Strain Name:
C57BL/6J-MtgxR5214Btlr/Mmmh
Stock Number:
042787-MU
Citation ID:
RRID:MMRRC_042787-MU
Other Names:
R5214 (G1), C57BL/6J-MtgxR5214Btlr
Major Collection:

Strain Information

Dspp
Name: dentin sialophosphoprotein
Synonyms: Dmp3, Dpp, Dsp
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 666279
HGNC: HGNC:3054
Cdon
Name: cell adhesion molecule-related/down-regulated by oncogenes
Synonyms: CAM-related/down-regulated by oncogenes, CDO
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 57810
Homologene: 22996
Prnp
Name: prion protein
Synonyms: PrP, PrPC, Sinc, Prn-i, Prn-p, PrPSc, CD230
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 19122
HGNC: HGNC:9449
Homologene: 7904
Aldh1l1
Name: aldehyde dehydrogenase 1 family, member L1
Synonyms: 1810048F20Rik, Fthfd
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 107747
HGNC: HGNC:3978
Homologene: 122031
Nos2
Name: nitric oxide synthase 2, inducible
Synonyms: iNOS, Nos-2, NOS-II, Nos2a
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 18126
HGNC: HGNC:7873
Homologene: 55473
Ndrg1
Name: N-myc downstream regulated gene 1
Synonyms: Tdd5, PROXY1, CMT4D, CAP43, TDD5, DRG1, Ndr1, Ndrl
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 17988
HGNC: HGNC:7679
Homologene: 55953
Dlc1
Name: deleted in liver cancer 1
Synonyms: p122-RhoGAP, STARD12, Arhgap7, A730069N07Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 50768
HGNC: HGNC:2897
Homologene: 4442
Eps8
Name: epidermal growth factor receptor pathway substrate 8
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 13860
HGNC: HGNC:3420
Homologene: 3272
Atm
Name: ataxia telangiectasia mutated
Synonyms: C030026E19Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 11920
HGNC: HGNC:795
Homologene: 30952
Rabep1
Name: rabaptin, RAB GTPase binding effector protein 1
Synonyms: RAB5 effector protein, neurocrescin, rabaptin-5
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 54189
Homologene: 3451
Rlf
Name: rearranged L-myc fusion sequence
Synonyms: 9230110M18Rik, MommeD8
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 109263
Homologene: 8243
Ankrd17
Name: ankyrin repeat domain 17
Synonyms: 4933425K22Rik, Gtar, A130069E23Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 81702
Homologene: 82403
Ccar1
Name: cell division cycle and apoptosis regulator 1
Synonyms: Carp1, 2610511G16Rik, 9430036H15Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 67500
VEGA: 10
Homologene: 10086
Zfp280d
Name: zinc finger protein 280D
Synonyms: Suhw4
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235469
Homologene: 17151
Bltp3a
Name: bridge-like lipid transfer protein family member 3A
Synonyms: 1110020K19Rik, F830021D11Rik, Uhrf1bp1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 224648
Homologene: 9816
Ccdc14
Name: coiled-coil domain containing 14
Synonyms: G630039H03Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 239839
VEGA: 16
Homologene: 32549
Dock4
Name: dedicator of cytokinesis 4
Synonyms: EST N28122, 6330411N01Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 238130
VEGA: 12
Homologene: 56680
Ckap2l
Name: cytoskeleton associated protein 2-like
Synonyms: 2010016H04Rik, 2610318C08Rik, Radmis
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 70466
Homologene: 51866
Tomm70a
Name: translocase of outer mitochondrial membrane 70A
Synonyms: D16Ium22, D16Ium22e, 2610044B22Rik, Tomm70a, D16Wsu109e, Tom70
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 28185
VEGA: 16
Homologene: 40112
Cdc45
Name: cell division cycle 45
Synonyms: Cdc45l
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 12544
HGNC: HGNC:1739
Homologene: 2616
Adgrl1
Name: adhesion G protein-coupled receptor L1
Synonyms: Lec2, lectomedin-2, 2900070I05Rik, Lphn1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 330814
Homologene: 8951
Jcad
Name: junctional cadherin 5 associated
Synonyms: 9430020K01Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 240185
VEGA: 18
