Strain Name:
C57BL/6J-MtgxR2213Btlr/Mmmh
Stock Number:
040215-MU
Citation ID:
RRID:MMRRC_040215-MU
Other Names:
R2213 (G1), C57BL/6J-MtgxR2213Btlr
Major Collection:

Strain Information

Tyr
Name: tyrosinase
Synonyms: skc35, Oca1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 22173
Homologene: 30969
Uchl3
Name: ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase)
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 50933
VEGA: 14
Homologene: 4377
Wrn
Name: Werner syndrome RecQ like helicase
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 22427
Homologene: 6659
Ylpm1
Name: YLP motif containing 1
Synonyms: ZAP, Zap3, A930013E17Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 56531
Homologene: 87707
Rbm25
Name: RNA binding motif protein 25
Synonyms: 2610015J01Rik, A130095G20Rik, 2600011C06Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 67039
Flnb
Name: filamin, beta
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 286940
HGNC: HGNC:3755
Homologene: 37480
Smad2
Name: SMAD family member 2
Synonyms: Madr2, Smad 2, Madh2, 7120426M23Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 17126
HGNC: HGNC:6768
Homologene: 21197
Taf4
Name: TATA-box binding protein associated factor 4
Synonyms: Taf2c1, TAFII135, TAFII130, Taf4a
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228980
Homologene: 55723
Mtf2
Name: metal response element binding transcription factor 2
Synonyms: M96, 9230112N11Rik, C76717, Pcl2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 17765
Homologene: 7207
Hunk
Name: hormonally upregulated Neu-associated kinase
Synonyms: Mak-v, Bstk1
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 26559
VEGA: 16
Homologene: 8742
Ddb1
Name: damage specific DNA binding protein 1
Synonyms: p127-Ddb1, damage-specific DNA-binding protein, DNA repair, DNA repair protein
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 13194
VEGA: 19
HGNC: HGNC:2717
Homologene: 1448
Rif1
Name: replication timing regulatory factor 1
Synonyms: 5730435J01Rik, 6530403D07Rik, D2Ertd145e
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 51869
Homologene: 41231
Slit2
Name: slit guidance ligand 2
Synonyms: Slil3, Drad-1, E130320P19Rik, E030015M03Rik, b2b1200.1Clo
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 20563
Homologene: 3516
Cul7
Name: cullin 7
Synonyms: p193, 2510004L20Rik, p185, C230011P08Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 66515
Homologene: 56683
Syngap1
Name: synaptic Ras GTPase activating protein 1 homolog (rat)
Synonyms: Syngap
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 240057
Homologene: 84739
Strip2
Name: striatin interacting protein 2
Synonyms: Myoscape, D330017J20Rik, Fam40b
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 320609
Homologene: 66198
Crispld1
Name: cysteine-rich secretory protein LCCL domain containing 1
Synonyms: Cocoacrisp
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 83691
Homologene: 12857
Lama1
Name: laminin, alpha 1
Synonyms: Lama
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 16772
VEGA: 17
HGNC: HGNC:6481
Homologene: 21146
Eps15
Name: epidermal growth factor receptor pathway substrate 15
Synonyms: 2410112D09Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 13858
HGNC: HGNC:3419
Homologene: 128359
Npat
Name: nuclear protein in the AT region
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 244879
HGNC: HGNC:7896
Homologene: 1888
Mpdz
Name: multiple PDZ domain crumbs cell polarity complex component
Synonyms: MUPP1, B930003D11Rik, multiple PDZ domain protein
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 17475
HGNC: HGNC:7208
Homologene: 2841
Rps10
Name: ribosomal protein S10
Synonyms: 2210402A09Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 67097
Homologene: 788
Snrnp48
Name: small nuclear ribonucleoprotein 48 (U11/U12)
Synonyms: 1110050F08Rik, 6530403A03Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 67797
VEGA: 13
Homologene: 12191
Nup58
Name: nucleoporin 58
