Strain Detail Sheet

Strain Name:
C57BL/6J-Map3k8m2Btlr/Mmmh
Stock Number:
038246--TBD-
Citation ID:
RRID:MMRRC_038246--TBD-
Other Names:
juicy
Major Collection:
Beutler Mutagenetix

Strain Information

Gene Details

Map3k8m2Btlr
Name: mitogen-activated protein kinase kinase kinase 8; mutation 2, Bruce Beutler
Synonyms: juicy
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
Map3k8
Name: mitogen-activated protein kinase kinase kinase 8
Synonyms: Tpl2, Cot, Cot/Tpl2, Tpl-2, c-COT
Type: Gene
Species: Mouse
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 26410
HGNC: HGNC:6860
Homologene: 3812
Genome Browser [Click image to go to Jbrowse]



Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
ENU-induced T to G transversion at base pair 4,339,552 (v38) on chromosome 18, or base pair 13,930 in the GenBank genomic region NC_000084 encoding the Map3k8 gene. The mutation corresponds to residue 942 in the NM_007746 mRNA sequence in exon 5 of 8 total exons. The mutation results in a leucine (L) to arginine (R) substitution at amino acid residue 273 in the TPL2 protein.

Strain Description

Phenotype

Homozygous: Mice showed diminished LPS-induced TNFα secretion (i.e., defective TLR4 signaling).

Heterozygous: No abnormal phenotype detected.

Mammalian Phenotype Terms
Allelic Composition: (Genetic Background: )
  • immune system
Strain Development
Original mutant was a C57BL/6J G3 ENU-induced mutant; all subsequent crosses to maintain strain were on C57BL/6J background only.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact . Older strains may not have this information.

Research Applications

  • Immunology and Inflammation

Strain Origin

Primary Reference

Wang Y, Shi H, Murray AR, Beutler B. Record for juicy. MUTAGENETIX, B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Health Status Report

For more information about this colony's health status contact

Appearance

Coat Color
Black

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Unknown Unknown

Order Request Information

Availability Level

The availability level for this product has not been determined.

Conditions of Distribution

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

- Products for this strain are Not Yet Available for Ordering
- If you register interest in this strain, you will be notified when it becomes available for ordering.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.