This allele harbors a deletion of most of the 1,929-nucleotide coding region of Fzd1. The deletion removed 614 of the 643 amino acids in the coding region.

NA

Fzd1 homozygous knockout mice exhibit changes in sleep architecture, including an increase in daytime sleep and increased sleep bout length during the light/rest phase. The mice also exhibit a decrease in night sleep and a decrease in the number of nightly naps. Further, Fzd1 homozygous knockout mice exhibit increased edge time and immobility in open-field conditions.

Fzd1 knockout mice was generated using the CRISPR/Cas9 method. Two single guide RNAs (sgRNAs), one target near the N-terminus and the other target near the C-terminus of the Fzd1 coding region, were designed and purchased from Synthego. The two sgRNAs were co-microinjected with Cas9 mRNA into the zygotes collected from C57BL/6N mice. 2-cell stage embryos were implanted into the oviducts of pseudo-pregnant surrogate mothers (CD1). Offspring born to the foster mothers were genotyped by PCR and sequencing. Founders with the confirmed deletion between the two sgRNA cutting sites were bred with wild-type C57BL/6N mice and propagated for more than five generations to generate knockout mouse lines.

C57BL/6N

The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.

Susan Harbison, Ph.D., National Institutes of Health, National Heart, Lung, and Blood Institute (NHLB).