Synonyms: 1700017F11Rik, Nupl1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 71844
VEGA: 14
Homologene: 40924
Gle1
Name: GLE1 RNA export mediator
Synonyms: 4933405K21Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 74412
HGNC: HGNC:4315
Homologene: 20379
C87436
Name: expressed sequence C87436
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 232196
Homologene: 9897
Jag1
Name: jagged 1
Synonyms: Serrate-1, Headturner, Htu, ABE2, Ozz, Gsfabe2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 16449
HGNC: HGNC:6188
Homologene: 180
Lrrc4c
Name: leucine rich repeat containing 4C
Synonyms: 6430556C10Rik, netrin g1 ligand, NGL-1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 241568
Homologene: 18696
Lrp1
Name: low density lipoprotein receptor-related protein 1
Synonyms: CD91, A2mr, b2b1554Clo
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 16971
HGNC: HGNC:6692
Homologene: 1744
Dnah12
Name: dynein, axonemal, heavy chain 12
Synonyms: Hdhc3, HL-19, DLP12, HL19, DHC3, LOC380889, 4921531P07Rik, Dnahc7l, Dnahc12
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 110083
HGNC: HGNC:2943
Homologene: 56821
Nelfe
Name: negative elongation factor complex member E, Rdbp
Synonyms: NELF-E, Rdbp
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 27632
Homologene: 134736
Or4a78
Name: olfactory receptor family 4 subfamily A member 78
Synonyms: GA_x6K02T2Q125-51109312-51108356, MOR231-15P, MOR231-24_p, Olfr1251
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 259145
Homologene: 74065
Gcnt3
Name: glucosaminyl (N-acetyl) transferase 3, mucin type
Synonyms: 2010013H22Rik, 2210021I22Rik, 2210401J11Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 72077
VEGA: 9
HGNC: HGNC:4205
Homologene: 20993
Eaf2
Name: ELL associated factor 2
Synonyms: Traits, Festa, U19, FESTA-S, FESTA-L
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 106389
Homologene: 10208
Sis
Name: sucrase isomaltase
Synonyms: Si-s, sucrase-isomaltase, 2010204N08Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 69983
Homologene: 37424
Stat4
Name: signal transducer and activator of transcription 4
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 20849
Homologene: 20679
Lrrc43
Name: leucine rich repeat containing 43
Synonyms: LOC381741
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 381741
Homologene: 17662
Megf10
Name: multiple EGF-like-domains 10
Synonyms: LOC240312, 3000002B06Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 70417
Homologene: 23771
Dclk1
Name: doublecortin-like kinase 1
Synonyms: CPG16, DCLK, 1700113D08Rik, 2810480F11Rik, Dcl, Click-I, Dcamkl1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 13175
HGNC: HGNC:2700
Homologene: 130530
Csmd3
Name: CUB and Sushi multiple domains 3
Synonyms: 4930500N14Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 239420
Homologene: 65982
Flg
Name: filaggrin
Synonyms: profilaggrin, fillagrin, ft
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 14246
VEGA: 3
Synpo2l
Name: synaptopodin 2-like
Synonyms: 1110054M18Rik, Chap
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 68760
Homologene: 23499
Ttc21a
Name: tetratricopeptide repeat domain 21A
Synonyms: 4921538N17Rik, Thm2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 74052
Homologene: 14728
Fscb
Name: fibrous sheath CABYR binding protein
Synonyms: EG623046
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 623046
VEGA: 12
Homologene: 136275
Anxa1
Name: annexin A1
Synonyms: Lpc-1, Lpc1, Anx-1, Anx-A1, C430014K04Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 16952
HGNC: HGNC:533
Homologene: 563
Galntl5
Name: UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase-like 5
Synonyms: 1700021B12Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 67909
Homologene: 12210
Arhgef4
Name: Rho guanine nucleotide exchange factor 4
Synonyms: 9330140K16Rik, Asef, C230030N03Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 226970
HGNC: HGNC:684
Homologene: 49414
Gpr87
Name: G protein-coupled receptor 87
