, chromosome 1 at 58,994,319 bp
  • C to A, chromosome 1 at 63,308,113 bp
  • A to T, chromosome 1 at 165,551,298 bp
  • T to A, chromosome 2 at 28,451,180 bp
  • T to C, chromosome 2 at 28,840,804 bp
  • T to A, chromosome 2 at 31,284,201 bp
  • T to C, chromosome 2 at 32,364,274 bp
  • A to G, chromosome 2 at 89,439,865 bp
  • G to T, chromosome 2 at 89,978,371 bp
  • T to A, chromosome 2 at 150,267,704 bp
  • C to T, chromosome 2 at 153,922,596 bp
  • T to A, chromosome 3 at 30,679,179 bp
  • T to A, chromosome 3 at 51,266,767 bp
  • T to C, chromosome 3 at 79,908,771 bp
  • G to A, chromosome 3 at 88,895,007 bp
  • T to A, chromosome 3 at 95,356,060 bp
  • A to G, chromosome 4 at 11,202,279 bp
  • A to T, chromosome 4 at 65,204,929 bp
  • C to A, chromosome 4 at 112,988,952 bp
  • A to G, chromosome 4 at 126,745,112 bp
  • T to C, chromosome 5 at 3,631,614 bp
  • T to A, chromosome 5 at 21,813,882 bp
  • T to C, chromosome 5 at 87,587,642 bp
  • A to T, chromosome 5 at 90,293,203 bp
  • T to C, chromosome 5 at 93,637,801 bp
  • T to G, chromosome 6 at 35,214,334 bp
  • A to C, chromosome 6 at 38,636,903 bp
  • T to A, chromosome 6 at 41,462,586 bp
  • A to G, chromosome 6 at 57,258,138 bp
  • A to G, chromosome 6 at 57,404,451 bp
  • A to T, chromosome 6 at 86,676,214 bp
  • C to T, chromosome 6 at 132,711,739 bp
  • T to A, chromosome 6 at 134,456,178 bp
  • A to T, chromosome 6 at 148,775,077 bp
  • T to G, chromosome 7 at 4,664,784 bp
  • A to G, chromosome 7 at 7,474,670 bp
  • A to G, chromosome 7 at 11,880,703 bp
  • A to G, chromosome 7 at 47,981,733 bp
  • A to G, chromosome 7 at 85,005,685 bp
  • G to T, chromosome 7 at 103,560,609 bp
  • T to A, chromosome 7 at 107,793,255 bp
  • A to T, chromosome 7 at 107,794,044 bp
  • A to T, chromosome 7 at 110,329,862 bp
  • G to T, chromosome 7 at 116,351,877 bp
  • A to C, chromosome 7 at 125,797,695 bp
  • C to T, chromosome 7 at 128,133,807 bp
  • T to C, chromosome 8 at 125,669,925 bp
  • A to G, chromosome 9 at 18,988,516 bp
  • G to T, chromosome 9 at 46,178,513 bp
  • T to C, chromosome 9 at 59,779,747 bp
  • T to A, chromosome 9 at 64,928,703 bp
  • C to A, chromosome 9 at 67,221,411 bp
  • T to A, chromosome 9 at 121,750,251 bp
  • A to G, chromosome 10 at 85,627,249 bp
  • T to C, chromosome 11 at 59,035,095 bp
  • T to A, chromosome 11 at 63,158,413 bp
  • G to A, chromosome 11 at 67,209,141 bp
  • T to A, chromosome 11 at 74,317,708 bp
  • T to C, chromosome 11 at 79,546,354 bp
  • T to C, chromosome 11 at 85,559,147 bp
  • C to T, chromosome 12 at 30,890,056 bp
  • G to T, chromosome 12 at 84,996,792 bp
  • A to G, chromosome 12 at 86,488,550 bp
  • A to T, chromosome 12 at 115,386,747 bp
  • T to C, chromosome 13 at 21,073,050 bp
  • C to A, chromosome 13 at 77,332,352 bp
  • T to A, chromosome 13 at 99,432,815 bp
  • A to G, chromosome 14 at 7,887,624 bp
  • C to T, chromosome 14 at 50,837,132 bp
  • C to A, chromosome 14 at 61,310,973 bp
  • A to T, chromosome 15 at 27,905,225 bp
  • G to A, chromosome 15 at 39,084,471 bp
  • T to C, chromosome 15 at 89,283,072 bp
  • T to A, chromosome 16 at 25,982,717 bp
  • T to C, chromosome 17 at 24,960,604 bp
  • A to T, chromosome 17 at 71,800,805 bp
  • T to A, chromosome 17 at 80,127,786 bp
  • T to C, chromosome 18 at 37,007,663 bp
  • G to A, chromosome 19 at 10,026,777 bp
  • A to T, chromosome 19 at 12,890,196 bp
  • G to A, chromosome X at 150,655,559 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8812 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068647-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.