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Strain Name:
C57BL/6J-MtgxR9276Btlr/Mmmh
Stock Number:
069095-MU
Citation ID:
RRID:MMRRC_069095-MU
Other Names:
R9276 (G1)
Major Collection:

Strain Information

Ptch2
Name: patched 2
Synonyms: ptc2
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 19207
HGNC: HGNC:9586
Homologene: 37842
Pzp2
Name: PZP alpha-2-macroglobulin like 2
Synonyms: Pzp
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 11287
Homologene: 104112
Macf1
Name: microtubule-actin crosslinking factor 1
Synonyms: Acf7, Aclp7, trabeculin alpha
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11426
Homologene: 136191
Dlc1
Name: deleted in liver cancer 1
Synonyms: p122-RhoGAP, STARD12, Arhgap7, A730069N07Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 50768
HGNC: HGNC:2897
Homologene: 4442
Spidr
Name: scaffolding protein involved in DNA repair
Synonyms: 2310008H04Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 224008
VEGA: 16
Homologene: 51693
Fzd6
Name: frizzled class receptor 6
Synonyms: Fz6, rst
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 14368
VEGA: 15
HGNC: HGNC:4044
Homologene: 2617
Rbm6
Name: RNA binding motif protein 6
Synonyms: NY-LU-12, g16, Def-3
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 19654
HGNC: HGNC:9903
Homologene: 31336
Genetic Alterations
ENU-induced transitions at the following base pair locations:
  • A to C, chromosome 1 at 52,708,355 bp (GRCm38)
  • A to T, chromosome 1 at 55,360,653 bp (GRCm38)
  • G to A, chromosome 1 at 90,807,681 bp (GRCm38)
  • A to G, chromosome 1 at 170,927,566 bp (GRCm38)
  • C to T, chromosome 2 at 29,939,502 bp (GRCm38)
  • T to C, chromosome 2 at 84,375,299 bp (GRCm38)
  • A to T, chromosome 2 at 86,526,048 bp (GRCm38)
  • A to G, chromosome 2 at 88,093,462 bp (GRCm38)
  • T to C, chromosome 2 at 120,030,414 bp (GRCm38)
  • T to A, chromosome 2 at 152,628,049 bp (GRCm38)
  • T to G, chromosome 3 at 81,969,578 bp (GRCm38)
  • G to T, chromosome 3 at 87,435,831 bp (GRCm38)
  • A to G, chromosome 3 at 133,071,904 bp (GRCm38)
  • A to T, chromosome 4 at 56,806,141 bp (GRCm38)
  • T to A, chromosome 4 at 117,110,308 bp (GRCm38)
  • T to G, chromosome 4 at 123,434,708 bp (GRCm38)
  • T to A, chromosome 4 at 125,053,457 bp (GRCm38)
  • T to C, chromosome 5 at 6,771,398 bp (GRCm38)
  • T to A, chromosome 5 at 33,414,836 bp (GRCm38)
  • C to A, chromosome 5 at 125,036,086 bp (GRCm38)
  • G to A, chromosome 5 at 134,221,839 bp (GRCm38)
  • A to T, chromosome 5 at 146,160,107 bp (GRCm38)
  • T to A, chromosome 6 at 4,674,585 bp (GRCm38)
  • A to G, chromosome 6 at 89,747,098 bp (GRCm38)
  • T to C, chromosome 6 at 118,624,433 bp (GRCm38)
  • A to T, chromosome 6 at 128,522,114 bp (GRCm38)
  • A to G, chromosome 6 at 132,951,613 bp (GRCm38)
  • C to T, chromosome 7 at 6,394,441 bp (GRCm38)
  • C to A, chromosome 7 at 29,102,829 bp (GRCm38)
  • A to T, chromosome 7 at 31,360,103 bp (GRCm38)
  • G to T, chromosome 7 at 35,620,570 bp (GRCm38)