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 84111
HGNC: HGNC:4538
Homologene: 13021
Sox30
Name: SRY (sex determining region Y)-box 30
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 214105
Homologene: 34027
Cyp2j5
Name: cytochrome P450, family 2, subfamily j, polypeptide 5
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 13109
HGNC: HGNC:2634
Homologene: 116530
Vwa5b1
Name: von Willebrand factor A domain containing 5B1
Synonyms: 4931403E03Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 75718
Homologene: 19431
Mak
Name: male germ cell-associated kinase
Synonyms: A930010O05Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 17152
VEGA: 13
HGNC: HGNC:6816
Homologene: 31344
Nin
Name: ninein
Synonyms: 3110068G20Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 18080
Homologene: 40632
Fbxl2
Name: F-box and leucine-rich repeat protein 2
Synonyms: 2810423A21Rik, Fbl3
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 72179
Homologene: 135814
Dixdc1
Name: DIX domain containing 1
Synonyms: 4930563F16Rik, Ccd1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 330938
Homologene: 82369
Gopc
Name: golgi associated PDZ and coiled-coil motif containing
Synonyms: GOPC1, 2210402P09Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 94221
VEGA: 10
Homologene: 10695
Itih4
Name: inter alpha-trypsin inhibitor, heavy chain 4
Synonyms: Itih-4
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 16427
HGNC: HGNC:6169
Homologene: 1670
Klk1b1
Name: kallikrein 1-related peptidase b1
Synonyms: mGK-1, TK, tissue kallikrein, mK1, Klk1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 16623
Homologene: 68141
Gstm2
Name: glutathione S-transferase, mu 2
Synonyms: Gstb-2, Gstb2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 14863
Homologene: 121492
Rmnd1
Name: required for meiotic nuclear division 1 homolog
Synonyms: 0610042C05Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 66084
Homologene: 5675
Haus6
Name: HAUS augmin-like complex, subunit 6
Synonyms: D4Ertd27e, 6230416J20Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230376
Homologene: 9760
Cd163
Name: CD163 antigen
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 93671
HGNC: HGNC:1631
Homologene: 128811
Ltk
Name: leukocyte tyrosine kinase
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 17005
HGNC: HGNC:6721
Homologene: 130502
Ces1a
Name: carboxylesterase 1A
Synonyms: Gm4976
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 244595
HGNC: HGNC:1863
Homologene: 134142
H1f7
Name: H1.7 linker histone
Synonyms: 1700026P10Rik, H1T2, H1fnt, H1-7
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 70069
VEGA: 15
Pm20d2
Name: peptidase M20 domain containing 2
Synonyms: LOC242377, Acy1l2
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 242377
Homologene: 19025
Txndc8
Name: thioredoxin domain containing 8
Synonyms: 4930429J24Rik, Sptrx3, Sptrx-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 67402
Homologene: 41663
A530095I07Rik
Name: RIKEN cDNA A530095I07 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 210373
Prl7a2
Name: prolactin family 7, subfamily a, member 2
Synonyms: PLP-F, Prlpf
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 19114
Homologene: 49263
Rbms3
Name: RNA binding motif, single stranded interacting protein
Synonyms: 6720477E09Rik, 8430436O14Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 207181
Homologene: 49388
Cpm
Name: carboxypeptidase M
Synonyms: 5730456K23Rik, 1110060I01Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 70574
HGNC: HGNC:2311
Homologene: 35367
Pnlip
Name: pancreatic lipase
Synonyms: pancreatic triglyceride lipase, PTL, 1810007A24Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 69060
VEGA: 19
HGNC: HGNC:9155
Homologene: 30999
Akr1c12
Name: aldo-keto reductase family 1, member C12
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 622402
HGNC: HGNC:387
Homologene: 121208
AC239677.1
Name:
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Mecr
