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Strain Detail Sheet

Strain Name:
C57BL/6N-Fzd1em1Lsg/Mmjax
Stock Number:
071899-JAX
Citation ID:
RRID:MMRRC_071899-JAX
Other Names:
Fzd1 knockout (frizzled class receptor 1 knockout)

Strain Information

Gene Details

Fzd1
Name: frizzled class receptor 1
Synonyms: FZ-1, Fz1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 14362
HGNC: HGNC:4038
Homologene: 20750

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
This allele harbors a deletion of most of the 1,929-nucleotide coding region of Fzd1. The deletion removed 614 of the 643 amino acids in the coding region.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
    • ES Cell Line
    NA

    Strain Description

    Phenotype
    Fzd1 homozygous knockout mice exhibit changes in sleep architecture, including an increase in daytime sleep and increased sleep bout length during the light/rest phase. The mice also exhibit a decrease in night sleep and a decrease in the number of nightly naps. Further, Fzd1 homozygous knockout mice exhibit increased edge time and immobility in open-field conditions.
    Strain GQC Summary
    The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
    Suggested Control Mice
    Littermates of all relevant genotypes.

    Research Applications

    • Developmental Biology
    • Neurobiology

    Strain Origin

    Donor
    Susan Harbison, Ph.D., National Institutes of Health, National Heart, Lung, and Blood Institute (NHLB).
    Primary Reference

    Gessner NR, Peiravi M, Zhang F, Yimam S, Springer D, Harbison ST. A conserved role for frizzled in sleep architecture. Sleep Adv. 2023 Nov 28;4(1):zpad045. doi: 10.1093/sleepadvances/zpad045. (Medline PMID: 38033424)

    Strain Development
    Fzd1 knockout mice was generated using the CRISPR/Cas9 method. Two single guide RNAs (sgRNAs), one target near the N-terminus and the other target near the C-terminus of the Fzd1 coding region, were designed and purchased from Synthego. The two sgRNAs were co-microinjected with Cas9 mRNA into the zygotes collected from C57BL/6N mice. 2-cell stage embryos were implanted into the oviducts of pseudo-pregnant surrogate mothers (CD1). Offspring born to the foster mothers were genotyped by PCR and sequencing. Founders with the confirmed deletion between the two sgRNA cutting sites were bred with wild-type C57BL/6N mice and propagated for more than five generations to generate knockout mouse lines.


    Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

    Colony and Husbandry Information

    Special Considerations

    N/A

    Health Status Report

    For more information about this colony's health status contact csmmrrc@jax.org

    Appearance

    Coat Color
    Black
    Eye
    Black

    Breeding

    MMRRC Breeding System
    Random intra-strain mating
    Generation
    N/A
    Overall Breeding Performance
    Good

    Reproductive Statistics

    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Yes Yes
    Homozygotes are fertile: Yes Yes
    Heterozygotes are fertile: Yes Yes
    Age Reproductive Decline: Undetermined Undetermined
    Average litter size
    Less than 4
    Recommended wean age
    3 Weeks
    Average Pups Weaned
    Less than 4

    Order Request Information

    Availability Level

    Limited quantities of breeder mice (up to 2 males and 2 females or 4 mice) per investigator per month are available from a live colony, usually available to ship in under 12 weeks. Larger quantities may be available, please contact the distributing center directly at csmmrrc@jax.org for more details.

    Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

    A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Vincent Kolesnitchenko.

    Conditions of Distribution

    A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Vincent Kolesnitchenko

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    Fees

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*    		 Units Notes
    071899-JAX-HET-F
    071899-JAX-HET-M
    		Heterozygous / Hemizygous Female
    Heterozygous / Hemizygous Male
    		 $229.00 / $229.00
    Non-Profit / For-Profit    		 Per Mouse The csmmrrc@jax.org may assess additional fees for any special requests (e.g., specific age or weight of mice, etc.).
    071899-JAX-SPERM Cryo-preserved spermatozoa $459.00 / $459.00
    Non-Profit / For-Profit    		 Aliquot Approximate quantity3
    Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.