Homologene: 19370
Ppp6r1
Name: protein phosphatase 6, regulatory subunit 1
Synonyms: 2010309P17Rik, B430201G11Rik, Pp6r1, Saps1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 243819
Homologene: 86982
Raf1
Name: v-raf-leukemia viral oncogene 1
Synonyms: Raf-1, 6430402F14Rik, v-Raf, sarcoma 3611 oncogene, c-Raf, Craf1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 110157
HGNC: HGNC:9829
Homologene: 48145
Tbc1d19
Name: TBC1 domain family, member 19
Synonyms: 2810453K03Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 67249
Homologene: 10130
Zfhx4
Name: zinc finger homeodomain 4
Synonyms: Zfh-4, C130041O22Rik, Zfh4
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 80892
Homologene: 23477
Scaf1
Name: SR-related CTD-associated factor 1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233208
Tecta
Name: tectorin alpha
Synonyms: [a]-tectorin, Tctna
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 21683
Homologene: 3955
Sh3rf1
Name: SH3 domain containing ring finger 1
Synonyms: Posh, 2200003J05Rik, Sh3md2
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 59009
Homologene: 10988
Uba7
Name: ubiquitin-like modifier activating enzyme 7
Synonyms: 1300004C08Rik, Ube1l
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 74153
Homologene: 2502
Slc22a21
Name: solute carrier family 22 (organic cation transporter), member 21
Synonyms: Octn3, Slc22a9
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 56517
Homologene: 137336
Zscan20
Name: zinc finger and SCAN domains 20
Synonyms: Zfp31
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 269585
Homologene: 51463
Gm5114
Name: predicted gene 5114
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 330513
Homologene: 45597
Treh
Name: trehalase (brush-border membrane glycoprotein)
Synonyms: 2210412M19Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 58866
Homologene: 5198
Fras1
Name: Fraser extracellular matrix complex subunit 1
Synonyms: bl, E130113P14Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231470
Homologene: 23516
Or11g27
Name: olfactory receptor family 11 subfamily G member 27
Synonyms: GA_x6K02T2N6FY-3870-3385, GA_x6K02T2N6FY-2320-2039, GA_x6K02T2PMLR-6243196-6244132, MOR106-8P, MOR106-14, Olfr265, Olfr743-ps1, Olfr264, Olfr743
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 219019
Homologene: 74163
Tmc5
Name: transmembrane channel-like gene family 5
Synonyms: 4932443L08Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 74424
Homologene: 11713
Rnpepl1
Name: arginyl aminopeptidase (aminopeptidase B)-like 1
Synonyms: 1110014H17Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 108657
Homologene: 36367
Lrtm1
Name: leucine-rich repeats and transmembrane domains 1
Synonyms: A930016D02Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 319476
VEGA: 14
Homologene: 49635
Gm973
Name: predicted gene 973
Synonyms: LOC381260
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 381260
Homologene: 124277
Cacna1e
Name: calcium channel, voltage-dependent, R type, alpha 1E subunit
Synonyms: Cav2.3, Cchra1, alpha1E
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12290
HGNC: HGNC:1392
Homologene: 20185
Klk1b11
Name: kallikrein 1-related peptidase b11
Synonyms: mGK-11, Klk11
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 16613
Homologene: 68141
Them4
Name: thioesterase superfamily member 4
Synonyms: 4921507I02Rik, 2700077M13Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 75778
Homologene: 14208
Dennd2d
Name: DENN domain containing 2D
Synonyms: 2010308M01Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 72121
Homologene: 11776
Ttc28
Name: tetratricopeptide repeat domain 28
Synonyms: 2310015L07Rik, TPRBK
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 209683
Homologene: 41023
Tmf1
Name: TATA element modulatory factor 1
Synonyms: LOC232286, 7030402D04Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 232286
Homologene: 133801
Acsbg3
Name: acyl-CoA synthetase bubblegum family member 3