  • A to T, chromosome 7 at 65,910,202 bp (GRCm38)
  • A to G, chromosome 7 at 82,557,502 bp (GRCm38)
  • A to G, chromosome 7 at 87,037,837 bp (GRCm38)
  • A to G, chromosome 7 at 100,626,602 bp (GRCm38)
  • A to T, chromosome 7 at 103,560,797 bp (GRCm38)
  • T to A, chromosome 7 at 112,894,394 bp (GRCm38)
  • A to T, chromosome 7 at 139,621,291 bp (GRCm38)
  • A to G, chromosome 8 at 36,579,404 bp (GRCm38)
  • A to G, chromosome 8 at 45,035,477 bp (GRCm38)
  • G to T, chromosome 8 at 62,096,719 bp (GRCm38)
  • A to G, chromosome 8 at 88,325,386 bp (GRCm38)
  • C to A, chromosome 8 at 124,887,785 bp (GRCm38)
  • C to A, chromosome 9 at 14,751,276 bp (GRCm38)
  • A to T, chromosome 9 at 36,534,431 bp (GRCm38)
  • C to A, chromosome 9 at 37,898,119 bp (GRCm38)
  • A to T, chromosome 9 at 40,092,336 bp (GRCm38)
  • T to C, chromosome 9 at 88,841,913 bp (GRCm38)
  • G to A, chromosome 9 at 95,708,235 bp (GRCm38)
  • A to T, chromosome 9 at 101,942,688 bp (GRCm38)
  • A to G, chromosome 9 at 107,783,727 bp (GRCm38)
  • C to A, chromosome 9 at 108,301,211 bp (GRCm38)
  • C to T, chromosome 9 at 119,402,550 bp (GRCm38)
  • A to T, chromosome 9 at 123,856,615 bp (GRCm38)
  • A to G, chromosome 10 at 6,907,936 bp (GRCm38)
  • G to A, chromosome 10 at 23,900,896 bp (GRCm38)
  • A to G, chromosome 10 at 68,778,658 bp (GRCm38)
  • T to A, chromosome 11 at 52,383,962 bp (GRCm38)
  • T to C, chromosome 11 at 59,177,627 bp (GRCm38)
  • T to G, chromosome 12 at 40,649,405 bp (GRCm38)
  • G to A, chromosome 12 at 84,791,090 bp (GRCm38)
  • T to G, chromosome 12 at 84,830,111 bp (GRCm38)
  • T to C, chromosome 12 at 98,798,801 bp (GRCm38)
  • T to C, chromosome 12 at 112,014,501 bp (GRCm38)
  • A to G, chromosome 13 at 22,006,005 bp (GRCm38)
  • A to G, chromosome 13 at 55,718,101 bp (GRCm38)
  • T to C, chromosome 14 at 55,102,361 bp (GRCm38)
  • T to C, chromosome 14 at 56,482,097 bp (GRCm38)
  • A to G, chromosome 14 at 63,543,552 bp (GRCm38)
  • A to G, chromosome 15 at 39,007,567 bp (GRCm38)
  • A to G, chromosome 15 at 76,176,245 bp (GRCm38)
  • A to C, chromosome 15 at 81,323,613 bp (GRCm38)
  • T to C, chromosome 15 at 89,145,169 bp (GRCm38)
  • G to A, chromosome 16 at 15,966,848 bp (GRCm38)
  • T to A, chromosome 16 at 20,157,036 bp (GRCm38)
  • C to T, chromosome 16 at 43,943,678 bp (GRCm38)
  • A to G, chromosome 16 at 58,824,371 bp (GRCm38)
  • C to T, chromosome 16 at 77,113,833 bp (GRCm38)
  • C to T, chromosome 16 at 90,772,575 bp (GRCm38)
  • T to A, chromosome 17 at 13,804,008 bp (GRCm38)
  • A to T, chromosome 17 at 20,890,298 bp (GRCm38)
  • A to T, chromosome 17 at 24,186,140 bp (GRCm38)
  • A to G, chromosome 17 at 34,710,160 bp (GRCm38)
  • T to C, chromosome 17 at 35,530,208 bp (GRCm38)
  • T to C, chromosome 18 at 13,844,641 bp (GRCm38)
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9276 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069095-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.