Strain Detail Sheet

Strain Name:
C57BL/6J-Cd8am1Btlr/Mmmh
Stock Number:
038205--TBD-
Citation ID:
RRID:MMRRC_038205--TBD-
Other Names:
alfalfa
Major Collection:
Beutler Mutagenetix

Strain Information

Gene Details

Cd8am1Btlr
Name: CD8 subunit alpha; mutation 1, Bruce Beutler
Synonyms: alfalfa
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
Cd8a
Name: CD8 subunit alpha
Synonyms: Ly-2, Ly-35, Lyt-2, Ly-B
Type: Gene
Species: Mouse
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 12525
HGNC: HGNC:1706
Homologene: 133777
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Genetic Alterations
ENU-induced T to A transversion at base pair 71,373,728 on chromosome 6 (GRCm38), corresponding to base pair 20,957 of the genomic DNA sequence NC_000072 encoding the Cd8a gene. The mutation is positioned in exon 2 out of 6 exons (variant 1) or 5 exons (variant 2). The mutation affects nucleotide 302 of Cd8a mRNA variants 1 (NM_001081110.2) and 2 (NM_009857.1). The mutation results in a valine (V) to aspartic acid (D) substitution at position 59 (V59D) of both variants of the CD8 antigen, ? chain (CD8?).

Strain Description

Phenotype

Homozygous: Mice had reduced frequencies of CD8+ T cells (~10% of C57BL/6J), as well as reduced frequencies of na ve CD8+ T cells among total CD8+ T cells, elevated frequencies of central and effector memory CD8+ T cells, and elevated mean fluorescence intensity of CD44 immunostaining on CD8+ T cells, all in the peripheral blood. In addition, increased frequencies of CD4+ T cells were observed among total cells or CD3+ T cells in the peripheral blood (~150% of C57BL/6J). Five days after mouse cytomegalovirus (MCMV) infection, several mice displayed elevated MCMV titers in the spleen, suggesting increased susceptibility to the virus.

Heterozygous: No abnormal phenotype detected.

Mammalian Phenotype Terms
Allelic Composition: (Genetic Background: )
  • immune system
    • abnormal CD4-positive T cell differentiation [MP:0008076]
    • abnormal CD8-positive, alpha-beta T cell differentiation [MP:0008080]
    • increased susceptibility to viral infection [MP:0002418]
  • hematopoietic system
    • abnormal CD4-positive T cell differentiation [MP:0008076]
    • abnormal CD8-positive, alpha-beta T cell differentiation [MP:0008080]
Strain Development
Original mutant was a C57BL/6J G3 ENU-induced mutant; all subsequent crosses to maintain strain were on C57BL/6J background only.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact . Older strains may not have this information.

Research Applications

  • Immunology and Inflammation
  • Virology

Strain Origin

Primary Reference

Zeng M, Moresco EMY, Beutler B. Record for alfalfa. MUTAGENETIX, B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Health Status Report

For more information about this colony's health status contact

Appearance

Coat Color
Black

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable:
Homozygotes are fertile:
Heterozygotes are fertile:
Age Reproductive Decline: Unknown Unknown

Order Request Information

Availability Level

The availability level for this product has not been determined.

Conditions of Distribution

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