Synonyms: 1700061G19Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 78625
Homologene: 28378
Ube4a
Name: ubiquitination factor E4A
Synonyms: UFD2b, 9930123J21Rik, 4732444G18Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 140630
Homologene: 3517
Kcnh8
Name: potassium voltage-gated channel, subfamily H (eag-related), member 8
Synonyms: Kv12.1, ELK1, C130090D05Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 211468
Homologene: 14332
Ptprb
Name: protein tyrosine phosphatase receptor type B
Synonyms: 3230402H02Rik, VE-PTP
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 19263
VEGA: 10
HGNC: HGNC:9665
Homologene: 2125
Cntnap3
Name: contactin associated protein-like 3
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 238680
VEGA: 13
Homologene: 129602
Tmem8b
Name: transmembrane protein 8B
Synonyms: 4930500O05Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 242409
Homologene: 72894
Ldhb
Name: lactate dehydrogenase B
Synonyms: lactate dehydrogenase-B, Ldh-2, H-Ldh
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 16832
HGNC: HGNC:6541
Homologene: 55647
Lrrc34
Name: leucine rich repeat containing 34
Synonyms: 1700007J06Rik, Spata34
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 71827
Homologene: 27419
Pnpo
Name: pyridoxine 5'-phosphate oxidase
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 103711
Homologene: 5364
Zw10
Name: zw10 kinetochore protein
Synonyms: MmZw10, 6330566F14Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 26951
VEGA: 9
Homologene: 37959
Haus6
Name: HAUS augmin-like complex, subunit 6
Synonyms: D4Ertd27e, 6230416J20Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230376
Homologene: 9760
Tc2n
Name: tandem C2 domains, nuclear
Synonyms: Tac2-N, 4933406D09Rik, Mtac2d1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 74413
Homologene: 12560
Dph7
Name: diphthamine biosynethesis 7
Synonyms: 2810443J12Rik, Wdr85
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 67228
Homologene: 5432
Miga2
Name: mitoguardin 2
Synonyms: R74766, 5730472N09Rik, Fam73b
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 108958
Homologene: 13125
Arhgap27
Name: Rho GTPase activating protein 27
Synonyms: 2310069I04Rik, 5730442P18Rik, Sh3d20
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 544817
Homologene: 45715
Calhm5
Name: calcium homeostasis modulator family member 5
Synonyms: Fam26e
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 103511
VEGA: 10
Homologene: 17807
Il36a
Name: interleukin 36A
Synonyms: IL-1H1, Fil1, Fil1epsilon, Il1f6, If36a
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 54448
Homologene: 8688
Ccdc113
Name: coiled-coil domain containing 113
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 244608
Homologene: 40927
Tbx2
Name: T-box 2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 21385
Homologene: 38123
Syt15
Name: synaptotagmin XV
Synonyms: CHR10SYT, sytXV, E230025K04Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 319508
Homologene: 12901
1700061I17Rik
Name: RIKEN cDNA 1700061I17 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 433646
Herpud1
Name: homocysteine-inducible, endoplasmic reticulum stress-inducible, ubiquitin-like domain member 1
Synonyms: Herp, Mifl
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 64209
Homologene: 40973
Mageb3
Name: MAGE family member B3
Synonyms: Smage3, Mage-rs3, Mage-ps1, Mage-b3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 17147
Homologene: 137208
4930449A18Rik
Name: RIKEN cDNA 4930449A18 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Pcdhgb1
Name: protocadherin gamma subfamily B, 1
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 93699
HGNC: HGNC:8708
Homologene: 81867
Msantd5
Name: Myb/SANT DNA binding domain containing 5
Synonyms: Gm12569
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 622699
Homologene: 141209
Tmem200c
Name: transmembrane protein 200C
Synonyms: Gm6338
