Strain Name:
C57BL/6J-MtgxR2993Btlr/Mmmh
Stock Number:
040528-MU
Citation ID:
RRID:MMRRC_040528-MU
Other Names:
R2993 (G1), C57BL/6J-MtgxR2993Btlr
Major Collection:

Strain Information

Gtf2h3
Name: general transcription factor IIH, polypeptide 3
Synonyms: 34kDa, 5033417D07Rik, D5Ertd679e, BTF2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 209357
HGNC: HGNC:4657
Homologene: 1160
Klhdc4
Name: kelch domain containing 4
Synonyms: G430025P05Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 234825
Homologene: 69234
Ago1
Name: argonaute RISC catalytic subunit 1
Synonyms: argonaute 1, Eif2c1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 236511
HGNC: HGNC:3262
Homologene: 81826
Aspm
Name: abnormal spindle microtubule assembly
Synonyms: Sha1, MCPH5, D330028K02Rik, Aspm, Calmbp1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12316
Homologene: 7650
Afdn
Name: afadin, adherens junction formation factor
Synonyms: AF6, S-afadin, 5033403D15Rik, Afadin, I-afadin, Mllt4
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 17356
HGNC: HGNC:7137
Homologene: 21202
Secisbp2l
Name: SECIS binding protein 2-like
Synonyms: 3110001I20Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 70354
Homologene: 18923
Ddx47
Name: DEAD box helicase 47
Synonyms: 4930588A18Rik, DEAD (Asp-Glu-Ala-Asp) box polypeptide 47
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 67755
Homologene: 6092
Lrrfip1
Name: leucine rich repeat (in FLII) interacting protein 1
Synonyms: Fliiap1, FLAP (FLI LRR associated protein)
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 16978
HGNC: HGNC:6702
Homologene: 48301
Dnah7a
Name: dynein, axonemal, heavy chain 7A
Synonyms: Dnahc7, LOC381341, Dnahc7a
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 627872
Homologene: 41287
Katnbl1
Name: katanin p80 subunit B like 1
Synonyms: 2410042D21Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 72425
Homologene: 11495
Eif3d
Name: eukaryotic translation initiation factor 3, subunit D
Synonyms: mouse translation initiation factor eIF3 p66, eIF3p66, 66/67kDa, Eif3s7
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 55944
VEGA: 15
HGNC: HGNC:3278
Homologene: 2782
Cdc7
Name: cell division cycle 7
Synonyms: Cdc7, Cdc7l1, muCdc7
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 12545
HGNC: HGNC:1745
Homologene: 31166
Mtmr4
Name: myotubularin related protein 4
Synonyms: FYVE zinc finger phosphatase, ESTM44, ZFYVE11, FYVE-DSP2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 170749
HGNC: HGNC:7452
Homologene: 3440
Abca16
Name: ATP-binding cassette, sub-family A member 16
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233810
Homologene: 132942
Atp4a
Name: ATPase, H+/K+ exchanging, gastric, alpha polypeptide
Synonyms: H+K+-transporting alpha 1, H+/K+-ATPase alpha
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 11944
HGNC: HGNC:819
Homologene: 68081
Rap1gap2
Name: RAP1 GTPase activating protein 2
Synonyms: LOC380710, Garnl4
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 380711
Homologene: 56695
Lrp1
Name: low density lipoprotein receptor-related protein 1
Synonyms: b2b1554Clo, A2mr, CD91
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 16971
HGNC: HGNC:6692
Homologene: 1744
Mrc1
Name: mannose receptor, C type 1
Synonyms: MR, CD206
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 17533
HGNC: HGNC:7228
Homologene: 37622
Eogt
Name: EGF domain specific O-linked N-acetylglucosamine transferase
Synonyms: A130022J15Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 101351
Homologene: 65276
Spata31e2
Name: spermatogenesis associated 31 subfamily E member 2
Synonyms: 4931408C20Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 210940
Homologene: 86827
Klrb1-ps1
Name: killer cell lectin-like receptor subfamily B member 1, pseudogene 1
Synonyms: Nkrp1e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 724020
