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Strain Detail Sheet

Strain Name:
C57BL/6J-MtgxR8927Btlr/Mmmh
Stock Number:
068771-MU
Citation ID:
RRID:MMRRC_068771-MU
Other Names:
R8927 (G1)
Major Collection:
Beutler Mutagenetix

Strain Information

Gene Details

Gnb5
Name: guanine nucleotide binding protein (G protein), beta 5
Synonyms: G beta 5, Gbeta5
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 14697
VEGA: 9
HGNC: HGNC:4401
Homologene: 40714

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Rptor
Name: regulatory associated protein of MTOR, complex 1
Synonyms: raptor, 4932417H02Rik, Rap
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 74370
Homologene: 80210

Additional Resources
IMPC
Google
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PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
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ClinGen
Vps41
Name: VPS41 HOPS complex subunit
Synonyms: Vam2, mVam2
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 218035
VEGA: 13
Homologene: 69165

Additional Resources
IMPC
Google
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PubMed
IMSR
Gene Ontology
BioGPS
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Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
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ClinGen
Fn1
Name: fibronectin 1
Synonyms: Fn-1, Fn
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 14268
HGNC: HGNC:3778
Homologene: 1533

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
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KOMP Phenotyping
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ClinGen
Sptbn1
Name: spectrin beta, non-erythrocytic 1
Synonyms: beta fodrin, Spnb-2, spectrin G, brain spectrin, elf3, elf1, 9930031C03Rik, non-erythrocytic, Spnb2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 20742
Homologene: 2354

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Notch3
Name: notch 3
Synonyms: hpbk, N3
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 18131
HGNC: HGNC:7883
Homologene: 376

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Bmp7
Name: bone morphogenetic protein 7
Synonyms: osteogenic protein 1, OP1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 12162
HGNC: HGNC:1074
Homologene: 20410

