Strain Name:
C57BL/6J-MtgxR4452Btlr/Mmmh
Stock Number:
041713-MU
Citation ID:
RRID:MMRRC_041713-MU
Other Names:
R4452 (G1), C57BL/6J-MtgxR4452Btlr
Major Collection:

Strain Information

Scarb1
Name: scavenger receptor class B, member 1
Synonyms: Cla-1, D5Ertd460e, Hdlq1, Chohd1, Hlb398, SRBI, Srb1, Cd36l1, Chohd1, SR-BI
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 20778
HGNC: HGNC:1664
Homologene: 21132
Ptf1a
Name: pancreas specific transcription factor, 1a
Synonyms: bHLHa29, PTF1-p48
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 19213
Homologene: 10304
Pzp
Name: PZP, alpha-2-macroglobulin like
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 11287
Homologene: 104112
Xpnpep1
Name: X-prolyl aminopeptidase (aminopeptidase P) 1, soluble
Synonyms: D230045I08Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 170750
Homologene: 6424
Scn8a
Name: sodium channel, voltage-gated, type VIII, alpha
Synonyms: nmf2, nur14, C630029C19Rik, seal, med, motor end-plate disease, ataxia 3, nmf335, mnd2, mnd-2, nmf58, NaCh6, NMF335, Nav1.6
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 20273
Homologene: 7927
Mycbp2
Name: MYC binding protein 2, E3 ubiquitin protein ligase
Synonyms: Phr1, C130061D10Rik, Pam
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 105689
Homologene: 9005
Mid1
Name: midline 1
Synonyms: 61B3-R, Fxy, DXHXS1141, Trim18
Type: Gene
Species: Mouse
Chromosome: X
NCBI: 17318
HGNC: HGNC:7095
Homologene: 7837
Pdxdc1
Name: pyridoxal-dependent decarboxylase domain containing 1
Synonyms: 2210010A19Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 94184
Homologene: 22858
Chd9
Name: chromodomain helicase DNA binding protein 9
Synonyms: 1810014J18Rik, 9030205D12Rik, A330063D19Rik, AD013
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 109151
Homologene: 11844
Dhx15
Name: DEAH-box helicase 15
Synonyms: DEAH (Asp-Glu-Ala-His) box polypeptide 15, Ddx15, HRH2, DBP1, mDEAH9
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 13204
HGNC: HGNC:2738
Homologene: 1040
Smg6
Name: SMG6 nonsense mediated mRNA decay factor
Synonyms: Smg-6 homolog, nonsense mediated mRNA decay factor (C. elegans)
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 103677
Homologene: 23024
Cdc27
Name: cell division cycle 27
Synonyms: APC3
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 217232
HGNC: HGNC:1728
Homologene: 960
D5Ertd579e
Name: DNA segment, Chr 5, ERATO Doi 579, expressed
Synonyms: 9030221A05Rik, A930018H20Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 320661
Homologene: 19716
Cul1
Name: cullin 1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 26965
HGNC: HGNC:2551
Homologene: 2663
Mis18bp1
Name: MIS18 binding protein 1
Synonyms: C79407
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 217653
Homologene: 10147
Plekhm3
Name: pleckstrin homology domain containing, family M, member 3
Synonyms: A230102O09Rik, 9430067K14Rik, Plekhm1l
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 241075
Homologene: 18459
Trpm2
Name: transient receptor potential cation channel, subfamily M, member 2
Synonyms: TRPC7, Trrp7, 9830168K16Rik, LTRPC2
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 28240
Homologene: 20709
Dppa4
Name: developmental pluripotency associated 4
Synonyms: ECAT15-1, 2410091M23Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 73693
VEGA: 16
Homologene: 130462
Dnah9
Name: dynein, axonemal, heavy chain 9
Synonyms: Dnahc9, D11Ertd686e
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 237806
HGNC: HGNC:2953
Homologene: 20357
Umodl1
Name: uromodulin-like 1
Synonyms: D17Ertd488e
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 52020
VEGA: 17
Homologene: 45466
Ubtd2
Name: ubiquitin domain containing 2
Synonyms: 9630054F20Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 327900
Homologene: 15484
Plcl1
Name: phospholipase C-like 1
Synonyms: C230017K02Rik, PLC-L, PRIP-1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 227120
HGNC: HGNC:9063
Homologene: 38155
Or11j4
Name: olfactory receptor family 11 subfamily J member 4
Synonyms: MOR106-5, GA_x6K02T2PMLR-6089963-6090901, Olfr736
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 258660
Homologene: 64902
Klk1b11
Name: kallikrein 1-related peptidase b11
Synonyms: mGK-11, Klk11
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 16613
Homologene: 68141
Or52i2
Name: olfactory receptor family 52 subfamily J member 2
Synonyms: MOR41-1, GA_x6K02T2PBJ9-5386601-5387575, Olfr556
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 258749
Homologene: 17380
Or8h9
Name: olfactory receptor family 8 subfamily H member 9
Synonyms: Olfr1099, GA_x6K02T2Q125-48446067-48445129, MOR206-3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258764
Homologene: 107003
Or8d1b
Name: olfactory receptor family 8 subfamily D member 1B
Synonyms: MOR171-22, Olfr933, GA_x6K02T2PVTD-32671531-32672457
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 258433
