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Strain Name:
C57BL/6J-MtgxR9683Btlr/Mmmh
Stock Number:
069476-MU
Citation ID:
RRID:MMRRC_069476-MU
Other Names:
R9683 (G1)
Major Collection:

Strain Information

Npl
Name: N-acetylneuraminate pyruvate lyase
Synonyms: 0610033B02Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 74091
Homologene: 12533
Ubr5
Name: ubiquitin protein ligase E3 component n-recognin 5
Synonyms: 4432411E13Rik, Edd1, Edd
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 70790
VEGA: 15
Homologene: 9295
Bicral
Name: BRD4 interacting chromatin remodeling complex associated protein like
Synonyms: BC032203, Gltscr1l
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 210982
VEGA: 17
Homologene: 19366
Cnot6
Name: CCR4-NOT transcription complex, subunit 6
Synonyms: A230103N10Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 104625
Homologene: 69187
Anln
Name: anillin, actin binding protein
Synonyms: 1110037A17Rik, Scraps, 2900037I21Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 68743
VEGA: 9
Homologene: 41281
Ankrd11
Name: ankyrin repeat domain 11
Synonyms: 2410104C19Rik, 9530048I21Rik, 3010027A04Rik, Yod
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 77087
Homologene: 69134
Ppp1r12a
Name: protein phosphatase 1, regulatory subunit 12A
Synonyms: 1200015F06Rik, Mypt1, D10Ertd625e, 5730577I22Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 17931
VEGA: 10
HGNC: HGNC:7618
Homologene: 1855
Genetic Alterations
ENU-induced transitions at the following base pair locations:
  • T to C, chromosome 1 at 58,239,303 bp (GRCm38)
  • G to C, chromosome 1 at 119,527,018 bp (GRCm38)
  • A to G, chromosome 1 at 143,746,554 bp (GRCm38)
  • A to C, chromosome 1 at 153,545,284 bp (GRCm38)
  • A to G, chromosome 1 at 158,664,049 bp (GRCm38)
  • A to G, chromosome 1 at 165,222,578 bp (GRCm38)
  • A to G, chromosome 1 at 181,598,944 bp (GRCm38)
  • A to G, chromosome 2 at 73,362,436 bp (GRCm38)
  • T to A, chromosome 2 at 131,079,316 bp (GRCm38)
  • A to T, chromosome 2 at 162,970,308 bp (GRCm38)
  • C to T, chromosome 2 at 172,426,736 bp (GRCm38)
  • G to A, chromosome 2 at 180,606,529 bp (GRCm38)
  • A to G, chromosome 3 at 38,889,183 bp (GRCm38)
  • A to T, chromosome 3 at 51,542,690 bp (GRCm38)
  • T to A, chromosome 3 at 67,349,995 bp (GRCm38)
  • A to T, chromosome 3 at 95,294,865 bp (GRCm38)
  • C to T, chromosome 3 at 124,406,446 bp (GRCm38)
  • T to C, chromosome 4 at 3,871,186 bp (GRCm38)
  • T to A, chromosome 4 at 46,025,946 bp (GRCm38)
  • A to T, chromosome 4 at 154,892,828 bp (GRCm38)
  • T to A, chromosome 5 at 109,363,811 bp (GRCm38)
  • T to C, chromosome 5 at 110,836,015 bp (GRCm38)
  • T to C, chromosome 5 at 137,464,514 bp (GRCm38)
  • A to G, chromosome 6 at 137,056,254 bp (GRCm38)
  • C to T, chromosome 6 at 142,359,920 bp (GRCm38)
  • T to A, chromosome 7 at 18,682,583 bp (GRCm38)
  • T to A, chromosome 7 at 45,356,575 bp (GRCm38)
  • A to G, chromosome 7 at 103,469,950 bp (GRCm38)
  • A to G, chromosome 7 at 105,748,897 bp (GRCm38)
  • T to C, chromosome 8 at 105,376,291 bp (GRCm38)
  • C to T, chromosome 8 at 122,890,943 bp (GRCm38)
  • T to C, chromosome 9 at 22,278,503 bp (GRCm38)
  • A to T, chromosome 9 at 22,372,240 bp (GRCm38)
  • T to G, chromosome 9 at 94,670,182 bp (GRCm38)
  • T to A, chromosome 9 at 108,827,323 bp (GRCm38)
  • AAGA to AAGAGA, chromosome 10 at 21,992,391 bp (GRCm38)
  • T to A, chromosome 10 at 108,260,886 bp (GRCm38)
  • T to C, chromosome 11 at 5,444,465 bp (GRCm38)
  • A to T, chromosome 11 at 49,689,337 bp (GRCm38)
  • T to A, chromosome 11 at 67,931,813 bp (GRCm38)
  • G to A, chromosome 11 at 70,982,923 bp (GRCm38)
  • G to A, chromosome 11 at 73,374,985 bp (GRCm38)
  • A to C, chromosome 11 at 94,097,742 bp (GRCm38)
  • G to A, chromosome 11 at 98,996,857 bp (GRCm38)
  • A to G, chromosome 11 at 120,495,486 bp (GRCm38)
  • G to T, chromosome 12 at 113,490,556 bp (GRCm38)
  • T to C, chromosome 13 at 12,434,259 bp (GRCm38)
  • C to A, chromosome 13 at 50,474,981 bp (GRCm38)
  • T to A, chromosome 14 at 61,213,432 bp (GRCm38)
  • A to G, chromosome 14 at 64,031,677 bp (GRCm38)
  • A to T, chromosome 14 at 103,688,185 bp (GRCm38)
  • C to T, chromosome 15 at 37,978,027 bp (GRCm38)
  • T to A, chromosome 15 at 89,573,237 bp (GRCm38)
  • T to A, chromosome 16 at 33,919,965 bp (GRCm38)
  • A to G, chromosome 17 at 33,242,613 bp (GRCm38)
  • T to C, chromosome 17 at 46,812,018 bp (GRCm38)
  • C to T, chromosome 18 at 58,053,027 bp (GRCm38)
  • T to A, chromosome 19 at 3,815,587 bp (GRCm38)
  • T to C, chromosome 19 at 9,007,355 bp (GRCm38)
  • T to C, chromosome 19 at 11,511,733 bp (GRCm38)
  • T to A, chromosome 19 at 45,010,183 bp (GRCm38)
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9683 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069476-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.