Gabrb2
Name: gamma-aminobutyric acid type A receptor subunit beta 2
Synonyms: C030021G16Rik, Gabrb-2, C030002O17Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 14401
HGNC: HGNC:4082
Homologene: 7327
Vwa8
Name: von Willebrand factor A domain containing 8
Synonyms: 1300010F03Rik, 4932416F07Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 219189
VEGA: 14
Homologene: 44881
Vmn2r55
Name: vomeronasal 2, receptor 55
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 100042499
Homologene: 104040
Fbxl2
Name: F-box and leucine-rich repeat protein 2
Synonyms: Fbl3, 2810423A21Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 72179
Homologene: 135814
Epb42
Name: erythrocyte membrane protein band 4.2
Synonyms: Epb4.2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 13828
HGNC: HGNC:3381
Homologene: 93
Adarb2
Name: adenosine deaminase, RNA-specific, B2
Synonyms: RED2, Adar3
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 94191
HGNC: HGNC:227
Homologene: 10276
Cdh15
Name: cadherin 15
Synonyms: M cadherin, Mcad, Cdh14
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 12555
HGNC: HGNC:1754
Homologene: 3622
Zer1
Name: zyg-11 related, cell cycle regulator
Synonyms: Zyg11bl, C230075L19Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227693
Homologene: 4621
Cyp4a31
Name: cytochrome P450, family 4, subfamily a, polypeptide 31
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 666168
HGNC: HGNC:2642
Homologene: 128044
Mmp21
Name: matrix metallopeptidase 21
Synonyms: b2b2458Clo, b2b873Clo
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 214766
Homologene: 17519
1700003H04Rik
Name: RIKEN cDNA 1700003H04 gene
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 384775
Vmn1r224
Name: vomeronasal 1 receptor 224
Synonyms: Gm7673
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 665525
Homologene: 85978
Mrpl16
Name: mitochondrial ribosomal protein L16
Synonyms: 2310039D06Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 94063
VEGA: 19
Homologene: 9872
Gzmk
Name: granzyme K
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 14945
HGNC: HGNC:4711
Homologene: 20485
CT009495.1
Name:
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 26,685,828 bp
  • G to A, chromosome 1 at 53,503,554 bp
  • C to A, chromosome 1 at 91,105,234 bp
  • T to A, chromosome 1 at 139,474,236 bp
  • T to A, chromosome 2 at 14,319,087 bp
  • A to G, chromosome 2 at 30,101,897 bp
  • G to T, chromosome 2 at 112,408,618 bp
  • G to A, chromosome 2 at 121,029,044 bp
  • C to T, chromosome 2 at 125,740,737 bp
  • A to G, chromosome 3 at 124,578,535 bp
  • G to A, chromosome 4 at 115,569,820 bp
  • G to T, chromosome 4 at 126,440,046 bp
  • G to A, chromosome 5 at 106,973,898 bp
  • T to C, chromosome 5 at 124,583,934 bp
  • A to T, chromosome 6 at 97,118,954 bp
  • A to T, chromosome 6 at 129,121,029 bp
  • G to T, chromosome 6 at 135,018,981 bp
  • C to T, chromosome 7 at 12,684,955 bp
  • G to A, chromosome 7 at 30,720,225 bp
  • T to C, chromosome 7 at 120,535,161 bp
  • C to T, chromosome 7 at 133,678,986 bp
  • G to C, chromosome 8 at 121,806,581 bp
  • G to A, chromosome 8 at 122,862,024 bp
  • T to C, chromosome 9 at 113,989,416 bp
  • T to C, chromosome 10 at 127,610,381 bp
  • T to C, chromosome 11 at 42,597,649 bp
  • C to A, chromosome 11 at 74,407,322 bp
  • T to A, chromosome 11 at 87,604,997 bp
  • A to C, chromosome 13 at 8,713,716 bp
  • A to G, chromosome 13 at 113,180,477 bp
  • T to A, chromosome 14 at 37,107,445 bp
  • C to T, chromosome 14 at 79,058,661 bp
  • A to G, chromosome 15 at 77,961,705 bp
  • T to C, chromosome 17 at 13,891,000 bp
  • T to A, chromosome 17 at 20,419,210 bp
  • A to G, chromosome 19 at 11,774,531 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2993 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040528-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.