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Strain Name:
C57BL/6J-MtgxR9038Btlr/Mmmh
Stock Number:
068866-MU
Citation ID:
RRID:MMRRC_068866-MU
Other Names:
R9038 (G1)
Major Collection:

Strain Information

Polr3e
Name: polymerase (RNA) III (DNA directed) polypeptide E
Synonyms: RPC5, Sin
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 26939
Homologene: 14052
Pi4k2a
Name: phosphatidylinositol 4-kinase type 2 alpha
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 84095
VEGA: 19
Homologene: 101681
Aco2
Name: aconitase 2, mitochondrial
Synonyms: Aco3, Aco-2, D10Wsu183e, Irp1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 11429
HGNC: HGNC:118
Homologene: 856
Arhgef18
Name: Rho/Rac guanine nucleotide exchange factor 18
Synonyms: D030053O22Rik, A430078G23Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 102098
Homologene: 32254
Virma
Name: vir like m6A methyltransferase associated
Synonyms: 1110037F02Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 66185
Homologene: 41043
Pan2
Name: PAN2 poly(A) specific ribonuclease subunit
Synonyms: 1200014O24Rik, Usp52
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 103135
VEGA: 10
Homologene: 5918
Sp1
Name: trans-acting transcription factor 1
Synonyms: Sp1-1, 1110003E12Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 20683
Homologene: 8276
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • GT to GTT, chromosome 1 at 88,266,524 bp
  • C to A, chromosome 1 at 135,846,746 bp
  • T to A, chromosome 1 at 173,270,580 bp
  • T to C, chromosome 2 at 26,225,234 bp
  • T to C, chromosome 2 at 33,411,236 bp
  • T to A, chromosome 2 at 66,494,803 bp
  • G to A, chromosome 2 at 75,977,871 bp
  • T to A, chromosome 2 at 76,914,712 bp
  • A to T, chromosome 2 at 86,754,010 bp
  • A to G, chromosome 2 at 87,746,781 bp
  • G to A, chromosome 2 at 130,691,743 bp
  • G to T, chromosome 2 at 140,275,464 bp
  • A to G, chromosome 2 at 150,471,944 bp
  • A to T, chromosome 2 at 155,564,874 bp
  • A to G, chromosome 3 at 99,314,769 bp
  • A to G, chromosome 3 at 135,218,036 bp
  • A to G, chromosome 4 at 11,526,922 bp
  • A to G, chromosome 4 at 121,616,419 bp
  • A to G, chromosome 5 at 24,571,193 bp
  • G to T, chromosome 5 at 31,284,055 bp
  • A to T, chromosome 5 at 96,726,883 bp
  • A to G, chromosome 5 at 112,333,348 bp
  • A to G, chromosome 6 at 87,112,251 bp
  • C to T, chromosome 7 at 5,327,479 bp
  • G to A, chromosome 7 at 6,316,544 bp
  • G to A, chromosome 7 at 28,564,838 bp
  • G to A, chromosome 7 at 73,455,610 bp
  • C to A, chromosome 7 at 84,304,851 bp
  • A to G, chromosome 7 at 92,917,809 bp
  • T to C, chromosome 7 at 105,091,226 bp
  • A to G, chromosome 7 at 118,899,658 bp
  • T to C, chromosome 7 at 120,937,683 bp
  • A to G, chromosome 8 at 3,453,257 bp
  • G to C, chromosome 8 at 70,726,901 bp
  • G to A, chromosome 8 at 90,989,605 bp
  • A to G, chromosome 9 at 62,767,757 bp
  • G to T, chromosome 9 at 80,255,003 bp
  • A to G, chromosome 9 at 90,174,639 bp
  • T to C, chromosome 10 at 28,781,753 bp
  • C to T, chromosome 10 at 128,317,941 bp
  • T to C, chromosome 10 at 129,801,695 bp
  • C to T, chromosome 11 at 86,189,773 bp
  • A to G, chromosome 12 at 75,308,926 bp
  • A to G, chromosome 12 at 85,220,123 bp
  • A to T, chromosome 12 at 118,931,916 bp
  • C to A, chromosome 13 at 47,049,294 bp
  • T to C, chromosome 13 at 56,888,073 bp
  • T to C, chromosome 13 at 62,700,193 bp
  • T to C, chromosome 14 at 51,414,033 bp
  • C to A, chromosome 14 at 51,997,615 bp
  • T to A, chromosome 14 at 52,372,259 bp
  • T to A, chromosome 14 at 55,565,657 bp
  • G to T, chromosome 15 at 35,875,785 bp
  • C to A, chromosome 15 at 75,045,451 bp
  • G to A, chromosome 15 at 75,839,388 bp
  • A to G, chromosome 15 at 78,884,121 bp
  • C to G, chromosome 15 at 81,872,419 bp
  • A to G, chromosome 15 at 101,141,130 bp
  • A to G, chromosome 15 at 102,407,885 bp
  • T to A, chromosome 17 at 18,304,209 bp
  • T to C, chromosome 17 at 25,857,226 bp
  • T to C, chromosome 17 at 36,988,530 bp
  • C to A, chromosome 17 at 45,559,257 bp
  • A to T, chromosome 17 at 55,611,649 bp
  • A to G, chromosome 18 at 23,610,496 bp
  • C to A, chromosome 18 at 33,158,900 bp
  • T to C, chromosome 19 at 6,067,142 bp
  • T to C, chromosome 19 at 10,843,502 bp
  • A to T, chromosome 19 at 29,754,500 bp
  • G to A, chromosome 19 at 42,100,796 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9038 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068866-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.