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 622645
VEGA: 17
Homologene: 78765
Mrpl33
Name: mitochondrial ribosomal protein L33
Synonyms: 0610009M10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 66845
Homologene: 81733
Gm17669
Name: predicted gene, 17669
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 108168363
VEGA: 18
4731419I09Rik
Name: RIKEN cDNA 4731419I09 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Cldn21
Name:
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 59,526,721 bp
  • G to T, chromosome 1 at 92,919,279 bp
  • T to A, chromosome 1 at 154,701,364 bp
  • C to T, chromosome 2 at 24,224,489 bp
  • C to T, chromosome 2 at 24,966,587 bp
  • A to G, chromosome 2 at 30,371,196 bp
  • T to C, chromosome 2 at 121,954,838 bp
  • T to C, chromosome 2 at 129,285,469 bp
  • C to T, chromosome 2 at 131,937,004 bp
  • A to C, chromosome 3 at 5,403,641 bp
  • A to T, chromosome 3 at 30,636,248 bp
  • A to T, chromosome 3 at 59,825,884 bp
  • G to A, chromosome 3 at 89,337,908 bp
  • A to G, chromosome 3 at 94,317,511 bp
  • G to A, chromosome 3 at 106,486,321 bp
  • A to G, chromosome 3 at 117,067,775 bp
  • G to A, chromosome 4 at 43,673,992 bp
  • A to G, chromosome 4 at 86,601,288 bp
  • T to C, chromosome 4 at 121,150,700 bp
  • G to A, chromosome 4 at 128,588,316 bp
  • A to G, chromosome 5 at 31,650,392 bp
  • T to C, chromosome 5 at 53,849,841 bp
  • T to C, chromosome 5 at 90,283,460 bp
  • T to C, chromosome 5 at 96,769,593 bp
  • A to G, chromosome 5 at 104,178,498 bp
  • A to G, chromosome 5 at 111,177,623 bp
  • A to G, chromosome 6 at 90,563,417 bp
  • G to C, chromosome 6 at 97,167,292 bp
  • G to T, chromosome 6 at 115,637,622 bp
  • A to T, chromosome 6 at 137,527,492 bp
  • T to A, chromosome 6 at 142,495,595 bp
  • C to T, chromosome 7 at 4,643,177 bp
  • G to T, chromosome 7 at 39,408,368 bp
  • C to A, chromosome 7 at 43,997,842 bp
  • G to A, chromosome 7 at 45,003,238 bp
  • C to T, chromosome 7 at 118,647,932 bp
  • G to T, chromosome 8 at 36,938,211 bp
  • A to T, chromosome 8 at 61,372,731 bp
  • G to A, chromosome 8 at 83,915,573 bp
  • G to T, chromosome 8 at 94,390,851 bp
  • T to C, chromosome 8 at 95,545,973 bp
  • T to C, chromosome 9 at 35,483,208 bp
  • C to T, chromosome 9 at 42,345,668 bp
  • T to C, chromosome 9 at 44,682,876 bp
  • T to C, chromosome 9 at 44,948,868 bp
  • CAAAAA to CAAAAAA, chromosome 9 at 49,048,082 bp
  • T to C, chromosome 9 at 49,064,163 bp
  • C to A, chromosome 9 at 53,491,027 bp
  • T to A, chromosome 9 at 72,308,113 bp
  • T to C, chromosome 9 at 107,977,514 bp
  • A to G, chromosome 10 at 34,092,491 bp
  • G to A, chromosome 10 at 62,770,961 bp
  • T to C, chromosome 10 at 116,369,324 bp
  • C to A, chromosome 11 at 51,234,848 bp
  • A to T, chromosome 11 at 53,953,043 bp
  • C to T, chromosome 11 at 70,900,522 bp
  • T to C, chromosome 11 at 78,955,441 bp
  • G to T, chromosome 11 at 85,838,437 bp
  • C to A, chromosome 11 at 96,942,469 bp
  • C to T, chromosome 11 at 103,334,033 bp
  • A to G, chromosome 12 at 40,704,466 bp
  • A to T, chromosome 12 at 101,693,202 bp
  • G to T, chromosome 13 at 64,762,010 bp
  • C to T, chromosome 14 at 29,021,694 bp
  • A to G, chromosome 14 at 34,221,746 bp
  • T to G, chromosome 14 at 50,534,347 bp
  • G to A, chromosome 15 at 66,959,390 bp
  • A to G, chromosome 15 at 76,177,721 bp
  • C to T, chromosome 16 at 18,795,897 bp
  • T to A, chromosome 16 at 34,704,855 bp
  • G to A, chromosome 16 at 57,121,937 bp
  • G to A, chromosome 17 at 27,887,515 bp
  • T to C, chromosome 17 at 52,898,458 bp
  • T to C, chromosome 17 at 56,886,493 bp
  • C to T, chromosome 17 at 68,841,127 bp
  • T to A, chromosome 18 at 4,674,134 bp
  • T to A, chromosome 18 at 37,681,425 bp
  • C to T, chromosome 18 at 67,562,409 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5214 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042787-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.