Strain Name:
C57BL/6J-MtgxR3435Btlr/Mmmh
Stock Number:
040653-MU
Citation ID:
RRID:MMRRC_040653-MU
Other Names:
R3435 (G1), C57BL/6J-MtgxR3435Btlr
Major Collection:

Strain Information

Notch3
Name: notch 3
Synonyms: hpbk, N3
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 18131
HGNC: HGNC:7883
Homologene: 376
Sema6d
Name: sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D
Synonyms: 1110067B02Rik, Sema6D-6, Sema6D-5, Sema6D-4, Sema6D-2, Sema6D-1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 214968
Homologene: 16195
Strn4
Name: striatin, calmodulin binding protein 4
Synonyms: ZIN, zinedin
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 97387
Homologene: 8378
Mast2
Name: microtubule associated serine/threonine kinase 2
Synonyms: MAST205, Mtssk
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 17776
Homologene: 7428
Mast4
Name: microtubule associated serine/threonine kinase family member 4
Synonyms: 4930420O11Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 328329
Homologene: 42094
Mdn1
Name: midasin AAA ATPase 1
Synonyms: LOC213784, 4833432B22Rik, D4Abb1e
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 100019
Homologene: 39689
Ift74
Name: intraflagellar transport 74
Synonyms: 1700029H06Rik, Cmg1, Ccdc2, b2b796Clo
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 67694
Homologene: 11831
Iqgap3
Name: IQ motif containing GTPase activating protein 3
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 404710
Homologene: 26400
Neurl1a
Name: neuralized E3 ubiquitin protein ligase 1A
Synonyms: Nlz, 2410129E16Rik, Neu1, Rnf67, Neur1, Neurl
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 18011
VEGA: 19
HGNC: HGNC:7761
Homologene: 32503
Atp7a
Name: ATPase, Cu++ transporting, alpha polypeptide
Synonyms: Menkes protein, MNK, br
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 11977
HGNC: HGNC:869
Homologene: 35
Dnah1
Name: dynein, axonemal, heavy chain 1
Synonyms: MDHC7, E030034C22Rik, B230373P09Rik, Dnahc1, G1-415-19, ferf1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 110084
VEGA: 14
HGNC: HGNC:2940
Homologene: 67131
P2rx4
Name: purinergic receptor P2X, ligand-gated ion channel 4
Synonyms: P2X4, D5Ertd444e
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 18438
HGNC: HGNC:8535
Homologene: 1923
Ttn
Name: titin
Synonyms: connectin, L56, 1100001C23Rik, mdm, D830007G01Rik, 2310036G12Rik, 2310074I15Rik, 2310057K23Rik, D330041I19Rik, shru
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 22138
Homologene: 130650
Pld1
Name: phospholipase D1
Synonyms: Pld1b, Pld1a
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 18805
HGNC: HGNC:9067
Homologene: 116234
AC159748.1
Name:
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Abca9
Name: ATP-binding cassette, sub-family A member 9
Synonyms: D630040K07Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 217262
HGNC: HGNC:39
Homologene: 33332
Mup6
Name: major urinary protein 6
Synonyms: Gm12544
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 620807
Homologene: 74304
4933409G03Rik
Name: RIKEN cDNA 4933409G03 gene
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227998
Osbpl3
Name: oxysterol binding protein-like 3
Synonyms: OSBP3, ORP3, 1200014M06Rik, 6720421I08Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 71720
Homologene: 49422
Tmx3
Name: thioredoxin-related transmembrane protein 3
Synonyms: 6430411B10Rik, A730024F05Rik, Txndc10
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 67988
VEGA: 18
Homologene: 10381
Prob1
Name: proline rich basic protein 1
Synonyms: LOC381148, Gm1614
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 381148
Homologene: 83773
Nod2
Name: nucleotide-binding oligomerization domain containing 2
Synonyms: F830032C23Rik, Card15, Nlrc2
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 257632
HGNC: HGNC:5331
Homologene: 11156
Larp1b
Name: La ribonucleoprotein 1B
Synonyms: 1700108L22Rik, 4933421B21Rik, Larp2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 214048
Homologene: 103869
Vmn1r209
Name: vomeronasal 1 receptor 209
Synonyms: Gm11315
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 432736
Homologene: 110880
Tmem117
Name: transmembrane protein 117
Synonyms: B930062P21Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 320709
VEGA: 15
Homologene: 12992
Stard13
Name: StAR related lipid transfer domain containing 13
Synonyms: GT650, DLC2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 243362
Homologene: 64844
Or1j1
Name: olfactory receptor family 1 subfamily J member 1
Synonyms: Y71, MOR136-14, GA_x6K02T2NLDC-33507606-33506665, Olfr3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 18328
HGNC: HGNC:8208
Homologene: 66579
Pbxip1
Name: pre B cell leukemia transcription factor interacting protein 1
Synonyms: 4732463H20Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 229534
Homologene: 10740
Fam47e
Name: family with sequence similarity 47, member E
Synonyms: LOC384198, Gm1381
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 384198
Homologene: 129958
Ppp2r2b
Name: protein phosphatase 2, regulatory subunit B, beta
Synonyms: 6330404L05Rik, PP2A-PR55B, PR55-BETA, SCA12, 2900026H06Rik, E130009M08Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 72930
HGNC: HGNC:9305
Homologene: 55833
AC132379.1
Name:
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Mafk
Name: v-maf musculoaponeurotic fibrosarcoma oncogene family, protein K (avian)
Synonyms: Nfe2u, p18 NF-E2, NF-E2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 17135
HGNC: HGNC:6782
Homologene: 1770
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to A, chromosome 1 at 116,821,623 bp
  • T to A, chromosome 2 at 36,812,678 bp
  • A to C, chromosome 2 at 68,601,845 bp
  • T to C, chromosome 2 at 76,878,718 bp
  • T to C, chromosome 2 at 124,660,274 bp
  • A to T, chromosome 3 at 28,124,623 bp
  • T to C, chromosome 3 at 41,034,098 bp
  • A to G, chromosome 3 at 88,094,604 bp
  • T to C, chromosome 3 at 89,447,236 bp
  • T to C, chromosome 4 at 32,733,726 bp
  • G to T, chromosome 4 at 60,004,116 bp
  • A to G, chromosome 4 at 94,621,852 bp
  • A to T, chromosome 4 at 116,308,095 bp
  • T to A, chromosome 5 at 92,585,362 bp
  • T to A, chromosome 5 at 122,725,070 bp
  • C to T, chromosome 5 at 139,800,307 bp
  • A to T, chromosome 5 at 151,042,179 bp
  • A to T, chromosome 6 at 50,348,070 bp
  • T to C, chromosome 7 at 16,837,633 bp
  • A to G, chromosome 8 at 88,664,009 bp
  • T to C, chromosome 10 at 5,348,565 bp
  • T to C, chromosome 11 at 110,154,430 bp
  • A to T, chromosome 13 at 22,806,097 bp
  • A to G, chromosome 13 at 102,787,379 bp
  • T to C, chromosome 14 at 31,316,674 bp
  • T to C, chromosome 15 at 95,094,692 bp
  • A to T, chromosome 17 at 32,158,618 bp
  • T to C, chromosome 18 at 35,654,241 bp
  • T to A, chromosome 18 at 42,741,109 bp
  • T to A, chromosome 18 at 90,527,904 bp
  • T to C, chromosome 19 at 47,257,525 bp
  • G to A, chromosome X at 106,094,857 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3435 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040653-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.