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 43,033,154 bp
  • T to C, chromosome 1 at 71,599,376 bp
  • T to A, chromosome 1 at 86,305,117 bp
  • A to G, chromosome 1 at 92,608,716 bp
  • T to A, chromosome 1 at 132,328,178 bp
  • T to A, chromosome 1 at 135,978,246 bp
  • T to A, chromosome 1 at 139,490,387 bp
  • T to A, chromosome 1 at 140,428,797 bp
  • T to C, chromosome 1 at 162,876,829 bp
  • T to A, chromosome 1 at 172,104,170 bp
  • T to G, chromosome 1 at 181,680,756 bp
  • A to G, chromosome 2 at 29,126,959 bp
  • A to T, chromosome 2 at 32,367,500 bp
  • G to A, chromosome 2 at 33,242,304 bp
  • A to T, chromosome 2 at 85,849,574 bp
  • T to C, chromosome 2 at 86,347,746 bp
  • T to C, chromosome 2 at 86,677,286 bp
  • C to A, chromosome 2 at 104,992,197 bp
  • A to T, chromosome 2 at 111,754,762 bp
  • T to A, chromosome 2 at 114,050,400 bp
  • A to G, chromosome 2 at 129,040,869 bp
  • T to A, chromosome 2 at 130,737,380 bp
  • T to A, chromosome 2 at 165,123,584 bp
  • C to T, chromosome 2 at 166,585,075 bp
  • C to T, chromosome 2 at 172,879,418 bp
  • T to C, chromosome 3 at 52,345,282 bp
  • G to T, chromosome 3 at 86,831,741 bp
  • G to T, chromosome 3 at 87,754,583 bp
  • C to T, chromosome 3 at 90,220,813 bp
  • T to A, chromosome 3 at 93,692,643 bp
  • A to T, chromosome 3 at 95,138,520 bp
  • T to G, chromosome 3 at 108,386,657 bp
  • T to C, chromosome 3 at 110,250,932 bp
  • T to C, chromosome 3 at 117,575,883 bp
  • T to A, chromosome 3 at 122,839,600 bp
  • A to T, chromosome 4 at 42,972,251 bp
  • T to C, chromosome 4 at 58,301,638 bp
  • A to T, chromosome 4 at 64,007,358 bp
  • T to A, chromosome 4 at 106,746,045 bp
  • G to A, chromosome 4 at 113,483,902 bp
  • C to T, chromosome 4 at 123,208,488 bp
  • G to T, chromosome 4 at 129,461,646 bp
  • A to T, chromosome 4 at 149,453,501 bp
  • T to C, chromosome 5 at 21,333,062 bp
  • A to T, chromosome 5 at 25,971,349 bp
  • T to A, chromosome 5 at 26,021,680 bp
  • A to G, chromosome 5 at 31,299,559 bp
  • G to T, chromosome 5 at 34,966,289 bp
  • T to C, chromosome 5 at 108,802,265 bp
  • T to C, chromosome 5 at 124,083,643 bp
  • A to T, chromosome 5 at 135,249,735 bp
  • C to T, chromosome 5 at 137,298,808 bp
  • A to G, chromosome 6 at 29,406,110 bp
  • T to A, chromosome 6 at 32,804,016 bp
  • T to C, chromosome 6 at 43,174,735 bp
  • A to T, chromosome 6 at 47,533,779 bp
  • A to G, chromosome 6 at 57,754,593 bp
  • C to A, chromosome 6 at 65,381,613 bp
  • T to A, chromosome 6 at 121,007,364 bp
  • T to A, chromosome 6 at 122,969,369 bp
  • T to G, chromosome 6 at 135,772,341 bp
  • T to A, chromosome 6 at 142,354,711 bp
  • G to A, chromosome 7 at 3,489,203 bp
  • A to C, chromosome 7 at 3,611,748 bp
  • A to C, chromosome 7 at 3,739,359 bp
  • G to A, chromosome 7 at 16,273,055 bp
  • A to G, chromosome 7 at 23,606,389 bp
  • T to C, chromosome 7 at 31,705,177 bp
  • C to A, chromosome 7 at 38,221,574 bp
  • A to G, chromosome 7 at 43,466,596 bp
  • G to A, chromosome 7 at 43,954,782 bp
  • T to C, chromosome 7 at 99,169,503 bp
  • T to G, chromosome 7 at 99,471,014 bp
  • C to T, chromosome 7 at 104,232,448 bp
  • A to T, chromosome 7 at 104,565,022 bp
  • C to A, chromosome 7 at 105,734,866 bp
  • T to C, chromosome 7 at 114,030,357 bp
  • A to T, chromosome 7 at 122,124,598 bp
  • A to G, chromosome 8 at 11,425,543 bp
  • T to G, chromosome 8 at 36,147,206 bp
  • T to A, chromosome 8 at 45,795,915 bp
  • C to T, chromosome 8 at 85,840,791 bp
  • T to G, chromosome 8 at 105,688,498 bp
  • T to C, chromosome 8 at 125,887,920 bp
  • T to C, chromosome 9 at 19,641,225 bp
  • T to G, chromosome 9 at 57,141,172 bp
  • G to A, chromosome 9 at 57,258,522 bp
  • T to C, chromosome 9 at 75,344,954 bp
  • A to G, chromosome 9 at 97,100,073 bp
  • C to A, chromosome 9 at 100,705,245 bp
  • C to T, chromosome 9 at 108,294,509 bp
  • A to T, chromosome 10 at 26,344,186 bp
  • G to A, chromosome 10 at 77,742,328 bp
  • T to C, chromosome 10 at 78,752,424 bp
  • T to A, chromosome 10 at 81,293,072 bp
  • T to A, chromosome 10 at 92,722,396 bp
  • A to T, chromosome 10 at 126,887,493 bp
  • T to A, chromosome 11 at 3,529,477 bp
  • C to T, chromosome 11 at 30,138,962 bp
  • T to C, chromosome 11 at 54,059,964 bp
  • T to A, chromosome 11 at 59,086,869 bp
  • A to T, chromosome 11 at 67,188,683 bp
  • G to A, chromosome 11 at 67,283,255 bp
  • A to T, chromosome 11 at 73,847,580 bp
  • C to T, chromosome 11 at 91,577,197 bp
  • A to C, chromosome 11 at 101,667,066 bp
  • A to G, chromosome 11 at 103,377,213 bp
  • A to G, chromosome 11 at 105,325,508 bp
  • A to G, chromosome 11 at 105,810,505 bp
  • A to G, chromosome 11 at 106,051,926 bp
  • C to T, chromosome 11 at 119,891,210 bp
  • T to C, chromosome 12 at 100,966,417 bp
  • T to A, chromosome 13 at 18,745,331 bp
  • T to C, chromosome 13 at 21,735,355 bp
  • T to C, chromosome 13 at 22,102,641 bp
  • T to G, chromosome 13 at 110,399,216 bp
  • T to C, chromosome 13 at 114,968,519 bp
  • A to G, chromosome 13 at 118,386,669 bp
  • A to T, chromosome 14 at 24,156,479 bp
  • T to A, chromosome 15 at 10,530,634 bp
  • G to A, chromosome 15 at 58,009,917 bp
  • AGCTGGGGTCCCGC to AGC, chromosome 15 at 89,185,577 bp
  • A to G, chromosome 15 at 98,438,638 bp
  • T to C, chromosome 15 at 103,444,356 bp
  • T to C, chromosome 16 at 4,551,354 bp
  • T to A, chromosome 16 at 5,087,610 bp
  • T to G, chromosome 16 at 55,849,634 bp
  • A to G, chromosome 16 at 57,171,789 bp
  • A to T, chromosome 16 at 98,066,167 bp
  • T to C, chromosome 17 at 17,893,462 bp
  • A to G, chromosome 17 at 18,326,238 bp
  • C to T, chromosome 17 at 32,153,818 bp
  • C to A, chromosome 17 at 45,410,913 bp
  • A to T, chromosome 17 at 45,664,982 bp
  • T to C, chromosome 17 at 46,549,169 bp
  • A to T, chromosome 17 at 66,448,633 bp
  • A to G, chromosome 17 at 68,841,738 bp
  • T to C, chromosome 17 at 73,324,766 bp
  • A to T, chromosome 18 at 19,974,177 bp
  • T to C, chromosome 18 at 20,592,478 bp
  • G to A, chromosome 18 at 37,487,319 bp
  • A to T, chromosome 18 at 56,526,988 bp
  • T to C, chromosome 19 at 5,388,501 bp
  • A to C, chromosome 19 at 11,556,210 bp

Strain Description

Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.

Strain Origin

Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8927 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat Color
Black

Breeding

MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain

Reproductive Statistics

Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
068771-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.