Strain Detail Sheet

Strain Name:
C57BL/6J-Map3k8m3Btlr/Mmmh
Stock Number:
038217--TBD-
Citation ID:
RRID:MMRRC_038217--TBD-
Other Names:
gnostic_gospel
Major Collection:
Beutler Mutagenetix

Strain Information

Gene Details

Map3k8m3Btlr
Name: mitogen-activated protein kinase kinase kinase 8; mutation 3, Bruce Beutler
Synonyms: gnostic_gospel
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
Map3k8
Name: mitogen-activated protein kinase kinase kinase 8
Synonyms: Tpl2, Cot, Cot/Tpl2, Tpl-2, c-COT
Type: Gene
Species: Mouse
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 26410
HGNC: HGNC:6860
Homologene: 3812
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Genetic Alterations
ENU-induced C to T transition at base pair 4,333,965 (v38) on chromosome 18, or base pair 19,517 in the GenBank genomic region NC_000084 for the Map3k8 gene. The mutation corresponds to residue 1,250 in the mRNA sequence NM_008713 within exon 7 of 8 total exons. The mutation results in an arginine (R) to cysteine (C) substitution at position 376 (R376C) in the TPL2 protein.

Strain Description

Phenotype

Homozygous: Mice showed reduced TNFα secretion in response to the Toll-like receptor ligands CpG oligodeoxynucleotide (TLR9), lipopolysaccharide (TLR4), Pam3CSK4 (TLR1/2), and poly(I:C) (TLR3).

Heterozygous: Mice showed reduced TNFα secretion in response to the Toll-like receptor ligands CpG oligodeoxynucleotide (TLR9), lipopolysaccharide (TLR4), Pam3CSK4 (TLR1/2), and poly(I:C) (TLR3); phenotype is intermediate between wild-type and homozygous gnostic_gospel mice.

Mammalian Phenotype Terms
Allelic Composition: (Genetic Background: )
  • immune system
    • decreased macrophage cytokine production [MP:0011079]
    • decreased tumor necrosis factor secretion [MP:0008561]
  • hematopoietic system
    • decreased macrophage cytokine production [MP:0011079]
Strain Development
Original mutant was a C57BL/6J G3 ENU-induced mutant; all subsequent crosses to maintain strain were on C57BL/6J background only.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact . Older strains may not have this information.

Research Applications

  • Immunology and Inflammation

Strain Origin

Primary Reference

Sun L, Zhang Z, Shi H, Murray AR, Beutler B. Record for gnostic_gospel. MUTAGENETIX, B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Health Status Report

For more information about this colony's health status contact

Appearance

Coat Color
Black

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Unknown Unknown

Order Request Information

Availability Level

The availability level for this product has not been determined.

Conditions of Distribution

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

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