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Strain Name:
C57BL/6J-MtgxR9422Btlr/Mmmh
Stock Number:
069226-MU
Citation ID:
RRID:MMRRC_069226-MU
Other Names:
R9422 (G1)
Major Collection:

Strain Information

Notch2
Name: notch 2
Synonyms: Motch B, N2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 18129
HGNC: HGNC:7882
Homologene: 7865
Ank2
Name: ankyrin 2, brain
Synonyms: ankyrin B, Ank-2, Ankyrin-2, Ankyrin-B, Gm4392
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 109676
HGNC: HGNC:493
Becn1
Name: beclin 1, autophagy related
Synonyms: Atg6
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 56208
HGNC: HGNC:1034
Homologene: 2794
Thop1
Name: thimet oligopeptidase 1
Synonyms: EP24.15
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 50492
Homologene: 55726
Tanc1
Name: tetratricopeptide repeat, ankyrin repeat and coiled-coil containing 1
Synonyms: 1200003E16Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 66860
Homologene: 18946
Clasp1
Name: CLIP associating protein 1
Synonyms: 5730583A19Rik, 1700030C23Rik, mCLASP1, CLASP1alpha, CLASP1, B130045P17Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 76707
Homologene: 41024
H2bc3
Name: H2B clustered histone 3
Synonyms: H2b-143, Hist1h2bb
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 319178
HGNC: HGNC:4751
Homologene: 137348
Genetic Alterations
ENU-induced transitions at the following base pair locations:
  • T to A, chromosome 1 at 43,978,737 bp (GRCm38)
  • T to A, chromosome 1 at 118,462,527 bp (GRCm38)
  • T to C, chromosome 1 at 144,003,045 bp (GRCm38)
  • T to A, chromosome 1 at 194,644,422 bp (GRCm38)
  • A to G, chromosome 2 at 28,545,172 bp (GRCm38)
  • T to C, chromosome 2 at 59,807,589 bp (GRCm38)
  • T to C, chromosome 2 at 60,252,353 bp (GRCm38)
  • G to A, chromosome 2 at 119,783,038 bp (GRCm38)
  • C to T, chromosome 2 at 160,782,100 bp (GRCm38)
  • A to G, chromosome 2 at 163,492,606 bp (GRCm38)
  • T to C, chromosome 3 at 33,082,252 bp (GRCm38)
  • A to G, chromosome 3 at 98,147,352 bp (GRCm38)
  • C to A, chromosome 3 at 127,096,856 bp (GRCm38)
  • A to T, chromosome 4 at 47,293,364 bp (GRCm38)
  • A to G, chromosome 4 at 105,051,998 bp (GRCm38)
  • T to A, chromosome 4 at 143,656,412 bp (GRCm38)
  • T to C, chromosome 4 at 148,164,159 bp (GRCm38)
  • A to G, chromosome 5 at 5,649,851 bp (GRCm38)
  • A to G, chromosome 5 at 87,930,013 bp (GRCm38)
  • T to C, chromosome 6 at 58,215,882 bp (GRCm38)
  • T to A, chromosome 6 at 83,193,709 bp (GRCm38)
  • A to G, chromosome 7 at 5,480,748 bp (GRCm38)
  • A to G, chromosome 7 at 6,305,322 bp (GRCm38)
  • C to A, chromosome 7 at 50,062,639 bp (GRCm38)
  • C to T, chromosome 7 at 74,297,248 bp (GRCm38)
  • A to T, chromosome 7 at 108,508,948 bp (GRCm38)
  • A to T, chromosome 8 at 104,340,726 bp (GRCm38)
  • G to T, chromosome 8 at 104,340,727 bp (GRCm38)
  • C to T, chromosome 8 at 108,704,218 bp (GRCm38)
  • T to A, chromosome 8 at 113,775,278 bp (GRCm38)
  • T to C, chromosome 9 at 18,641,806 bp (GRCm38)
  • T to A, chromosome 9 at 19,584,672 bp (GRCm38)
  • T to A, chromosome 9 at 22,002,332 bp (GRCm38)
  • T to C, chromosome 9 at 37,418,493 bp (GRCm38)
  • C to T, chromosome 9 at 86,543,040 bp (GRCm38)
  • A to T, chromosome 9 at 86,561,885 bp (GRCm38)
  • G to A, chromosome 9 at 121,642,927 bp (GRCm38)
  • C to T, chromosome 10 at 14,426,996 bp (GRCm38)
  • T to C, chromosome 10 at 71,376,720 bp (GRCm38)
  • C to T, chromosome 10 at 81,080,167 bp (GRCm38)
  • C to T, chromosome 11 at 72,037,427 bp (GRCm38)
  • A to G, chromosome 11 at 97,363,147 bp (GRCm38)
  • C to A, chromosome 11 at 101,302,006 bp (GRCm38)
  • T to A, chromosome 11 at 106,766,979 bp (GRCm38)
  • T to C, chromosome 11 at 120,873,618 bp (GRCm38)
  • A to G, chromosome 13 at 13,975,551 bp (GRCm38)
  • A to G, chromosome 13 at 19,675,748 bp (GRCm38)
  • A to G, chromosome 13 at 23,746,957 bp (GRCm38)
  • A to G, chromosome 13 at 42,159,737 bp (GRCm38)
  • A to T, chromosome 13 at 76,130,328 bp (GRCm38)
  • T to A, chromosome 14 at 56,158,355 bp (GRCm38)
  • T to C, chromosome 15 at 58,052,675 bp (GRCm38)
  • A to T, chromosome 15 at 100,800,588 bp (GRCm38)
  • G to A, chromosome 16 at 4,849,289 bp (GRCm38)
  • T to A, chromosome 17 at 18,929,071 bp (GRCm38)
  • T to A, chromosome 17 at 20,993,807 bp (GRCm38)
  • T to C, chromosome 17 at 28,638,586 bp (GRCm38)
  • C to T, chromosome 18 at 44,405,294 bp (GRCm38)
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9422 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069226-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.