Name: mitochondrial trans-2-enoyl-CoA reductase
Synonyms: Nrbf1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 26922
Homologene: 5362
Fabp9
Name: fatty acid binding protein 9, testis
Synonyms: Tlbp, 1700007P10Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 21884
HGNC: HGNC:3563
Homologene: 40776
Slc35e4
Name: solute carrier family 35, member E4
Synonyms: A330108F03Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 103710
Homologene: 37409
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to C, chromosome 1 at 17,746,857 bp
  • G to A, chromosome 1 at 34,807,149 bp
  • A to T, chromosome 1 at 52,013,855 bp
  • T to C, chromosome 2 at 29,949,301 bp
  • GCCACCA to GCCA, chromosome 2 at 52,110,324 bp
  • A to T, chromosome 2 at 89,667,547 bp
  • G to A, chromosome 2 at 97,630,471 bp
  • T to C, chromosome 2 at 119,758,753 bp
  • G to T, chromosome 2 at 137,089,892 bp
  • A to T, chromosome 2 at 179,935,890 bp
  • A to G, chromosome 3 at 10,194,800 bp
  • G to A, chromosome 3 at 55,480,433 bp
  • A to T, chromosome 3 at 59,179,044 bp
  • C to T, chromosome 3 at 72,947,407 bp
  • T to C, chromosome 3 at 93,293,028 bp
  • C to T, chromosome 3 at 107,986,093 bp
  • A to T, chromosome 4 at 33,183,227 bp
  • T to A, chromosome 4 at 57,984,199 bp
  • A to G, chromosome 4 at 81,310,172 bp
  • C to T, chromosome 4 at 86,581,992 bp
  • T to C, chromosome 4 at 96,659,615 bp
  • A to G, chromosome 4 at 109,361,220 bp
  • T to A, chromosome 4 at 131,853,815 bp
  • T to C, chromosome 4 at 138,604,812 bp
  • A to G, chromosome 5 at 25,217,529 bp
  • G to T, chromosome 5 at 25,953,177 bp
  • A to G, chromosome 5 at 48,219,658 bp
  • A to G, chromosome 5 at 108,100,914 bp
  • G to A, chromosome 5 at 123,503,577 bp
  • A to G, chromosome 6 at 29,931,148 bp
  • G to A, chromosome 6 at 86,445,473 bp
  • A to G, chromosome 6 at 124,311,711 bp
  • T to C, chromosome 7 at 43,970,481 bp
  • T to G, chromosome 7 at 87,492,878 bp
  • T to C, chromosome 8 at 33,257,015 bp
  • G to T, chromosome 8 at 93,025,225 bp
  • A to G, chromosome 9 at 50,701,945 bp
  • A to G, chromosome 9 at 53,552,381 bp
  • A to T, chromosome 9 at 70,034,707 bp
  • A to G, chromosome 9 at 113,984,589 bp
  • A to T, chromosome 9 at 116,959,466 bp
  • A to G, chromosome 9 at 119,940,461 bp
  • A to G, chromosome 10 at 4,411,740 bp
  • A to T, chromosome 10 at 52,346,241 bp
  • T to C, chromosome 10 at 117,659,839 bp
  • T to A, chromosome 10 at 127,540,702 bp
  • T to C, chromosome 11 at 3,913,159 bp
  • C to T, chromosome 11 at 45,984,852 bp
  • A to T, chromosome 12 at 64,474,116 bp
  • A to G, chromosome 12 at 70,045,354 bp
  • A to T, chromosome 12 at 83,676,082 bp
  • A to G, chromosome 12 at 85,069,718 bp
  • T to C, chromosome 13 at 4,276,248 bp
  • T to A, chromosome 13 at 27,665,068 bp
  • T to A, chromosome 13 at 38,209,997 bp
  • C to T, chromosome 13 at 41,055,689 bp
  • T to C, chromosome 13 at 69,543,408 bp
  • AAGGAG to AAG, chromosome 14 at 7,881,652 bp
  • A to G, chromosome 14 at 20,660,666 bp
  • G to T, chromosome 14 at 26,739,330 bp
  • T to A, chromosome 14 at 30,890,713 bp
  • T to C, chromosome 14 at 60,239,496 bp
  • T to A, chromosome 14 at 101,666,670 bp
  • G to A, chromosome 15 at 47,820,447 bp
  • T to A, chromosome 15 at 98,256,338 bp
  • A to C, chromosome 16 at 36,808,008 bp
  • A to G, chromosome 16 at 90,432,617 bp
  • C to A, chromosome 17 at 26,953,069 bp
  • C to A, chromosome 17 at 27,630,499 bp
  • T to A, chromosome 17 at 34,853,883 bp
  • T to C, chromosome 17 at 46,651,472 bp
  • G to T, chromosome 17 at 67,777,034 bp
  • T to A, chromosome 18 at 57,288,009 bp
  • A to T, chromosome 18 at 76,304,626 bp
  • T to C, chromosome 19 at 10,608,327 bp
  • C to T, chromosome 19 at 20,382,875 bp
  • T to C, chromosome 19 at 58,673,770 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2213 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040215-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.