VEGA: 9
HGNC: HGNC:8481
Homologene: 79676
Aim2
Name: absent in melanoma 2
Synonyms: Ifi210, LOC383619
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 383619
HGNC: HGNC:357
Homologene: 83226
Nfasc
Name: neurofascin
Synonyms: D430023G06Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 269116
Homologene: 24945
Cd46
Name: CD46 antigen, complement regulatory protein
Synonyms: CD46, Mcp
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 17221
HGNC: HGNC:6953
Homologene: 7832
Qrsl1
Name: glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1
Synonyms: GatA, 2700038P16Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 76563
Homologene: 6699
Ugt2b35
Name: UDP glucuronosyltransferase 2 family, polypeptide B35
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 243085
Homologene: 128251
Or52ac1
Name: olfactory receptor family 52 subfamily AC member 1
Synonyms: MOR38-1, Olfr655, GA_x6K02T2PBJ9-7224628-7223702
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 258817
Homologene: 17403
Sema4c
Name: sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4C
Synonyms: Semacl1, M-Sema F, Semacl1, Semai, Semaf
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 20353
Homologene: 23047
Adgra1
Name: adhesion G protein-coupled receptor A1
Synonyms: D7Ertd680e, 2900059M17Rik, Gpr123
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 52389
Homologene: 18582
Atat1
Name: alpha tubulin acetyltransferase 1
Synonyms: MEC-17, 0610011P08Rik, 3110080J08Rik, 2610008K08Rik, 2610110G12Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 73242
Homologene: 15129
Or4c124
Name: olfactory receptor family 4 subfamily C member 124
Synonyms: Olfr1232, MOR233-18, GA_x6K02T2Q125-50770831-50769896
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258320
Homologene: 74068
Cnn2
Name: calponin 2
Synonyms: h2-calponin, Calpo2
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 12798
HGNC: HGNC:2156
Homologene: 3215
Acsm4
Name: acyl-CoA synthetase medium-chain family member 4
Synonyms: OMACS, O-MACS
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233801
Homologene: 72281
Sars2
Name: seryl-aminoacyl-tRNA synthetase 2
Synonyms: D7Ertd353e, 2410015F05Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 71984
Homologene: 6073
Gja10
Name: gap junction protein, alpha 10
Synonyms: Cx59, connexin-57, Cx-57, Cx57
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 14610
Homologene: 7733
Grxcr2
Name: glutaredoxin, cysteine rich 2
Synonyms: LOC332309
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 332309
VEGA: 18
Homologene: 85304
Gm20481
Name: predicted gene 20481
Type: Gene
Species: Mouse
Chromosome: 17
P2ry10b
Name: purinergic receptor P2Y, G-protein coupled 10B
Synonyms: A630033H20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 213438
Homologene: 18448
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 36,553,756 bp
  • A to G, chromosome 1 at 55,696,886 bp
  • A to G, chromosome 1 at 64,921,889 bp
  • T to C, chromosome 1 at 132,634,671 bp
  • A to G, chromosome 1 at 173,455,444 bp
  • G to A, chromosome 1 at 195,085,360 bp
  • G to T, chromosome 2 at 19,446,281 bp
  • C to T, chromosome 2 at 86,958,699 bp
  • T to A, chromosome 2 at 89,325,253 bp
  • A to G, chromosome 4 at 32,601,313 bp
  • A to G, chromosome 5 at 36,616,470 bp
  • G to A, chromosome 5 at 52,166,732 bp
  • A to T, chromosome 5 at 87,003,378 bp
  • A to T, chromosome 5 at 125,300,345 bp
  • T to A, chromosome 6 at 47,508,989 bp
  • A to G, chromosome 6 at 128,491,240 bp
  • A to G, chromosome 7 at 28,750,093 bp
  • T to A, chromosome 7 at 43,995,911 bp
  • T to C, chromosome 7 at 102,670,049 bp
  • T to A, chromosome 7 at 104,596,639 bp
  • A to G, chromosome 7 at 119,698,574 bp
  • G to T, chromosome 7 at 139,852,521 bp
  • A to T, chromosome 8 at 90,977,680 bp
  • T to C, chromosome 9 at 38,976,086 bp
  • A to G, chromosome 10 at 43,882,162 bp
  • A to T, chromosome 10 at 77,923,593 bp
  • A to G, chromosome 10 at 79,991,442 bp
  • T to A, chromosome 11 at 32,499,406 bp
  • C to T, chromosome 11 at 66,027,082 bp
  • C to G, chromosome 11 at 74,990,141 bp
  • A to T, chromosome 11 at 104,517,395 bp
  • T to C, chromosome 12 at 65,140,757 bp
  • A to T, chromosome 14 at 50,392,912 bp
  • T to C, chromosome 14 at 103,155,658 bp
  • A to G, chromosome 15 at 100,957,091 bp
  • T to C, chromosome 16 at 13,837,126 bp
  • T to A, chromosome 16 at 48,289,336 bp
  • T to C, chromosome 17 at 30,994,815 bp
  • T to G, chromosome 17 at 34,972,133 bp
  • C to A, chromosome 17 at 35,897,771 bp
  • T to C, chromosome 18 at 41,986,544 bp
  • C to T, chromosome 19 at 52,997,024 bp
  • C to T, chromosome X at 107,171,118 bp
  • T to C, chromosome X at 169,927,425 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4452 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041713-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.